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The map label for this gene is kstD [H]

Identifier: 94314474

GI number: 94314474

Start: 2302271

End: 2303938

Strand: Direct

Name: kstD [H]

Synonym: Rmet_5555

Alternate gene names: 94314474

Gene position: 2302271-2303938 (Clockwise)

Preceding gene: 94314473

Following gene: 94314475

Centisome position: 89.23

GC content: 59.35

Gene sequence:

>1668_bases
ATGACGGCCGCGCTGGTGGCCGCCCAACTCGGACTCAGCGTCGTGATTTGCGAAGCCACCGAACAGGTCGGCGGCACCAC
ATCTACTTCGGCTGGAACGTTGTGGCTACCAGGAAATCGCCATGGCCTCCAAGCCGGCCATGGCGACAGCACCGCTGCAG
GCAGCCAATACCTAGACGCGCTGATCGGGCCCGATGACCATCTCGGGCGGAGGAACGCATTTCTCGAAAGCGCTGGGCCG
GCCATCGAGTTCCTCGAGACGCAATGCGGACTGGCCTTCGTTAGCTCCGGCCAGCACCCGGACTATCTGGACCTCCCCGG
CGCCGCCGTCTACGGACGAGCGATATCGCCCAAAGAGTTTGACGGACGCCTGCTTCGTGCAGATTTCGACCGTGTGCGCC
CTCCCCTCAAGGACTTTCTTGTCCTCGGTGGCATGATGGCGAACAAGGCCGACGTCCTGGCACTTCTCGGCCGCTATCGC
TCGTGGCCAGCATTCAGGCGAACGGTTCAATTGGTCTTACGCTATCTCCGCGACAGGATTTCCTATGCACGAGGAACTCG
CCTTGTCATGGGTAACGCTTTGGTCGGACGAATGCTCTTTCGCTTGCGGCACTCAGGTGTGGAGATCCGATTCGGTACGA
GGCTACTCTCGATTGAAAAGTCGGGCAATCGGGTATCCGGCATCGTAGTCGAACGAAACGGCGTTACCTCAAGCATCAGC
GCGCGTCTCGGCGTAGTTCTCGCGACCGGCGGAATTGGGCACAACGAAAAGCTACGCCGTGACCTGCCGACCGGAGCCGT
CGAATCGCTCGCCCCTCCGTCCGTACGGGGCGATGCACTAGCCGCAGCGCAGGATGTCGGAGCAATCATCGAACGCCAGG
CGCGTGACTTCCTGTGGCAACCGGTCTCCCGAGTCCCAGACGGAAACGGTGGATGGCGCTTGTTTCCCCATCTCTACCTC
GACCGCGCTAAACCTGGTCTCATCGCAGTGAATTCGGCGGGGCAGCGATTCGTCAACGAGGGTGCGTCATATCACCACTT
CGCGGAATGCATGATGGGTTCCGGCAAGACTCTGGATGAAAACTTGCCTGCCTATCTCGTATGCGACCACCAGTTCATTC
AGAAATATGGGATCGGCGTTGTCCCTCCAGGCTGTCGCAATTTAAGCCCGTTCAAGCGATCCGGATACTTGGTTTCCGCA
GGTTCTATCTCTGAGCTTGCGCAGAAGATCGGCGTCAGCCCTTTTGGACTGTCGGCGACACTGCAGCAATACAACGCCGA
CGCTCTTGACGGCAAGGACAGCGCCTTCGGAAAAGGCGAGACTTCGGTCAGTCGATTCAACGGCGACGCAAGCAAACATC
CCAACCCATGCCTAGGTCCGGTCCAAGCGCCACCATTCTATGCGGTCCAAGTGTTCCCTGCTGACGCGGCAAGTTGTACG
GGCCTTCGGACGGACAAAGATGCGCGAGTCCTTGACGACACGGAAACCTCCATCGATGGACTATACGCATGTGGCAACGA
CATGGCGTCTGTCTTAAAGGGAGCCTACCCTGGCCCGGGCGCTACGCTGGGACCGGCCATCGTGTTCGCTTACCGCGCTG
CGCTTCATGCACAACGGAAAACGTTGACATCGTCCCAAAAAGAAGCGCGAGTCTATGAGGAGCAATAG

Upstream 100 bases:

>100_bases
TGCTGCAGTGGCAACTGGCCCCCGTCAGTTCGATCGACTGAGCAGCACTGCAGATATGACTACTTACGATCTCGTAGTCT
GCGGCGCAGGAGCGGGTGGC

Downstream 100 bases:

