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The map label for this gene is cysU [H]

Identifier: 94313763

GI number: 94313763

Start: 1498432

End: 1499250

Strand: Direct

Name: cysU [H]

Synonym: Rmet_4841

Alternate gene names: 94313763

Gene position: 1498432-1499250 (Clockwise)

Preceding gene: 94313762

Following gene: 94313764

Centisome position: 58.08

GC content: 66.42

Gene sequence:

>819_bases
ATGACCAGACGATCCCCCGCCCTCTTCCTGTTCGCGCTGCCCGCGCTCGTCGTGTTCCTGGCGTTCTTCTGCCTGCCGAT
GGCACGGCTGATCGAAGTCAGCCTGACCGGCAAGGACGGCGCGGGGGTCTACTGGACCGTGCTGACCAGCGGCCGCTACC
TGCACAGCCTGATGGTGACCGTGCTGCTCTCGGCGCTGGTCACGCTGGCGACGCTGGTGATCGCCGGCATATCGGGCACG
TTCCTGCAACGCCATCCGGTGCCGGGCAAACCAGTGCTGGTGGCAATGCTGACGTTTCCGCTGGCCTTCCCAGGTGTGGT
GATCGGCTTCATGGTCATCATGCTTGGGGGCCGCAACGGCCTGCTGGCTTCGATTGGCGATGCGCTGACCGGCGAACGCT
GGACCTTCGCCTATGGCCTCGCCGGCCTGTTCGTTGGCTACCTGTACTTCTCGATTCCGCGCGTGATCCTGACGGTGATG
GCCGCCGTCGACAAGCTCGACACCTCGCTGGAGGAAGCGGCGCGCTCGCTCGGCGCCAGCCGCTGGCACGTGGTGCGCGA
CGTGATGCTGCCTGCGCTGATGCCGGCCATGCTGTCGAGCGGCGCAATCTGCTTTGCCACCAGCATGGGCGCGTTCGGCA
CGGCCTTCACGCTGGCCACCCAGCTCGACGTGCTGCCGCTGACGATCTACAACGAGTTCACCAACTACGCGAACTTCGGC
ATGGCCGCCGCCCTGTCGATCCTGCTTGGTGCTGTCACCTGGATGCTGCTGGCGCTGGCCCGCTGGTACTCCGGCGATGC
GGTCGCCGCTACCGCCTAG

Upstream 100 bases:

>100_bases
CCAAGCCGGTCGACTTCGGCAAGATGGCTCAGGTGCAGAAAGCCTTCAGCGAGCAGTACCTGAAGGAAGTCCGCTGATCC
AGGCGCCGCCAGACTCCATC

Downstream 100 bases:

>100_bases
AAGGCCCTATCAAAATGATTCTGGCGTCCTTGCGCGGCCTTGCCGTAACACTTGCAATGTCTGCGGCCGCGCGTCTAGCC
AGCACCGCTTCGCTGCATCT

Product: putative ABC transporter, membrane component

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MTRRSPALFLFALPALVVFLAFFCLPMARLIEVSLTGKDGAGVYWTVLTSGRYLHSLMVTVLLSALVTLATLVIAGISGT
FLQRHPVPGKPVLVAMLTFPLAFPGVVIGFMVIMLGGRNGLLASIGDALTGERWTFAYGLAGLFVGYLYFSIPRVILTVM
AAVDKLDTSLEEAARSLGASRWHVVRDVMLPALMPAMLSSGAICFATSMGAFGTAFTLATQLDVLPLTIYNEFTNYANFG
MAAALSILLGAVTWMLLALARWYSGDAVAATA

Sequences:

>Translated_272_residues
MTRRSPALFLFALPALVVFLAFFCLPMARLIEVSLTGKDGAGVYWTVLTSGRYLHSLMVTVLLSALVTLATLVIAGISGT
FLQRHPVPGKPVLVAMLTFPLAFPGVVIGFMVIMLGGRNGLLASIGDALTGERWTFAYGLAGLFVGYLYFSIPRVILTVM
AAVDKLDTSLEEAARSLGASRWHVVRDVMLPALMPAMLSSGAICFATSMGAFGTAFTLATQLDVLPLTIYNEFTNYANFG
MAAALSILLGAVTWMLLALARWYSGDAVAATA
>Mature_271_residues
TRRSPALFLFALPALVVFLAFFCLPMARLIEVSLTGKDGAGVYWTVLTSGRYLHSLMVTVLLSALVTLATLVIAGISGTF
LQRHPVPGKPVLVAMLTFPLAFPGVVIGFMVIMLGGRNGLLASIGDALTGERWTFAYGLAGLFVGYLYFSIPRVILTVMA
AVDKLDTSLEEAARSLGASRWHVVRDVMLPALMPAMLSSGAICFATSMGAFGTAFTLATQLDVLPLTIYNEFTNYANFGM
AAALSILLGAVTWMLLALARWYSGDAVAATA

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=207, Percent_Identity=29.951690821256, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786980, Length=218, Percent_Identity=28.4403669724771, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786254, Length=208, Percent_Identity=26.4423076923077, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI87082099, Length=215, Percent_Identity=26.046511627907, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787368, Length=178, Percent_Identity=26.9662921348315, Blast_Score=61, Evalue=7e-11,
Organism=Escherichia coli, GI1787570, Length=252, Percent_Identity=25.3968253968254, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 28957; Mature: 28826

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRSPALFLFALPALVVFLAFFCLPMARLIEVSLTGKDGAGVYWTVLTSGRYLHSLMVT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEECCCHHHHHHHHH
VLLSALVTLATLVIAGISGTFLQRHPVPGKPVLVAMLTFPLAFPGVVIGFMVIMLGGRNG
HHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LLASIGDALTGERWTFAYGLAGLFVGYLYFSIPRVILTVMAAVDKLDTSLEEAARSLGAS
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
RWHVVRDVMLPALMPAMLSSGAICFATSMGAFGTAFTLATQLDVLPLTIYNEFTNYANFG
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAAALSILLGAVTWMLLALARWYSGDAVAATA
HHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
>Mature Secondary Structure 
TRRSPALFLFALPALVVFLAFFCLPMARLIEVSLTGKDGAGVYWTVLTSGRYLHSLMVT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEECCCHHHHHHHHH
VLLSALVTLATLVIAGISGTFLQRHPVPGKPVLVAMLTFPLAFPGVVIGFMVIMLGGRNG
HHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LLASIGDALTGERWTFAYGLAGLFVGYLYFSIPRVILTVMAAVDKLDTSLEEAARSLGAS
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
RWHVVRDVMLPALMPAMLSSGAICFATSMGAFGTAFTLATQLDVLPLTIYNEFTNYANFG
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAAALSILLGAVTWMLLALARWYSGDAVAATA
HHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]