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The map label for this gene is alkB2 [H]

Identifier: 94314187

GI number: 94314187

Start: 1967008

End: 1967679

Strand: Direct

Name: alkB2 [H]

Synonym: Rmet_5268

Alternate gene names: 94314187

Gene position: 1967008-1967679 (Clockwise)

Preceding gene: 94314186

Following gene: 94314188

Centisome position: 76.24

GC content: 67.56

Gene sequence:

>672_bases
ATGCCCGCCATGACCTTCGACCTCTTCGACGCCCTTCCCGCCGATTCCAACGCCCCGCCTGCCGTGGAGACGCTTGCGCC
GGGCGCCGTGGTGTTGCATGGATTCGCGCGGGATGCGGGGCCGGAACTGCTCGCCGCCGTGCAGACGATCAGTGCCGAGG
CGCCGTTCCGCCATCTGATCACGCCGGGCGGCCTGCGCATGTCGGTGGCCATGACCAATTGCGGCGAGCGTGGCTGGGTG
TCCGATCGCACCGGGTATCGCTACGATGCGGTCGATCCGGAGTCAGGACGGCCGTGGCCGGCCATGCCTGCCGTGTTCCG
GTCGTTGGCCGAACACGCGGCGGAAGCGGCTGGCTATCCCGGTTTTGCACCCGATGCCTGCCTGATCAACCGCTATCTGC
CGGGCACGCGCCTGTCGCTGCATCAGGATCGCGACGAACTCGATCTGCGCGCACCGATCGTTTCTGTCTCAATGGGCCTG
CCGGCCGTCTTCCTCTGGGGTGGATTGCGTCGCGCCGACCGGCCGGGCCGATTCCGGCTCGCGCATGGTGACGTGGTGGT
CTGGGGTGGACCATCCAGGCTGGTCTTCCACGGCATCGCCCCGCTGGCCGACGGCGATCATGCGTTGACCGGAAGCGAGC
GAATCAACCTGACGTTTCGCAAGACCCGCTAA

Upstream 100 bases:

>100_bases
CAAGGCATTGCTGGAGGGGTTGGCGAAGGTGGGTGGCAAGTAGGCGTGACGGGCGGGTGAACGGCAACTGCGATCCCGGG
TACCATCCCAGGTACCCGAA

Downstream 100 bases:

>100_bases
GCAAGACCGCCAAGCAAGACCCAAGACCCGCCAATGACGCGGGAGTGCATGGGCAGTTGCCTGCACAGTAGGACAACCAC
TACGATAGCGGCACCGCACC

Product: alpha-ketoglutarate-dependent dioxygenase alkB

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MPAMTFDLFDALPADSNAPPAVETLAPGAVVLHGFARDAGPELLAAVQTISAEAPFRHLITPGGLRMSVAMTNCGERGWV
SDRTGYRYDAVDPESGRPWPAMPAVFRSLAEHAAEAAGYPGFAPDACLINRYLPGTRLSLHQDRDELDLRAPIVSVSMGL
PAVFLWGGLRRADRPGRFRLAHGDVVVWGGPSRLVFHGIAPLADGDHALTGSERINLTFRKTR

Sequences:

>Translated_223_residues
MPAMTFDLFDALPADSNAPPAVETLAPGAVVLHGFARDAGPELLAAVQTISAEAPFRHLITPGGLRMSVAMTNCGERGWV
SDRTGYRYDAVDPESGRPWPAMPAVFRSLAEHAAEAAGYPGFAPDACLINRYLPGTRLSLHQDRDELDLRAPIVSVSMGL
PAVFLWGGLRRADRPGRFRLAHGDVVVWGGPSRLVFHGIAPLADGDHALTGSERINLTFRKTR
>Mature_222_residues
PAMTFDLFDALPADSNAPPAVETLAPGAVVLHGFARDAGPELLAAVQTISAEAPFRHLITPGGLRMSVAMTNCGERGWVS
DRTGYRYDAVDPESGRPWPAMPAVFRSLAEHAAEAAGYPGFAPDACLINRYLPGTRLSLHQDRDELDLRAPIVSVSMGLP
AVFLWGGLRRADRPGRFRLAHGDVVVWGGPSRLVFHGIAPLADGDHALTGSERINLTFRKTR

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=121, Percent_Identity=33.8842975206612, Blast_Score=74, Evalue=8e-14,
Organism=Escherichia coli, GI1788541, Length=206, Percent_Identity=58.7378640776699, Blast_Score=255, Evalue=1e-69,
Organism=Drosophila melanogaster, GI45555401, Length=98, Percent_Identity=38.7755102040816, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004574 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 23989; Mature: 23857

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAMTFDLFDALPADSNAPPAVETLAPGAVVLHGFARDAGPELLAAVQTISAEAPFRHLI
CCCCCHHHHHCCCCCCCCCCCHHHHCCCCEEEEEHHCCCCHHHHHHHHHHCCCCCHHHHC
TPGGLRMSVAMTNCGERGWVSDRTGYRYDAVDPESGRPWPAMPAVFRSLAEHAAEAAGYP
CCCCCEEEEEECCCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
GFAPDACLINRYLPGTRLSLHQDRDELDLRAPIVSVSMGLPAVFLWGGLRRADRPGRFRL
CCCCCHHHHHHCCCCCEEEECCCCCHHHHHCCEEEECCCCCCEEHHCCHHCCCCCCCEEE
AHGDVVVWGGPSRLVFHGIAPLADGDHALTGSERINLTFRKTR
EECCEEEECCCCEEEEECCCCCCCCCCEECCCCEEEEEEEECC
>Mature Secondary Structure 
PAMTFDLFDALPADSNAPPAVETLAPGAVVLHGFARDAGPELLAAVQTISAEAPFRHLI
CCCCHHHHHCCCCCCCCCCCHHHHCCCCEEEEEHHCCCCHHHHHHHHHHCCCCCHHHHC
TPGGLRMSVAMTNCGERGWVSDRTGYRYDAVDPESGRPWPAMPAVFRSLAEHAAEAAGYP
CCCCCEEEEEECCCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
GFAPDACLINRYLPGTRLSLHQDRDELDLRAPIVSVSMGLPAVFLWGGLRRADRPGRFRL
CCCCCHHHHHHCCCCCEEEECCCCCHHHHHCCEEEECCCCCCEEHHCCHHCCCCCCCEEE
AHGDVVVWGGPSRLVFHGIAPLADGDHALTGSERINLTFRKTR
EECCEEEECCCCEEEEECCCCCCCCCCEECCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536913; 9097040; 9278503; 3887409; 7928996 [H]