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The map label for this gene is dadA [H]

Identifier: 94314499

GI number: 94314499

Start: 2333000

End: 2334268

Strand: Direct

Name: dadA [H]

Synonym: Rmet_5580

Alternate gene names: 94314499

Gene position: 2333000-2334268 (Clockwise)

Preceding gene: 94314498

Following gene: 94314501

Centisome position: 90.42

GC content: 65.96

Gene sequence:

>1269_bases
ATGAAATCCATCGCAGTGATCGGCGGCGGCATCACCGGCGCCACGACGGCTTACGCCCTGGCCAAACGCGGGTTCTCCGT
CACCCTGTTCGAACGGCACCGCTATGCGGCCATGGAAACCTCGTTCGCCAACGGCGGACAGCTCTCCGCCTCCAACGCGG
AAGTCTGGACGCACTGGTCCACGCTGGCCAAGGGCCTGAAGTGGATGCTGCGCAACGACGCGCCGCTGCTCGTCAACCCG
CGCCCGACCTGGCACAAGCTGAGCTGGTTCGCCGAGTTCATCGCATCGATCCCGCATTACCGCGGCAACACGGTGGAGAC
CACGCGGCTTGCCATTGCCGCGCGCCAGCACCTGTTTGCGTGGGCGGAGGCGGAGGGCATCGACTTCGACCTCAAGCGGC
GCGGCATCCTGCATATCTATCGGGACAAGGCGGGCTTCGATCACGCAGGGCAAGTCTCGAAGCTCCTGGCCGAAGGCGGA
CTCGAACGGCGCGCGGTGACCCCGGACGAGATGCGAAGCATCGAGCCGACGCTGGCAGGCCAGTACTATGGCGGCTACTA
CACGGAAAGCGACTCTACCGGCGACATCCACAAGTTCACCACCGGTATCGCTGCGGCGGCGCGCAGACTGGGTGTCCAGA
TGCTGTACAACCAGGACGTGAGCGCCGTGTCGACCAGCGGCCGGCGAGTGACGATCGTCTCGGCTGACGATGGCCAGACG
ACATCGCGCGAATTCGACGCAGTGGTCGTCTGCGCCGGCGTTGCCAGCCGCGATTTCGCGCGGCAACTCGGAGACCGCGT
GAACATCTATCCGGTCAAGGGCTATTCGATCACGGTCAACCTCAACGATGCCGAAAGCCAGGCCTCGGCGCCCACGGTCA
GCCTGCTCGACGACGAAACCAAGCTTGTCACCAGCCGTCTGGGCGTGGACCGATTCCGCATAGCCGGCACCGCCGAGTTC
AACGGCGTCGACCGCGACATCCGCGCCGATCGCATTCGTCCGCTGATCGACTGGGTCCATGCTTGCTTCCCCGGCGTCAG
CACCCGTAGCGTGGTGCCCTGGGCGGGGTTGCGGCCGATGATGCCGAACATGATGCCGCGCGTTGGCCGGGGCCATGCCG
CTAACGTGTTCTACAACACAGGGCATGGGCATCTCGGCTGGACGCTGTCCGCCGTGACGGCCGAGATGGTGGGGGATGTC
GTGCGCCACGCCGCCACGGCGCAGCAAGGGCGCGTGGGCGTTGGCGGATTGGTGGTGGGCAACGGCTGA

Upstream 100 bases:

>100_bases
TGTGCCGCGAATCTCTCAGGTCAAGGACAGAGGGGGCGTCCGCAACCGCGCATGGTGCGCGGCGGACTGCCATCCCACGA
CGTCCTGAAAGACCTTCGAC

Downstream 100 bases:

>100_bases
GGGAAGCCCTTCTTTCCGGGCCAAAAACGAAAAAAGCCCCGCTTGCGCGGGGCTTTTCGAATCTGGTGCCCAGAAGAGGA
CTCGAACCTCCACAGTGTTG

Product: D-amino acid dehydrogenase small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKSIAVIGGGITGATTAYALAKRGFSVTLFERHRYAAMETSFANGGQLSASNAEVWTHWSTLAKGLKWMLRNDAPLLVNP
RPTWHKLSWFAEFIASIPHYRGNTVETTRLAIAARQHLFAWAEAEGIDFDLKRRGILHIYRDKAGFDHAGQVSKLLAEGG
LERRAVTPDEMRSIEPTLAGQYYGGYYTESDSTGDIHKFTTGIAAAARRLGVQMLYNQDVSAVSTSGRRVTIVSADDGQT
TSREFDAVVVCAGVASRDFARQLGDRVNIYPVKGYSITVNLNDAESQASAPTVSLLDDETKLVTSRLGVDRFRIAGTAEF
NGVDRDIRADRIRPLIDWVHACFPGVSTRSVVPWAGLRPMMPNMMPRVGRGHAANVFYNTGHGHLGWTLSAVTAEMVGDV
VRHAATAQQGRVGVGGLVVGNG

Sequences:

>Translated_422_residues
MKSIAVIGGGITGATTAYALAKRGFSVTLFERHRYAAMETSFANGGQLSASNAEVWTHWSTLAKGLKWMLRNDAPLLVNP
RPTWHKLSWFAEFIASIPHYRGNTVETTRLAIAARQHLFAWAEAEGIDFDLKRRGILHIYRDKAGFDHAGQVSKLLAEGG
LERRAVTPDEMRSIEPTLAGQYYGGYYTESDSTGDIHKFTTGIAAAARRLGVQMLYNQDVSAVSTSGRRVTIVSADDGQT
TSREFDAVVVCAGVASRDFARQLGDRVNIYPVKGYSITVNLNDAESQASAPTVSLLDDETKLVTSRLGVDRFRIAGTAEF
NGVDRDIRADRIRPLIDWVHACFPGVSTRSVVPWAGLRPMMPNMMPRVGRGHAANVFYNTGHGHLGWTLSAVTAEMVGDV
VRHAATAQQGRVGVGGLVVGNG
>Mature_422_residues
MKSIAVIGGGITGATTAYALAKRGFSVTLFERHRYAAMETSFANGGQLSASNAEVWTHWSTLAKGLKWMLRNDAPLLVNP
RPTWHKLSWFAEFIASIPHYRGNTVETTRLAIAARQHLFAWAEAEGIDFDLKRRGILHIYRDKAGFDHAGQVSKLLAEGG
LERRAVTPDEMRSIEPTLAGQYYGGYYTESDSTGDIHKFTTGIAAAARRLGVQMLYNQDVSAVSTSGRRVTIVSADDGQT
TSREFDAVVVCAGVASRDFARQLGDRVNIYPVKGYSITVNLNDAESQASAPTVSLLDDETKLVTSRLGVDRFRIAGTAEF
NGVDRDIRADRIRPLIDWVHACFPGVSTRSVVPWAGLRPMMPNMMPRVGRGHAANVFYNTGHGHLGWTLSAVTAEMVGDV
VRHAATAQQGRVGVGGLVVGNG

Specific function: Oxidative deamination of D-amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=404, Percent_Identity=29.9504950495049, Blast_Score=190, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023080
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.99.1 [H]

Molecular weight: Translated: 45864; Mature: 45864

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIAVIGGGITGATTAYALAKRGFSVTLFERHRYAAMETSFANGGQLSASNAEVWTHWS
CCCEEEEECCCCHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCCEEECCCCHHHHHHH
TLAKGLKWMLRNDAPLLVNPRPTWHKLSWFAEFIASIPHYRGNTVETTRLAIAARQHLFA
HHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHE
WAEAEGIDFDLKRRGILHIYRDKAGFDHAGQVSKLLAEGGLERRAVTPDEMRSIEPTLAG
EECCCCCCEECCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHCC
QYYGGYYTESDSTGDIHKFTTGIAAAARRLGVQMLYNQDVSAVSTSGRRVTIVSADDGQT
CCCCCEEECCCCCCCHHHHHHHHHHHHHHHCHHEEECCCCHHHCCCCCEEEEEECCCCCC
TSREFDAVVVCAGVASRDFARQLGDRVNIYPVKGYSITVNLNDAESQASAPTVSLLDDET
CCCCCCEEEEECCCHHHHHHHHHCCCEEEEEECCEEEEEEECCCCCCCCCCEEEEECCHH
KLVTSRLGVDRFRIAGTAEFNGVDRDIRADRIRPLIDWVHACFPGVSTRSVVPWAGLRPM
HHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
MPNMMPRVGRGHAANVFYNTGHGHLGWTLSAVTAEMVGDVVRHAATAQQGRVGVGGLVVG
CHHHHHHCCCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
NG
CC
>Mature Secondary Structure
MKSIAVIGGGITGATTAYALAKRGFSVTLFERHRYAAMETSFANGGQLSASNAEVWTHWS
CCCEEEEECCCCHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCCEEECCCCHHHHHHH
TLAKGLKWMLRNDAPLLVNPRPTWHKLSWFAEFIASIPHYRGNTVETTRLAIAARQHLFA
HHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHE
WAEAEGIDFDLKRRGILHIYRDKAGFDHAGQVSKLLAEGGLERRAVTPDEMRSIEPTLAG
EECCCCCCEECCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHCC
QYYGGYYTESDSTGDIHKFTTGIAAAARRLGVQMLYNQDVSAVSTSGRRVTIVSADDGQT
CCCCCEEECCCCCCCHHHHHHHHHHHHHHHCHHEEECCCCHHHCCCCCEEEEEECCCCCC
TSREFDAVVVCAGVASRDFARQLGDRVNIYPVKGYSITVNLNDAESQASAPTVSLLDDET
CCCCCCEEEEECCCHHHHHHHHHCCCEEEEEECCEEEEEEECCCCCCCCCCEEEEECCHH
KLVTSRLGVDRFRIAGTAEFNGVDRDIRADRIRPLIDWVHACFPGVSTRSVVPWAGLRPM
HHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
MPNMMPRVGRGHAANVFYNTGHGHLGWTLSAVTAEMVGDVVRHAATAQQGRVGVGGLVVG
CHHHHHHCCCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
NG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]