Click here to switch to the map view.

The map label for this gene is kdsB [H]

Identifier: 94314653

GI number: 94314653

Start: 2494108

End: 2494851

Strand: Direct

Name: kdsB [H]

Synonym: Rmet_5734

Alternate gene names: 94314653

Gene position: 2494108-2494851 (Clockwise)

Preceding gene: 94314652

Following gene: 94314654

Centisome position: 96.67

GC content: 60.62

Gene sequence:

>744_bases
ATGACACGGCAGGATGGCGAGAGCGTTGTCATCGTCATTCCCGCGCGCTACGGATCGACGCGCTTGCCTGGCAAGCCGTT
GCTGGATATTGCCGGCAAACCGATGATTCAGCACGTCTATGAGCGTGCACGTTTGGTGCGCGGCGTAAATGCCGTGGTGG
TTGCCACGGATGACTCGCGCATCGAGTCGGCGGTTCTGGCATTCGGCGGGAGCTGTCTGATGACGTCCGCCGGGCACCAG
TCGGGAACTGACCGCCTTTGCGAAGTCATGCGCATGGTGCACGCGGACCTCTACATCAACTTGCAGGGTGACGAGCCGCT
GGTGCGTCCAACGGACATCGAACTGCTGGCGAACCTGATGATCGCCGATCCGACCTCCGACGTAGGCACGCTTTGCCATG
AGTTCTCGCTGGAGGAAGCCCGCGACGAAAATGCCGTGAAGGTGGTGATGTCGAACGAAGGTGATGCGCTGTACTTCAGT
CGCTCATTGATCCCCTACCCGCGCGATGGCGCCTTTGCGCGCTACTACAAGCATTCGGGTGTCTACGCTTTTCGGAGAAG
GGTGCTTGAGGTCTACGGGAGCATGCCCCAGCCGATGGCCGAACAAGCGGAGCGGTTGGAGCAATTGAGGCTGCTTGCGG
CAGGCTTCCGCATACGGATGCAGGTGGTGCCTCCCATCGGAACTGGTGTCGATACTCCCGCTTGCCTGGAGCGTGTCCGG
GCCATTCTTGATCCCGCTGTTTGA

Upstream 100 bases:

>100_bases
GCCCGAGTGCACTGCCACTGGACCAACTTGAGCCCTTCCTCACCCAGGTAAAGATGCTCGACGATGTTGTGAAATCGCTG
CCGGCAACGGAGCTCGCCTG

Downstream 100 bases:

>100_bases
CGGCAGCTGAAGATCTACAGACAAAAGGACAAACGGAACATGCCAGAGTCAATTTCTCTTGCAAAGCAAGTGGTAGCCAC
GGAAATCCGCGCGCTGGAGG

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MTRQDGESVVIVIPARYGSTRLPGKPLLDIAGKPMIQHVYERARLVRGVNAVVVATDDSRIESAVLAFGGSCLMTSAGHQ
SGTDRLCEVMRMVHADLYINLQGDEPLVRPTDIELLANLMIADPTSDVGTLCHEFSLEEARDENAVKVVMSNEGDALYFS
RSLIPYPRDGAFARYYKHSGVYAFRRRVLEVYGSMPQPMAEQAERLEQLRLLAAGFRIRMQVVPPIGTGVDTPACLERVR
AILDPAV

Sequences:

>Translated_247_residues
MTRQDGESVVIVIPARYGSTRLPGKPLLDIAGKPMIQHVYERARLVRGVNAVVVATDDSRIESAVLAFGGSCLMTSAGHQ
SGTDRLCEVMRMVHADLYINLQGDEPLVRPTDIELLANLMIADPTSDVGTLCHEFSLEEARDENAVKVVMSNEGDALYFS
RSLIPYPRDGAFARYYKHSGVYAFRRRVLEVYGSMPQPMAEQAERLEQLRLLAAGFRIRMQVVPPIGTGVDTPACLERVR
AILDPAV
>Mature_246_residues
TRQDGESVVIVIPARYGSTRLPGKPLLDIAGKPMIQHVYERARLVRGVNAVVVATDDSRIESAVLAFGGSCLMTSAGHQS
GTDRLCEVMRMVHADLYINLQGDEPLVRPTDIELLANLMIADPTSDVGTLCHEFSLEEARDENAVKVVMSNEGDALYFSR
SLIPYPRDGAFARYYKHSGVYAFRRRVLEVYGSMPQPMAEQAERLEQLRLLAAGFRIRMQVVPPIGTGVDTPACLERVRA
ILDPAV

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria [H]

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family [H]

Homologues:

Organism=Escherichia coli, GI1787147, Length=248, Percent_Identity=47.5806451612903, Blast_Score=181, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003329
- InterPro:   IPR004528 [H]

Pfam domain/function: PF02348 CTP_transf_3 [H]

EC number: =2.7.7.38 [H]

Molecular weight: Translated: 27245; Mature: 27114

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRQDGESVVIVIPARYGSTRLPGKPLLDIAGKPMIQHVYERARLVRGVNAVVVATDDSR
CCCCCCCEEEEEEECCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCHH
IESAVLAFGGSCLMTSAGHQSGTDRLCEVMRMVHADLYINLQGDEPLVRPTDIELLANLM
HHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHH
IADPTSDVGTLCHEFSLEEARDENAVKVVMSNEGDALYFSRSLIPYPRDGAFARYYKHSG
HCCCCCHHHHHHHHHCHHHHCCCCEEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHCC
VYAFRRRVLEVYGSMPQPMAEQAERLEQLRLLAAGFRIRMQVVPPIGTGVDTPACLERVR
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCHHHHHHHH
AILDPAV
HHHCCCC
>Mature Secondary Structure 
TRQDGESVVIVIPARYGSTRLPGKPLLDIAGKPMIQHVYERARLVRGVNAVVVATDDSR
CCCCCCEEEEEEECCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCHH
IESAVLAFGGSCLMTSAGHQSGTDRLCEVMRMVHADLYINLQGDEPLVRPTDIELLANLM
HHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHH
IADPTSDVGTLCHEFSLEEARDENAVKVVMSNEGDALYFSRSLIPYPRDGAFARYYKHSG
HCCCCCHHHHHHHHHCHHHHCCCCEEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHCC
VYAFRRRVLEVYGSMPQPMAEQAERLEQLRLLAAGFRIRMQVVPPIGTGVDTPACLERVR
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCHHHHHHHH
AILDPAV
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8397187; 7875563; 8706906 [H]