Click here to switch to the map view.

The map label for this gene is cysW [H]

Identifier: 94313764

GI number: 94313764

Start: 1499384

End: 1500259

Strand: Direct

Name: cysW [H]

Synonym: Rmet_4842

Alternate gene names: 94313764

Gene position: 1499384-1500259 (Clockwise)

Preceding gene: 94313763

Following gene: 94313765

Centisome position: 58.11

GC content: 64.38

Gene sequence:

>876_bases
ATGACTTCAAAACCCCGCCGCACCGCCTATTGGCTGCAACTGACACTCACACTCGCGGTCTGCGCGTTCATGACGGTGCC
CGTGGTGCTGTCCGTGCTGGCCGGCCTGACCAACAACATCTTTGTCGGCCTATCGAGCGGCCTGACTACCCGCTGGCTGG
TCGAAGTCTGGTCGCTCTACCGCAACACGATCTTTCTTTCGCTGGGTATCGCGCTGGCCTGTCTGGCCTGCACCCTCGTG
ATCGGCGTGCCGGCCGCGTACTACATGGCGCTGCGGCGCAACCGCGTGACCCGGCTGATCGAGGAACTGCTGATGCTGCC
CGTCGCCGTGCCTGGGCTGGCCACTGCACTGGGCTTGATCCTGCTTTACGGCGGCTGGCAGGCACTGCGGACGAGCTGGG
TGTTTATCCTGATCGGACACGTGCTGTTCACGCTGCCGTTCATGGTCCGATCGGTGCTGGCGATTCTCTCCGCCATTGAC
ATCCGCACGATCGAGGATGCTGCCGCGAGTCTGGGCGCCACGCGCCTGCAGCGCTTCTTCACGGTGGTGCTGCCCAATTG
CCGCCAGGGCATCCTGGCCGGCGCGCTGATGGTGGTGACGTTGTCGGTGGGCGAATTCAACCTGACGTGGATGCTCCATA
CGCCCACCACGCAAACGCTCCCGGTTGGGCTGGCCGACAGCTACGCGTCCATGCGGCTGGAAATCGGCTCGGCCTACACC
ATCGTCTTCTTCGTGATGATCATTCCGCTGCTGGTGTTCATGCAGGCGCTGAGCGCGTTGGGCGCGCGTGCCCGCCGCCA
CGCTCCCCCACCTGAAGCCACCGCAGAACTGCCGATGACTGCCGTACCCCAATCCGCCGAGACCCGCCATGCATGA

Upstream 100 bases:

>100_bases
GCGGCCTTGCCGTAACACTTGCAATGTCTGCGGCCGCGCGTCTAGCCAGCACCGCTTCGCTGCATCTTGATAGCGCCTCA
ATGCCTGCCGAGGATGCCCG

Downstream 100 bases:

>100_bases
GCCCACCGCAATCCGCCTGCGCCACTGCGCCAAGACCTTTGCCGATGGGACGACCGCCCTGCAGCCGCTCGACCTCGATA
TCGGCGCGGGGGAAACCGTG

Product: putative ABC transporter, inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTSKPRRTAYWLQLTLTLAVCAFMTVPVVLSVLAGLTNNIFVGLSSGLTTRWLVEVWSLYRNTIFLSLGIALACLACTLV
IGVPAAYYMALRRNRVTRLIEELLMLPVAVPGLATALGLILLYGGWQALRTSWVFILIGHVLFTLPFMVRSVLAILSAID
IRTIEDAAASLGATRLQRFFTVVLPNCRQGILAGALMVVTLSVGEFNLTWMLHTPTTQTLPVGLADSYASMRLEIGSAYT
IVFFVMIIPLLVFMQALSALGARARRHAPPPEATAELPMTAVPQSAETRHA

Sequences:

>Translated_291_residues
MTSKPRRTAYWLQLTLTLAVCAFMTVPVVLSVLAGLTNNIFVGLSSGLTTRWLVEVWSLYRNTIFLSLGIALACLACTLV
IGVPAAYYMALRRNRVTRLIEELLMLPVAVPGLATALGLILLYGGWQALRTSWVFILIGHVLFTLPFMVRSVLAILSAID
IRTIEDAAASLGATRLQRFFTVVLPNCRQGILAGALMVVTLSVGEFNLTWMLHTPTTQTLPVGLADSYASMRLEIGSAYT
IVFFVMIIPLLVFMQALSALGARARRHAPPPEATAELPMTAVPQSAETRHA
>Mature_290_residues
TSKPRRTAYWLQLTLTLAVCAFMTVPVVLSVLAGLTNNIFVGLSSGLTTRWLVEVWSLYRNTIFLSLGIALACLACTLVI
GVPAAYYMALRRNRVTRLIEELLMLPVAVPGLATALGLILLYGGWQALRTSWVFILIGHVLFTLPFMVRSVLAILSAIDI
RTIEDAAASLGATRLQRFFTVVLPNCRQGILAGALMVVTLSVGEFNLTWMLHTPTTQTLPVGLADSYASMRLEIGSAYTI
VFFVMIIPLLVFMQALSALGARARRHAPPPEATAELPMTAVPQSAETRHA

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=215, Percent_Identity=28.8372093023256, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31702; Mature: 31571

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSKPRRTAYWLQLTLTLAVCAFMTVPVVLSVLAGLTNNIFVGLSSGLTTRWLVEVWSLY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHH
RNTIFLSLGIALACLACTLVIGVPAAYYMALRRNRVTRLIEELLMLPVAVPGLATALGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LLYGGWQALRTSWVFILIGHVLFTLPFMVRSVLAILSAIDIRTIEDAAASLGATRLQRFF
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVVLPNCRQGILAGALMVVTLSVGEFNLTWMLHTPTTQTLPVGLADSYASMRLEIGSAYT
HHHCCHHHHHHHHHHHHHHHEECCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCHHH
IVFFVMIIPLLVFMQALSALGARARRHAPPPEATAELPMTAVPQSAETRHA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSKPRRTAYWLQLTLTLAVCAFMTVPVVLSVLAGLTNNIFVGLSSGLTTRWLVEVWSLY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHH
RNTIFLSLGIALACLACTLVIGVPAAYYMALRRNRVTRLIEELLMLPVAVPGLATALGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LLYGGWQALRTSWVFILIGHVLFTLPFMVRSVLAILSAIDIRTIEDAAASLGATRLQRFF
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVVLPNCRQGILAGALMVVTLSVGEFNLTWMLHTPTTQTLPVGLADSYASMRLEIGSAYT
HHHCCHHHHHHHHHHHHHHHEECCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCHHH
IVFFVMIIPLLVFMQALSALGARARRHAPPPEATAELPMTAVPQSAETRHA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]