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The map label for this gene is paaF [C]

Identifier: 94314280

GI number: 94314280

Start: 2082483

End: 2083304

Strand: Direct

Name: paaF [C]

Synonym: Rmet_5361

Alternate gene names: 94314280

Gene position: 2082483-2083304 (Clockwise)

Preceding gene: 94314278

Following gene: 94314281

Centisome position: 80.71

GC content: 67.4

Gene sequence:

>822_bases
ATGACGCCCCCTAACGCTTCGTCTACCGCTTCCTCTACCGTCTCCTCTACCAACGGCCAGGATTCCCTTCAGGTCACGCG
CGACGGACATGTCGTCCAGGTCCTGATTCGCAGGCCGCCCCATAACCACCTCGACCCGGCATTCGTCGGCCTGCTGGCGG
ACACGCTCGAACAACTCGATGGCGACGACGAATGCCGCGCCATCGTGCTGGCGGCCGAAGGCAAGTCCTTCTGCGCCGGC
GCGGACTTCTCCGGCCAGCAAGCATCGTCATCGGTCAGCCGCGACCCGAGCCCGTTCTACAAGCAGGCCATGCGGCTCTT
CCGCACGCGCAAGCCGATCGTCGCCGCAGTACACGGCGCGGCCGTGGGCGCCGGCGTAGGGTTGTCGCTCGTGGCGGACT
TCCGCGTCACCTGCCCCGAGGCGCGGTTCAGCGTCAACTTCAACCGGCTGGGTTTTCATGCCGGCTTCGGCCTGAGCCTG
ACCCTGCCCCGCCTGATCGGGGTCCAGCAGGCATCGCTGCTGCTATACACGGGGCGTCGCATTGACGGCGAGACCGCCGT
GCGCATCGGACTGGCTGACGAACTCGTACCGGCCGACCAGGTTCTGGCCCGCGCCCATGCGCTGGCCACCGAGATCGCAA
TCTCGGCCCCGCTCGCCGTGGAAAGCACGCGCGCCGCGCTGCGCGAGGGGCTGGCGGATGCGATCTACGCCGCCAACCAG
AAGGAGCTGGCTATCCAGCTCACGCAATTCCCGACCGAGGATTTTCGCGAAGGCGTGGCAGCCATGGCCGAACGCCGTCC
GCCGAACTTCCGTCGTCGCTGA

Upstream 100 bases:

>100_bases
TATTGCGCTTCAAATTCATATTGAATAAGGCATAACGCAGTATGCAGCATTTCAACCCCGCCCGGCGGGGAAACCTATCC
ACAGAACCGAGCCCATCGAC

Downstream 100 bases:

>100_bases
TACTCAATGGAGCGAATCTACGTGACCGTTTCAACCAAAGCAGATGTCGGACCACTGTCCGGCGTGCGCGTGCTCGATCT
GTCCGCCGTGGTGCTGGGCC

Product: putative Enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MTPPNASSTASSTVSSTNGQDSLQVTRDGHVVQVLIRRPPHNHLDPAFVGLLADTLEQLDGDDECRAIVLAAEGKSFCAG
ADFSGQQASSSVSRDPSPFYKQAMRLFRTRKPIVAAVHGAAVGAGVGLSLVADFRVTCPEARFSVNFNRLGFHAGFGLSL
TLPRLIGVQQASLLLYTGRRIDGETAVRIGLADELVPADQVLARAHALATEIAISAPLAVESTRAALREGLADAIYAANQ
KELAIQLTQFPTEDFREGVAAMAERRPPNFRRR

Sequences:

>Translated_273_residues
MTPPNASSTASSTVSSTNGQDSLQVTRDGHVVQVLIRRPPHNHLDPAFVGLLADTLEQLDGDDECRAIVLAAEGKSFCAG
ADFSGQQASSSVSRDPSPFYKQAMRLFRTRKPIVAAVHGAAVGAGVGLSLVADFRVTCPEARFSVNFNRLGFHAGFGLSL
TLPRLIGVQQASLLLYTGRRIDGETAVRIGLADELVPADQVLARAHALATEIAISAPLAVESTRAALREGLADAIYAANQ
KELAIQLTQFPTEDFREGVAAMAERRPPNFRRR
>Mature_272_residues
TPPNASSTASSTVSSTNGQDSLQVTRDGHVVQVLIRRPPHNHLDPAFVGLLADTLEQLDGDDECRAIVLAAEGKSFCAGA
DFSGQQASSSVSRDPSPFYKQAMRLFRTRKPIVAAVHGAAVGAGVGLSLVADFRVTCPEARFSVNFNRLGFHAGFGLSLT
LPRLIGVQQASLLLYTGRRIDGETAVRIGLADELVPADQVLARAHALATEIAISAPLAVESTRAALREGLADAIYAANQK
ELAIQLTQFPTEDFREGVAAMAERRPPNFRRR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=263, Percent_Identity=29.277566539924, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI194097323, Length=246, Percent_Identity=27.6422764227642, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI4502327, Length=258, Percent_Identity=27.906976744186, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI150378541, Length=249, Percent_Identity=30.1204819277108, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI68989263, Length=169, Percent_Identity=33.1360946745562, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI62530384, Length=230, Percent_Identity=28.2608695652174, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI37594469, Length=192, Percent_Identity=27.6041666666667, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI37594471, Length=192, Percent_Identity=27.6041666666667, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4757966, Length=240, Percent_Identity=23.3333333333333, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI51538581, Length=240, Percent_Identity=23.3333333333333, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI25453481, Length=240, Percent_Identity=23.3333333333333, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI51538573, Length=240, Percent_Identity=23.3333333333333, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI116235487, Length=194, Percent_Identity=25.7731958762887, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1787659, Length=254, Percent_Identity=31.1023622047244, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI221142681, Length=259, Percent_Identity=29.3436293436293, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788597, Length=253, Percent_Identity=27.2727272727273, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790281, Length=192, Percent_Identity=28.6458333333333, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1787660, Length=250, Percent_Identity=27.6, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788682, Length=187, Percent_Identity=30.4812834224599, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI87082183, Length=246, Percent_Identity=26.8292682926829, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=28.2868525896414, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17560910, Length=195, Percent_Identity=31.7948717948718, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI25145438, Length=242, Percent_Identity=27.2727272727273, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=261, Percent_Identity=26.0536398467433, Blast_Score=90, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17540714, Length=211, Percent_Identity=26.0663507109005, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17558304, Length=181, Percent_Identity=30.3867403314917, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=255, Percent_Identity=24.3137254901961, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=217, Percent_Identity=26.7281105990783, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=263, Percent_Identity=25.8555133079848, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17508951, Length=213, Percent_Identity=30.0469483568075, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25144276, Length=213, Percent_Identity=30.0469483568075, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=213, Percent_Identity=30.0469483568075, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17549921, Length=183, Percent_Identity=27.3224043715847, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20129971, Length=270, Percent_Identity=30, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24653477, Length=270, Percent_Identity=30, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24653139, Length=260, Percent_Identity=28.4615384615385, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19920382, Length=252, Percent_Identity=31.7460317460317, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21357171, Length=199, Percent_Identity=28.1407035175879, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24650670, Length=246, Percent_Identity=29.2682926829268, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19922422, Length=276, Percent_Identity=23.9130434782609, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29158; Mature: 29027

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPPNASSTASSTVSSTNGQDSLQVTRDGHVVQVLIRRPPHNHLDPAFVGLLADTLEQLD
CCCCCCCCCHHHHHCCCCCCCCEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCC
GDDECRAIVLAAEGKSFCAGADFSGQQASSSVSRDPSPFYKQAMRLFRTRKPIVAAVHGA
CCCCCEEEEEEECCCCEECCCCCCCCHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEHHHH
AVGAGVGLSLVADFRVTCPEARFSVNFNRLGFHAGFGLSLTLPRLIGVQQASLLLYTGRR
HHHCCCCHHEEECCEEECCCCEEEEEHHHEEEECCCCCEEHHHHHHCHHHCEEEEEECCE
IDGETAVRIGLADELVPADQVLARAHALATEIAISAPLAVESTRAALREGLADAIYAANQ
ECCCCEEEEECHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
KELAIQLTQFPTEDFREGVAAMAERRPPNFRRR
CCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TPPNASSTASSTVSSTNGQDSLQVTRDGHVVQVLIRRPPHNHLDPAFVGLLADTLEQLD
CCCCCCCCHHHHHCCCCCCCCEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCC
GDDECRAIVLAAEGKSFCAGADFSGQQASSSVSRDPSPFYKQAMRLFRTRKPIVAAVHGA
CCCCCEEEEEEECCCCEECCCCCCCCHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEHHHH
AVGAGVGLSLVADFRVTCPEARFSVNFNRLGFHAGFGLSLTLPRLIGVQQASLLLYTGRR
HHHCCCCHHEEECCEEECCCCEEEEEHHHEEEECCCCCEEHHHHHHCHHHCEEEEEECCE
IDGETAVRIGLADELVPADQVLARAHALATEIAISAPLAVESTRAALREGLADAIYAANQ
ECCCCEEEEECHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
KELAIQLTQFPTEDFREGVAAMAERRPPNFRRR
CCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA