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The map label for this gene is gadW [C]

Identifier: 94314432

GI number: 94314432

Start: 2258985

End: 2260031

Strand: Direct

Name: gadW [C]

Synonym: Rmet_5513

Alternate gene names: 94314432

Gene position: 2258985-2260031 (Clockwise)

Preceding gene: 94314431

Following gene: 94314444

Centisome position: 87.55

GC content: 57.69

Gene sequence:

>1047_bases
ATGAGTAACGCTGAGAGTTTGTCAAAAAGCGCAGAGACGCCGAGAGACGGCATCCCACTGTGCTACTTGCAGTTGCTGCT
ATTGCCGGCCCGAGCAAAGGGCTACGACACGGACGCGTTGTTGCGGCACCATGGGCTTTTGTCTGCTCTAGACAGTTCGC
CAAATCAAATCGTCACGATGCTGCAGTTCGCGCGAATTCTGCGGCGCTTGCGGAGACTGCTCCATGACGAGATGATTGCC
GTCACCGACCGCCCAGTTCGTCCCGGTACGTTCTTGCTCGTCGTCCGCCAGATGCTGCAATGTACAACGCTGGGAGAAGC
GCTTCGACTTGGTTGCAGCCTCTATCGACTTGTCATCGAGGACTTCTCACCCCGTCTTCGCATATATGGCGATGTTGCTC
GCCTGGAGATAGTCGACGCTTCACCACCAGGTACATTCCGAAGCATCGCACACCTCATGATGCTATACGGCGCCATCGGG
CTCATGTCCTGGATGGTCCAGCGACCGATTGCCGTGCATGAAGTCACGCTTCCAGCGTCATATCCATCCCTCGCCCCCGC
GGACGCCTTGTTCCAAGCGCCCGTACGCGCTGCCTCCATTAGTGGAATCAGCTTCGAATCGAGCCACCTCAACGAACGAG
TTGTGACAGACATCGGGGGATTGAGAACGTTCCTGCTTCATTGGCCGATCCGAAAGATGGCACCTTACAGCGAGAAACTT
CCTCTAGCTGTCCAGGTTAGAAAACGCCTTATTCAACGGGATATCGCGCATCTCCCTGCCCAAGCGGAGCTTGCTGCTAC
GATGGGGCTGACCGACAAGGCGTTGCGCCGTCGACTGTTCCAGGAAGGACAGAGCTACCGAGCCATCGTCGACGCGCTTC
GGCGTGATGCCGCGATACGGCTTCTCGAGCAGTCCAGACTCAGCGTTGCCGAAATTGGGATCCGCCTGGGATTCTCAGAG
CCCAGCGCCTTTCACCGCGCTTTTCGCCGAGCAACAGGCCTGACGCCGAATCAATTTCGGCGCCAAGCTTCGGTGGACCC
CAACTAG

Upstream 100 bases:

>100_bases
GGCGAGCCTCCGCAAATTTCGCAGACCTCCCAGTGCGCACGCAGTTCCAAAAGTCGGAATAGAAGCCGCATTACGGCATG
AAGTAGAGGCACTGACCGAC

Downstream 100 bases:

>100_bases
GCAGCGCTGTAGCCTCCGTCGACAGTAAGCTCAGTGCCAGTGACGAATCGAGACTCGTCTGCACAAAGGAACGCGATGAC
ATTCGCCACGTCAACGGGTT

Product: putative AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MSNAESLSKSAETPRDGIPLCYLQLLLLPARAKGYDTDALLRHHGLLSALDSSPNQIVTMLQFARILRRLRRLLHDEMIA
VTDRPVRPGTFLLVVRQMLQCTTLGEALRLGCSLYRLVIEDFSPRLRIYGDVARLEIVDASPPGTFRSIAHLMMLYGAIG
LMSWMVQRPIAVHEVTLPASYPSLAPADALFQAPVRAASISGISFESSHLNERVVTDIGGLRTFLLHWPIRKMAPYSEKL
PLAVQVRKRLIQRDIAHLPAQAELAATMGLTDKALRRRLFQEGQSYRAIVDALRRDAAIRLLEQSRLSVAEIGIRLGFSE
PSAFHRAFRRATGLTPNQFRRQASVDPN

Sequences:

>Translated_348_residues
MSNAESLSKSAETPRDGIPLCYLQLLLLPARAKGYDTDALLRHHGLLSALDSSPNQIVTMLQFARILRRLRRLLHDEMIA
VTDRPVRPGTFLLVVRQMLQCTTLGEALRLGCSLYRLVIEDFSPRLRIYGDVARLEIVDASPPGTFRSIAHLMMLYGAIG
LMSWMVQRPIAVHEVTLPASYPSLAPADALFQAPVRAASISGISFESSHLNERVVTDIGGLRTFLLHWPIRKMAPYSEKL
PLAVQVRKRLIQRDIAHLPAQAELAATMGLTDKALRRRLFQEGQSYRAIVDALRRDAAIRLLEQSRLSVAEIGIRLGFSE
PSAFHRAFRRATGLTPNQFRRQASVDPN
>Mature_347_residues
SNAESLSKSAETPRDGIPLCYLQLLLLPARAKGYDTDALLRHHGLLSALDSSPNQIVTMLQFARILRRLRRLLHDEMIAV
TDRPVRPGTFLLVVRQMLQCTTLGEALRLGCSLYRLVIEDFSPRLRIYGDVARLEIVDASPPGTFRSIAHLMMLYGAIGL
MSWMVQRPIAVHEVTLPASYPSLAPADALFQAPVRAASISGISFESSHLNERVVTDIGGLRTFLLHWPIRKMAPYSEKLP
LAVQVRKRLIQRDIAHLPAQAELAATMGLTDKALRRRLFQEGQSYRAIVDALRRDAAIRLLEQSRLSVAEIGIRLGFSEP
SAFHRAFRRATGLTPNQFRRQASVDPN

Specific function: Depending On The Conditions (Growth Phase And Medium), Acts As A Positive Or Negative Regulator Of Gada And Gadbc. Repression Occurs Directly Or Via The Repression Of The Expression Of Gadx. Activation Occurs Directly By The Binding Of Gadw To The Gada An

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 38915; Mature: 38784

Theoretical pI: Translated: 10.75; Mature: 10.75

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNAESLSKSAETPRDGIPLCYLQLLLLPARAKGYDTDALLRHHGLLSALDSSPNQIVTM
CCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
LQFARILRRLRRLLHDEMIAVTDRPVRPGTFLLVVRQMLQCTTLGEALRLGCSLYRLVIE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFSPRLRIYGDVARLEIVDASPPGTFRSIAHLMMLYGAIGLMSWMVQRPIAVHEVTLPAS
CCCCCEEEEECEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCC
YPSLAPADALFQAPVRAASISGISFESSHLNERVVTDIGGLRTFLLHWPIRKMAPYSEKL
CCCCCCHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCC
PLAVQVRKRLIQRDIAHLPAQAELAATMGLTDKALRRRLFQEGQSYRAIVDALRRDAAIR
CHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LLEQSRLSVAEIGIRLGFSEPSAFHRAFRRATGLTPNQFRRQASVDPN
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
SNAESLSKSAETPRDGIPLCYLQLLLLPARAKGYDTDALLRHHGLLSALDSSPNQIVTM
CCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
LQFARILRRLRRLLHDEMIAVTDRPVRPGTFLLVVRQMLQCTTLGEALRLGCSLYRLVIE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFSPRLRIYGDVARLEIVDASPPGTFRSIAHLMMLYGAIGLMSWMVQRPIAVHEVTLPAS
CCCCCEEEEECEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCC
YPSLAPADALFQAPVRAASISGISFESSHLNERVVTDIGGLRTFLLHWPIRKMAPYSEKL
CCCCCCHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCC
PLAVQVRKRLIQRDIAHLPAQAELAATMGLTDKALRRRLFQEGQSYRAIVDALRRDAAIR
CHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LLEQSRLSVAEIGIRLGFSEPSAFHRAFRRATGLTPNQFRRQASVDPN
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036; 12368430 [H]