>100_bases
AGCAATGCTGAAACCTGAGCACGGTCGTGGACCTCTCAATGGAGTGCGCGTTGTCGAGTTTGCAGGGCTAGGACCTGCAC
CGTTTGCCTGCATGATGCTG

Product: putative FAD-binding dehydrogenase

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MTAALVAAQLGLSVVICEATEQVGGTTSTSAGTLWLPGNRHGLQAGHGDSTAAGSQYLDALIGPDDHLGRRNAFLESAGP
AIEFLETQCGLAFVSSGQHPDYLDLPGAAVYGRAISPKEFDGRLLRADFDRVRPPLKDFLVLGGMMANKADVLALLGRYR
SWPAFRRTVQLVLRYLRDRISYARGTRLVMGNALVGRMLFRLRHSGVEIRFGTRLLSIEKSGNRVSGIVVERNGVTSSIS
ARLGVVLATGGIGHNEKLRRDLPTGAVESLAPPSVRGDALAAAQDVGAIIERQARDFLWQPVSRVPDGNGGWRLFPHLYL
DRAKPGLIAVNSAGQRFVNEGASYHHFAECMMGSGKTLDENLPAYLVCDHQFIQKYGIGVVPPGCRNLSPFKRSGYLVSA
GSISELAQKIGVSPFGLSATLQQYNADALDGKDSAFGKGETSVSRFNGDASKHPNPCLGPVQAPPFYAVQVFPADAASCT
GLRTDKDARVLDDTETSIDGLYACGNDMASVLKGAYPGPGATLGPAIVFAYRAALHAQRKTLTSSQKEARVYEEQ

Sequences:

>Translated_555_residues
MTAALVAAQLGLSVVICEATEQVGGTTSTSAGTLWLPGNRHGLQAGHGDSTAAGSQYLDALIGPDDHLGRRNAFLESAGP
AIEFLETQCGLAFVSSGQHPDYLDLPGAAVYGRAISPKEFDGRLLRADFDRVRPPLKDFLVLGGMMANKADVLALLGRYR
SWPAFRRTVQLVLRYLRDRISYARGTRLVMGNALVGRMLFRLRHSGVEIRFGTRLLSIEKSGNRVSGIVVERNGVTSSIS
ARLGVVLATGGIGHNEKLRRDLPTGAVESLAPPSVRGDALAAAQDVGAIIERQARDFLWQPVSRVPDGNGGWRLFPHLYL
DRAKPGLIAVNSAGQRFVNEGASYHHFAECMMGSGKTLDENLPAYLVCDHQFIQKYGIGVVPPGCRNLSPFKRSGYLVSA
GSISELAQKIGVSPFGLSATLQQYNADALDGKDSAFGKGETSVSRFNGDASKHPNPCLGPVQAPPFYAVQVFPADAASCT
GLRTDKDARVLDDTETSIDGLYACGNDMASVLKGAYPGPGATLGPAIVFAYRAALHAQRKTLTSSQKEARVYEEQ
>Mature_554_residues
TAALVAAQLGLSVVICEATEQVGGTTSTSAGTLWLPGNRHGLQAGHGDSTAAGSQYLDALIGPDDHLGRRNAFLESAGPA
IEFLETQCGLAFVSSGQHPDYLDLPGAAVYGRAISPKEFDGRLLRADFDRVRPPLKDFLVLGGMMANKADVLALLGRYRS
WPAFRRTVQLVLRYLRDRISYARGTRLVMGNALVGRMLFRLRHSGVEIRFGTRLLSIEKSGNRVSGIVVERNGVTSSISA
RLGVVLATGGIGHNEKLRRDLPTGAVESLAPPSVRGDALAAAQDVGAIIERQARDFLWQPVSRVPDGNGGWRLFPHLYLD
RAKPGLIAVNSAGQRFVNEGASYHHFAECMMGSGKTLDENLPAYLVCDHQFIQKYGIGVVPPGCRNLSPFKRSGYLVSAG
SISELAQKIGVSPFGLSATLQQYNADALDGKDSAFGKGETSVSRFNGDASKHPNPCLGPVQAPPFYAVQVFPADAASCTG
LRTDKDARVLDDTETSIDGLYACGNDMASVLKGAYPGPGATLGPAIVFAYRAALHAQRKTLTSSQKEARVYEEQ

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 59313; Mature: 59182

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAALVAAQLGLSVVICEATEQVGGTTSTSAGTLWLPGNRHGLQAGHGDSTAAGSQYLDA
CCHHHHHHHHCCEEEEEECHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHH
LIGPDDHLGRRNAFLESAGPAIEFLETQCGLAFVSSGQHPDYLDLPGAAVYGRAISPKEF
HCCCCHHCCHHHHHHHCCCCHHHHHHHHCCCCEECCCCCCCCCCCCCHHHCCCCCCCCCC
DGRLLRADFDRVRPPLKDFLVLGGMMANKADVLALLGRYRSWPAFRRTVQLVLRYLRDRI
CCEEHEHHHHHHCCCHHHHHHHCCHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
SYARGTRLVMGNALVGRMLFRLRHSGVEIRFGTRLLSIEKSGNRVSGIVVERNGVTSSIS
HHHCCCEEEECHHHHHHHHHHHHHCCEEEEECCEEEEEECCCCEEEEEEEECCCCCHHHH
ARLGVVLATGGIGHNEKLRRDLPTGAVESLAPPSVRGDALAAAQDVGAIIERQARDFLWQ
HCEEEEEEECCCCCCHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
PVSRVPDGNGGWRLFPHLYLDRAKPGLIAVNSAGQRFVNEGASYHHFAECMMGSGKTLDE
HHHHCCCCCCCEEECHHHHHHCCCCCEEEECCCHHHHHHCCCCHHHHHHHHCCCCCCCCC
NLPAYLVCDHQFIQKYGIGVVPPGCRNLSPFKRSGYLVSAGSISELAQKIGVSPFGLSAT
CCCEEEEECHHHHHHCCCCCCCCCCCCCCCHHHCCEEEECCCHHHHHHHHCCCCCCHHHH
LQQYNADALDGKDSAFGKGETSVSRFNGDASKHPNPCLGPVQAPPFYAVQVFPADAASCT
HHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCC
GLRTDKDARVLDDTETSIDGLYACGNDMASVLKGAYPGPGATLGPAIVFAYRAALHAQRK
CCCCCCCCCEECCCCCCHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
TLTSSQKEARVYEEQ
HHHCCHHHHHHCCCC
>Mature Secondary Structure 
TAALVAAQLGLSVVICEATEQVGGTTSTSAGTLWLPGNRHGLQAGHGDSTAAGSQYLDA
CHHHHHHHHCCEEEEEECHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHH
LIGPDDHLGRRNAFLESAGPAIEFLETQCGLAFVSSGQHPDYLDLPGAAVYGRAISPKEF
HCCCCHHCCHHHHHHHCCCCHHHHHHHHCCCCEECCCCCCCCCCCCCHHHCCCCCCCCCC
DGRLLRADFDRVRPPLKDFLVLGGMMANKADVLALLGRYRSWPAFRRTVQLVLRYLRDRI
CCEEHEHHHHHHCCCHHHHHHHCCHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
SYARGTRLVMGNALVGRMLFRLRHSGVEIRFGTRLLSIEKSGNRVSGIVVERNGVTSSIS
HHHCCCEEEECHHHHHHHHHHHHHCCEEEEECCEEEEEECCCCEEEEEEEECCCCCHHHH
ARLGVVLATGGIGHNEKLRRDLPTGAVESLAPPSVRGDALAAAQDVGAIIERQARDFLWQ
HCEEEEEEECCCCCCHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
PVSRVPDGNGGWRLFPHLYLDRAKPGLIAVNSAGQRFVNEGASYHHFAECMMGSGKTLDE
HHHHCCCCCCCEEECHHHHHHCCCCCEEEECCCHHHHHHCCCCHHHHHHHHCCCCCCCCC
NLPAYLVCDHQFIQKYGIGVVPPGCRNLSPFKRSGYLVSAGSISELAQKIGVSPFGLSAT
CCCEEEEECHHHHHHCCCCCCCCCCCCCCCHHHCCEEEECCCHHHHHHHHCCCCCCHHHH
LQQYNADALDGKDSAFGKGETSVSRFNGDASKHPNPCLGPVQAPPFYAVQVFPADAASCT
HHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCC
GLRTDKDARVLDDTETSIDGLYACGNDMASVLKGAYPGPGATLGPAIVFAYRAALHAQRK
CCCCCCCCCEECCCCCCHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
TLTSSQKEARVYEEQ
HHHCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]