Click here to switch to the map view.

The map label for this gene is gst [H]

Identifier: 94313851

GI number: 94313851

Start: 1592549

End: 1593256

Strand: Direct

Name: gst [H]

Synonym: Rmet_4929

Alternate gene names: 94313851

Gene position: 1592549-1593256 (Clockwise)

Preceding gene: 94313850

Following gene: 94313853

Centisome position: 61.72

GC content: 61.72

Gene sequence:

>708_bases
ATGGCAGACCTGTCCGCCTTCCCCATTACCAAGCGATGGCCGGCGCAACATCCGGATCGCCTGCAGTTGTACTCGCTGAA
TACCCCCAACGGCATCAAGGTATCGTTGATGCTCGAGGAAACCGGCCTCCCCTACGAACCGCATTTGGTCCGCTTCGACA
AGAACGACCAGATGACGCCGGAATTCCTGTCACTGAATCCGAACAACAAGATTCCGGCCATCCTCGACCCCAATGGCCCC
GGTGGCAAGCCGCTGGCACTGTGGGAATCGGGCGCGATCCTGCTCTACCTGGCCGAAAAGACCGGCAAGTTCATGTCCGC
CGATCCGGCCCTGCGCTACGAGACGATCCAGTGGGTCATGTTCCAGATGGGCGGCATTGGCCCGATGTTCGGACAGGTTG
GCTTCTTCAACCGGTTCGCCGGCAAGGACTTCGAGGACAAGCGTCCGCGTGACCGCTATGTCAGCGAATCGCGCCGCCTG
CTGAACGTGCTGAACGAACGCCTGAAGGGGCGCCAATGGATCATGGGCGACGACTACACGATCGCCGACATGGCCACCTT
CCCGTGGATCCGCAACCTGCTGGGCTTCTACGAAGCCGGCGATCTGGTCGGCATTCAGGACTTCCCCGAAGTCACGCGCG
TGCTCGGTGCGTTCGTGGCCCGCCCGGCCGTGCAACGCGCGGTCAATATCCCGAAGCGCCCTGAATGA

Upstream 100 bases:

>100_bases
TAGCACGGCATACCGAAGCGCCCGAGTGACCGGGCGCTTCGGCAACCTTCCGCCGAAGCGTTTACCATCCTCGTCTAGCC
TTCAACGTTACGAGGATCTC

Downstream 100 bases:

>100_bases
TCCGGACTCATGCCGGATAAGGCGAATTACGTGGCGTATTACGCGGCGTAGCCCAGCAACGCGCTGATCGCCGCGGCTTC
TTCGCGCACGGCGCGGCCGA

Product: glutathione S-transferase

Products: NA

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MADLSAFPITKRWPAQHPDRLQLYSLNTPNGIKVSLMLEETGLPYEPHLVRFDKNDQMTPEFLSLNPNNKIPAILDPNGP
GGKPLALWESGAILLYLAEKTGKFMSADPALRYETIQWVMFQMGGIGPMFGQVGFFNRFAGKDFEDKRPRDRYVSESRRL
LNVLNERLKGRQWIMGDDYTIADMATFPWIRNLLGFYEAGDLVGIQDFPEVTRVLGAFVARPAVQRAVNIPKRPE

Sequences:

>Translated_235_residues
MADLSAFPITKRWPAQHPDRLQLYSLNTPNGIKVSLMLEETGLPYEPHLVRFDKNDQMTPEFLSLNPNNKIPAILDPNGP
GGKPLALWESGAILLYLAEKTGKFMSADPALRYETIQWVMFQMGGIGPMFGQVGFFNRFAGKDFEDKRPRDRYVSESRRL
LNVLNERLKGRQWIMGDDYTIADMATFPWIRNLLGFYEAGDLVGIQDFPEVTRVLGAFVARPAVQRAVNIPKRPE
>Mature_234_residues
ADLSAFPITKRWPAQHPDRLQLYSLNTPNGIKVSLMLEETGLPYEPHLVRFDKNDQMTPEFLSLNPNNKIPAILDPNGPG
GKPLALWESGAILLYLAEKTGKFMSADPALRYETIQWVMFQMGGIGPMFGQVGFFNRFAGKDFEDKRPRDRYVSESRRLL
NVLNERLKGRQWIMGDDYTIADMATFPWIRNLLGFYEAGDLVGIQDFPEVTRVLGAFVARPAVQRAVNIPKRPE

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=171, Percent_Identity=49.1228070175439, Blast_Score=159, Evalue=1e-40,
Organism=Escherichia coli, GI87082195, Length=221, Percent_Identity=40.7239819004525, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI87081789, Length=209, Percent_Identity=29.6650717703349, Blast_Score=66, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324100, Length=270, Percent_Identity=31.1111111111111, Blast_Score=111, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28571670, Length=181, Percent_Identity=31.4917127071823, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI17933730, Length=200, Percent_Identity=29, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI45549270, Length=188, Percent_Identity=29.2553191489362, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI17864592, Length=178, Percent_Identity=27.5280898876405, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: NA

Molecular weight: Translated: 26702; Mature: 26571

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLSAFPITKRWPAQHPDRLQLYSLNTPNGIKVSLMLEETGLPYEPHLVRFDKNDQMTP
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCC
EFLSLNPNNKIPAILDPNGPGGKPLALWESGAILLYLAEKTGKFMSADPALRYETIQWVM
CEEEECCCCCCCEEECCCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCHHHHHHHHHH
FQMGGIGPMFGQVGFFNRFAGKDFEDKRPRDRYVSESRRLLNVLNERLKGRQWIMGDDYT
HHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCEE
IADMATFPWIRNLLGFYEAGDLVGIQDFPEVTRVLGAFVARPAVQRAVNIPKRPE
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ADLSAFPITKRWPAQHPDRLQLYSLNTPNGIKVSLMLEETGLPYEPHLVRFDKNDQMTP
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCC
EFLSLNPNNKIPAILDPNGPGGKPLALWESGAILLYLAEKTGKFMSADPALRYETIQWVM
CEEEECCCCCCCEEECCCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCHHHHHHHHHH
FQMGGIGPMFGQVGFFNRFAGKDFEDKRPRDRYVSESRRLLNVLNERLKGRQWIMGDDYT
HHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCEE
IADMATFPWIRNLLGFYEAGDLVGIQDFPEVTRVLGAFVARPAVQRAVNIPKRPE
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]