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The map label for this gene is cpg2 [H]

Identifier: 94313792

GI number: 94313792

Start: 1531863

End: 1533116

Strand: Reverse

Name: cpg2 [H]

Synonym: Rmet_4870

Alternate gene names: 94313792

Gene position: 1533116-1531863 (Counterclockwise)

Preceding gene: 94313794

Following gene: 94313790

Centisome position: 59.42

GC content: 65.63

Gene sequence:

>1254_bases
ATGCTGCTTTCCCTTCGCCACCTTGCCATCGCCGCTGCCGCTGCCGTGGTCTGCAATGCCGCCGTGGCTGCCGGCGCCGA
TACCGGGTTGCTCGATGCCGCGCGGTCCGCACAGCCGGCCGTGGTGCAGTCGCTCAAGGACATGGTGTCGATAGAGTCGG
GCAGCGCCAATATCGAAGGGCTCGACCGCATGGCGGACTACACGTCGAAGCGGCTCCAGGCGTTGGGTGCCAAGGTGGAG
CGTGTGCCGGCAAGCTCGGGCAAGAGCCCGATCGTCAAGGCGACGCTGACCGGTACGGGCAAGCACAAGATCATGTTGAT
TGCCCATATGGATACGGTCTATCCGGCCGGTATCCTCAAGACGCAGCCGATCCGCGAGGACGGCAACCGTCTCTATGGTC
CCGGCATCGCCGACGACAAGGGCGGCATCGCCGTGATCCTGCATTCGCTGGAAATCCTCAAGGCGCAGGGATGGAAGGAC
TACGCGCAACTGACCGTGCTGTTCAACGCCGATGAGGAAGTCGGTTCGGGCGGATCGGGCGAAACGATTGCGGCGCTGGC
CGATCAGCAGGACGTGGTGTTGTCCTGCGAGCCGAACGTGGCCAAGTCTGTCGCCAAGTCGGAGTCGCTGCTGCTGGGCG
CTGCTGGCACGGCGACTGCGACGATGCAGGTCAAGGGCCGCTCGTCGCACGCCGGCGCGGCGCCGGAGCTGGGCCGCAAT
GCGCTGATCGAACTGGCCTATCAGTTGCAGCAGACGCGTGACGTGGCCAAGTCGGTGCCTGGCTCTCAACTGAACTGGAC
CATGGCCAAGGGCGGCGACGTAGGCAACCAGATCCCCGAGTTGGCCAGTGCGTATGGCGATGTGCGTGTCACGGCCAACG
GCGCCGCGCAGAAGCTGCAGACCGCGCTGCAGGAGAAGGTCAACGCGGGCCACCTGATTCCCGACACGCAGACCACGGTG
ACGATGGAAGAGGGCCGCCCCGCCTACGTGGCTGACGCCCGCGCGAAGGATCTGGCCAAGCGCGCGCAGCAGGTCTACGC
GGAACTCGACGGCCGCTCGCTGCTGCTGATCCCTGGCACGGGTGGCGGCACGGACGCCGGCTATGCGGGGCGGTCGGGCA
AGGCGGTTGTGCTGGAGAGCTTCGGCCTGTCTGGCTTCGGCTACCACGCTCGCGACGAATACGTGGAGCTCGATTCGATC
GTGCCCCGTCTGTATCTGATGACGCGGATGCTGCAGGAGATCGGAAAGAACTGA

Upstream 100 bases:

>100_bases
CGGGGTGTGGCGGGATCGGCTTTGGGAATGGCGATATTGAGCCTCCCCGCGAGATATCGCAAGGGCTACATTTGAGGCTT
CCATCAGAACAGGAGACGTC

Downstream 100 bases:

>100_bases
ACGTATCGAATGCTGCGGCAAGTTTCCCTCTCCCGCGCAAACGGGAGAGGGAACAAGCAGGCGGTGACAACTACGCCGCC
ACCGCCTGCGGGGCCGCGCC

Product: glutamate carboxypeptidase

Products: NA

Alternate protein names: CPDG2; Folate hydrolase G2; Glutamate carboxypeptidase; Pteroylmonoglutamic acid hydrolase G2; Glucarpidase [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MLLSLRHLAIAAAAAVVCNAAVAAGADTGLLDAARSAQPAVVQSLKDMVSIESGSANIEGLDRMADYTSKRLQALGAKVE
RVPASSGKSPIVKATLTGTGKHKIMLIAHMDTVYPAGILKTQPIREDGNRLYGPGIADDKGGIAVILHSLEILKAQGWKD
YAQLTVLFNADEEVGSGGSGETIAALADQQDVVLSCEPNVAKSVAKSESLLLGAAGTATATMQVKGRSSHAGAAPELGRN
ALIELAYQLQQTRDVAKSVPGSQLNWTMAKGGDVGNQIPELASAYGDVRVTANGAAQKLQTALQEKVNAGHLIPDTQTTV
TMEEGRPAYVADARAKDLAKRAQQVYAELDGRSLLLIPGTGGGTDAGYAGRSGKAVVLESFGLSGFGYHARDEYVELDSI
VPRLYLMTRMLQEIGKN

Sequences:

>Translated_417_residues
MLLSLRHLAIAAAAAVVCNAAVAAGADTGLLDAARSAQPAVVQSLKDMVSIESGSANIEGLDRMADYTSKRLQALGAKVE
RVPASSGKSPIVKATLTGTGKHKIMLIAHMDTVYPAGILKTQPIREDGNRLYGPGIADDKGGIAVILHSLEILKAQGWKD
YAQLTVLFNADEEVGSGGSGETIAALADQQDVVLSCEPNVAKSVAKSESLLLGAAGTATATMQVKGRSSHAGAAPELGRN
ALIELAYQLQQTRDVAKSVPGSQLNWTMAKGGDVGNQIPELASAYGDVRVTANGAAQKLQTALQEKVNAGHLIPDTQTTV
TMEEGRPAYVADARAKDLAKRAQQVYAELDGRSLLLIPGTGGGTDAGYAGRSGKAVVLESFGLSGFGYHARDEYVELDSI
VPRLYLMTRMLQEIGKN
>Mature_417_residues
MLLSLRHLAIAAAAAVVCNAAVAAGADTGLLDAARSAQPAVVQSLKDMVSIESGSANIEGLDRMADYTSKRLQALGAKVE
RVPASSGKSPIVKATLTGTGKHKIMLIAHMDTVYPAGILKTQPIREDGNRLYGPGIADDKGGIAVILHSLEILKAQGWKD
YAQLTVLFNADEEVGSGGSGETIAALADQQDVVLSCEPNVAKSVAKSESLLLGAAGTATATMQVKGRSSHAGAAPELGRN
ALIELAYQLQQTRDVAKSVPGSQLNWTMAKGGDVGNQIPELASAYGDVRVTANGAAQKLQTALQEKVNAGHLIPDTQTTV
TMEEGRPAYVADARAKDLAKRAQQVYAELDGRSLLLIPGTGGGTDAGYAGRSGKAVVLESFGLSGFGYHARDEYVELDSI
VPRLYLMTRMLQEIGKN

Specific function: Catalyzes the hydrolysis of reduced and non-reduced folates to pteroates and L-glutamate. This enzyme has a broad specificity [H]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family [H]

Homologues:

Organism=Escherichia coli, GI1790395, Length=278, Percent_Identity=26.978417266187, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR017150
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.4.17.11 [H]

Molecular weight: Translated: 43516; Mature: 43516

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00107 PROTEIN_KINASE_ATP ; PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSLRHLAIAAAAAVVCNAAVAAGADTGLLDAARSAQPAVVQSLKDMVSIESGSANIEG
CEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHEECCCCCCHHH
LDRMADYTSKRLQALGAKVERVPASSGKSPIVKATLTGTGKHKIMLIAHMDTVYPAGILK
HHHHHHHHHHHHHHHCCHHHCCCCCCCCCCEEEEEEECCCCEEEEEEEEECCCCCCCCCC
TQPIREDGNRLYGPGIADDKGGIAVILHSLEILKAQGWKDYAQLTVLFNADEEVGSGGSG
CCCCHHCCCEEECCCCCCCCCCCEEEHHHHHHHHHCCCCCEEEEEEEEECCHHHCCCCCC
ETIAALADQQDVVLSCEPNVAKSVAKSESLLLGAAGTATATMQVKGRSSHAGAAPELGRN
CEEEEECCCCCEEEECCCHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCCCHHHHH
ALIELAYQLQQTRDVAKSVPGSQLNWTMAKGGDVGNQIPELASAYGDVRVTANGAAQKLQ
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEECCHHHHHH
TALQEKVNAGHLIPDTQTTVTMEEGRPAYVADARAKDLAKRAQQVYAELDGRSLLLIPGT
HHHHHHCCCCCCCCCCCEEEEECCCCCEEEECHHHHHHHHHHHHHHHHCCCCEEEEEECC
GGGTDAGYAGRSGKAVVLESFGLSGFGYHARDEYVELDSIVPRLYLMTRMLQEIGKN
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLLSLRHLAIAAAAAVVCNAAVAAGADTGLLDAARSAQPAVVQSLKDMVSIESGSANIEG
CEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHEECCCCCCHHH
LDRMADYTSKRLQALGAKVERVPASSGKSPIVKATLTGTGKHKIMLIAHMDTVYPAGILK
HHHHHHHHHHHHHHHCCHHHCCCCCCCCCCEEEEEEECCCCEEEEEEEEECCCCCCCCCC
TQPIREDGNRLYGPGIADDKGGIAVILHSLEILKAQGWKDYAQLTVLFNADEEVGSGGSG
CCCCHHCCCEEECCCCCCCCCCCEEEHHHHHHHHHCCCCCEEEEEEEEECCHHHCCCCCC
ETIAALADQQDVVLSCEPNVAKSVAKSESLLLGAAGTATATMQVKGRSSHAGAAPELGRN
CEEEEECCCCCEEEECCCHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCCCHHHHH
ALIELAYQLQQTRDVAKSVPGSQLNWTMAKGGDVGNQIPELASAYGDVRVTANGAAQKLQ
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEECCHHHHHH
TALQEKVNAGHLIPDTQTTVTMEEGRPAYVADARAKDLAKRAQQVYAELDGRSLLLIPGT
HHHHHHCCCCCCCCCCCEEEEECCCCCEEEECHHHHHHHHHHHHHHHHCCCCEEEEEECC
GGGTDAGYAGRSGKAVVLESFGLSGFGYHARDEYVELDSIVPRLYLMTRMLQEIGKN
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6396165; 3838935; 9083113 [H]