| Definition | Cupriavidus metallidurans CH34 megaplasmid, complete sequence. |
|---|---|
| Accession | NC_007974 |
| Length | 2,580,084 |
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The map label for this gene is dkgB [H]
Identifier: 94314283
GI number: 94314283
Start: 2085758
End: 2086561
Strand: Reverse
Name: dkgB [H]
Synonym: Rmet_5364
Alternate gene names: 94314283
Gene position: 2086561-2085758 (Counterclockwise)
Preceding gene: 94314286
Following gene: 94314282
Centisome position: 80.87
GC content: 64.8
Gene sequence:
>804_bases ATGAGCATTCCTTCGTTTGGCGTTGGCACCTTCCGCCTGACTGGCCAGGTCGTTGTGGACTCGGTGCGCAATGCGCTGGA CGTTGGCTACCGCGCCATCGACACCGCGCAGATCTATGGCAACGAGGCCGATGTGGGCCAGGCCATCGCGGAAAGCGGTG TGGAGCGCGGCGAACTGTTCGTGACCACCAAGATCTGGACGGCGAACTACGCCGCCGACAAGCTGGTGCCGAGCCTGCGT GAAAGCCTGGACAAGCTGCGCACCGACTACGTCGACCTGACGCTGATTCACTGGCCGGCGCCGGGCAACGGCGTGGCGCT GTCCGAGTACATGGCCGCGCTTGCCGAAGCCAAGGCGCTCGGGCTGACGCGCCAGATCGGCGTGTCCAACTTCAATATCG AACTGACCCGCCAGGCGATCGAGGCCGTTGGCAAGGGCGAGATCGCCACCAACCAGATTGAACTGAGCCCCTATCTGCAG AGCCGCAAGCTCACCGCGTTCCTGAAAGAACAGGGCATCGCCGTCACGTCGTACATGACGCTGGCCTACGGCAAGGTGCT GAAGGACCCGGTGCTGGGGCAGATTGCCGCGAAGCACCACGCCACCGTGGCGCAGGTGGCGCTGGCATGGGCGCTGCAAC TCGGCTACGCGGTGATTCCGTCGTCGACCAAGCGCGAGAACCTGGCCAGCAACCTGCTGGCGCGCGACCTGAAGCTCGAC GCCGACGACATGGCGCTGATCGCCTCGCTGGAACGCAACGGCCGTGAAGTCAGCCCTGAGGGCCTGGCTCCGGTTTGGGA CTAA
Upstream 100 bases:
>100_bases AAAATCAAATGAATTTCGTTTATACACGGCTTTATCAGCAAAGGTAAGGACGGCAAACTTTGTCCATCGAGATTGCATGA ACCTTCACAGGAGTTACACC
Downstream 100 bases:
>100_bases GGAGATCATGATGACCAGCACCAGCAATCCGCTGTTCCAGCCGTATTCGCTCGGCCCCCTCTCGCTGCCCAACCGCATCG TGATGCCACCGATGACGCGC
Product: 2,5-diketo-D-gluconate reductase B
Products: NA
Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]
Number of amino acids: Translated: 267; Mature: 266
Protein sequence:
>267_residues MSIPSFGVGTFRLTGQVVVDSVRNALDVGYRAIDTAQIYGNEADVGQAIAESGVERGELFVTTKIWTANYAADKLVPSLR ESLDKLRTDYVDLTLIHWPAPGNGVALSEYMAALAEAKALGLTRQIGVSNFNIELTRQAIEAVGKGEIATNQIELSPYLQ SRKLTAFLKEQGIAVTSYMTLAYGKVLKDPVLGQIAAKHHATVAQVALAWALQLGYAVIPSSTKRENLASNLLARDLKLD ADDMALIASLERNGREVSPEGLAPVWD
Sequences:
>Translated_267_residues MSIPSFGVGTFRLTGQVVVDSVRNALDVGYRAIDTAQIYGNEADVGQAIAESGVERGELFVTTKIWTANYAADKLVPSLR ESLDKLRTDYVDLTLIHWPAPGNGVALSEYMAALAEAKALGLTRQIGVSNFNIELTRQAIEAVGKGEIATNQIELSPYLQ SRKLTAFLKEQGIAVTSYMTLAYGKVLKDPVLGQIAAKHHATVAQVALAWALQLGYAVIPSSTKRENLASNLLARDLKLD ADDMALIASLERNGREVSPEGLAPVWD >Mature_266_residues SIPSFGVGTFRLTGQVVVDSVRNALDVGYRAIDTAQIYGNEADVGQAIAESGVERGELFVTTKIWTANYAADKLVPSLRE SLDKLRTDYVDLTLIHWPAPGNGVALSEYMAALAEAKALGLTRQIGVSNFNIELTRQAIEAVGKGEIATNQIELSPYLQS RKLTAFLKEQGIAVTSYMTLAYGKVLKDPVLGQIAAKHHATVAQVALAWALQLGYAVIPSSTKRENLASNLLARDLKLDA DDMALIASLERNGREVSPEGLAPVWD
Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI310109922, Length=272, Percent_Identity=33.0882352941176, Blast_Score=153, Evalue=2e-37, Organism=Homo sapiens, GI310109920, Length=289, Percent_Identity=33.2179930795848, Blast_Score=152, Evalue=3e-37, Organism=Homo sapiens, GI45446745, Length=289, Percent_Identity=33.2179930795848, Blast_Score=152, Evalue=4e-37, Organism=Homo sapiens, GI4503285, Length=289, Percent_Identity=33.2179930795848, Blast_Score=152, Evalue=4e-37, Organism=Homo sapiens, GI223468663, Length=286, Percent_Identity=32.8671328671329, Blast_Score=150, Evalue=1e-36, Organism=Homo sapiens, GI24497585, Length=291, Percent_Identity=32.9896907216495, Blast_Score=149, Evalue=2e-36, Organism=Homo sapiens, GI5453543, Length=289, Percent_Identity=32.8719723183391, Blast_Score=148, Evalue=4e-36, Organism=Homo sapiens, GI24497577, Length=292, Percent_Identity=32.1917808219178, Blast_Score=145, Evalue=4e-35, Organism=Homo sapiens, GI5174391, Length=292, Percent_Identity=32.1917808219178, Blast_Score=145, Evalue=4e-35, Organism=Homo sapiens, GI24497583, Length=297, Percent_Identity=32.3232323232323, Blast_Score=144, Evalue=8e-35, Organism=Homo sapiens, GI93277124, Length=298, Percent_Identity=30.2013422818792, Blast_Score=144, Evalue=9e-35, Organism=Homo sapiens, GI4502049, Length=285, Percent_Identity=35.4385964912281, Blast_Score=144, Evalue=1e-34, Organism=Homo sapiens, GI5174695, Length=293, Percent_Identity=32.0819112627986, Blast_Score=143, Evalue=2e-34, Organism=Homo sapiens, GI291291012, Length=271, Percent_Identity=33.210332103321, Blast_Score=141, Evalue=5e-34, Organism=Homo sapiens, GI300116273, Length=268, Percent_Identity=32.089552238806, Blast_Score=132, Evalue=4e-31, Organism=Homo sapiens, GI300116271, Length=278, Percent_Identity=30.2158273381295, Blast_Score=118, Evalue=4e-27, Organism=Homo sapiens, GI310109924, Length=154, Percent_Identity=33.1168831168831, Blast_Score=86, Evalue=3e-17, Organism=Homo sapiens, GI310109926, Length=104, Percent_Identity=36.5384615384615, Blast_Score=78, Evalue=7e-15, Organism=Homo sapiens, GI207028673, Length=104, Percent_Identity=36.5384615384615, Blast_Score=78, Evalue=8e-15, Organism=Escherichia coli, GI1786400, Length=267, Percent_Identity=67.7902621722846, Blast_Score=369, Evalue=1e-104, Organism=Escherichia coli, GI87082198, Length=263, Percent_Identity=36.8821292775665, Blast_Score=171, Evalue=5e-44, Organism=Escherichia coli, GI1788081, Length=265, Percent_Identity=29.4339622641509, Blast_Score=100, Evalue=8e-23, Organism=Escherichia coli, GI1788070, Length=313, Percent_Identity=24.9201277955272, Blast_Score=69, Evalue=4e-13, Organism=Escherichia coli, GI1787674, Length=281, Percent_Identity=26.6903914590747, Blast_Score=68, Evalue=6e-13, Organism=Escherichia coli, GI48994888, Length=267, Percent_Identity=27.7153558052434, Blast_Score=67, Evalue=2e-12, Organism=Caenorhabditis elegans, GI17561298, Length=275, Percent_Identity=31.6363636363636, Blast_Score=148, Evalue=3e-36, Organism=Caenorhabditis elegans, GI17550248, Length=286, Percent_Identity=35.3146853146853, Blast_Score=147, Evalue=6e-36, Organism=Caenorhabditis elegans, GI71998625, Length=266, Percent_Identity=33.8345864661654, Blast_Score=143, Evalue=9e-35, Organism=Caenorhabditis elegans, GI17566692, Length=286, Percent_Identity=32.8671328671329, Blast_Score=137, Evalue=7e-33, Organism=Caenorhabditis elegans, GI17537077, Length=288, Percent_Identity=33.3333333333333, Blast_Score=135, Evalue=1e-32, Organism=Caenorhabditis elegans, GI17537075, Length=290, Percent_Identity=33.1034482758621, Blast_Score=134, Evalue=5e-32, Organism=Caenorhabditis elegans, GI17552492, Length=265, Percent_Identity=29.811320754717, Blast_Score=131, Evalue=4e-31, Organism=Caenorhabditis elegans, GI17537079, Length=295, Percent_Identity=29.4915254237288, Blast_Score=128, Evalue=4e-30, Organism=Caenorhabditis elegans, GI17564128, Length=290, Percent_Identity=30.6896551724138, Blast_Score=127, Evalue=7e-30, Organism=Caenorhabditis elegans, GI17538386, Length=267, Percent_Identity=31.8352059925094, Blast_Score=124, Evalue=5e-29, Organism=Caenorhabditis elegans, GI17561300, Length=274, Percent_Identity=29.5620437956204, Blast_Score=124, Evalue=6e-29, Organism=Caenorhabditis elegans, GI17562292, Length=269, Percent_Identity=31.2267657992565, Blast_Score=112, Evalue=3e-25, Organism=Saccharomyces cerevisiae, GI6320576, Length=279, Percent_Identity=35.84229390681, Blast_Score=156, Evalue=3e-39, Organism=Saccharomyces cerevisiae, GI6324694, Length=285, Percent_Identity=34.3859649122807, Blast_Score=149, Evalue=4e-37, Organism=Saccharomyces cerevisiae, GI6322556, Length=255, Percent_Identity=30.1960784313725, Blast_Score=131, Evalue=1e-31, Organism=Saccharomyces cerevisiae, GI6321896, Length=306, Percent_Identity=30.0653594771242, Blast_Score=127, Evalue=1e-30, Organism=Saccharomyces cerevisiae, GI6319625, Length=267, Percent_Identity=29.9625468164794, Blast_Score=101, Evalue=1e-22, Organism=Saccharomyces cerevisiae, GI6320079, Length=236, Percent_Identity=31.3559322033898, Blast_Score=89, Evalue=7e-19, Organism=Drosophila melanogaster, GI24657054, Length=288, Percent_Identity=36.8055555555556, Blast_Score=156, Evalue=1e-38, Organism=Drosophila melanogaster, GI24662789, Length=288, Percent_Identity=34.375, Blast_Score=156, Evalue=1e-38, Organism=Drosophila melanogaster, GI24644950, Length=295, Percent_Identity=33.5593220338983, Blast_Score=145, Evalue=4e-35, Organism=Drosophila melanogaster, GI24663317, Length=292, Percent_Identity=33.2191780821918, Blast_Score=140, Evalue=7e-34, Organism=Drosophila melanogaster, GI20129731, Length=286, Percent_Identity=33.5664335664336, Blast_Score=139, Evalue=2e-33, Organism=Drosophila melanogaster, GI221378297, Length=315, Percent_Identity=31.4285714285714, Blast_Score=137, Evalue=7e-33, Organism=Drosophila melanogaster, GI21356425, Length=284, Percent_Identity=33.0985915492958, Blast_Score=136, Evalue=2e-32, Organism=Drosophila melanogaster, GI24662785, Length=290, Percent_Identity=34.4827586206897, Blast_Score=123, Evalue=2e-28, Organism=Drosophila melanogaster, GI24662781, Length=290, Percent_Identity=33.7931034482759, Blast_Score=118, Evalue=4e-27, Organism=Drosophila melanogaster, GI45553081, Length=285, Percent_Identity=28.0701754385965, Blast_Score=108, Evalue=5e-24, Organism=Drosophila melanogaster, GI281366140, Length=259, Percent_Identity=27.7992277992278, Blast_Score=94, Evalue=1e-19,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: =1.1.1.274 [H]
Molecular weight: Translated: 28784; Mature: 28653
Theoretical pI: Translated: 5.14; Mature: 5.14
Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.0 %Cys (Translated Protein) 1.5 %Met (Translated Protein) 1.5 %Cys+Met (Translated Protein) 0.0 %Cys (Mature Protein) 1.1 %Met (Mature Protein) 1.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSIPSFGVGTFRLTGQVVVDSVRNALDVGYRAIDTAQIYGNEADVGQAIAESGVERGELF CCCCCCCCCEEEEHHHHHHHHHHHHHHHCHHHEEHHHHCCCCHHHHHHHHHCCCCCCEEE VTTKIWTANYAADKLVPSLRESLDKLRTDYVDLTLIHWPAPGNGVALSEYMAALAEAKAL EEEEEEECCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHH GLTRQIGVSNFNIELTRQAIEAVGKGEIATNQIELSPYLQSRKLTAFLKEQGIAVTSYMT CCHHHCCCCCCCHHHHHHHHHHHCCCCEECCEEEECHHHHHHHHHHHHHHCCCCHHHHHH LAYGKVLKDPVLGQIAAKHHATVAQVALAWALQLGYAVIPSSTKRENLASNLLARDLKLD HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHCCCC ADDMALIASLERNGREVSPEGLAPVWD CHHHHHHHHHHHCCCCCCCCCCCCCCC >Mature Secondary Structure SIPSFGVGTFRLTGQVVVDSVRNALDVGYRAIDTAQIYGNEADVGQAIAESGVERGELF CCCCCCCCEEEEHHHHHHHHHHHHHHHCHHHEEHHHHCCCCHHHHHHHHHCCCCCCEEE VTTKIWTANYAADKLVPSLRESLDKLRTDYVDLTLIHWPAPGNGVALSEYMAALAEAKAL EEEEEEECCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHH GLTRQIGVSNFNIELTRQAIEAVGKGEIATNQIELSPYLQSRKLTAFLKEQGIAVTSYMT CCHHHCCCCCCCHHHHHHHHHHHCCCCEECCEEEECHHHHHHHHHHHHHHCCCCHHHHHH LAYGKVLKDPVLGQIAAKHHATVAQVALAWALQLGYAVIPSSTKRENLASNLLARDLKLD HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHCCCC ADDMALIASLERNGREVSPEGLAPVWD CHHHHHHHHHHHCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 11586360; 12142430 [H]