BacMap

Definition	Cupriavidus metallidurans CH34 megaplasmid, complete sequence.
Accession	NC_007974
Length	2,580,084

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The map label for this gene is benD [H]

Identifier: 94313807

GI number: 94313807

Start: 1547054

End: 1547848

Strand: Direct

Name: benD [H]

Synonym: Rmet_4885

Alternate gene names: 94313807

Gene position: 1547054-1547848 (Clockwise)

Preceding gene: 94313806

Following gene: 94313812

Centisome position: 59.96

GC content: 66.54

Gene sequence:

>795_bases
ATGCGCACAACGCAACGTTTCGCCGGCCGGACGATGATCGTCACGGGCGCGGCGCAGGGTATCGGTCGCGGCGTGGCAAT
GTCCGCGGCTGCCGAGGGCGCGAATGTGGTGCTGGTGGACCGCGCCACGCTGGTCGAGGAAGTCGCGGCCGGGATTCTGG
CTGCGGGCGGCGCGGCACTGTCCGTCAATGCTGATCTGGAGACGTATGCGGGGGCGCAGGCGATGGTAGCCACGGCCACC
GCTGCCTTCGGCCATGTCGATATCCTGATCAACAACGTGGGCGGCACGATCTGGGCCAAGCCCTATGAGCACTACGCTGA
AGCCGAGATCGAGGCCGAGATCCGCCGGTCGCTGTTTCCCACGCTCTGGGGTTGCCGCGCGGTGTTGCCGGGCATGGTCG
AGCGTCGCGCCGGAGTCATCGTCAACGTGTCGTCGATTGCGACGCGCGGCATCCATCGCGTGCCCTACGCGGCGGCCAAA
GGCGGCGTGAACGCGCTGACCGCGAGCCTGGCGTTCGAGCACGCGGCGGATGGCATTCGCATTAACGCGGTGGCTACGGG
CGGCACCGAGGCGCCGCCGCGCAGGATCCCGCGCAACACGCAGCAACAATCGGAACAGTCGGAACAGGAATCGAAGTGGT
ATCAGGCAATCGTCGATCAGACCGTGTCGTCGAGCCTGATGCACCGGTACGGGACGATCGAGGAACAAGTGGGTGTGATC
CTGTTCCTGGCCTCGGACGAGGCGTCGTACATGACGGGGACTGTGGTGCCGGTGGGTGGCGGAGACCTTGGCTGA

Upstream 100 bases:

>100_bases
CCGTGGCCATGGTGGAAGCCGTGCGCGACTGGCTGCGGGATGCCGGCATCACGCCGGCCAATTTCTACTACGAGAAGTTC
TCGGCCAGCGCGGAGGCATG

Downstream 100 bases:

>100_bases
TCGCGCTGACTTGCCGCTTGATCGCGCCCCTCTCCGATTTGCTTGGGAGGGGGCGCGCATGACAGGCGATCACGCGCACG
ATGGCTATGCAGTGACGATC

Product: 1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase

Products: NA

Alternate protein names: 2-hydro-1,2-dihydroxybenzoate dehydrogenase; DHB dehydrogenase; Cis-1,2-dihydroxy-3,4-cyclohexadiene-1-carboxylate dehydrogenase; Cis-1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate dehydrogenase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MRTTQRFAGRTMIVTGAAQGIGRGVAMSAAAEGANVVLVDRATLVEEVAAGILAAGGAALSVNADLETYAGAQAMVATAT
AAFGHVDILINNVGGTIWAKPYEHYAEAEIEAEIRRSLFPTLWGCRAVLPGMVERRAGVIVNVSSIATRGIHRVPYAAAK
GGVNALTASLAFEHAADGIRINAVATGGTEAPPRRIPRNTQQQSEQSEQESKWYQAIVDQTVSSSLMHRYGTIEEQVGVI
LFLASDEASYMTGTVVPVGGGDLG

Sequences:

>Translated_264_residues
MRTTQRFAGRTMIVTGAAQGIGRGVAMSAAAEGANVVLVDRATLVEEVAAGILAAGGAALSVNADLETYAGAQAMVATAT
AAFGHVDILINNVGGTIWAKPYEHYAEAEIEAEIRRSLFPTLWGCRAVLPGMVERRAGVIVNVSSIATRGIHRVPYAAAK
GGVNALTASLAFEHAADGIRINAVATGGTEAPPRRIPRNTQQQSEQSEQESKWYQAIVDQTVSSSLMHRYGTIEEQVGVI
LFLASDEASYMTGTVVPVGGGDLG
>Mature_264_residues
MRTTQRFAGRTMIVTGAAQGIGRGVAMSAAAEGANVVLVDRATLVEEVAAGILAAGGAALSVNADLETYAGAQAMVATAT
AAFGHVDILINNVGGTIWAKPYEHYAEAEIEAEIRRSLFPTLWGCRAVLPGMVERRAGVIVNVSSIATRGIHRVPYAAAK
GGVNALTASLAFEHAADGIRINAVATGGTEAPPRRIPRNTQQQSEQSEQESKWYQAIVDQTVSSSLMHRYGTIEEQVGVI
LFLASDEASYMTGTVVPVGGGDLG

Specific function: Degradation of 2-hydro-1,2-dihydroxy benzoate (DHB) to catechol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI19923817, Length=260, Percent_Identity=29.6153846153846, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI32483357, Length=274, Percent_Identity=29.1970802919708, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI7705925, Length=264, Percent_Identity=29.5454545454545, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI15277342, Length=263, Percent_Identity=29.277566539924, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI66933014, Length=268, Percent_Identity=28.7313432835821, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI126723750, Length=263, Percent_Identity=26.615969581749, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI4504505, Length=199, Percent_Identity=31.1557788944724, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI40254992, Length=256, Percent_Identity=27.34375, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI10190704, Length=259, Percent_Identity=28.957528957529, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI87082100, Length=260, Percent_Identity=31.9230769230769, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1789208, Length=259, Percent_Identity=30.8880308880309, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1787335, Length=257, Percent_Identity=32.295719844358, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI2367175, Length=261, Percent_Identity=32.5670498084291, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1787905, Length=259, Percent_Identity=28.957528957529, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1790717, Length=270, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789378, Length=261, Percent_Identity=27.9693486590038, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI87082160, Length=257, Percent_Identity=25.2918287937743, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17559104, Length=280, Percent_Identity=33.2142857142857, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71994600, Length=266, Percent_Identity=30.8270676691729, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71994604, Length=265, Percent_Identity=30.5660377358491, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17562904, Length=268, Percent_Identity=30.9701492537313, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17562990, Length=272, Percent_Identity=29.4117647058824, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17562910, Length=275, Percent_Identity=29.8181818181818, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17565030, Length=274, Percent_Identity=31.7518248175182, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17538486, Length=276, Percent_Identity=29.7101449275362, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17531453, Length=276, Percent_Identity=31.5217391304348, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17560150, Length=269, Percent_Identity=30.1115241635688, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17538480, Length=277, Percent_Identity=30.6859205776173, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI193204405, Length=194, Percent_Identity=31.4432989690722, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17563726, Length=202, Percent_Identity=31.1881188118812, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17551412, Length=201, Percent_Identity=30.8457711442786, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17544670, Length=193, Percent_Identity=31.0880829015544, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI72000259, Length=265, Percent_Identity=27.1698113207547, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17562906, Length=196, Percent_Identity=31.6326530612245, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562908, Length=199, Percent_Identity=31.1557788944724, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17560220, Length=202, Percent_Identity=30.1980198019802, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17564282, Length=168, Percent_Identity=30.952380952381, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560332, Length=284, Percent_Identity=28.169014084507, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17568967, Length=209, Percent_Identity=30.622009569378, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17561402, Length=278, Percent_Identity=24.8201438848921, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71982365, Length=196, Percent_Identity=29.0816326530612, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI25152911, Length=184, Percent_Identity=35.3260869565217, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17560676, Length=261, Percent_Identity=24.1379310344828, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=191, Percent_Identity=30.8900523560209, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571526, Length=265, Percent_Identity=32.8301886792453, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21357041, Length=265, Percent_Identity=33.2075471698113, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24644339, Length=266, Percent_Identity=31.203007518797, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI23397609, Length=263, Percent_Identity=31.5589353612167, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24639444, Length=260, Percent_Identity=28.8461538461538, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21355319, Length=271, Percent_Identity=27.6752767527675, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21357673, Length=198, Percent_Identity=29.2929292929293, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24644337, Length=268, Percent_Identity=29.4776119402985, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.25 [H]

Molecular weight: Translated: 27552; Mature: 27552

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTTQRFAGRTMIVTGAAQGIGRGVAMSAAAEGANVVLVDRATLVEEVAAGILAAGGAAL
CCCCHHHCCCEEEEEECCHHCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCEEE
SVNADLETYAGAQAMVATATAAFGHVDILINNVGGTIWAKPYEHYAEAEIEAEIRRSLFP
EECCCHHHHCCCHHHHHHHHHHHCEEEEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHH
TLWGCRAVLPGMVERRAGVIVNVSSIATRGIHRVPYAAAKGGVNALTASLAFEHAADGIR
HHHHHHHHHHHHHHHHCCEEEEHHHHHHCCHHHCCCHHHCCCHHHHHHHHHHHHCCCCEE
INAVATGGTEAPPRRIPRNTQQQSEQSEQESKWYQAIVDQTVSSSLMHRYGTIEEQVGVI
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEE
LFLASDEASYMTGTVVPVGGGDLG
EEEECCCCCEEEEEEEECCCCCCC
>Mature Secondary Structure
MRTTQRFAGRTMIVTGAAQGIGRGVAMSAAAEGANVVLVDRATLVEEVAAGILAAGGAAL
CCCCHHHCCCEEEEEECCHHCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCEEE
SVNADLETYAGAQAMVATATAAFGHVDILINNVGGTIWAKPYEHYAEAEIEAEIRRSLFP
EECCCHHHHCCCHHHHHHHHHHHCEEEEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHH
TLWGCRAVLPGMVERRAGVIVNVSSIATRGIHRVPYAAAKGGVNALTASLAFEHAADGIR
HHHHHHHHHHHHHHHHCCEEEEHHHHHHCCHHHCCCHHHCCCHHHHHHHHHHHHCCCCEE
INAVATGGTEAPPRRIPRNTQQQSEQSEQESKWYQAIVDQTVSSSLMHRYGTIEEQVGVI
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEE
LFLASDEASYMTGTVVPVGGGDLG
EEEECCCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1740120 [H]