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The map label for this gene is lutR [H]

Identifier: 94314469

GI number: 94314469

Start: 2297523

End: 2298245

Strand: Direct

Name: lutR [H]

Synonym: Rmet_5550

Alternate gene names: 94314469

Gene position: 2297523-2298245 (Clockwise)

Preceding gene: 94314468

Following gene: 94314470

Centisome position: 89.05

GC content: 60.86

Gene sequence:

>723_bases
ATGACCTACGCACAAGAAGCCACCGCTGAACCGCGCGCCAGCGCCGGCACGAACGTTATTGCCGGCATCCTGGCGTATTT
GCAGGACCGGCGCCTGCAACCAGGCGATCGCTTGCCATCTGAGCGCGACCTGGCCGAGCGACTCGGTGTTGGCCGAAATG
CTGTTCGTGAAGCGCTTGCGACTCTTGTCACACTTCGGGTGGTCGAGTCGCGCCCCAACTCCGGCGTCTATCTGCGTCAC
ATGTCCCGGGAAAGCAGCTTCGAAGCGCTCGTACTGCTGACCGACATTGGCGCAACGCCCACCCCGACTGAGGTGACGGA
GACGATGGAGGTTCGTGCCCATCTTGAGTTGCTCGCCGTCAGCCTGGCGTGCCAACGCCGCACCGACGAAGACCTCGCCC
GGATGGAAGCGATCCTTGCCCGCACTGACCAAACGCTCGAGGCTGGCGGAAACATCGCCGAGATGGATACGGATTTCCAT
ATCGCCGTCGTCGACGCCGCACACAACTCAGTGCTGGTTCGCACCCTCAACGCCTTCTATCGGTTTACCGCACGCCGACG
AGAAGTACTGTTTGCTGACCATACCCAAGGCCTCGCGTCGGCCCGCGAGCATCGCCAAATGCTGGAGCACATCAGCAACC
GCGACATCTCAAAAGCTGAACGCTTAATCCTCCAGCATATGGATCGAGCAACCACCTACTGGTCCAGCTACCTGGAAACC
TGA

Upstream 100 bases:

>100_bases
AGGCCATCATGGTTTGCTGCTCTGGCGCACGCTCCAAGACGCTAGTGCTAGACCTTTGATCGGCGGGACAGGGCAACAGA
ACGCCAATGGAAAGCAAATC

Downstream 100 bases:

>100_bases
ACCTAGCGGCCACCGAGCCGCGACCCACAACTAGGAGACAAAACCATGCGCCCCCGGTCTTTGAACCTGCGTGCGATGCT
CTCGCTCGCCCTTTTTTTGG

Product: putative GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MTYAQEATAEPRASAGTNVIAGILAYLQDRRLQPGDRLPSERDLAERLGVGRNAVREALATLVTLRVVESRPNSGVYLRH
MSRESSFEALVLLTDIGATPTPTEVTETMEVRAHLELLAVSLACQRRTDEDLARMEAILARTDQTLEAGGNIAEMDTDFH
IAVVDAAHNSVLVRTLNAFYRFTARRREVLFADHTQGLASAREHRQMLEHISNRDISKAERLILQHMDRATTYWSSYLET

Sequences:

>Translated_240_residues
MTYAQEATAEPRASAGTNVIAGILAYLQDRRLQPGDRLPSERDLAERLGVGRNAVREALATLVTLRVVESRPNSGVYLRH
MSRESSFEALVLLTDIGATPTPTEVTETMEVRAHLELLAVSLACQRRTDEDLARMEAILARTDQTLEAGGNIAEMDTDFH
IAVVDAAHNSVLVRTLNAFYRFTARRREVLFADHTQGLASAREHRQMLEHISNRDISKAERLILQHMDRATTYWSSYLET
>Mature_239_residues
TYAQEATAEPRASAGTNVIAGILAYLQDRRLQPGDRLPSERDLAERLGVGRNAVREALATLVTLRVVESRPNSGVYLRHM
SRESSFEALVLLTDIGATPTPTEVTETMEVRAHLELLAVSLACQRRTDEDLARMEAILARTDQTLEAGGNIAEMDTDFHI
AVVDAAHNSVLVRTLNAFYRFTARRREVLFADHTQGLASAREHRQMLEHISNRDISKAERLILQHMDRATTYWSSYLET

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790032, Length=159, Percent_Identity=32.0754716981132, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790780, Length=206, Percent_Identity=29.6116504854369, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786303, Length=180, Percent_Identity=31.6666666666667, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI48994955, Length=194, Percent_Identity=25.2577319587629, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789621, Length=215, Percent_Identity=26.046511627907, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 26887; Mature: 26756

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYAQEATAEPRASAGTNVIAGILAYLQDRRLQPGDRLPSERDLAERLGVGRNAVREALA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHH
TLVTLRVVESRPNSGVYLRHMSRESSFEALVLLTDIGATPTPTEVTETMEVRAHLELLAV
HHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
SLACQRRTDEDLARMEAILARTDQTLEAGGNIAEMDTDFHIAVVDAAHNSVLVRTLNAFY
HHHHHCCCHHHHHHHHHHHHHCCHHHHCCCCEEEECCCEEEEEEECCCCHHHHHHHHHHH
RFTARRREVLFADHTQGLASAREHRQMLEHISNRDISKAERLILQHMDRATTYWSSYLET
HHHHHHHHHEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TYAQEATAEPRASAGTNVIAGILAYLQDRRLQPGDRLPSERDLAERLGVGRNAVREALA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHH
TLVTLRVVESRPNSGVYLRHMSRESSFEALVLLTDIGATPTPTEVTETMEVRAHLELLAV
HHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
SLACQRRTDEDLARMEAILARTDQTLEAGGNIAEMDTDFHIAVVDAAHNSVLVRTLNAFY
HHHHHCCCHHHHHHHHHHHHHCCHHHHCCCCEEEECCCEEEEEEECCCCHHHHHHHHHHH
RFTARRREVLFADHTQGLASAREHRQMLEHISNRDISKAERLILQHMDRATTYWSSYLET
HHHHHHHHHEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]