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The map label for this gene is llpE [H]

Identifier: 94313790

GI number: 94313790

Start: 1529618

End: 1530493

Strand: Reverse

Name: llpE [H]

Synonym: Rmet_4868

Alternate gene names: 94313790

Gene position: 1530493-1529618 (Counterclockwise)

Preceding gene: 94313792

Following gene: 94313789

Centisome position: 59.32

GC content: 65.87

Gene sequence:

>876_bases
ATGACCAAGACCACCACAGCCCGTAGTTCCGCCGTGCAACCGGCCGCAGAACAGGGCGAACCGTACGTGGTGCAGCATAT
GGTCGTCAGTGGCGATCGGGAGATCAAGGTCTGCGTGTGTTACCCCGCAGGTTATTGGCCGTTGAGCGCAGGCTCGGCAA
CCTTGCCATGGCTGGTGTATCTCCATGCGGGCGGTTTTGTCGAAGGTGACGTCGAGGCAGCCAAGGAACTGGCGCGGGAT
CTGGCGGAGTCGGTCCCGGCCGTGGTGGTGACGCCGGGATACTCGCTGGCGCCCGAAAATCCGTTCCCGGCCGCGCCCGA
AGACGCCGTGGCCACCGTGACGTGGGTACTGAAGCAGGCAAAGAAGCTCAAGGTCGACAAGCTGCGCTTCGCGCTGGTCG
GCGAAGAGGCGGGCGGCAACCTGGCAATTGCCACGGCGCAGATGCTGCGGGATCGCGGCCTGCCGCAGCCGGCGGGCCAG
TGGCTGATCCGCCCCGTCACGGACCCGTGCCTGTCGGCGATGTCTTCGACGCGTCAGGTGCCGATGGAAATGCTGCGCCG
GCTGGCCTGCAACTATCGCGAATATCTGCCGACGCCGGCTGACAGCGTGCATCCATATGCGGCGCCGGCACTGGCATCGC
GGATGGCCGGCCTGCCTGCCACGCTGGTGCAGGTAGCGGAACAAGACGCCTTGCGTGCCGAAGGTGAAGCCTTCGCCGCG
AAGCTGGCCAAGGCCGGGATATCGGCCAGGGCCCTGATCATGCCCGGCGCCTGTGGCGACGGGGTGGAACAGTCCCATGA
GAAGTGTCAGGCATGGGTCGAAGAAGGTGCGCGGTTCCTGCGCGATTGTTTCGCGAAGGCCGACGACGCCTCGTAA

Upstream 100 bases:

>100_bases
GTGCGTTGGCAATAGCTCGCTTTATCCCATCATCGTCAATCACCATAATTCGGTGCATCGCAACACAGTATCGACGACCC
ATGCCGGTCGCCTGAACGCC

Downstream 100 bases:

>100_bases
CCGGTCCGAACACCGGCCGATACCGGGAGCATGACGGTATCGGATTCAACGGCCAGCGCCCTTTGAGGCTGGCAGCAGCA
ATCCCGTCCTGACCGGACGG

Product: esterase/lipase/thioesterase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTKTTTARSSAVQPAAEQGEPYVVQHMVVSGDREIKVCVCYPAGYWPLSAGSATLPWLVYLHAGGFVEGDVEAAKELARD
LAESVPAVVVTPGYSLAPENPFPAAPEDAVATVTWVLKQAKKLKVDKLRFALVGEEAGGNLAIATAQMLRDRGLPQPAGQ
WLIRPVTDPCLSAMSSTRQVPMEMLRRLACNYREYLPTPADSVHPYAAPALASRMAGLPATLVQVAEQDALRAEGEAFAA
KLAKAGISARALIMPGACGDGVEQSHEKCQAWVEEGARFLRDCFAKADDAS

Sequences:

>Translated_291_residues
MTKTTTARSSAVQPAAEQGEPYVVQHMVVSGDREIKVCVCYPAGYWPLSAGSATLPWLVYLHAGGFVEGDVEAAKELARD
LAESVPAVVVTPGYSLAPENPFPAAPEDAVATVTWVLKQAKKLKVDKLRFALVGEEAGGNLAIATAQMLRDRGLPQPAGQ
WLIRPVTDPCLSAMSSTRQVPMEMLRRLACNYREYLPTPADSVHPYAAPALASRMAGLPATLVQVAEQDALRAEGEAFAA
KLAKAGISARALIMPGACGDGVEQSHEKCQAWVEEGARFLRDCFAKADDAS
>Mature_290_residues
TKTTTARSSAVQPAAEQGEPYVVQHMVVSGDREIKVCVCYPAGYWPLSAGSATLPWLVYLHAGGFVEGDVEAAKELARDL
AESVPAVVVTPGYSLAPENPFPAAPEDAVATVTWVLKQAKKLKVDKLRFALVGEEAGGNLAIATAQMLRDRGLPQPAGQW
LIRPVTDPCLSAMSSTRQVPMEMLRRLACNYREYLPTPADSVHPYAAPALASRMAGLPATLVQVAEQDALRAEGEAFAAK
LAKAGISARALIMPGACGDGVEQSHEKCQAWVEEGARFLRDCFAKADDAS

Specific function: This protein removes the N-acetyl group from bialaphos as one of the final steps of the bialaphos biosynthetic pathway [H]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786682, Length=97, Percent_Identity=36.0824742268041, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI72001146, Length=299, Percent_Identity=23.4113712374582, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71996133, Length=124, Percent_Identity=31.4516129032258, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 30991; Mature: 30860

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTTTARSSAVQPAAEQGEPYVVQHMVVSGDREIKVCVCYPAGYWPLSAGSATLPWLVY
CCCCCCCHHHHCCCHHHCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCEEEE
LHAGGFVEGDVEAAKELARDLAESVPAVVVTPGYSLAPENPFPAAPEDAVATVTWVLKQA
EECCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KKLKVDKLRFALVGEEAGGNLAIATAQMLRDRGLPQPAGQWLIRPVTDPCLSAMSSTRQV
HHHHHHHHHEEEEECCCCCCEEHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHH
PMEMLRRLACNYREYLPTPADSVHPYAAPALASRMAGLPATLVQVAEQDALRAEGEAFAA
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
KLAKAGISARALIMPGACGDGVEQSHEKCQAWVEEGARFLRDCFAKADDAS
HHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TKTTTARSSAVQPAAEQGEPYVVQHMVVSGDREIKVCVCYPAGYWPLSAGSATLPWLVY
CCCCCCHHHHCCCHHHCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCEEEE
LHAGGFVEGDVEAAKELARDLAESVPAVVVTPGYSLAPENPFPAAPEDAVATVTWVLKQA
EECCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KKLKVDKLRFALVGEEAGGNLAIATAQMLRDRGLPQPAGQWLIRPVTDPCLSAMSSTRQV
HHHHHHHHHEEEEECCCCCCEEHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHH
PMEMLRRLACNYREYLPTPADSVHPYAAPALASRMAGLPATLVQVAEQDALRAEGEAFAA
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
KLAKAGISARALIMPGACGDGVEQSHEKCQAWVEEGARFLRDCFAKADDAS
HHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2066341 [H]