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The map label for this gene is pobB [H]

Identifier: 94314468

GI number: 94314468

Start: 2296516

End: 2297481

Strand: Direct

Name: pobB [H]

Synonym: Rmet_5549

Alternate gene names: 94314468

Gene position: 2296516-2297481 (Clockwise)

Preceding gene: 94314467

Following gene: 94314469

Centisome position: 89.01

GC content: 58.9

Gene sequence:

>966_bases
ATGAGCATGAACACCTCGCAAATTGAACTTCGACTGAAGCGAATCACGTTCGAAGCAGAGCGCATCTCTTCGTTCGAATT
TGTTTCCGCTGATGCGAATCCCTTACCGGGCTTCCAACCCGGCGCACACATCGACCTGCATCTCCAACAGGGAATGATCC
GCAGCTATTCCCTTGCCAATGCAGCGTCGGCAGATCCTTTGTATTACCGTGTCGCCGTCCAGCGCGAACCCAATGGCCGA
GGTGGCTCCCGCTGGGCCCACGACAAACTCCGCGTGGGTGACAAGGTCTGGGCCACGCCTCCACAGAACGACTTCCCCCT
TGACGAATGTGCGTCGAGCACCGTTTTCTTCGTGGGCGGCATCGGAATTACCCCTGTCCTTCCGATGCTCCGCAGACTCG
ACGCCATCGGCCGAGAATGGAAGTTGATGTACGCCAGCCGTTCACCGGGCGAAACCGCCTTCGCCGAAGAGCTCCAGCGC
ATCGATGCCGGGCGAGGCCGGATTGTCCATTTTCATGATTCCGAGCCTGGTCAGCGTCTGGAGATCCGTGCCACGGTTGC
AGCACTGCCCGCTTCAACGCATGTGTACTGCTGCGGCCCCACCGGCATGATTGACGACTTCCTGGCAGTCACCGCCGAGC
GAGACCCGTCCACAGTTCACTACGAACGCTTCGGTGCAGCCCAACAGGCGGCAACGGAGGGCGGCTTTGATGTGGTCTTG
CATAGGAGTGGCGCAAAGTACCGCGTGGAGCCCGGCAAGACCATCCTGGATGTGCTGTTAGATAATAACGTTGCAGTGCC
CTATTCCTGTTGCAACGGCGTCTGCGGAAGCTGCCGGACGGAGATCATCGATGGTCAAGCCGACCATCGCGATGACTTCC
TCAGTGACGAAGAGAAGCAGGCCAACCAGGCCATCATGGTTTGCTGCTCTGGCGCACGCTCCAAGACGCTAGTGCTAGAC
CTTTGA

Upstream 100 bases:

>100_bases
CATCCCACTTGGCGGCGACTGGACGAAGCTAGGCGAAGAAGCAATGCGCGCCGAACCGGGCAAGGACTTTGGCTACACGC
CCTGATACGTAGTACTGGAA

Downstream 100 bases:

>100_bases
TCGGCGGGACAGGGCAACAGAACGCCAATGGAAAGCAAATCATGACCTACGCACAAGAAGCCACCGCTGAACCGCGCGCC
AGCGCCGGCACGAACGTTAT

Product: Phenoxybenzoate dioxygenase subunit beta; phthalate 4,5-dioxygenase subunit beta

Products: NA

Alternate protein names: 4-carboxydiphenyl ether; phenoxybenzoate dioxygenase subunit beta [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSMNTSQIELRLKRITFEAERISSFEFVSADANPLPGFQPGAHIDLHLQQGMIRSYSLANAASADPLYYRVAVQREPNGR
GGSRWAHDKLRVGDKVWATPPQNDFPLDECASSTVFFVGGIGITPVLPMLRRLDAIGREWKLMYASRSPGETAFAEELQR
IDAGRGRIVHFHDSEPGQRLEIRATVAALPASTHVYCCGPTGMIDDFLAVTAERDPSTVHYERFGAAQQAATEGGFDVVL
HRSGAKYRVEPGKTILDVLLDNNVAVPYSCCNGVCGSCRTEIIDGQADHRDDFLSDEEKQANQAIMVCCSGARSKTLVLD
L

Sequences:

