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The map label for this gene is iscS2 [H]

Identifier: 94314149

GI number: 94314149

Start: 1926592

End: 1928097

Strand: Reverse

Name: iscS2 [H]

Synonym: Rmet_5230

Alternate gene names: 94314149

Gene position: 1928097-1926592 (Counterclockwise)

Preceding gene: 94314163

Following gene: 94314147

Centisome position: 74.73

GC content: 61.42

Gene sequence:

>1506_bases
ATGAGCACGGACACTCTGCGGTACTTCGATTACGCTGCAACCACACCGGCGGACCCTCGTGTCATGGAGGCGATGTTCGG
CTGCCTGGGGGCCGCCGGCGTCTTCGGAAACCCTGCGTCGAACTCCCACGCTGCAGGGCTGAAGGCAAAGGATCTGGTCG
AGCAGGCTCGCGCCCAGGTCGCCGCTCTGATTGGTGCAGAGCCATCCGAGATTGTCTGGACGTCCGGCGCGACAGAGTCG
AACAATCTGGCTCTCAAGGGCTTTTTCGACCGCGAGCCGACACGGCGCCATTTGGTTACCAGCAAGCTTGAGCACAAGGC
AATTCTGGACACCGCGTCGTACCTTGAGCGACTTGACGTTTCCGTTTCCTACCTCTTGCCGGATCCGTCAGGCGAGATTT
CGCAGGCCGCTGTGGAAGCGGCCTGCAGCCATCACACGGGCCTCGTTTCGTTGATGATGGTGAACAACGAGATCGGCACC
CTTACCAACATCGCCGAGATCTCGCGGCTCGTTCACGCCGCTGGCGCTCTGCTTCACGTGGACGCGGCGCAGGCGGTTGG
CAAGACCGCGATTGACGTAAAGGCGATGGGCGTGGATCTGCTGTCGATGTCGGCACACAAAGTATATGGTCCGAAGGGCA
TCGGTGCGCTCTATGTGAGCCGCGAAGTGGCATCGCGTATCGCTTGCCAGATGCATGGTGGAGGACACGAGCAGGGACTA
CGGTCTGGCACCCTCGCCACGCACCAGATTGTCGGCATGGGAACGGCTTGCGAACTGGCGCGGACAGAAATGGCTTCGGA
CAACGAGCGCATCGCGCAACTGAGCGAGCGTTTGCTGAGTGGTGTGCGCGCGACAGGCGCCATCACGCAAAACGTCAGTT
CCGCAGTCCGCATTCCGCACACACTCAGTCTGACTGTGGAGTTGCCCAATTTTCTCCCGTCCATGTTGGCCGACAACCTG
GCCATCTCCTCAACGTCTGCCTGCAATTCGGCGGCTGGCAAACCGTCTCACGTGCTGACTTGCATCGGTCTGGGTAAGGA
TTCGGCGGAGCGCACAGTTCGCCTGAGCCTCGGACGCTTCACAACGGAGGCAGACGTGGATTTTGCGGTCGACTGCTTTC
GTGGGGCCATAGAGCGCTGTCTGGAAGTGGGCGCGGGCCGACAGGACATGCAGATCAGGCAGCTCTCGAACGACGTGAGC
GTGGCGCCACAGTTAAGCGGAGCCGACGTACGGGCGGCGCATGCGATTGGATTCCGTTCCATTGTCTGCAACCGGCACGA
TGGAGAGGCGGCCGGCCAGACCCAGTTCGCGAAAATCGCGGAGGTTGCCAGTTCGCTTGGCATGGCTGCGCACTACCTGC
CCGTGGAATCGGGTGATGTCACCGTCGAAGCCGCTGCAGCCTTCGGTTTGCTCGTGGCAGAGCTGCCGAAGCCCGTACTT
GCGTACTGCCGTACGGGAACCCGTTCGGCTACGCTGTGGAATTTGGCAAGCAGCAGGCAAGGCTGA

Upstream 100 bases:

>100_bases
GGTAGCATCATATGCTCCACATTCCAATATCGATCATGGATTTTGGAAGCAATTTTTAGTGCGCTCGTCGTAGCATTTCT
ACCCTTCTGAAGGATGAATG

Downstream 100 bases:

>100_bases
AGGGGCGGTCGGCACGGAAAGATTGCGCGACGGGGCGTGCGTGGGTTGACGTGCCGTCCTCGGCGTTCGGCCAACGATCC
TGCGGAAGGTAAGCAAAAAC

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MSTDTLRYFDYAATTPADPRVMEAMFGCLGAAGVFGNPASNSHAAGLKAKDLVEQARAQVAALIGAEPSEIVWTSGATES
NNLALKGFFDREPTRRHLVTSKLEHKAILDTASYLERLDVSVSYLLPDPSGEISQAAVEAACSHHTGLVSLMMVNNEIGT
LTNIAEISRLVHAAGALLHVDAAQAVGKTAIDVKAMGVDLLSMSAHKVYGPKGIGALYVSREVASRIACQMHGGGHEQGL
RSGTLATHQIVGMGTACELARTEMASDNERIAQLSERLLSGVRATGAITQNVSSAVRIPHTLSLTVELPNFLPSMLADNL
AISSTSACNSAAGKPSHVLTCIGLGKDSAERTVRLSLGRFTTEADVDFAVDCFRGAIERCLEVGAGRQDMQIRQLSNDVS
VAPQLSGADVRAAHAIGFRSIVCNRHDGEAAGQTQFAKIAEVASSLGMAAHYLPVESGDVTVEAAAAFGLLVAELPKPVL
AYCRTGTRSATLWNLASSRQG

Sequences:

>Translated_501_residues
MSTDTLRYFDYAATTPADPRVMEAMFGCLGAAGVFGNPASNSHAAGLKAKDLVEQARAQVAALIGAEPSEIVWTSGATES
NNLALKGFFDREPTRRHLVTSKLEHKAILDTASYLERLDVSVSYLLPDPSGEISQAAVEAACSHHTGLVSLMMVNNEIGT
LTNIAEISRLVHAAGALLHVDAAQAVGKTAIDVKAMGVDLLSMSAHKVYGPKGIGALYVSREVASRIACQMHGGGHEQGL
RSGTLATHQIVGMGTACELARTEMASDNERIAQLSERLLSGVRATGAITQNVSSAVRIPHTLSLTVELPNFLPSMLADNL
AISSTSACNSAAGKPSHVLTCIGLGKDSAERTVRLSLGRFTTEADVDFAVDCFRGAIERCLEVGAGRQDMQIRQLSNDVS
VAPQLSGADVRAAHAIGFRSIVCNRHDGEAAGQTQFAKIAEVASSLGMAAHYLPVESGDVTVEAAAAFGLLVAELPKPVL
AYCRTGTRSATLWNLASSRQG
>Mature_500_residues
STDTLRYFDYAATTPADPRVMEAMFGCLGAAGVFGNPASNSHAAGLKAKDLVEQARAQVAALIGAEPSEIVWTSGATESN
NLALKGFFDREPTRRHLVTSKLEHKAILDTASYLERLDVSVSYLLPDPSGEISQAAVEAACSHHTGLVSLMMVNNEIGTL
TNIAEISRLVHAAGALLHVDAAQAVGKTAIDVKAMGVDLLSMSAHKVYGPKGIGALYVSREVASRIACQMHGGGHEQGLR
SGTLATHQIVGMGTACELARTEMASDNERIAQLSERLLSGVRATGAITQNVSSAVRIPHTLSLTVELPNFLPSMLADNLA
ISSTSACNSAAGKPSHVLTCIGLGKDSAERTVRLSLGRFTTEADVDFAVDCFRGAIERCLEVGAGRQDMQIRQLSNDVSV
APQLSGADVRAAHAIGFRSIVCNRHDGEAAGQTQFAKIAEVASSLGMAAHYLPVESGDVTVEAAAAFGLLVAELPKPVLA
YCRTGTRSATLWNLASSRQG

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=381, Percent_Identity=45.1443569553806, Blast_Score=316, Evalue=3e-86,
Organism=Homo sapiens, GI156713448, Length=411, Percent_Identity=33.0900243309002, Blast_Score=190, Evalue=3e-48,
Organism=Escherichia coli, GI48994898, Length=383, Percent_Identity=48.3028720626632, Blast_Score=357, Evalue=1e-99,
Organism=Escherichia coli, GI1787970, Length=273, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1789175, Length=277, Percent_Identity=27.7978339350181, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25143064, Length=374, Percent_Identity=44.9197860962567, Blast_Score=302, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI17533177, Length=317, Percent_Identity=30.2839116719243, Blast_Score=126, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6319831, Length=369, Percent_Identity=43.0894308943089, Blast_Score=289, Evalue=7e-79,
Organism=Drosophila melanogaster, GI20129463, Length=383, Percent_Identity=43.8642297650131, Blast_Score=292, Evalue=3e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 52574; Mature: 52443

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDTLRYFDYAATTPADPRVMEAMFGCLGAAGVFGNPASNSHAAGLKAKDLVEQARAQV
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
AALIGAEPSEIVWTSGATESNNLALKGFFDREPTRRHLVTSKLEHKAILDTASYLERLDV
HHHHCCCCCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
SVSYLLPDPSGEISQAAVEAACSHHTGLVSLMMVNNEIGTLTNIAEISRLVHAAGALLHV
EEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEE
DAAQAVGKTAIDVKAMGVDLLSMSAHKVYGPKGIGALYVSREVASRIACQMHGGGHEQGL
HHHHHHCCHHEEEEECCHHHHHHCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
RSGTLATHQIVGMGTACELARTEMASDNERIAQLSERLLSGVRATGAITQNVSSAVRIPH
CCCCCHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCE
TLSLTVELPNFLPSMLADNLAISSTSACNSAAGKPSHVLTCIGLGKDSAERTVRLSLGRF
EEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCC
TTEADVDFAVDCFRGAIERCLEVGAGRQDMQIRQLSNDVSVAPQLSGADVRAAHAIGFRS
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
IVCNRHDGEAAGQTQFAKIAEVASSLGMAAHYLPVESGDVTVEAAAAFGLLVAELPKPVL
HEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEHHHHHHHHHHHCCHHHH
AYCRTGTRSATLWNLASSRQG
HHHCCCCCCCEEEECHHCCCC
>Mature Secondary Structure 
STDTLRYFDYAATTPADPRVMEAMFGCLGAAGVFGNPASNSHAAGLKAKDLVEQARAQV
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
AALIGAEPSEIVWTSGATESNNLALKGFFDREPTRRHLVTSKLEHKAILDTASYLERLDV
HHHHCCCCCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
SVSYLLPDPSGEISQAAVEAACSHHTGLVSLMMVNNEIGTLTNIAEISRLVHAAGALLHV
EEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEE
DAAQAVGKTAIDVKAMGVDLLSMSAHKVYGPKGIGALYVSREVASRIACQMHGGGHEQGL
HHHHHHCCHHEEEEECCHHHHHHCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
RSGTLATHQIVGMGTACELARTEMASDNERIAQLSERLLSGVRATGAITQNVSSAVRIPH
CCCCCHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCE
TLSLTVELPNFLPSMLADNLAISSTSACNSAAGKPSHVLTCIGLGKDSAERTVRLSLGRF
EEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCC
TTEADVDFAVDCFRGAIERCLEVGAGRQDMQIRQLSNDVSVAPQLSGADVRAAHAIGFRS
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
IVCNRHDGEAAGQTQFAKIAEVASSLGMAAHYLPVESGDVTVEAAAAFGLLVAELPKPVL
HEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEHHHHHHHHHHHCCHHHH
AYCRTGTRSATLWNLASSRQG
HHHCCCCCCCEEEECHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA