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The map label for this gene is fabG2 [H]

Identifier: 94314352

GI number: 94314352

Start: 2179428

End: 2180210

Strand: Reverse

Name: fabG2 [H]

Synonym: Rmet_5433

Alternate gene names: 94314352

Gene position: 2180210-2179428 (Counterclockwise)

Preceding gene: 94314353

Following gene: 94314351

Centisome position: 84.5

GC content: 65.01

Gene sequence:

>783_bases
ATGAAACTCGATTCCAGCATTGCCGCGGTCATCACCGGTGGCGCTTCCGGCCTGGGTGCCGCCACCGCGCGACGCCTGGC
CGGCGCCGGCGTCAAGGTGGCGCTGTTCGATCTGAATGCGGAGCTGGGGGAAGCGCTGGCGCGCGAGCTTGGTGGCGTGT
TCTGCAAGGTTGACGTGACCAACGAGGAGCAGGTCGACGCCGCATTTGCCAAGGCGCGCGCGGCACATGGGCAGGAGCGC
GTGCTCGTGAACTGTGCCGGCACCGGTTTCTCCGCCAAGACGGCGGGCCGGGACAAATCGACGGGAGAAATCCGCCACTT
CCCCGTGGCCAACTTCAACCGGATAATTCAGATCAACCTGGTGGGCACCTTCAGCTGTATCGCCAAATCCGCTGCCGGAA
TGCTGACGCTGGACCCGCTCGATGACGGCGAACGCGGCACCATCATCAATACCGCGTCGGTGGCGGCGGAGGACGGGCAG
ATGGGCCAGGCGGCCTACAGTGCGTCAAAGGCGGCGATCACTGGCATGACGCTGCCGATCGCACGCGATCTGATGAGCGA
AGGCATCCGCGTGAACACGATCATGCCTGGCCTGTTCAACACGCCGCTGATGCAGGGCCTGCCCGACAACGTGAAGTCCG
CGCTGGCCGCGTCGGTGCCGTTTCCGAAGCGGCTCGGCGATCCGGACGAATACGCGCAACTGGCCGAGACCATGATCGTC
AACCGCTACTTCAACGGTGAATCCGTGCGTCTGGACGGCGCGATTCGCATGGCGGCGCGCTAA

Upstream 100 bases:

>100_bases
CGCAAGTATTTCATCTGAACCCCGCATTGCCGATGCCGGCGGCATGTGTCGCCGCCTGGCCAGATGCGACGGCAGTCTTC
CATTCACGCAAGGATATGCA

Downstream 100 bases:

>100_bases
CCGGCATTCCCGCTTTCAGGGTGGCGCCCCGACGGAAGATCGGGCGCCAGCCCGTCATCTCATCACTGGAGACACCATGT
CCGAAGCCTATATCGTTTCC

Product: Short-chain alcohol dehydrogenase/3-hydroxy-2-methylbutyryl-CoA dehydrogenase

Products: 3-oxoacyl-CoA; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKLDSSIAAVITGGASGLGAATARRLAGAGVKVALFDLNAELGEALARELGGVFCKVDVTNEEQVDAAFAKARAAHGQER
VLVNCAGTGFSAKTAGRDKSTGEIRHFPVANFNRIIQINLVGTFSCIAKSAAGMLTLDPLDDGERGTIINTASVAAEDGQ
MGQAAYSASKAAITGMTLPIARDLMSEGIRVNTIMPGLFNTPLMQGLPDNVKSALAASVPFPKRLGDPDEYAQLAETMIV
NRYFNGESVRLDGAIRMAAR

Sequences:

>Translated_260_residues
MKLDSSIAAVITGGASGLGAATARRLAGAGVKVALFDLNAELGEALARELGGVFCKVDVTNEEQVDAAFAKARAAHGQER
VLVNCAGTGFSAKTAGRDKSTGEIRHFPVANFNRIIQINLVGTFSCIAKSAAGMLTLDPLDDGERGTIINTASVAAEDGQ
MGQAAYSASKAAITGMTLPIARDLMSEGIRVNTIMPGLFNTPLMQGLPDNVKSALAASVPFPKRLGDPDEYAQLAETMIV
NRYFNGESVRLDGAIRMAAR
>Mature_260_residues
MKLDSSIAAVITGGASGLGAATARRLAGAGVKVALFDLNAELGEALARELGGVFCKVDVTNEEQVDAAFAKARAAHGQER
VLVNCAGTGFSAKTAGRDKSTGEIRHFPVANFNRIIQINLVGTFSCIAKSAAGMLTLDPLDDGERGTIINTASVAAEDGQ
MGQAAYSASKAAITGMTLPIARDLMSEGIRVNTIMPGLFNTPLMQGLPDNVKSALAASVPFPKRLGDPDEYAQLAETMIV
NRYFNGESVRLDGAIRMAAR

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4758504, Length=253, Percent_Identity=53.7549407114625, Blast_Score=245, Evalue=3e-65,
Organism=Homo sapiens, GI83715985, Length=253, Percent_Identity=50.9881422924901, Blast_Score=224, Evalue=6e-59,
Organism=Homo sapiens, GI15277342, Length=273, Percent_Identity=32.2344322344322, Blast_Score=117, Evalue=9e-27,
Organism=Homo sapiens, GI31542939, Length=245, Percent_Identity=30.6122448979592, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI40254992, Length=253, Percent_Identity=26.4822134387352, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI59889578, Length=221, Percent_Identity=29.4117647058824, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI5031737, Length=262, Percent_Identity=27.0992366412214, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI33667109, Length=201, Percent_Identity=30.3482587064677, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI224922801, Length=176, Percent_Identity=32.3863636363636, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787335, Length=253, Percent_Identity=29.6442687747036, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1789057, Length=239, Percent_Identity=31.3807531380753, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082100, Length=264, Percent_Identity=29.9242424242424, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI2367175, Length=259, Percent_Identity=30.1158301158301, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1790717, Length=258, Percent_Identity=26.7441860465116, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788459, Length=259, Percent_Identity=30.1158301158301, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787905, Length=241, Percent_Identity=28.6307053941909, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI87082160, Length=254, Percent_Identity=27.9527559055118, Blast_Score=65, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17538182, Length=253, Percent_Identity=49.802371541502, Blast_Score=244, Evalue=3e-65,
Organism=Caenorhabditis elegans, GI17555706, Length=260, Percent_Identity=28.8461538461538, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI25147288, Length=257, Percent_Identity=26.4591439688716, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17563726, Length=272, Percent_Identity=27.2058823529412, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI72000259, Length=274, Percent_Identity=27.3722627737226, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17538486, Length=269, Percent_Identity=29.7397769516729, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17560676, Length=258, Percent_Identity=24.031007751938, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17560150, Length=266, Percent_Identity=25.5639097744361, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17560332, Length=204, Percent_Identity=30.3921568627451, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17538480, Length=204, Percent_Identity=29.9019607843137, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17562906, Length=267, Percent_Identity=26.2172284644195, Blast_Score=64, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17562910, Length=267, Percent_Identity=26.5917602996255, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6320089, Length=245, Percent_Identity=26.530612244898, Blast_Score=63, Evalue=4e-11,
Organism=Drosophila melanogaster, GI17737361, Length=251, Percent_Identity=53.3864541832669, Blast_Score=254, Evalue=4e-68,
Organism=Drosophila melanogaster, GI24639444, Length=254, Percent_Identity=31.8897637795276, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI21355319, Length=257, Percent_Identity=28.0155642023346, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28571387, Length=218, Percent_Identity=31.1926605504587, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI28571526, Length=234, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21357041, Length=234, Percent_Identity=26.0683760683761, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.35

Molecular weight: Translated: 27088; Mature: 27088

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDSSIAAVITGGASGLGAATARRLAGAGVKVALFDLNAELGEALARELGGVFCKVDVT
CCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCEEEEEECC
NEEQVDAAFAKARAAHGQERVLVNCAGTGFSAKTAGRDKSTGEIRHFPVANFNRIIQINL
CCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEEEE
VGTFSCIAKSAAGMLTLDPLDDGERGTIINTASVAAEDGQMGQAAYSASKAAITGMTLPI
EEHHHHHHHHCCCEEEECCCCCCCCCCEEEEHHHCCCCCCCCHHHHHCCCHHEECCHHHH
ARDLMSEGIRVNTIMPGLFNTPLMQGLPDNVKSALAASVPFPKRLGDPDEYAQLAETMIV
HHHHHHCCEEEEEECCCHHCCHHHHCCCHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHH
NRYFNGESVRLDGAIRMAAR
HHEECCCEEEECCEEEECCC
>Mature Secondary Structure
MKLDSSIAAVITGGASGLGAATARRLAGAGVKVALFDLNAELGEALARELGGVFCKVDVT
CCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCEEEEEECC
NEEQVDAAFAKARAAHGQERVLVNCAGTGFSAKTAGRDKSTGEIRHFPVANFNRIIQINL
CCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEEEE
VGTFSCIAKSAAGMLTLDPLDDGERGTIINTASVAAEDGQMGQAAYSASKAAITGMTLPI
EEHHHHHHHHCCCEEEECCCCCCCCCCEEEEHHHCCCCCCCCHHHHHCCCHHEECCHHHH
ARDLMSEGIRVNTIMPGLFNTPLMQGLPDNVKSALAASVPFPKRLGDPDEYAQLAETMIV
HHHHHHCCEEEEEECCCHHCCHHHHCCCHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHH
NRYFNGESVRLDGAIRMAAR
HHEECCCEEEECCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-3-hydroxyacyl-CoA; NAD+

Specific reaction: (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]