Click here to switch to the map view.

The map label for this gene is catB [H]

Identifier: 94313800

GI number: 94313800

Start: 1539789

End: 1540913

Strand: Reverse

Name: catB [H]

Synonym: Rmet_4878

Alternate gene names: 94313800

Gene position: 1540913-1539789 (Counterclockwise)

Preceding gene: 94313802

Following gene: 94313799

Centisome position: 59.72

GC content: 65.69

Gene sequence:

>1125_bases
ATGACAGCGACGATTTCTTCCATCGAGGCCATTCTGGTCGACCTGCCCACGATCCGGGCGCATCAGTTGGCCATGGCCAC
GATGCAGCAGCAGACGCTGGTGATCGTGCGGCTGCGCTGCAGCGACGGGATCGAGGGCATCGGTGAGGCCACTACAATTG
GCGGCCTGTCGTACGGCGACGAAAGTCCCGAGGGCATCAAGCTGACGATCGATACCTACCTGGCGCCGGCACTGGTCGGC
CAGGACGCCACCAATGTCCACGCCGCGATGGCGCGCCTGAACAAGGTCGCGCGCGGTAACCGGTTCGCCAAGTCGGCGCT
GGAATCGGCCATGCTCGACGCGCAGGGCAAGCGTCTGGACGTGCCGCTGGCCACGCTGCTGGGTGGCGCGGTGCGCGACA
CGCTGCCGGTGCTCTGGACACTGGCCAGCGGTGATACCGCACGCGATATCGAGGAAGCCGAGCGCTTTCTGGAGGAACGC
CGCCACAACACGTTCAAGCTCAAGATCGGTCGCCGCAGCGTGCGTGAAGATGTGGCGCATGTCTCGGCGATCAAGCGCGC
CCTGGGCGATCGCGCCCGCGTGACGGTAGACGTGAACCAGGCCTGGAACGAAGCCGATGCCGCCACCGGCATCGCCATGC
TCGAAGCGGCCGGCATCGACCTGATCGAACAACCGACGCCGCGCGAGCAACGCACGGCGCTGGCGCGTCTGGCCGCCCGT
TTCGTGGTGCCGATCATGGCCGATGAAGCCGTGACGGGCCCCGAGGATGCAATGGAACTGGCACGCCTGGGCGCCGCCGA
TGTGTTCGCGCTGAAGATCGCCAAATCCGGTGGCATCTTCGGCATGTTGCGGACTGCGGCCGTGGGCGATGCAGCAGGTA
TCGCGCTCTACGGTGGCACGATGCTCGAAGGCAGCGTCGGCACGATCGCCGCGGCGCATGGCTTCTGCACGCTGCCGCAA
CTGGCGTGGGGCACGGAATTGTTTGGCCCGCTGCTGGTGAAGGACGATATCGTTGTCGAGCGCCCCGTATTCAAGGACTT
CTCGCTGCATCTGCCGCAGGGTCCGGGGCTGGGTCTGGCAATCGACGAAGACAAGCTTGCGCACTATCGTCGCAAGTCCG
CTTGA

Upstream 100 bases:

>100_bases
AGGTATGAGATGAGACATCTTCGGTCTTGGACGCTTAGCAAGTCAAGGTACATACTCCGTGCACGAGACTAATTTGCATG
GAAAAGGTATGACAGCGGGT

Downstream 100 bases:

>100_bases
TTGCCTGATCGACCTACCACCTAATCGCATCCTTCAAGCACAAGGAGACCCCAATGCTGTTTCTCGTCAGGATGGACGTT
CAGATTCCGCATGATTTGCC

Product: muconate cycloisomerase

Products: NA

Alternate protein names: Cis,cis-muconate lactonizing enzyme I; MLE; Muconate cycloisomerase I [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MTATISSIEAILVDLPTIRAHQLAMATMQQQTLVIVRLRCSDGIEGIGEATTIGGLSYGDESPEGIKLTIDTYLAPALVG
QDATNVHAAMARLNKVARGNRFAKSALESAMLDAQGKRLDVPLATLLGGAVRDTLPVLWTLASGDTARDIEEAERFLEER
RHNTFKLKIGRRSVREDVAHVSAIKRALGDRARVTVDVNQAWNEADAATGIAMLEAAGIDLIEQPTPREQRTALARLAAR
FVVPIMADEAVTGPEDAMELARLGAADVFALKIAKSGGIFGMLRTAAVGDAAGIALYGGTMLEGSVGTIAAAHGFCTLPQ
LAWGTELFGPLLVKDDIVVERPVFKDFSLHLPQGPGLGLAIDEDKLAHYRRKSA

Sequences:

>Translated_374_residues
MTATISSIEAILVDLPTIRAHQLAMATMQQQTLVIVRLRCSDGIEGIGEATTIGGLSYGDESPEGIKLTIDTYLAPALVG
QDATNVHAAMARLNKVARGNRFAKSALESAMLDAQGKRLDVPLATLLGGAVRDTLPVLWTLASGDTARDIEEAERFLEER
RHNTFKLKIGRRSVREDVAHVSAIKRALGDRARVTVDVNQAWNEADAATGIAMLEAAGIDLIEQPTPREQRTALARLAAR
FVVPIMADEAVTGPEDAMELARLGAADVFALKIAKSGGIFGMLRTAAVGDAAGIALYGGTMLEGSVGTIAAAHGFCTLPQ
LAWGTELFGPLLVKDDIVVERPVFKDFSLHLPQGPGLGLAIDEDKLAHYRRKSA
>Mature_373_residues
TATISSIEAILVDLPTIRAHQLAMATMQQQTLVIVRLRCSDGIEGIGEATTIGGLSYGDESPEGIKLTIDTYLAPALVGQ
DATNVHAAMARLNKVARGNRFAKSALESAMLDAQGKRLDVPLATLLGGAVRDTLPVLWTLASGDTARDIEEAERFLEERR
HNTFKLKIGRRSVREDVAHVSAIKRALGDRARVTVDVNQAWNEADAATGIAMLEAAGIDLIEQPTPREQRTALARLAARF
VVPIMADEAVTGPEDAMELARLGAADVFALKIAKSGGIFGMLRTAAVGDAAGIALYGGTMLEGSVGTIAAAHGFCTLPQL
AWGTELFGPLLVKDDIVVERPVFKDFSLHLPQGPGLGLAIDEDKLAHYRRKSA

Specific function: Catalyzes a syn cycloisomerization [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=350, Percent_Identity=25.1428571428571, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87081876, Length=295, Percent_Identity=25.7627118644068, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013370
- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.5.1.1 [H]

Molecular weight: Translated: 39820; Mature: 39689

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATISSIEAILVDLPTIRAHQLAMATMQQQTLVIVRLRCSDGIEGIGEATTIGGLSYGD
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCEECCCCCCC
ESPEGIKLTIDTYLAPALVGQDATNVHAAMARLNKVARGNRFAKSALESAMLDAQGKRLD
CCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEC
VPLATLLGGAVRDTLPVLWTLASGDTARDIEEAERFLEERRHNTFKLKIGRRSVREDVAH
CCHHHHHCHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHH
VSAIKRALGDRARVTVDVNQAWNEADAATGIAMLEAAGIDLIEQPTPREQRTALARLAAR
HHHHHHHHCCCEEEEEEHHHCCCHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHH
FVVPIMADEAVTGPEDAMELARLGAADVFALKIAKSGGIFGMLRTAAVGDAAGIALYGGT
HHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCEEEECCE
MLEGSVGTIAAAHGFCTLPQLAWGTELFGPLLVKDDIVVERPVFKDFSLHLPQGPGLGLA
EECCCCCHHHHHCCCCCCCHHHHCHHHHCCEEECCCEEECCCCCCCCEEECCCCCCCEEE
IDEDKLAHYRRKSA
ECHHHHHHHHHCCC
>Mature Secondary Structure 
TATISSIEAILVDLPTIRAHQLAMATMQQQTLVIVRLRCSDGIEGIGEATTIGGLSYGD
CCCHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCEECCCCCCC
ESPEGIKLTIDTYLAPALVGQDATNVHAAMARLNKVARGNRFAKSALESAMLDAQGKRLD
CCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEC
VPLATLLGGAVRDTLPVLWTLASGDTARDIEEAERFLEERRHNTFKLKIGRRSVREDVAH
CCHHHHHCHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHH
VSAIKRALGDRARVTVDVNQAWNEADAATGIAMLEAAGIDLIEQPTPREQRTALARLAAR
HHHHHHHHCCCEEEEEEHHHCCCHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHH
FVVPIMADEAVTGPEDAMELARLGAADVFALKIAKSGGIFGMLRTAAVGDAAGIALYGGT
HHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCEEEECCE
MLEGSVGTIAAAHGFCTLPQLAWGTELFGPLLVKDDIVVERPVFKDFSLHLPQGPGLGLA
EECCCCCHHHHHCCCCCCCHHHHCHHHHCCEEECCCEEECCCCCCCCEEECCCCCCCEEE
IDEDKLAHYRRKSA
ECHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3609743; 2449420; 2215699; 3612800; 7500361 [H]