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The map label for this gene is fadA [H]

Identifier: 94314351

GI number: 94314351

Start: 2178173

End: 2179351

Strand: Reverse

Name: fadA [H]

Synonym: Rmet_5432

Alternate gene names: 94314351

Gene position: 2179351-2178173 (Counterclockwise)

Preceding gene: 94314352

Following gene: 94314350

Centisome position: 84.47

GC content: 67.68

Gene sequence:

>1179_bases
ATGTCCGAAGCCTATATCGTTTCCGCCGTCCGCACCGCCGGCGGCCGTCGCAATGGCCGCCTGTCCGGCTGGCATCCCGC
GGACCTCGCGGGGCAGGTGCTCGACGCGCTTGTCGAGCGCAGCGGGGCCGACCCGAGCATGGTGGAGGACGTCATCATGG
GCTGCGTCGGCCAGGCGGGCGAGCAGTCTTCGAACATCGCGCGTGGCGCGGTCTTGGCTTCGAAGCTGCCGGAATCGGTG
CCGGCTACCTCGGTCGACCGCCAGTGCGGATCTTCGCAACAGGCGCTGCATTTCGCGGCGCAGGCCGTGATGTCGGGCAC
CATGGACGTGGTGATCGCCGCCGGCGTGGAAAGCATGTCCCGCGTGCCGATGGGCACCCCGAGCACGCTGCCGCGAAAGA
ACGGCCTGGGCCACTACGTCAGCCCGCAAATGCAGGCGCGCTATCCGGGCGTGGAGTTCAGCCAGTTCACCGGCGCGGAG
ATGATCGCGAAGAAGTACAAGCTCGATAAGACCGCGCTCGACGCCTATGCGCTGCGCAGCCACCAGCTCGCCGCCGCGGC
CACGAAGGCGGGGCGCTTCGAGCAGGAGATCCTGCCGCTGGCCGCGCGCATGAATGACGGCAGCGATCTCGGCGAGTTCC
ACACCATCGACGAAGGTATTCGTTTCGACGCGACGCTGGAGGGCATCGCCTCGGTAAAGCTGATCGCCGAAGGCGGTAGC
GTCACGGCGGCCACCGCCAGCCAGATCTGCGACGGCGCGAGCGGCGTCATGGTCGTCAACGAACGCGGGTTGAAGATGCT
GGGCGTCAAGCCGCTGGCACGCATCCACCATATGAGCGTGCTCGGACATGACCCGGTGGTCATGCTGGAGGCCCCGCTGC
CTGCTACCGAGCGCGCGCTGAAGCGGGCCGGCATGCGGATCGACGATATCGATCTCTACGAGGTCAACGAAGCGTTCGCG
CCGGTCCCCCTGGCCTGGCTGCAGGCGCTGGGCGCCGACGCGGATCGCCTGAACGTCAACGGCGGCGCGATCGCACTGGG
CCATCCGCTGGGCGGCTCGGGCACCAAGCTGATGACGACGTTGATCCATGCACTGCATCAGCGCGGCAAGCGCTACGGCC
TGCAGACCATGTGCGAGGGCGGCGGCATGGCCAACGTCACGATCGTCGAAAGGCTCTGA

Upstream 100 bases:

>100_bases
GCGATTCGCATGGCGGCGCGCTAACCGGCATTCCCGCTTTCAGGGTGGCGCCCCGACGGAAGATCGGGCGCCAGCCCGTC
ATCTCATCACTGGAGACACC

Downstream 100 bases:

>100_bases
CCGCGCGGCACACGTTTTCCAAAGCCCCGTCGGAGTGCACGCGTGGAGCGTGTCCGACGGGATTTTTTTGGTCTGCGGTG
CCATGTTCGGCGCGCCCTGG

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MSEAYIVSAVRTAGGRRNGRLSGWHPADLAGQVLDALVERSGADPSMVEDVIMGCVGQAGEQSSNIARGAVLASKLPESV
PATSVDRQCGSSQQALHFAAQAVMSGTMDVVIAAGVESMSRVPMGTPSTLPRKNGLGHYVSPQMQARYPGVEFSQFTGAE
MIAKKYKLDKTALDAYALRSHQLAAAATKAGRFEQEILPLAARMNDGSDLGEFHTIDEGIRFDATLEGIASVKLIAEGGS
VTAATASQICDGASGVMVVNERGLKMLGVKPLARIHHMSVLGHDPVVMLEAPLPATERALKRAGMRIDDIDLYEVNEAFA
PVPLAWLQALGADADRLNVNGGAIALGHPLGGSGTKLMTTLIHALHQRGKRYGLQTMCEGGGMANVTIVERL

Sequences:

>Translated_392_residues
MSEAYIVSAVRTAGGRRNGRLSGWHPADLAGQVLDALVERSGADPSMVEDVIMGCVGQAGEQSSNIARGAVLASKLPESV
PATSVDRQCGSSQQALHFAAQAVMSGTMDVVIAAGVESMSRVPMGTPSTLPRKNGLGHYVSPQMQARYPGVEFSQFTGAE
MIAKKYKLDKTALDAYALRSHQLAAAATKAGRFEQEILPLAARMNDGSDLGEFHTIDEGIRFDATLEGIASVKLIAEGGS
VTAATASQICDGASGVMVVNERGLKMLGVKPLARIHHMSVLGHDPVVMLEAPLPATERALKRAGMRIDDIDLYEVNEAFA
PVPLAWLQALGADADRLNVNGGAIALGHPLGGSGTKLMTTLIHALHQRGKRYGLQTMCEGGGMANVTIVERL
>Mature_391_residues
SEAYIVSAVRTAGGRRNGRLSGWHPADLAGQVLDALVERSGADPSMVEDVIMGCVGQAGEQSSNIARGAVLASKLPESVP
ATSVDRQCGSSQQALHFAAQAVMSGTMDVVIAAGVESMSRVPMGTPSTLPRKNGLGHYVSPQMQARYPGVEFSQFTGAEM
IAKKYKLDKTALDAYALRSHQLAAAATKAGRFEQEILPLAARMNDGSDLGEFHTIDEGIRFDATLEGIASVKLIAEGGSV
TAATASQICDGASGVMVVNERGLKMLGVKPLARIHHMSVLGHDPVVMLEAPLPATERALKRAGMRIDDIDLYEVNEAFAP
VPLAWLQALGADADRLNVNGGAIALGHPLGGSGTKLMTTLIHALHQRGKRYGLQTMCEGGGMANVTIVERL

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=396, Percent_Identity=35.8585858585859, Blast_Score=221, Evalue=6e-58,
Organism=Homo sapiens, GI148539872, Length=397, Percent_Identity=35.5163727959698, Blast_Score=211, Evalue=9e-55,
Organism=Homo sapiens, GI4557237, Length=410, Percent_Identity=33.9024390243902, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI4501853, Length=394, Percent_Identity=33.248730964467, Blast_Score=195, Evalue=5e-50,
Organism=Homo sapiens, GI4504327, Length=424, Percent_Identity=30.8962264150943, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI194353979, Length=89, Percent_Identity=42.6966292134831, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1787663, Length=405, Percent_Identity=40.9876543209877, Blast_Score=250, Evalue=9e-68,
Organism=Escherichia coli, GI48994986, Length=399, Percent_Identity=38.5964912280702, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI87082165, Length=398, Percent_Identity=40.4522613065327, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1788554, Length=397, Percent_Identity=38.2871536523929, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1788683, Length=417, Percent_Identity=30.9352517985612, Blast_Score=151, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI133906874, Length=394, Percent_Identity=36.8020304568528, Blast_Score=228, Evalue=5e-60,
Organism=Caenorhabditis elegans, GI17535921, Length=401, Percent_Identity=31.9201995012469, Blast_Score=187, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI25147385, Length=394, Percent_Identity=31.4720812182741, Blast_Score=181, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17551802, Length=423, Percent_Identity=30.4964539007092, Blast_Score=164, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI17535917, Length=403, Percent_Identity=28.287841191067, Blast_Score=140, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6325229, Length=403, Percent_Identity=33.4987593052109, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6322031, Length=390, Percent_Identity=32.3076923076923, Blast_Score=176, Evalue=7e-45,
Organism=Drosophila melanogaster, GI24655093, Length=395, Percent_Identity=38.9873417721519, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI17648125, Length=395, Percent_Identity=35.6962025316456, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24640423, Length=399, Percent_Identity=33.5839598997494, Blast_Score=191, Evalue=8e-49,
Organism=Drosophila melanogaster, GI17137578, Length=425, Percent_Identity=29.8823529411765, Blast_Score=158, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012805
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 41219; Mature: 41088

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAYIVSAVRTAGGRRNGRLSGWHPADLAGQVLDALVERSGADPSMVEDVIMGCVGQAG
CCCHHEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
EQSSNIARGAVLASKLPESVPATSVDRQCGSSQQALHFAAQAVMSGTMDVVIAAGVESMS
CCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCHHEEEECCHHHHH
RVPMGTPSTLPRKNGLGHYVSPQMQARYPGVEFSQFTGAEMIAKKYKLDKTALDAYALRS
CCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHCCHHHHHHHHHCCHHHHHHHHHHH
HQLAAAATKAGRFEQEILPLAARMNDGSDLGEFHTIDEGIRFDATLEGIASVKLIAEGGS
HHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCEECHHHHCCEEEEEEECCCC
VTAATASQICDGASGVMVVNERGLKMLGVKPLARIHHMSVLGHDPVVMLEAPLPATERAL
EEHHHHHHHCCCCCCEEEECCCCCEEECCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
KRAGMRIDDIDLYEVNEAFAPVPLAWLQALGADADRLNVNGGAIALGHPLGGSGTKLMTT
HHCCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCCEEECCCCEEEECCCCCCCCHHHHHH
LIHALHQRGKRYGLQTMCEGGGMANVTIVERL
HHHHHHHHHHHCCHHHHHCCCCEEEEEEEECC
>Mature Secondary Structure 
SEAYIVSAVRTAGGRRNGRLSGWHPADLAGQVLDALVERSGADPSMVEDVIMGCVGQAG
CCHHEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
EQSSNIARGAVLASKLPESVPATSVDRQCGSSQQALHFAAQAVMSGTMDVVIAAGVESMS
CCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCHHEEEECCHHHHH
RVPMGTPSTLPRKNGLGHYVSPQMQARYPGVEFSQFTGAEMIAKKYKLDKTALDAYALRS
CCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHCCHHHHHHHHHCCHHHHHHHHHHH
HQLAAAATKAGRFEQEILPLAARMNDGSDLGEFHTIDEGIRFDATLEGIASVKLIAEGGS
HHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCEECHHHHCCEEEEEEECCCC
VTAATASQICDGASGVMVVNERGLKMLGVKPLARIHHMSVLGHDPVVMLEAPLPATERAL
EEHHHHHHHCCCCCCEEEECCCCCEEECCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
KRAGMRIDDIDLYEVNEAFAPVPLAWLQALGADADRLNVNGGAIALGHPLGGSGTKLMTT
HHCCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCCEEECCCCEEEECCCCCCCCHHHHHH
LIHALHQRGKRYGLQTMCEGGGMANVTIVERL
HHHHHHHHHHHCCHHHHHCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA