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The map label for this gene is zniS [H]

Identifier: 94314241

GI number: 94314241

Start: 2031611

End: 2033200

Strand: Reverse

Name: zniS [H]

Synonym: Rmet_5322

Alternate gene names: 94314241

Gene position: 2033200-2031611 (Counterclockwise)

Preceding gene: 94314243

Following gene: 94314239

Centisome position: 78.8

GC content: 64.28

Gene sequence:

>1590_bases
GTGGGCAGGCTTTCCGTAGCCCCGCTGGCACAGCTCAATCGATTGCGCGTGCGTCTGTCGCTGGTGTTCGTCCTGTTCCT
GCTGCTCGTAATCGGGCTGGGCGCTTTCAGCATCAACCGGCTCAGCGCCTTTCACAACGTCGCGGCCGAGATCAGTGGCC
GCTGGCTGCTGAGCAACCGCATCGCAGGCGATCTCAACAACAGCATCTCGGACTTCCGTGCTACCGAGGGTGAACTGCTG
ACCGCGCGTCGCGGCAAGGATCGGGGGCGCGCGGAAGGTTCGCTCGCGGTACTCGATGAAGAAATCGCGGATGCCAAACG
GCGCTACGAAACGATCGAGCATGACCCCATCGAACTGGACCTCTACCGGCAGTTCAGCGAGCAGTGGCGGGCATATCGCG
AACTGGCCAACCGAGTGCTGGTGCTTTCCCAGCGCGATGACAAGACCGACGCCATCGCGCTTTACTACCAGGAGTCCCGA
CGCGCCTTCACTGTGGCCAATGCCACGCTGGCCACGCTGACCGAGCGCAACGTCACCGGCGCGAACGAGGCTACCCAGGC
GGAGGCCGTAGCCTACCGGCATGCCAGGGACCTGATCGTGAACGCGATCATCGTGGCGGCGCTGCTGATGATTGCCGGCG
TGCTTTACTTCGTCACGGCTGTGACCAATCCAATCGCGGAACTGGTTGACCGGATGCACCGGATCTCCGACAACGAAACC
GAGATCGACATCCCATCGACCGAGCGCCCCGACGAAGTGGGCGAGATCGCTCGCGCCGTGGTCCGGTTCCGCGATAACAC
GGTGGCGCTGGCGCAAAGCCGCGCCACGCTGGCCGAGCAGGCCCGCACGCTGGAAATCACGCTGGCCAACGAGCGGCGCA
TGGCGGAGCTGCAGCGCAACTTCGTGTCGATGGTGTCGCACGAATTCCGCACCCCGTTGACCGTGATCGACGGCTACGCG
CAGCGCCTGAGTCATATCAAGCCGCCGCTGTCGCTTGAGGCGGTGCAGGATCGTTCGAGCAGAATCCGCGCGGCGGTGCG
GCGGATGACCGCGCTGATCGAAGATATGCTCGGGGCATCGCAGGTGCTTGACCTGGCAACACCGGGGGCGTTTCAACGCA
CGGAGTTCTCGTTACACCTGCTGGTGCACGAGGTCTGCGAGATGCATCGCGAAAGTTCGCCGGATGCGCAGATTGTCGAG
CAGTGGCACGCCGACGTACCCGAGGTTTTCCATGGCGATGCGCGTTTGCTGTTCCAGGCCGTGAGCAATCTGATCGGGAA
CGCCGTCAAGTACTCGCCGCCGAAGACGCCGGTCAGCGTGGATGTATCGATGGAATCGGGCGCGATCCTGATTTGCGTCG
CGGACCAGGGCATCGGCATTCCGGAGAAGGATCTCGGCCACGTGTTCGAGCGATACGTGCGTGGCGCCAATGTGACCGGC
ACCGCCGGCGCGGGCGTGGGTCTCTATATCGTCCGGCTGGCCGCCGAATTGCATGGCGGCACGGTGTCGGTGCGAAGCGC
GGAAGGCACGGGCTCGCGCTTCTTGCTCAGGCTGCCAGAAGCGCCAGCGCCAGCGCCGCTCAGCGCGTAG

Upstream 100 bases:

>100_bases
CTACTGTAGGGGAGCGGGCCGCAACCCGTCAATCGAACGGCATCTTGCAACTTCAAGGTAATGTATGCGCCACTTGTCTA
GCGCAGAGGGAAAGATAGCG

Downstream 100 bases:

>100_bases
CGACTTCGGCGCGGGCCGGCTTGCCCGCGTCACCCACCTGCGTACCTTCGGCCTGGCGATTCCACCAGCCGCCACCCAGA
GACTGGTAGAGCGCCACGGT

Product: Histidine kinase, sensor, two components regulatory system (zniS)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MGRLSVAPLAQLNRLRVRLSLVFVLFLLLVIGLGAFSINRLSAFHNVAAEISGRWLLSNRIAGDLNNSISDFRATEGELL
TARRGKDRGRAEGSLAVLDEEIADAKRRYETIEHDPIELDLYRQFSEQWRAYRELANRVLVLSQRDDKTDAIALYYQESR
RAFTVANATLATLTERNVTGANEATQAEAVAYRHARDLIVNAIIVAALLMIAGVLYFVTAVTNPIAELVDRMHRISDNET
EIDIPSTERPDEVGEIARAVVRFRDNTVALAQSRATLAEQARTLEITLANERRMAELQRNFVSMVSHEFRTPLTVIDGYA
QRLSHIKPPLSLEAVQDRSSRIRAAVRRMTALIEDMLGASQVLDLATPGAFQRTEFSLHLLVHEVCEMHRESSPDAQIVE
QWHADVPEVFHGDARLLFQAVSNLIGNAVKYSPPKTPVSVDVSMESGAILICVADQGIGIPEKDLGHVFERYVRGANVTG
TAGAGVGLYIVRLAAELHGGTVSVRSAEGTGSRFLLRLPEAPAPAPLSA

Sequences:

>Translated_529_residues
MGRLSVAPLAQLNRLRVRLSLVFVLFLLLVIGLGAFSINRLSAFHNVAAEISGRWLLSNRIAGDLNNSISDFRATEGELL
TARRGKDRGRAEGSLAVLDEEIADAKRRYETIEHDPIELDLYRQFSEQWRAYRELANRVLVLSQRDDKTDAIALYYQESR
RAFTVANATLATLTERNVTGANEATQAEAVAYRHARDLIVNAIIVAALLMIAGVLYFVTAVTNPIAELVDRMHRISDNET
EIDIPSTERPDEVGEIARAVVRFRDNTVALAQSRATLAEQARTLEITLANERRMAELQRNFVSMVSHEFRTPLTVIDGYA
QRLSHIKPPLSLEAVQDRSSRIRAAVRRMTALIEDMLGASQVLDLATPGAFQRTEFSLHLLVHEVCEMHRESSPDAQIVE
QWHADVPEVFHGDARLLFQAVSNLIGNAVKYSPPKTPVSVDVSMESGAILICVADQGIGIPEKDLGHVFERYVRGANVTG
TAGAGVGLYIVRLAAELHGGTVSVRSAEGTGSRFLLRLPEAPAPAPLSA
>Mature_528_residues
GRLSVAPLAQLNRLRVRLSLVFVLFLLLVIGLGAFSINRLSAFHNVAAEISGRWLLSNRIAGDLNNSISDFRATEGELLT
ARRGKDRGRAEGSLAVLDEEIADAKRRYETIEHDPIELDLYRQFSEQWRAYRELANRVLVLSQRDDKTDAIALYYQESRR
AFTVANATLATLTERNVTGANEATQAEAVAYRHARDLIVNAIIVAALLMIAGVLYFVTAVTNPIAELVDRMHRISDNETE
IDIPSTERPDEVGEIARAVVRFRDNTVALAQSRATLAEQARTLEITLANERRMAELQRNFVSMVSHEFRTPLTVIDGYAQ
RLSHIKPPLSLEAVQDRSSRIRAAVRRMTALIEDMLGASQVLDLATPGAFQRTEFSLHLLVHEVCEMHRESSPDAQIVEQ
WHADVPEVFHGDARLLFQAVSNLIGNAVKYSPPKTPVSVDVSMESGAILICVADQGIGIPEKDLGHVFERYVRGANVTGT
AGAGVGLYIVRLAAELHGGTVSVRSAEGTGSRFLLRLPEAPAPAPLSA

Specific function: Part of the two-component regulatory system senX3/regX3. Phosphorylates regX3 under conditions of phosphate limitation. Probably does not itself sense phosphate concentrations, which may be relayed to senX3 by the pstSCAB phosphate transporter system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87081816, Length=422, Percent_Identity=27.7251184834123, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1786912, Length=268, Percent_Identity=29.8507462686567, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786600, Length=227, Percent_Identity=29.5154185022026, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789149, Length=261, Percent_Identity=29.5019157088123, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI145693157, Length=281, Percent_Identity=27.4021352313167, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1790861, Length=346, Percent_Identity=24.5664739884393, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790436, Length=217, Percent_Identity=29.9539170506912, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1788393, Length=309, Percent_Identity=23.6245954692557, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790551, Length=337, Percent_Identity=27.299703264095, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI48994928, Length=230, Percent_Identity=29.5652173913043, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790346, Length=259, Percent_Identity=27.027027027027, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1786783, Length=226, Percent_Identity=28.3185840707965, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788549, Length=241, Percent_Identity=25.7261410788382, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1788713, Length=233, Percent_Identity=24.4635193133047, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 58265; Mature: 58134

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRLSVAPLAQLNRLRVRLSLVFVLFLLLVIGLGAFSINRLSAFHNVAAEISGRWLLSNR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEECCC
IAGDLNNSISDFRATEGELLTARRGKDRGRAEGSLAVLDEEIADAKRRYETIEHDPIELD
CCCCCCCCHHHHHCCCCCEEEHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
LYRQFSEQWRAYRELANRVLVLSQRDDKTDAIALYYQESRRAFTVANATLATLTERNVTG
HHHHHHHHHHHHHHHHHHEEEEECCCCCCCEEEEEEECCCCEEEEECHHHHHHHHCCCCC
ANEATQAEAVAYRHARDLIVNAIIVAALLMIAGVLYFVTAVTNPIAELVDRMHRISDNET
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EIDIPSTERPDEVGEIARAVVRFRDNTVALAQSRATLAEQARTLEITLANERRMAELQRN
EECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHH
FVSMVSHEFRTPLTVIDGYAQRLSHIKPPLSLEAVQDRSSRIRAAVRRMTALIEDMLGAS
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
QVLDLATPGAFQRTEFSLHLLVHEVCEMHRESSPDAQIVEQWHADVPEVFHGDARLLFQA
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHCCHHHHHHHH
VSNLIGNAVKYSPPKTPVSVDVSMESGAILICVADQGIGIPEKDLGHVFERYVRGANVTG
HHHHHCCCEECCCCCCCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCC
TAGAGVGLYIVRLAAELHGGTVSVRSAEGTGSRFLLRLPEAPAPAPLSA
CCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
GRLSVAPLAQLNRLRVRLSLVFVLFLLLVIGLGAFSINRLSAFHNVAAEISGRWLLSNR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEECCC
IAGDLNNSISDFRATEGELLTARRGKDRGRAEGSLAVLDEEIADAKRRYETIEHDPIELD
CCCCCCCCHHHHHCCCCCEEEHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
LYRQFSEQWRAYRELANRVLVLSQRDDKTDAIALYYQESRRAFTVANATLATLTERNVTG
HHHHHHHHHHHHHHHHHHEEEEECCCCCCCEEEEEEECCCCEEEEECHHHHHHHHCCCCC
ANEATQAEAVAYRHARDLIVNAIIVAALLMIAGVLYFVTAVTNPIAELVDRMHRISDNET
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EIDIPSTERPDEVGEIARAVVRFRDNTVALAQSRATLAEQARTLEITLANERRMAELQRN
EECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHH
FVSMVSHEFRTPLTVIDGYAQRLSHIKPPLSLEAVQDRSSRIRAAVRRMTALIEDMLGAS
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
QVLDLATPGAFQRTEFSLHLLVHEVCEMHRESSPDAQIVEQWHADVPEVFHGDARLLFQA
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHCCHHHHHHHH
VSNLIGNAVKYSPPKTPVSVDVSMESGAILICVADQGIGIPEKDLGHVFERYVRGANVTG
HHHHHCCCEECCCCCCCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCC
TAGAGVGLYIVRLAAELHGGTVSVRSAEGTGSRFLLRLPEAPAPAPLSA
CCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA