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The map label for this gene is nemA [H]

Identifier: 94314282

GI number: 94314282

Start: 2084629

End: 2085750

Strand: Reverse

Name: nemA [H]

Synonym: Rmet_5363

Alternate gene names: 94314282

Gene position: 2085750-2084629 (Counterclockwise)

Preceding gene: 94314283

Following gene: 94314279

Centisome position: 80.84

GC content: 66.84

Gene sequence:

>1122_bases
ATGATGACCAGCACCAGCAATCCGCTGTTCCAGCCGTATTCGCTCGGCCCCCTCTCGCTGCCCAACCGCATCGTGATGCC
ACCGATGACGCGCTCGCGCGCCAGCCAGCCCGGTGACGTGCCCAATGCGCTGATGGCCGAGTACTACGCGCAACGCGCCA
GCGCCGGCCTGATCGTCAGCGAAGGCACGTGGATCTCGCCGCTCGGCAGGGGGTATGCGTGGACCCCCGGCATTCACACG
CCGGAGCAGGTCGCCGGCTGGCGCAAAGTCACCACTGCCGTCCATCAGGCCGGCGGGCGCATCTTCGCGCAGCTCTGGCA
CGTTGGCCGCCTGAGCCATATCAGCCTGCTGGATGGCCATGCGCCGGTATCGTCGTCAGCCATCCAGGCGCAGGGCGTGA
ACGTGTTCGTCGCGCAAAGCGATGGCAAGCCCGGCTTCGCCCAGGCGTCGCAGCCACGCGCGCTGTCGGTGGAGGAAATC
CACGCCATCGTCGACGACTTTCGCCAGGCCGCGCGCAATGCCATGGCGGCGGGCTTCGACGGTGTGGAACTGCACGCGGC
CAATGGCTATCTGGTCAACCAGTTCATCGACTCGGGCGCCAACGATCGCACCGACGAATACGGCGGTTCGCTGGAAAACC
GGCTGCGCTTTCTGGGCGAGGTGGCACAGGCGCTGATCGAAGGCACGGGTGGCAAAGACCGCGTCGGCATCCGTCTGGCG
CCGCTGACCACGCTGAATGGTTGCGTCGATGCCGATCCCGAGACTACCTATACCGCTGCGGCCAGGTTGCTCGGCGAGAT
CGGCGTGGGCTACATCCATATCGCGGAAGCAGACTGGGAAGATGCCCCGCATATGCCGACGGCCTTCAAGCAGCAACTGC
GCAAGGTCTATCCGGGCACGCTGATCTACGCCGGCAAGTACACGGCGGCGCGCGCCCAGGCTGCATTGCACGAAGGATGG
GCCGACCTGATCGCGTTTGGCCGCCCGTTCGTGGCCAACCCCGATCTGCCGGAGCGCCTGCGCACCGGCGCCACCCTCAA
CGCCCACGATCGCGACACGCTGTTCGGCGGCGACGCGCGTGGCCTGACCGACTATCCGCCACTTGTTGCCAAGGCGGCCT
GA

Upstream 100 bases:

>100_bases
AAGCTCGACGCCGACGACATGGCGCTGATCGCCTCGCTGGAACGCAACGGCCGTGAAGTCAGCCCTGAGGGCCTGGCTCC
GGTTTGGGACTAAGGAGATC

Downstream 100 bases:

>100_bases
GCGTCGGTCAGGAGACGGAAGATGAAGGCGCTGGCAAGGATCGCGCTGGCCGCGACGCCGCCGGCGTCGACCTGACGGGA
AATCGCCGACGCGCGGCATC

Product: N-ethylmaleimide reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MMTSTSNPLFQPYSLGPLSLPNRIVMPPMTRSRASQPGDVPNALMAEYYAQRASAGLIVSEGTWISPLGRGYAWTPGIHT
PEQVAGWRKVTTAVHQAGGRIFAQLWHVGRLSHISLLDGHAPVSSSAIQAQGVNVFVAQSDGKPGFAQASQPRALSVEEI
HAIVDDFRQAARNAMAAGFDGVELHAANGYLVNQFIDSGANDRTDEYGGSLENRLRFLGEVAQALIEGTGGKDRVGIRLA
PLTTLNGCVDADPETTYTAAARLLGEIGVGYIHIAEADWEDAPHMPTAFKQQLRKVYPGTLIYAGKYTAARAQAALHEGW
ADLIAFGRPFVANPDLPERLRTGATLNAHDRDTLFGGDARGLTDYPPLVAKAA

Sequences:

>Translated_373_residues
MMTSTSNPLFQPYSLGPLSLPNRIVMPPMTRSRASQPGDVPNALMAEYYAQRASAGLIVSEGTWISPLGRGYAWTPGIHT
PEQVAGWRKVTTAVHQAGGRIFAQLWHVGRLSHISLLDGHAPVSSSAIQAQGVNVFVAQSDGKPGFAQASQPRALSVEEI
HAIVDDFRQAARNAMAAGFDGVELHAANGYLVNQFIDSGANDRTDEYGGSLENRLRFLGEVAQALIEGTGGKDRVGIRLA
PLTTLNGCVDADPETTYTAAARLLGEIGVGYIHIAEADWEDAPHMPTAFKQQLRKVYPGTLIYAGKYTAARAQAALHEGW
ADLIAFGRPFVANPDLPERLRTGATLNAHDRDTLFGGDARGLTDYPPLVAKAA
>Mature_373_residues
MMTSTSNPLFQPYSLGPLSLPNRIVMPPMTRSRASQPGDVPNALMAEYYAQRASAGLIVSEGTWISPLGRGYAWTPGIHT
PEQVAGWRKVTTAVHQAGGRIFAQLWHVGRLSHISLLDGHAPVSSSAIQAQGVNVFVAQSDGKPGFAQASQPRALSVEEI
HAIVDDFRQAARNAMAAGFDGVELHAANGYLVNQFIDSGANDRTDEYGGSLENRLRFLGEVAQALIEGTGGKDRVGIRLA
PLTTLNGCVDADPETTYTAAARLLGEIGVGYIHIAEADWEDAPHMPTAFKQQLRKVYPGTLIYAGKYTAARAQAALHEGW
ADLIAFGRPFVANPDLPERLRTGATLNAHDRDTLFGGDARGLTDYPPLVAKAA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=366, Percent_Identity=46.7213114754098, Blast_Score=327, Evalue=6e-91,
Organism=Escherichia coli, GI1789463, Length=345, Percent_Identity=32.1739130434783, Blast_Score=129, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17559802, Length=273, Percent_Identity=31.5018315018315, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17559804, Length=284, Percent_Identity=30.6338028169014, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17565138, Length=309, Percent_Identity=27.831715210356, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17564188, Length=277, Percent_Identity=29.9638989169675, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=273, Percent_Identity=30.7692307692308, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17540738, Length=367, Percent_Identity=26.158038147139, Blast_Score=91, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6325086, Length=378, Percent_Identity=37.3015873015873, Blast_Score=223, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6321973, Length=387, Percent_Identity=37.2093023255814, Blast_Score=220, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39934; Mature: 39934

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTSTSNPLFQPYSLGPLSLPNRIVMPPMTRSRASQPGDVPNALMAEYYAQRASAGLIVS
CCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEE
EGTWISPLGRGYAWTPGIHTPEQVAGWRKVTTAVHQAGGRIFAQLWHVGRLSHISLLDGH
CCCEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEEEECCC
APVSSSAIQAQGVNVFVAQSDGKPGFAQASQPRALSVEEIHAIVDDFRQAARNAMAAGFD
CCCCCCCEEECCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
GVELHAANGYLVNQFIDSGANDRTDEYGGSLENRLRFLGEVAQALIEGTGGKDRVGIRLA
CEEEEECCCEEHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
PLTTLNGCVDADPETTYTAAARLLGEIGVGYIHIAEADWEDAPHMPTAFKQQLRKVYPGT
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCE
LIYAGKYTAARAQAALHEGWADLIAFGRPFVANPDLPERLRTGATLNAHDRDTLFGGDAR
EEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCCEECCCCC
GLTDYPPLVAKAA
CCCCCCCCCCCCC
>Mature Secondary Structure
MMTSTSNPLFQPYSLGPLSLPNRIVMPPMTRSRASQPGDVPNALMAEYYAQRASAGLIVS
CCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEE
EGTWISPLGRGYAWTPGIHTPEQVAGWRKVTTAVHQAGGRIFAQLWHVGRLSHISLLDGH
CCCEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEEEECCC
APVSSSAIQAQGVNVFVAQSDGKPGFAQASQPRALSVEEIHAIVDDFRQAARNAMAAGFD
CCCCCCCEEECCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
GVELHAANGYLVNQFIDSGANDRTDEYGGSLENRLRFLGEVAQALIEGTGGKDRVGIRLA
CEEEEECCCEEHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
PLTTLNGCVDADPETTYTAAARLLGEIGVGYIHIAEADWEDAPHMPTAFKQQLRKVYPGT
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCE
LIYAGKYTAARAQAALHEGWADLIAFGRPFVANPDLPERLRTGATLNAHDRDTLFGGDAR
EEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCCEECCCCC
GLTDYPPLVAKAA
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]