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The map label for this gene is purR [H]

Identifier: 94313872

GI number: 94313872

Start: 1615313

End: 1616338

Strand: Direct

Name: purR [H]

Synonym: Rmet_4950

Alternate gene names: 94313872

Gene position: 1615313-1616338 (Clockwise)

Preceding gene: 94313866

Following gene: 94313877

Centisome position: 62.61

GC content: 66.96

Gene sequence:

>1026_bases
ATGGCAGAAAAGCAGATCAGCATCCGTGACGTCGCTGCCCTGGCCGGTGTATCGGTCGGTAGCGCGTCGCGCGTGATCAA
CGGCGCCGCCAACGTCACGCCGGACGTGCGCGAGCGCGTCGATCGCGCCATCGCGGCACTGCGGTATCGCCCCAACCACG
CGGCGCAATCGCTGCGTTCGCGCACGAGCAAGACCATCGGATGCATGTTTTCGGACGTGGCCAACCCGCTCTACGCACGC
GCCTTCCGCGCGCTCGAGGAACATTTCCGGCGTGAGGGATACATGCTGCTGCTGGCGAACGGCCTCAACGATGCCGAACG
CGAGATCGACATCATCCAGACGTTCCAGTTACGCGGCATGGACGGCGTTATCCTGGCGCCGGGCAACGAGCGACATCCGG
GCGTGCTCGATGCGGTGCGCCGGCTGTCGATGCCGGCCGTAATCTACGACCGCGACTTGGCAGTGGATGCCGATGCCGTG
CTGTTCGACCACGTGGCCGGCGTGAAGGCCGCGATCCATCATCTGGTGGAACTCGGCCATACGCGCATCGCGCTGGCGCT
ATGGCAGGTCAAGAGCCGCCCGGTGCGCCGGCGGATCGAAGGCTACAAGGCCGCCTTCAAGGCCGCCGGCCTCCCGGTAC
CGGACCTGATCCTGCAGGAACCCACACCCACCAGCTCCGCTTTCGACGACGTCACGCGGCTGCTGCAACTGCCTGACCCG
CCCACGGCATTCCTTGCCCAGGGCACCCACACGCTCACCAGCACGCTGCGCGCGATCGCCAAGCAAGGCCTGCGCGTGCC
GGAGGACGTCTCGGTGATCGGTATCGGCGATACCGACTTCGCGCAGACCTACGATCCGCCGCTGACCGTGCTGCGCACAC
CAACGGACGAGGTAGCCGCACACGCCGTGGAGATGCTGCTTTCACGCCTGGCCGCCAAGGGGGACGCCAGCCTGTCGCCA
CGCAAGGCGCTGATCCAGTTCGACCTGATCCTGCGCGAGTCGTGCGCGCCCGTTGCCCGGCACTGA

Upstream 100 bases:

>100_bases
CAGCAATGAAACGTTTCATCAAGCGGAGTTTTGCCATTTCCCTCCGCGACCGAAGCACTCTGGCCGTCGGTTATACTCTT
TGGCAACTTCAGGGAATCCC

Downstream 100 bases:

>100_bases
ATCGCCTCGCAGGAAAGCGATGACCGGTTAGTCGGCGCTGGCACCCGACGCCTTGACGATGGTCGCCCAGCGCGCGATTT
CCTTCTGCACGAACGTCCTG

Product: putative LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MAEKQISIRDVAALAGVSVGSASRVINGAANVTPDVRERVDRAIAALRYRPNHAAQSLRSRTSKTIGCMFSDVANPLYAR
AFRALEEHFRREGYMLLLANGLNDAEREIDIIQTFQLRGMDGVILAPGNERHPGVLDAVRRLSMPAVIYDRDLAVDADAV
LFDHVAGVKAAIHHLVELGHTRIALALWQVKSRPVRRRIEGYKAAFKAAGLPVPDLILQEPTPTSSAFDDVTRLLQLPDP
PTAFLAQGTHTLTSTLRAIAKQGLRVPEDVSVIGIGDTDFAQTYDPPLTVLRTPTDEVAAHAVEMLLSRLAAKGDASLSP
RKALIQFDLILRESCAPVARH

Sequences:

>Translated_341_residues
MAEKQISIRDVAALAGVSVGSASRVINGAANVTPDVRERVDRAIAALRYRPNHAAQSLRSRTSKTIGCMFSDVANPLYAR
AFRALEEHFRREGYMLLLANGLNDAEREIDIIQTFQLRGMDGVILAPGNERHPGVLDAVRRLSMPAVIYDRDLAVDADAV
LFDHVAGVKAAIHHLVELGHTRIALALWQVKSRPVRRRIEGYKAAFKAAGLPVPDLILQEPTPTSSAFDDVTRLLQLPDP
PTAFLAQGTHTLTSTLRAIAKQGLRVPEDVSVIGIGDTDFAQTYDPPLTVLRTPTDEVAAHAVEMLLSRLAAKGDASLSP
RKALIQFDLILRESCAPVARH
>Mature_340_residues
AEKQISIRDVAALAGVSVGSASRVINGAANVTPDVRERVDRAIAALRYRPNHAAQSLRSRTSKTIGCMFSDVANPLYARA
FRALEEHFRREGYMLLLANGLNDAEREIDIIQTFQLRGMDGVILAPGNERHPGVLDAVRRLSMPAVIYDRDLAVDADAVL
FDHVAGVKAAIHHLVELGHTRIALALWQVKSRPVRRRIEGYKAAFKAAGLPVPDLILQEPTPTSSAFDDVTRLLQLPDPP
TAFLAQGTHTLTSTLRAIAKQGLRVPEDVSVIGIGDTDFAQTYDPPLTVLRTPTDEVAAHAVEMLLSRLAAKGDASLSPR
KALIQFDLILRESCAPVARH

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=306, Percent_Identity=32.0261437908497, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=29.1291291291291, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1790369, Length=335, Percent_Identity=26.865671641791, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1786540, Length=294, Percent_Identity=28.5714285714286, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788474, Length=319, Percent_Identity=28.8401253918495, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1789202, Length=291, Percent_Identity=28.8659793814433, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1789068, Length=336, Percent_Identity=29.7619047619048, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1787580, Length=328, Percent_Identity=26.8292682926829, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1787906, Length=325, Percent_Identity=28.3076923076923, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI48994940, Length=332, Percent_Identity=25, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790715, Length=323, Percent_Identity=23.5294117647059, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786268, Length=124, Percent_Identity=33.0645161290323, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37130; Mature: 36999

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKQISIRDVAALAGVSVGSASRVINGAANVTPDVRERVDRAIAALRYRPNHAAQSLRS
CCCCCCHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
RTSKTIGCMFSDVANPLYARAFRALEEHFRREGYMLLLANGLNDAEREIDIIQTFQLRGM
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHCCC
DGVILAPGNERHPGVLDAVRRLSMPAVIYDRDLAVDADAVLFDHVAGVKAAIHHLVELGH
CEEEECCCCCCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
TRIALALWQVKSRPVRRRIEGYKAAFKAAGLPVPDLILQEPTPTSSAFDDVTRLLQLPDP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCC
PTAFLAQGTHTLTSTLRAIAKQGLRVPEDVSVIGIGDTDFAQTYDPPLTVLRTPTDEVAA
CHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHCCCCCCEEEECCHHHHHH
HAVEMLLSRLAAKGDASLSPRKALIQFDLILRESCAPVARH
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AEKQISIRDVAALAGVSVGSASRVINGAANVTPDVRERVDRAIAALRYRPNHAAQSLRS
CCCCCHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
RTSKTIGCMFSDVANPLYARAFRALEEHFRREGYMLLLANGLNDAEREIDIIQTFQLRGM
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHCCC
DGVILAPGNERHPGVLDAVRRLSMPAVIYDRDLAVDADAVLFDHVAGVKAAIHHLVELGH
CEEEECCCCCCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
TRIALALWQVKSRPVRRRIEGYKAAFKAAGLPVPDLILQEPTPTSSAFDDVTRLLQLPDP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCC
PTAFLAQGTHTLTSTLRAIAKQGLRVPEDVSVIGIGDTDFAQTYDPPLTVLRTPTDEVAA
CHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHCCCCCCEEEECCHHHHHH
HAVEMLLSRLAAKGDASLSPRKALIQFDLILRESCAPVARH
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA