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The map label for this gene is tnsA [H]

Identifier: 94314378

GI number: 94314378

Start: 2206782

End: 2207735

Strand: Reverse

Name: tnsA [H]

Synonym: Rmet_5459

Alternate gene names: 94314378

Gene position: 2207735-2206782 (Counterclockwise)

Preceding gene: 94314379

Following gene: 94314377

Centisome position: 85.57

GC content: 58.07

Gene sequence:

>954_bases
GTGCACGCCACAAAAGCTAGTCCCGGCTTTGAACAATTATCGGAGTTGGACTTGACCAAAAAGTCACAAGATTTGTCGGC
GGTAGCCAAGCGTCTTCTCAATCCCGCAGTGTCGGGCCGCGCGAAGGGGAAAGAAGTCATACGTACGCGGAAGGCCGGCG
TATGGTCTCCGTTTGAAATGAATCCACAGAAATTAGAAAGATTTCTGCGTGAAGGGCGAGGAATGGGAAGCGGGAACGCG
TACCGTCCGTGGATTCAGGTCAACGACTTCTCGTCCCTGGGCCGCGTCCATCGCACGTCGTCGGCACTCACGGGCTATCG
AACACACCATCTACTGTCTGACAACGAGTACTACGCCTACCTGGAAGCATGGTGGGATGAGGAGATGCTGGACATTCGTG
AACAGTATCCGCTGCTCCCGGTCGCGCTCACCTGCGGCATTGCCGCCGAGTTGGGCTTCAGGCATCCCTACAGCACGAAG
TACCGACTCGCCATTCCCCTTACAACGGACCTGCTGCTCGTCAGGAAGCGCGGACTCGAAGCCGTCGCAGTAGTGGAAGA
CGCCCACTACGAAGAACAACGTACGGAAGAAAAGCTGGCGATCGCCGAGGAATTTTGGCGGCGACGGGGTGTTCCGACGC
GGGTTGCGCTTTCCTCAACGCTGAAGGTGCAGCGTTCCCGAAACCTGCATTGGATTTATCAAGCGCGTCGCGCGCCCACC
GAGCGTCCGTATGCGCCTGGTGCGAATCCTCTGCATGATTGCCTGGTAGGCCAAGTCGAGGGCGGACCTATGAGTTTCTG
GGAGCTGGCCTTCCGCGCGGACCTCGCGCTTTGCCTTCCCATGGGGACATCCATCGCTGCGATTCGCTGGCTGCTGGCCG
AGCGTGTCCTCTCGACCGATATCGACGACGGCGACATCACTAAGGATTGTGAGATTTTCGTTCGGAGCAAGTGA

Upstream 100 bases:

>100_bases
CCCGAAATATTCCCTCCCAGCGTTGGACTTTTCGTCATCCGCATCCAAAGCGCACGAGCCCGGCATTTTTTCGGCCTATA
CGCTCGCACGGGACTTTAAT

Downstream 100 bases:

>100_bases
TGAGCAATCTCCAAATCCAACGTCTGCCTGAGCTGGCCACCAACATGTTGTTCCGGCTGCGGAATGACCCGAAGAGTCCG
CTCCAGCGCATCGTTTGGAC

Product: transposase Tn7-like proteinA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MHATKASPGFEQLSELDLTKKSQDLSAVAKRLLNPAVSGRAKGKEVIRTRKAGVWSPFEMNPQKLERFLREGRGMGSGNA
YRPWIQVNDFSSLGRVHRTSSALTGYRTHHLLSDNEYYAYLEAWWDEEMLDIREQYPLLPVALTCGIAAELGFRHPYSTK
YRLAIPLTTDLLLVRKRGLEAVAVVEDAHYEEQRTEEKLAIAEEFWRRRGVPTRVALSSTLKVQRSRNLHWIYQARRAPT
ERPYAPGANPLHDCLVGQVEGGPMSFWELAFRADLALCLPMGTSIAAIRWLLAERVLSTDIDDGDITKDCEIFVRSK

Sequences:

>Translated_317_residues
MHATKASPGFEQLSELDLTKKSQDLSAVAKRLLNPAVSGRAKGKEVIRTRKAGVWSPFEMNPQKLERFLREGRGMGSGNA
YRPWIQVNDFSSLGRVHRTSSALTGYRTHHLLSDNEYYAYLEAWWDEEMLDIREQYPLLPVALTCGIAAELGFRHPYSTK
YRLAIPLTTDLLLVRKRGLEAVAVVEDAHYEEQRTEEKLAIAEEFWRRRGVPTRVALSSTLKVQRSRNLHWIYQARRAPT
ERPYAPGANPLHDCLVGQVEGGPMSFWELAFRADLALCLPMGTSIAAIRWLLAERVLSTDIDDGDITKDCEIFVRSK
>Mature_317_residues
MHATKASPGFEQLSELDLTKKSQDLSAVAKRLLNPAVSGRAKGKEVIRTRKAGVWSPFEMNPQKLERFLREGRGMGSGNA
YRPWIQVNDFSSLGRVHRTSSALTGYRTHHLLSDNEYYAYLEAWWDEEMLDIREQYPLLPVALTCGIAAELGFRHPYSTK
YRLAIPLTTDLLLVRKRGLEAVAVVEDAHYEEQRTEEKLAIAEEFWRRRGVPTRVALSSTLKVQRSRNLHWIYQARRAPT
ERPYAPGANPLHDCLVGQVEGGPMSFWELAFRADLALCLPMGTSIAAIRWLLAERVLSTDIDDGDITKDCEIFVRSK

Specific function: Required for Tn7 transposition. Forms the transposase, together with TnsB. TnsA executes the 5'-DNA strand breakage reaction. TnsABC and tnsD promote high-frequency insertion of Tn7 into a specific target site known as ATT-Tn7 whereas tnsABC and tnsD prom

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011856
- InterPro:   IPR011335
- InterPro:   IPR014832
- InterPro:   IPR014833
- InterPro:   IPR011991 [H]

Pfam domain/function: PF08721 TnsA_C; PF08722 TnsA_N [H]

EC number: NA

Molecular weight: Translated: 35999; Mature: 35999

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHATKASPGFEQLSELDLTKKSQDLSAVAKRLLNPAVSGRAKGKEVIRTRKAGVWSPFEM
CCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHCCCCCCCCC
NPQKLERFLREGRGMGSGNAYRPWIQVNDFSSLGRVHRTSSALTGYRTHHLLSDNEYYAY
CHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHEEECCCCEEEE
LEAWWDEEMLDIREQYPLLPVALTCGIAAELGFRHPYSTKYRLAIPLTTDLLLVRKRGLE
EEHHCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHCCCC
AVAVVEDAHYEEQRTEEKLAIAEEFWRRRGVPTRVALSSTLKVQRSRNLHWIYQARRAPT
EEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCEEEEEHHHCCCC
ERPYAPGANPLHDCLVGQVEGGPMSFWELAFRADLALCLPMGTSIAAIRWLLAERVLSTD
CCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHCC
IDDGDITKDCEIFVRSK
CCCCCCCCCEEEEEECC
>Mature Secondary Structure
MHATKASPGFEQLSELDLTKKSQDLSAVAKRLLNPAVSGRAKGKEVIRTRKAGVWSPFEM
CCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHCCCCCCCCC
NPQKLERFLREGRGMGSGNAYRPWIQVNDFSSLGRVHRTSSALTGYRTHHLLSDNEYYAY
CHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHEEECCCCEEEE
LEAWWDEEMLDIREQYPLLPVALTCGIAAELGFRHPYSTKYRLAIPLTTDLLLVRKRGLE
EEHHCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHCCCC
AVAVVEDAHYEEQRTEEKLAIAEEFWRRRGVPTRVALSSTLKVQRSRNLHWIYQARRAPT
EEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCEEEEEHHHCCCC
ERPYAPGANPLHDCLVGQVEGGPMSFWELAFRADLALCLPMGTSIAAIRWLLAERVLSTD
CCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHCC
IDDGDITKDCEIFVRSK
CCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2156235; 1655576; 3010949; 8947057; 10704304 [H]