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The map label for this gene is yafC [H]

Identifier: 94314284

GI number: 94314284

Start: 2086671

End: 2087573

Strand: Direct

Name: yafC [H]

Synonym: Rmet_5365

Alternate gene names: 94314284

Gene position: 2086671-2087573 (Clockwise)

Preceding gene: 94314281

Following gene: 291481567

Centisome position: 80.88

GC content: 64.45

Gene sequence:

>903_bases
ATGAAAACCACCATCGACGAACTGCTGGCCTTTGCCGCAGTAGTGGACTCCGGCTCGATCACGGCGGCGGCCGAACAACT
GGGGCTGACGATCTCGGCCACCAGCCGGACCTTGAGCCGTCTGGAGGAGAAGCTGAAGACCACGCTGCTGCGACGAACCA
CGCGGCGGCTGGAGCTGACCGAGGAAGGCGCCGCCTTCCTGGAGCACGCGCGCGCGATCCTGGCGATGGTCGACGAAGCG
GAAGAGCAGATGGCTGCACGGCAGATGCGACCCGTCGGGCGCTTGCGGGTGGACGCGGCCACGCCGTTCATGCTGCACGT
GATCGTGCCGCTGGTGGCGGGATTCCGCGCCAGCTACCCCGAGGTGGAACTGGAACTGAACTCGAACGAAGGCTTCATCG
ACCTGATCGAGAAACGAACCGACGTGGCGTTCCGCATCGGCCAGTTGAAGGACTCGACGCTGCATGCACGACCGATCGGT
ATCAGCCAGATCCGCGTGCTGGCCAGCCCGGAGTATCTGAAACGCCACGGCACGCCCACCAAGCCGGCGCAACTCGCGCA
ACATGCGCTGCTGGGCTTTACCCAGCCTGAATCACTGAACGACTGGCCGCTGCGCGACGACGCCGGTGGCGTCGTGCACA
TCAAGCCGACGATTGCATCGTCGAGCGGCGAAACGCTCAGGAGCCTGGCGCTGGCGGGCCAGGGAATCGTCTGCCTGTCG
GACTTCATGACGCTCGAGGACCGGCAGAGCGGGCGACTGGTGCAACTGTTTGCGAAGCAAACCCTGGACGTGCGCCAACC
GATCAACGCGGTCTACTACCGCAACACCGCGCTGGCGTCACGGATCACCTGCTTTCTCGACCACGTGGTCAGAACACTGG
GCAAGCATCCGTTCGAACGGTAG

Upstream 100 bases:

>100_bases
CCTGTGAAGGTTCATGCAATCTCGATGGACAAAGTTTGCCGTCCTTACCTTTGCTGATAAAGCCGTGTATAAACGAAATT
CATTTGATTTTTGATCAACA

Downstream 100 bases:

>100_bases
GAGTATCCGTCCCGTGCGGACATCCCCATACTTGACGCAAAATCAAGAGTATTTGACCAATACCGGTCTTAATCTGCAAA
GATAAACCGGGCAGACTGCG

Product: putative LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKTTIDELLAFAAVVDSGSITAAAEQLGLTISATSRTLSRLEEKLKTTLLRRTTRRLELTEEGAAFLEHARAILAMVDEA
EEQMAARQMRPVGRLRVDAATPFMLHVIVPLVAGFRASYPEVELELNSNEGFIDLIEKRTDVAFRIGQLKDSTLHARPIG
ISQIRVLASPEYLKRHGTPTKPAQLAQHALLGFTQPESLNDWPLRDDAGGVVHIKPTIASSSGETLRSLALAGQGIVCLS
DFMTLEDRQSGRLVQLFAKQTLDVRQPINAVYYRNTALASRITCFLDHVVRTLGKHPFER

Sequences:

>Translated_300_residues
MKTTIDELLAFAAVVDSGSITAAAEQLGLTISATSRTLSRLEEKLKTTLLRRTTRRLELTEEGAAFLEHARAILAMVDEA
EEQMAARQMRPVGRLRVDAATPFMLHVIVPLVAGFRASYPEVELELNSNEGFIDLIEKRTDVAFRIGQLKDSTLHARPIG
ISQIRVLASPEYLKRHGTPTKPAQLAQHALLGFTQPESLNDWPLRDDAGGVVHIKPTIASSSGETLRSLALAGQGIVCLS
DFMTLEDRQSGRLVQLFAKQTLDVRQPINAVYYRNTALASRITCFLDHVVRTLGKHPFER
>Mature_300_residues
MKTTIDELLAFAAVVDSGSITAAAEQLGLTISATSRTLSRLEEKLKTTLLRRTTRRLELTEEGAAFLEHARAILAMVDEA
EEQMAARQMRPVGRLRVDAATPFMLHVIVPLVAGFRASYPEVELELNSNEGFIDLIEKRTDVAFRIGQLKDSTLHARPIG
ISQIRVLASPEYLKRHGTPTKPAQLAQHALLGFTQPESLNDWPLRDDAGGVVHIKPTIASSSGETLRSLALAGQGIVCLS
DFMTLEDRQSGRLVQLFAKQTLDVRQPINAVYYRNTALASRITCFLDHVVRTLGKHPFER

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=297, Percent_Identity=46.1279461279461, Blast_Score=268, Evalue=4e-73,
Organism=Escherichia coli, GI1789639, Length=280, Percent_Identity=31.7857142857143, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI87081978, Length=296, Percent_Identity=28.3783783783784, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI145693193, Length=281, Percent_Identity=28.4697508896797, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1787128, Length=292, Percent_Identity=26.7123287671233, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789440, Length=296, Percent_Identity=28.7162162162162, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787589, Length=288, Percent_Identity=28.4722222222222, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1789173, Length=271, Percent_Identity=29.520295202952, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788481, Length=304, Percent_Identity=25.3289473684211, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1786448, Length=178, Percent_Identity=26.9662921348315, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787879, Length=250, Percent_Identity=28.8, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI145693105, Length=199, Percent_Identity=27.6381909547739, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082132, Length=266, Percent_Identity=24.4360902255639, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790262, Length=202, Percent_Identity=26.7326732673267, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33148; Mature: 33148

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTIDELLAFAAVVDSGSITAAAEQLGLTISATSRTLSRLEEKLKTTLLRRTTRRLELT
CCCCHHHHHHHHHHHCCCCHHHHHHHHCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEGAAFLEHARAILAMVDEAEEQMAARQMRPVGRLRVDAATPFMLHVIVPLVAGFRASYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCHHHHHHHHHHHHHHCCCCC
EVELELNSNEGFIDLIEKRTDVAFRIGQLKDSTLHARPIGISQIRVLASPEYLKRHGTPT
CEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEEECCCHHHHHHCCCCC
KPAQLAQHALLGFTQPESLNDWPLRDDAGGVVHIKPTIASSSGETLRSLALAGQGIVCLS
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCHHHHH
DFMTLEDRQSGRLVQLFAKQTLDVRQPINAVYYRNTALASRITCFLDHVVRTLGKHPFER
HHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKTTIDELLAFAAVVDSGSITAAAEQLGLTISATSRTLSRLEEKLKTTLLRRTTRRLELT
CCCCHHHHHHHHHHHCCCCHHHHHHHHCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEGAAFLEHARAILAMVDEAEEQMAARQMRPVGRLRVDAATPFMLHVIVPLVAGFRASYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCHHHHHHHHHHHHHHCCCCC
EVELELNSNEGFIDLIEKRTDVAFRIGQLKDSTLHARPIGISQIRVLASPEYLKRHGTPT
CEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEEECCCHHHHHHCCCCC
KPAQLAQHALLGFTQPESLNDWPLRDDAGGVVHIKPTIASSSGETLRSLALAGQGIVCLS
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCHHHHH
DFMTLEDRQSGRLVQLFAKQTLDVRQPINAVYYRNTALASRITCFLDHVVRTLGKHPFER
HHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]