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The map label for this gene is benC [H]

Identifier: 94313806

GI number: 94313806

Start: 1546035

End: 1547054

Strand: Direct

Name: benC [H]

Synonym: Rmet_4884

Alternate gene names: 94313806

Gene position: 1546035-1547054 (Clockwise)

Preceding gene: 94313805

Following gene: 94313807

Centisome position: 59.92

GC content: 67.06

Gene sequence:

>1020_bases
ATGGAACATACGATCGCCCTGCAGTTCGAAGATGGCGTGACGCGCTTCATCACGTGCGGCGACAACGAGACGCTCTCGGA
CGCCGCCTATCGCCAGCAGATCAACATTCCGCTCGACTGCCGCGACGGCGCGTGCGGCACCTGTCGCGGCCTATGCGAAT
CCGGCCACTATGACCTGCCCGAATCGAGCTATATCGAGGACGCGCTGACCGCCGAGGAGGCGGCCAAGGGATACGTGCTG
GCCTGCAAGACACGGCCGCGCTCGGATTGCGTGATCCGCGTGCCGGCTTCGTCCGCGGCCTGCAAGACTGGCGTGACGCG
ATTCGAGGGCGCGATCGCCGCCGTGGCGCCGCTGTCGGATTCGACCATCGGCTTCTCGATCGACCTCGATGACCCGGCCG
CGCTCAGCTTCCTGCCCGGGCAGTACGTCAACGTGGAAATCCCGGGCAGCGGACTAACGCGCGCGTACTCGTTCAGTTCG
CCGCCGGGCGCGTCTCAGGCGGCCTTCGTCGTGCGCAATGTGCCACAGGGCCGCATGAGCGGCTTTCTGGCCGGCGAGGC
CCAGACCGGACAGCGCATGGCATTCTCCGGCCCGTTCGGCAGCTTCTACCTGCGTTCCGTCACGCGGCCCGTTTTGTTCC
TGGCCGGCGGCACCGGCATCGCGCCGTTCCTGTCCATGCTCGACGTACTGGCCGCCAGCGGCAGCACGCACCCGGTGCGC
ATGGTCTACGGCGTCACGCACGACATCGACCTGGTGGCCACCGAGCGGCTCGAGGCCGCCGCGGCCAGCATCGACGGATT
CACCTACCGCACCTGCATTGCCGACGCCGCCAGCACGCACGAGCGCAAGGGCTATGTGACCACGCATGTCGACCCCACAT
GGATCAATCACGGGGACGTCGACATCTACCTGTGCGGCCCCGTGGCCATGGTGGAAGCCGTGCGCGACTGGCTGCGGGAT
GCCGGCATCACGCCGGCCAATTTCTACTACGAGAAGTTCTCGGCCAGCGCGGAGGCATGA

Upstream 100 bases:

>100_bases
AGTACGTCGTGCTCAAGAACGACTACATCCACCAGGTCATCGACATCTATCACATCTGATCCGCTCGCCGCATCAGGCAG
CATCACGGAGGGACAAGGCC

Downstream 100 bases:

>100_bases
TGCGCACAACGCAACGTTTCGCCGGCCGGACGATGATCGTCACGGGCGCGGCGCAGGGTATCGGTCGCGGCGTGGCAATG
TCCGCGGCTGCCGAGGGCGC

Product: benzoate 1,2-dioxygenase electron transfer component

Products: NA

Alternate protein names: Ferredoxin; Ferredoxin--NAD(+) reductase [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MEHTIALQFEDGVTRFITCGDNETLSDAAYRQQINIPLDCRDGACGTCRGLCESGHYDLPESSYIEDALTAEEAAKGYVL
ACKTRPRSDCVIRVPASSAACKTGVTRFEGAIAAVAPLSDSTIGFSIDLDDPAALSFLPGQYVNVEIPGSGLTRAYSFSS
PPGASQAAFVVRNVPQGRMSGFLAGEAQTGQRMAFSGPFGSFYLRSVTRPVLFLAGGTGIAPFLSMLDVLAASGSTHPVR
MVYGVTHDIDLVATERLEAAAASIDGFTYRTCIADAASTHERKGYVTTHVDPTWINHGDVDIYLCGPVAMVEAVRDWLRD
AGITPANFYYEKFSASAEA

Sequences:

>Translated_339_residues
MEHTIALQFEDGVTRFITCGDNETLSDAAYRQQINIPLDCRDGACGTCRGLCESGHYDLPESSYIEDALTAEEAAKGYVL
ACKTRPRSDCVIRVPASSAACKTGVTRFEGAIAAVAPLSDSTIGFSIDLDDPAALSFLPGQYVNVEIPGSGLTRAYSFSS
PPGASQAAFVVRNVPQGRMSGFLAGEAQTGQRMAFSGPFGSFYLRSVTRPVLFLAGGTGIAPFLSMLDVLAASGSTHPVR
MVYGVTHDIDLVATERLEAAAASIDGFTYRTCIADAASTHERKGYVTTHVDPTWINHGDVDIYLCGPVAMVEAVRDWLRD
AGITPANFYYEKFSASAEA
>Mature_339_residues
MEHTIALQFEDGVTRFITCGDNETLSDAAYRQQINIPLDCRDGACGTCRGLCESGHYDLPESSYIEDALTAEEAAKGYVL
ACKTRPRSDCVIRVPASSAACKTGVTRFEGAIAAVAPLSDSTIGFSIDLDDPAALSFLPGQYVNVEIPGSGLTRAYSFSS
PPGASQAAFVVRNVPQGRMSGFLAGEAQTGQRMAFSGPFGSFYLRSVTRPVLFLAGGTGIAPFLSMLDVLAASGSTHPVR
MVYGVTHDIDLVATERLEAAAASIDGFTYRTCIADAASTHERKGYVTTHVDPTWINHGDVDIYLCGPVAMVEAVRDWLRD
AGITPANFYYEKFSASAEA

Specific function: Electron transfer component of benzoate 1,2-dioxygenase system [H]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=274, Percent_Identity=27.007299270073, Blast_Score=80, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322146, Length=195, Percent_Identity=30.7692307692308, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: =1.18.1.3 [H]

Molecular weight: Translated: 36117; Mature: 36117

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHTIALQFEDGVTRFITCGDNETLSDAAYRQQINIPLDCRDGACGTCRGLCESGHYDLP
CCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHCCCCEECCCCCCHHHHHHHCCCCCCCC
ESSYIEDALTAEEAAKGYVLACKTRPRSDCVIRVPASSAACKTGVTRFEGAIAAVAPLSD
CCHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEECCCCCHHHHCCHHHCCCEEEEECCCC
STIGFSIDLDDPAALSFLPGQYVNVEIPGSGLTRAYSFSSPPGASQAAFVVRNVPQGRMS
CCEEEEEECCCCCEEEECCCCEEEEEECCCCCCEEEECCCCCCCCCEEEEEECCCCHHHC
GFLAGEAQTGQRMAFSGPFGSFYLRSVTRPVLFLAGGTGIAPFLSMLDVLAASGSTHPVR
EEEECCCCCCCEEEECCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEE
MVYGVTHDIDLVATERLEAAAASIDGFTYRTCIADAASTHERKGYVTTHVDPTWINHGDV
EEEECCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEECCCCCEECCCCE
DIYLCGPVAMVEAVRDWLRDAGITPANFYYEKFSASAEA
EEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MEHTIALQFEDGVTRFITCGDNETLSDAAYRQQINIPLDCRDGACGTCRGLCESGHYDLP
CCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHCCCCEECCCCCCHHHHHHHCCCCCCCC
ESSYIEDALTAEEAAKGYVLACKTRPRSDCVIRVPASSAACKTGVTRFEGAIAAVAPLSD
CCHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEECCCCCHHHHCCHHHCCCEEEEECCCC
STIGFSIDLDDPAALSFLPGQYVNVEIPGSGLTRAYSFSSPPGASQAAFVVRNVPQGRMS
CCEEEEEECCCCCEEEECCCCEEEEEECCCCCCEEEECCCCCCCCCEEEEEECCCCHHHC
GFLAGEAQTGQRMAFSGPFGSFYLRSVTRPVLFLAGGTGIAPFLSMLDVLAASGSTHPVR
EEEECCCCCCCEEEECCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEE
MVYGVTHDIDLVATERLEAAAASIDGFTYRTCIADAASTHERKGYVTTHVDPTWINHGDV
EEEECCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEECCCCCEECCCCE
DIYLCGPVAMVEAVRDWLRDAGITPANFYYEKFSASAEA
EEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1885518 [H]