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The map label for this gene is tnsB [H]

Identifier: 94314377

GI number: 94314377

Start: 2204605

End: 2206782

Strand: Reverse

Name: tnsB [H]

Synonym: Rmet_5458

Alternate gene names: 94314377

Gene position: 2206782-2204605 (Counterclockwise)

Preceding gene: 94314378

Following gene: 94314375

Centisome position: 85.53

GC content: 57.16

Gene sequence:

>2178_bases
ATGAGCAATCTCCAAATCCAACGTCTGCCTGAGCTGGCCACCAACATGTTGTTCCGGCTGCGGAATGACCCGAAGAGTCC
GCTCCAGCGCATCGTTTGGACAGGTGGCCAGTCGTACCACACCTTCTATGTGCCGGTAAGCGGTGATGATTGGCCGCAAG
CATGCCTCACGGACGAACTGAGGGCACGGTTCAGTCCTGACTACGTTGGCGACGACGCTCTGGTCTTCGTTGGAGAGGAC
CCGTACGCAGTCAAGGTCCGGGCTGAGCCTAGTGCCTTTGCCCTTGAACGAATGCGCTGGATTTTCGATTTGGTTAAGGG
AAAGAAGCTGATCGACATTCTGACCCCGAGTAAACGCGGCAAGGCTATCGAGGAGCAGGTGGCCAAAGTGGGGAAGCCCC
GGGCTACGCTCACCTTTCAGCTCAAGCGATACCTTGCCCGTGGAATGACGGTCTCCGCTCTGGAGGGCGATCTGGCCAAT
TGCGGCGGCCGCGGCAAGCAAAGGAATGCACTGAGTAAGGTCGGGGCAAAGAGGAGCGTGAAGGCAGGCGATGGCACACA
AGTTAACGAATCCAACCGCTCCATACTTGAAGCCGGCGTAAGTTACTTGCTCGGTGCGCCGAAGCGCACGCTGCAACTGG
CTCTCGACTTTATCCACGAGAAGTTCTGTCACGACTGGCCCGCGACAAAGCTCTTCACCAGACGCCAGCTTCAGCATCAC
CTGTACTTCACGCGCCCGTTTACCCAGCGCAAACGCAAGAAGCATGGTGAACGGAGATTCAATCTTTCGATGCGCGCGTT
CAACGGGACGGTGGATACGTACGGGCCGGGTGCGCAGTATCAGATAGACGCGACGATTGCCGACGTGTATCTCGTGTCGG
CTCTGGACAGGCTTTCGATTGTCGGTCGGCCGACGATCTACATCGTCTCAGATACGTTCTCTCGGCAGATTGTTGGTGTG
TACGTCACTCTCTCCCCTCCTTCAATTGAGGGGGCCGCGCTGGCGATGGAGAGCGTTGTGACGCCGAAGGTTGCCCTTTG
CGCCGAATACGGAATCGAAACCAAAGAGGAACACTGGCCTGCGCACCATTTATCGAGTCAGCTACTTGGAGACCGCGGCT
CGGAATTCATCAGCGTCAAGGCGTGGGATCGCATCGTTCAGCGAATGATTATGGACGTCGCTAATACGGCGGCGTTCCGG
CCCGACTGGAAGTCGATTGTCGAGTCGCGCTTCAATCTGCTCAACGCCGTTTGGGCGCCGTTTGTGCCGGGCTACGTCGA
CAAGGACTTTAGAGAGCGGGGCGGCAAGGATTATCGACTGGACGCGGTGCTCACCCTCCGCGAGTTCACAGCGGTGTTCC
TCGTCAGCGTCATCGAGTACAACAACCGCCCCCTCCGCACGAAGAAGCAGATTCCTGAGATGGTTGCCGCAAAGCTGAGT
CCAACCCCCGTGGAACTGTGGAAGTTCGGTATCAGCAAGCTCAGTGGAGCACTCCGGACTGTTCACATCGACGAGGTTCG
AGCCTGCGTGTACCCGCGCGGTGAAGCCGTAGTCTCTGAATATGGTATCGGGTTTGCGACTCGCTTCTATGAGACACCGC
GTGCGATGAAGGAAGAGTGGTTCCCCCGATCCCGAAAGAAGAACTTTAAGGTCGAGGTCGCATATGACCCGATTAATCCG
GCAAAGCTGTTCGTCCTCCATGAAGACGGATCGTTCGAGAGAGCGACGCTTCGGATGACGGCAGGGCAGCAGTATGACGA
GGTAGTGAGCCTGGCAGAAATCGAAAAGCTGCAGAAGGACGCTGCGCGCACTTTGAATGATGGCTTTGACAACGCGGAGC
CACTCAAGCGTGCACTGCGTGACCAGAGGGAAGCCATCGTCGAGGGAGCCATCAAGGAGAAGGAAGAGCGCATGGTCGCA
GCCGGCGTCAAGCAGCTAGATACGAAGAGTATCCGCGACGCTAAGGCAAATGAGCGCGAACTCGAAGACCTGCTACGCAG
CGGTCAGGTGCCTGAAATCCTGCGCAACGAACCTCCTGTCGAGACACCCAAGAAGGTGGAAGAGCAGGCCGAGGAGTCGT
GGGAAGACTTCGGCGATCTTTCAATCGCGCTCCTGGAAGCAGCAAAGAGAAACCCTCCAGATGACCTGAAAGGAGACAAA
GATGAAAACCAAGCCTAA

