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The map label for this gene is cynT [H]

Identifier: 94314524

GI number: 94314524

Start: 2360826

End: 2361476

Strand: Reverse

Name: cynT [H]

Synonym: Rmet_5605

Alternate gene names: 94314524

Gene position: 2361476-2360826 (Counterclockwise)

Preceding gene: 94314526

Following gene: 94314521

Centisome position: 91.53

GC content: 64.52

Gene sequence:

>651_bases
GTGAAAGATCTGATCCAGGGCATTCTGAAGTTCCAGCGGGAAGAATTCCCGAAGCGGTCGGCGTTGTTCAAGCACCTGGC
CACGCAGCAGAATCCCAAGACGTTGTTCATCGCGTGTTCGGACAGCCGCGTCGTGCCGGCGTTGCTGACACAGTGCGAGC
CCGGGGAGCTGTTCGTGATCCGCAACGCCGGCAATATCGTCCCGGCCTACGCGGTGCAGCCAGGCGGCGTGTCCGCCAGC
GTCGAATACGCGGTGGCGGGGCTCAAGGTGCGCGACATCGTGATCTGTGGCCATTCCGATTGCGGGGCCATGACGGCCGT
GGCCACCTGCCAATGCCTGGACCACATGCCTGCTGTCGAAGAGTGGCTCGAACATGCCAGCGGCGCCCGGCGGATCAGTC
TGACCCGTCCGCACGCATCGGACCGGGCACGGGTGGACGACATGGTGCGTGAGAACGTGATCGAGCAGCTTGACCACCTG
CGGACGCATCCGTCGGTGGCGCAGGCGCTGGCCGAAGGGCGTGTCGACCTGCATGGCTGGGTCTACGACATTGAAACCGG
TGAGATCGATGCCCTCGAAGGACGTACCGGGAAGTTCGTGCCGCTGCGGGAACAGCCTGATGTCACCGCCACGCTGGAGC
GGGTTGCCTGA

Upstream 100 bases:

>100_bases
ACTATTGGTGTTGCCAATGACGAGGATAATAAACCGGTCTTTGACCCAATAGCTGTTCGCCGCCATGCTGGAGCCATCCA
ATGACGATGGGGGCGTCGGC

Downstream 100 bases:

>100_bases
CGCCAGGGATTGGCAGGTATCAGCGCGAGGGCGATTGCGCAGGCGCCGCGCGCAGCACGCCTGCGGCGATTTCGCGCATC
AGCTCATCGACGGTCTCGAG

Product: carbonic anhydrase

Products: NA

Alternate protein names: Carbonate dehydratase 1 [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MKDLIQGILKFQREEFPKRSALFKHLATQQNPKTLFIACSDSRVVPALLTQCEPGELFVIRNAGNIVPAYAVQPGGVSAS
VEYAVAGLKVRDIVICGHSDCGAMTAVATCQCLDHMPAVEEWLEHASGARRISLTRPHASDRARVDDMVRENVIEQLDHL
RTHPSVAQALAEGRVDLHGWVYDIETGEIDALEGRTGKFVPLREQPDVTATLERVA

Sequences:

>Translated_216_residues
MKDLIQGILKFQREEFPKRSALFKHLATQQNPKTLFIACSDSRVVPALLTQCEPGELFVIRNAGNIVPAYAVQPGGVSAS
VEYAVAGLKVRDIVICGHSDCGAMTAVATCQCLDHMPAVEEWLEHASGARRISLTRPHASDRARVDDMVRENVIEQLDHL
RTHPSVAQALAEGRVDLHGWVYDIETGEIDALEGRTGKFVPLREQPDVTATLERVA
>Mature_216_residues
MKDLIQGILKFQREEFPKRSALFKHLATQQNPKTLFIACSDSRVVPALLTQCEPGELFVIRNAGNIVPAYAVQPGGVSAS
VEYAVAGLKVRDIVICGHSDCGAMTAVATCQCLDHMPAVEEWLEHASGARRISLTRPHASDRARVDDMVRENVIEQLDHL
RTHPSVAQALAEGRVDLHGWVYDIETGEIDALEGRTGKFVPLREQPDVTATLERVA

Specific function: Reversible hydration of carbon dioxide. Carbon dioxide formed in the bicarbonate-dependent decomposition of cyanate by cyanase (CynS) diffuses out of the cell faster than it would be hydrated to bicarbonate, so the apparent function of this enzyme is to c

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family [H]

Homologues:

Organism=Escherichia coli, GI1786534, Length=210, Percent_Identity=67.6190476190476, Blast_Score=303, Evalue=6e-84,
Organism=Escherichia coli, GI1786318, Length=187, Percent_Identity=36.3636363636364, Blast_Score=93, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI115532990, Length=252, Percent_Identity=25.3968253968254, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI25147564, Length=242, Percent_Identity=28.099173553719, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324292, Length=208, Percent_Identity=27.8846153846154, Blast_Score=84, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24645213, Length=240, Percent_Identity=24.1666666666667, Blast_Score=84, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001765
- InterPro:   IPR015892 [H]

Pfam domain/function: PF00484 Pro_CA [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 23732; Mature: 23732

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00704 PROK_CO2_ANHYDRASE_1 ; PS00705 PROK_CO2_ANHYDRASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLIQGILKFQREEFPKRSALFKHLATQQNPKTLFIACSDSRVVPALLTQCEPGELFVI
CHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHCCCCCCEEEE
RNAGNIVPAYAVQPGGVSASVEYAVAGLKVRDIVICGHSDCGAMTAVATCQCLDHMPAVE
ECCCCCCEEEEECCCCCCCCHHHHHCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCHHHH
EWLEHASGARRISLTRPHASDRARVDDMVRENVIEQLDHLRTHPSVAQALAEGRVDLHGW
HHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEECEE
VYDIETGEIDALEGRTGKFVPLREQPDVTATLERVA
EEEECCCCEEECCCCCCCEEECCCCCCHHHHHHHCC
>Mature Secondary Structure
MKDLIQGILKFQREEFPKRSALFKHLATQQNPKTLFIACSDSRVVPALLTQCEPGELFVI
CHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHCCCCCCEEEE
RNAGNIVPAYAVQPGGVSASVEYAVAGLKVRDIVICGHSDCGAMTAVATCQCLDHMPAVE
ECCCCCCEEEEECCCCCCCCHHHHHCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCHHHH
EWLEHASGARRISLTRPHASDRARVDDMVRENVIEQLDHLRTHPSVAQALAEGRVDLHGW
HHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEECEE
VYDIETGEIDALEGRTGKFVPLREQPDVTATLERVA
EEEECCCCEEECCCCCCCEEECCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]