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The map label for this gene is pcaQ [H]

Identifier: 94314166

GI number: 94314166

Start: 1945549

End: 1946520

Strand: Reverse

Name: pcaQ [H]

Synonym: Rmet_5247

Alternate gene names: 94314166

Gene position: 1946520-1945549 (Counterclockwise)

Preceding gene: 94314169

Following gene: 94314164

Centisome position: 75.44

GC content: 66.15

Gene sequence:

>972_bases
ATGACGCGGGTATCGCCTGAGCAACACGGTATGGGCCTCCACAGGCCAGCGCGCGCGCAGCGGCTGGATCGCTTGCGCAT
CCGGCATCTACGCTTTCTGGAACTCGTGGCCGAGCATGGCTCGTTGACGACGGCGGCCGCACAACTGGCGCTCAGCCAGC
CTGCCGCAACCAAGATGCTGCAGGAGCTGGAATCCGCGTTCGGGGTGGAACTGGTCGCGCGTACCACGCGCGGCGCGGCA
CTATCCCCTGCGGGACTGGTTGCGCTGGACCGCCTGCGTATCGCGCTCAGCGCGCTGGCGGTGGCGGCTCAGTCAACGAC
GGAAACACCCCCCGCTCCCGTGGTGAGGCTTGGCGTACTCCCGCTGGTTGGCGTGGTGATCCTGCCGAGATTGCTGGCGG
CAATGGCTGCGCGGCCAGACTGCCCAAGACTCGTCGTGCGTGAATCGACGGTGGGCGGGCTGCTTCGAATGTTGAGCCGT
GGCGATGTCGACTGCGTGATTGGCAGAATCGGCGTGGAGCTGGATGAGCAGGCCGCCAGCAGGCTGGCGGTGACGCCGCT
GTGGCAGGAGCCGCTCGCCATTGCGTGCGCACAGGACCACCCACTGCGCCGCAGGCGCAACGTCGGGTTGGCGGAACTGC
GCGAGCTGGGTTGGATCGTGGCGTCACGCGAGGCCTATACGCGCCAGGTGTTCGACGACACCTTCCTGTCCGCAGGCCTG
GTGCCGCCCCATCCCAGGGTCGAGTCGTTCTCGTTCCACACGAATCTGAGTGTGGTGGCAAGCAGCGATCTCTTGACCGT
CGCACCGGAAAGCGCCGTGGCGCACTATGCGCAACTGGGAATGGTGCACCCGGTACGCCATGATTTTTCGTTCCCGGTGG
GGCGCACGGTATTCATCACGATGGCGGAGATGGGGGCCTGGCCCGGCATTACGCTGATCCGGGATGCCTTGGCTGATCTG
ACTGTCCCCTGA

Upstream 100 bases:

>100_bases
TTGCTCTGGTCTTGCTCTAGTGAGCTTGTTCGACAGCATTGTGCGAGCCGGATTCTTTCCTGTAAATTATGATGTCTTGA
TAGCATCATCACGATTTGTT

Downstream 100 bases:

>100_bases
GATTTCGCTCGCTCAATGCGCTGCCCGTGGCCCGCGTCGGCTGCCATTGCATGACGCAGATGCCCGCCAGGACCAGCACC
ACGCCGGCCAGGCGCGCCAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Pca operon transcriptional activator [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MTRVSPEQHGMGLHRPARAQRLDRLRIRHLRFLELVAEHGSLTTAAAQLALSQPAATKMLQELESAFGVELVARTTRGAA
LSPAGLVALDRLRIALSALAVAAQSTTETPPAPVVRLGVLPLVGVVILPRLLAAMAARPDCPRLVVRESTVGGLLRMLSR
GDVDCVIGRIGVELDEQAASRLAVTPLWQEPLAIACAQDHPLRRRRNVGLAELRELGWIVASREAYTRQVFDDTFLSAGL
VPPHPRVESFSFHTNLSVVASSDLLTVAPESAVAHYAQLGMVHPVRHDFSFPVGRTVFITMAEMGAWPGITLIRDALADL
TVP

Sequences:

>Translated_323_residues
MTRVSPEQHGMGLHRPARAQRLDRLRIRHLRFLELVAEHGSLTTAAAQLALSQPAATKMLQELESAFGVELVARTTRGAA
LSPAGLVALDRLRIALSALAVAAQSTTETPPAPVVRLGVLPLVGVVILPRLLAAMAARPDCPRLVVRESTVGGLLRMLSR
GDVDCVIGRIGVELDEQAASRLAVTPLWQEPLAIACAQDHPLRRRRNVGLAELRELGWIVASREAYTRQVFDDTFLSAGL
VPPHPRVESFSFHTNLSVVASSDLLTVAPESAVAHYAQLGMVHPVRHDFSFPVGRTVFITMAEMGAWPGITLIRDALADL
TVP
>Mature_322_residues
TRVSPEQHGMGLHRPARAQRLDRLRIRHLRFLELVAEHGSLTTAAAQLALSQPAATKMLQELESAFGVELVARTTRGAAL
SPAGLVALDRLRIALSALAVAAQSTTETPPAPVVRLGVLPLVGVVILPRLLAAMAARPDCPRLVVRESTVGGLLRMLSRG
DVDCVIGRIGVELDEQAASRLAVTPLWQEPLAIACAQDHPLRRRRNVGLAELRELGWIVASREAYTRQVFDDTFLSAGLV
PPHPRVESFSFHTNLSVVASSDLLTVAPESAVAHYAQLGMVHPVRHDFSFPVGRTVFITMAEMGAWPGITLIRDALADLT
VP

Specific function: Activates transcription of the pcaDCHGB operon for the catabolism of the phenolic compound protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=246, Percent_Identity=30.4878048780488, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87081904, Length=253, Percent_Identity=30.8300395256917, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790399, Length=192, Percent_Identity=30.7291666666667, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1789506, Length=279, Percent_Identity=23.6559139784946, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787601, Length=250, Percent_Identity=22.8, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR012787
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34889; Mature: 34758

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRVSPEQHGMGLHRPARAQRLDRLRIRHLRFLELVAEHGSLTTAAAQLALSQPAATKML
CCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHH
QELESAFGVELVARTTRGAALSPAGLVALDRLRIALSALAVAAQSTTETPPAPVVRLGVL
HHHHHHHCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
PLVGVVILPRLLAAMAARPDCPRLVVRESTVGGLLRMLSRGDVDCVIGRIGVELDEQAAS
HHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHCCCHHEEHHHHCCCCCHHHHH
RLAVTPLWQEPLAIACAQDHPLRRRRNVGLAELRELGWIVASREAYTRQVFDDTFLSAGL
HEECCCCCCCCCEEEECCCCCHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
VPPHPRVESFSFHTNLSVVASSDLLTVAPESAVAHYAQLGMVHPVRHDFSFPVGRTVFIT
CCCCCCCCCEEEECCEEEEECCCEEEECCHHHHHHHHHHCCCCCHHCCCCCCCCCEEEEE
MAEMGAWPGITLIRDALADLTVP
EHHHCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
TRVSPEQHGMGLHRPARAQRLDRLRIRHLRFLELVAEHGSLTTAAAQLALSQPAATKML
CCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHH
QELESAFGVELVARTTRGAALSPAGLVALDRLRIALSALAVAAQSTTETPPAPVVRLGVL
HHHHHHHCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
PLVGVVILPRLLAAMAARPDCPRLVVRESTVGGLLRMLSRGDVDCVIGRIGVELDEQAAS
HHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHCCCHHEEHHHHCCCCCHHHHH
RLAVTPLWQEPLAIACAQDHPLRRRRNVGLAELRELGWIVASREAYTRQVFDDTFLSAGL
HEECCCCCCCCCEEEECCCCCHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
VPPHPRVESFSFHTNLSVVASSDLLTVAPESAVAHYAQLGMVHPVRHDFSFPVGRTVFIT
CCCCCCCCCEEEECCEEEEECCCEEEECCHHHHHHHHHHCCCCCHHCCCCCCCCCEEEEE
MAEMGAWPGITLIRDALADLTVP
EHHHCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]