Upstream 100 bases:

>100_bases
TCGCTGCGATTCGCTGGCTGCTGGCCGAGCGTGTCCTCTCGACCGATATCGACGACGGCGACATCACTAAGGATTGTGAG
ATTTTCGTTCGGAGCAAGTG

Downstream 100 bases:

>100_bases
CGAATACATACTCCCTTCACATACGTTCGAGGGGGAGCGCGATTTCATCGAAGCGGAGTACCTGCCGTCGCTCATCCCCG
AGTTCGCAGGGAATCCGCTC

Product: Tn7-like transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 725; Mature: 724

Protein sequence:

>725_residues
MSNLQIQRLPELATNMLFRLRNDPKSPLQRIVWTGGQSYHTFYVPVSGDDWPQACLTDELRARFSPDYVGDDALVFVGED
PYAVKVRAEPSAFALERMRWIFDLVKGKKLIDILTPSKRGKAIEEQVAKVGKPRATLTFQLKRYLARGMTVSALEGDLAN
CGGRGKQRNALSKVGAKRSVKAGDGTQVNESNRSILEAGVSYLLGAPKRTLQLALDFIHEKFCHDWPATKLFTRRQLQHH
LYFTRPFTQRKRKKHGERRFNLSMRAFNGTVDTYGPGAQYQIDATIADVYLVSALDRLSIVGRPTIYIVSDTFSRQIVGV
YVTLSPPSIEGAALAMESVVTPKVALCAEYGIETKEEHWPAHHLSSQLLGDRGSEFISVKAWDRIVQRMIMDVANTAAFR
PDWKSIVESRFNLLNAVWAPFVPGYVDKDFRERGGKDYRLDAVLTLREFTAVFLVSVIEYNNRPLRTKKQIPEMVAAKLS
PTPVELWKFGISKLSGALRTVHIDEVRACVYPRGEAVVSEYGIGFATRFYETPRAMKEEWFPRSRKKNFKVEVAYDPINP
AKLFVLHEDGSFERATLRMTAGQQYDEVVSLAEIEKLQKDAARTLNDGFDNAEPLKRALRDQREAIVEGAIKEKEERMVA
AGVKQLDTKSIRDAKANERELEDLLRSGQVPEILRNEPPVETPKKVEEQAEESWEDFGDLSIALLEAAKRNPPDDLKGDK
DENQA

Sequences:

>Translated_725_residues
MSNLQIQRLPELATNMLFRLRNDPKSPLQRIVWTGGQSYHTFYVPVSGDDWPQACLTDELRARFSPDYVGDDALVFVGED
PYAVKVRAEPSAFALERMRWIFDLVKGKKLIDILTPSKRGKAIEEQVAKVGKPRATLTFQLKRYLARGMTVSALEGDLAN
CGGRGKQRNALSKVGAKRSVKAGDGTQVNESNRSILEAGVSYLLGAPKRTLQLALDFIHEKFCHDWPATKLFTRRQLQHH
LYFTRPFTQRKRKKHGERRFNLSMRAFNGTVDTYGPGAQYQIDATIADVYLVSALDRLSIVGRPTIYIVSDTFSRQIVGV
YVTLSPPSIEGAALAMESVVTPKVALCAEYGIETKEEHWPAHHLSSQLLGDRGSEFISVKAWDRIVQRMIMDVANTAAFR
PDWKSIVESRFNLLNAVWAPFVPGYVDKDFRERGGKDYRLDAVLTLREFTAVFLVSVIEYNNRPLRTKKQIPEMVAAKLS
PTPVELWKFGISKLSGALRTVHIDEVRACVYPRGEAVVSEYGIGFATRFYETPRAMKEEWFPRSRKKNFKVEVAYDPINP
AKLFVLHEDGSFERATLRMTAGQQYDEVVSLAEIEKLQKDAARTLNDGFDNAEPLKRALRDQREAIVEGAIKEKEERMVA
AGVKQLDTKSIRDAKANERELEDLLRSGQVPEILRNEPPVETPKKVEEQAEESWEDFGDLSIALLEAAKRNPPDDLKGDK
DENQA
>Mature_724_residues
SNLQIQRLPELATNMLFRLRNDPKSPLQRIVWTGGQSYHTFYVPVSGDDWPQACLTDELRARFSPDYVGDDALVFVGEDP
YAVKVRAEPSAFALERMRWIFDLVKGKKLIDILTPSKRGKAIEEQVAKVGKPRATLTFQLKRYLARGMTVSALEGDLANC
GGRGKQRNALSKVGAKRSVKAGDGTQVNESNRSILEAGVSYLLGAPKRTLQLALDFIHEKFCHDWPATKLFTRRQLQHHL
YFTRPFTQRKRKKHGERRFNLSMRAFNGTVDTYGPGAQYQIDATIADVYLVSALDRLSIVGRPTIYIVSDTFSRQIVGVY
VTLSPPSIEGAALAMESVVTPKVALCAEYGIETKEEHWPAHHLSSQLLGDRGSEFISVKAWDRIVQRMIMDVANTAAFRP
DWKSIVESRFNLLNAVWAPFVPGYVDKDFRERGGKDYRLDAVLTLREFTAVFLVSVIEYNNRPLRTKKQIPEMVAAKLSP
TPVELWKFGISKLSGALRTVHIDEVRACVYPRGEAVVSEYGIGFATRFYETPRAMKEEWFPRSRKKNFKVEVAYDPINPA
KLFVLHEDGSFERATLRMTAGQQYDEVVSLAEIEKLQKDAARTLNDGFDNAEPLKRALRDQREAIVEGAIKEKEERMVAA
GVKQLDTKSIRDAKANERELEDLLRSGQVPEILRNEPPVETPKKVEEQAEESWEDFGDLSIALLEAAKRNPPDDLKGDKD
ENQA

Specific function: Sequence-specific, DNA-binding protein required for Tn7 transposition. Recognizes sequences necessary for recombination at both left and right ends of Tn7 and, together with tnsA, forms the transposase. TnsB executes the 5'-DNA strand breakage and joining

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 81953; Mature: 81822

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLQIQRLPELATNMLFRLRNDPKSPLQRIVWTGGQSYHTFYVPVSGDDWPQACLTDEL
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHH
RARFSPDYVGDDALVFVGEDPYAVKVRAEPSAFALERMRWIFDLVKGKKLIDILTPSKRG
HHCCCCCCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
KAIEEQVAKVGKPRATLTFQLKRYLARGMTVSALEGDLANCGGRGKQRNALSKVGAKRSV
CHHHHHHHHCCCCCCEEHHHHHHHHHCCCEEEHHCCCHHHCCCCCCHHHHHHHHCCCCCC
KAGDGTQVNESNRSILEAGVSYLLGAPKRTLQLALDFIHEKFCHDWPATKLFTRRQLQHH
CCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LYFTRPFTQRKRKKHGERRFNLSMRAFNGTVDTYGPGAQYQIDATIADVYLVSALDRLSI
EEECCCHHHHHHHHCCCHHEEEEEEEECCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHH
VGRPTIYIVSDTFSRQIVGVYVTLSPPSIEGAALAMESVVTPKVALCAEYGIETKEEHWP
CCCCEEEEEECCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCHHCCC
AHHLSSQLLGDRGSEFISVKAWDRIVQRMIMDVANTAAFRPDWKSIVESRFNLLNAVWAP
HHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
FVPGYVDKDFRERGGKDYRLDAVLTLREFTAVFLVSVIEYNNRPLRTKKQIPEMVAAKLS
CCCCCCCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
PTPVELWKFGISKLSGALRTVHIDEVRACVYPRGEAVVSEYGIGFATRFYETPRAMKEEW
CCHHHHHHHHHHHHHHHHHEEEHHHHHHHHCCCCHHHHHHHCCCHHHHHHHCHHHHHHHH
FPRSRKKNFKVEVAYDPINPAKLFVLHEDGSFERATLRMTAGQQYDEVVSLAEIEKLQKD
CCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
AARTLNDGFDNAEPLKRALRDQREAIVEGAIKEKEERMVAAGVKQLDTKSIRDAKANERE
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LEDLLRSGQVPEILRNEPPVETPKKVEEQAEESWEDFGDLSIALLEAAKRNPPDDLKGDK
HHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
DENQA
CCCCC
>Mature Secondary Structure 
SNLQIQRLPELATNMLFRLRNDPKSPLQRIVWTGGQSYHTFYVPVSGDDWPQACLTDEL
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHH
RARFSPDYVGDDALVFVGEDPYAVKVRAEPSAFALERMRWIFDLVKGKKLIDILTPSKRG
HHCCCCCCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
KAIEEQVAKVGKPRATLTFQLKRYLARGMTVSALEGDLANCGGRGKQRNALSKVGAKRSV
CHHHHHHHHCCCCCCEEHHHHHHHHHCCCEEEHHCCCHHHCCCCCCHHHHHHHHCCCCCC
KAGDGTQVNESNRSILEAGVSYLLGAPKRTLQLALDFIHEKFCHDWPATKLFTRRQLQHH
CCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LYFTRPFTQRKRKKHGERRFNLSMRAFNGTVDTYGPGAQYQIDATIADVYLVSALDRLSI
EEECCCHHHHHHHHCCCHHEEEEEEEECCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHH
VGRPTIYIVSDTFSRQIVGVYVTLSPPSIEGAALAMESVVTPKVALCAEYGIETKEEHWP
CCCCEEEEEECCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCHHCCC
AHHLSSQLLGDRGSEFISVKAWDRIVQRMIMDVANTAAFRPDWKSIVESRFNLLNAVWAP
HHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
FVPGYVDKDFRERGGKDYRLDAVLTLREFTAVFLVSVIEYNNRPLRTKKQIPEMVAAKLS
CCCCCCCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
PTPVELWKFGISKLSGALRTVHIDEVRACVYPRGEAVVSEYGIGFATRFYETPRAMKEEW
CCHHHHHHHHHHHHHHHHHEEEHHHHHHHHCCCCHHHHHHHCCCHHHHHHHCHHHHHHHH
FPRSRKKNFKVEVAYDPINPAKLFVLHEDGSFERATLRMTAGQQYDEVVSLAEIEKLQKD
CCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
AARTLNDGFDNAEPLKRALRDQREAIVEGAIKEKEERMVAAGVKQLDTKSIRDAKANERE
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LEDLLRSGQVPEILRNEPPVETPKKVEEQAEESWEDFGDLSIALLEAAKRNPPDDLKGDK
HHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
DENQA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2156235; 1655576; 1657979; 8947057; 10704304 [H]