>Translated_321_residues
MSMNTSQIELRLKRITFEAERISSFEFVSADANPLPGFQPGAHIDLHLQQGMIRSYSLANAASADPLYYRVAVQREPNGR
GGSRWAHDKLRVGDKVWATPPQNDFPLDECASSTVFFVGGIGITPVLPMLRRLDAIGREWKLMYASRSPGETAFAEELQR
IDAGRGRIVHFHDSEPGQRLEIRATVAALPASTHVYCCGPTGMIDDFLAVTAERDPSTVHYERFGAAQQAATEGGFDVVL
HRSGAKYRVEPGKTILDVLLDNNVAVPYSCCNGVCGSCRTEIIDGQADHRDDFLSDEEKQANQAIMVCCSGARSKTLVLD
L
>Mature_320_residues
SMNTSQIELRLKRITFEAERISSFEFVSADANPLPGFQPGAHIDLHLQQGMIRSYSLANAASADPLYYRVAVQREPNGRG
GSRWAHDKLRVGDKVWATPPQNDFPLDECASSTVFFVGGIGITPVLPMLRRLDAIGREWKLMYASRSPGETAFAEELQRI
DAGRGRIVHFHDSEPGQRLEIRATVAALPASTHVYCCGPTGMIDDFLAVTAERDPSTVHYERFGAAQQAATEGGFDVVLH
RSGAKYRVEPGKTILDVLLDNNVAVPYSCCNGVCGSCRTEIIDGQADHRDDFLSDEEKQANQAIMVCCSGARSKTLVLDL

Specific function: Degrades exclusively diarylether compounds having carboxyl groups in the 3- or 4-position. Yields a hemiacetal that spontaneously hydrolyzes to phenol and protocatechuate [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=320, Percent_Identity=38.4375, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI1787098, Length=316, Percent_Identity=24.0506329113924, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787658, Length=327, Percent_Identity=25.3822629969419, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788903, Length=245, Percent_Identity=27.3469387755102, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 35217; Mature: 35086

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMNTSQIELRLKRITFEAERISSFEFVSADANPLPGFQPGAHIDLHLQQGMIRSYSLAN
CCCCCHHHEEEEEEEEEEHHHCCCEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHCC
AASADPLYYRVAVQREPNGRGGSRWAHDKLRVGDKVWATPPQNDFPLDECASSTVFFVGG
CCCCCCEEEEEEEEECCCCCCCCCCCCCHHHCCCEEECCCCCCCCCHHHHCCCCEEEEEC
IGITPVLPMLRRLDAIGREWKLMYASRSPGETAFAEELQRIDAGRGRIVHFHDSEPGQRL
CCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCEE
EIRATVAALPASTHVYCCGPTGMIDDFLAVTAERDPSTVHYERFGAAQQAATEGGFDVVL
EEEEEEEECCCCCEEEEECCCCHHHHHHHEECCCCCCEEEHHHHCCHHHHHCCCCEEEEE
HRSGAKYRVEPGKTILDVLLDNNVAVPYSCCNGVCGSCRTEIIDGQADHRDDFLSDEEKQ
ECCCCEEEECCCHHEEHHHHCCCCCCCHHHHCCCCCCCHHHHCCCCCCCCCCCCCCHHHH
ANQAIMVCCSGARSKTLVLDL
CCCEEEEEECCCCCCEEEEEC
>Mature Secondary Structure 
SMNTSQIELRLKRITFEAERISSFEFVSADANPLPGFQPGAHIDLHLQQGMIRSYSLAN
CCCCHHHEEEEEEEEEEHHHCCCEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHCC
AASADPLYYRVAVQREPNGRGGSRWAHDKLRVGDKVWATPPQNDFPLDECASSTVFFVGG
CCCCCCEEEEEEEEECCCCCCCCCCCCCHHHCCCEEECCCCCCCCCHHHHCCCCEEEEEC
IGITPVLPMLRRLDAIGREWKLMYASRSPGETAFAEELQRIDAGRGRIVHFHDSEPGQRL
CCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCEE
EIRATVAALPASTHVYCCGPTGMIDDFLAVTAERDPSTVHYERFGAAQQAATEGGFDVVL
EEEEEEEECCCCCEEEEECCCCHHHHHHHEECCCCCCEEEHHHHCCHHHHHCCCCEEEEE
HRSGAKYRVEPGKTILDVLLDNNVAVPYSCCNGVCGSCRTEIIDGQADHRDDFLSDEEKQ
ECCCCEEEECCCHHEEHHHHCCCCCCCHHHHCCCCCCCHHHHCCCCCCCCCCCCCCHHHH
ANQAIMVCCSGARSKTLVLDL
CCCEEEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7710319 [H]