BacMap

Definition	Cupriavidus metallidurans CH34 megaplasmid, complete sequence.
Accession	NC_007974
Length	2,580,084

Click here to switch to the map view.

The map label for this gene is garP [H]

Identifier: 94313657

GI number: 94313657

Start: 1378805

End: 1380166

Strand: Reverse

Name: garP [H]

Synonym: Rmet_4735

Alternate gene names: 94313657

Gene position: 1380166-1378805 (Counterclockwise)

Preceding gene: 94313659

Following gene: 94313656

Centisome position: 53.49

GC content: 62.56

Gene sequence:

>1362_bases
ATGAATGCCACCCCCACCACAGGCGCTGCAGCCGCATCCACCGCGCGGCGCACCAATGTCCGTTACTGGATTCTCGCGCT
GATTTTCATCGTGACCACGGTCAACTACGCGGATCGTGCCACGCTGTCGATTACCGGGCCCGCGATGCGCGAGGAATTCG
GTTTCTCGGCCGTCCAGATGGGCTATATCTTCTCGGCCTTCAGTTGGTCGTACGTGATGGCGCAGATTCCTGGCGGCTGG
CTACTCGATCGCTTTGGCGCCCGGCGCATCTATGCCGTGAGCATCTTCCTCTGGTCATTCTTCACGCTGCTGCAGGGGTG
GGTCGGCATCTTCGCCTCGATGGGAGCAACGATGACGGCGCTCTTCATGCTGCGTTTCGCCGTGGGCCTGGCCGAGTCCC
CGGCATTCCCGGCCAATGCAAAGGTGGTGGCAAGCTGGTTCCCGACCGCCGAGCGCGGCACCGCGTCGGCCATCTTCAAC
GCCGCGCAGTACTTCGCCGCCGTGGTGTTCACGCCGCTGATGGCATGGATCGTTCACAGCATGGGCTGGCATCACGTCTA
CCTGTGGATGGGCATCATGGGCATGCTGCTGGCGATGCTCTGGCTCAAGGTCATGCGCAGCCCGGTCACGCATCCGGCTG
TGAATCGCGCGGAACTTGAGCACATCGAGCAGGGCGGTGGCCTGATTCACATGAATGAAGGGGCAGGCAAGTCAAAGCAG
GGCGCTAATCCCGCTGGCTGGTTCTATGCCCGGCAGTTGCTGTCGAACCGGATGCTGGCCGGGGTCTATCTGGGCCAGTA
CTGCATCAACGTGCTGACGTACTTCTTCCTCACGTGGTTTCCGGTGTACCTGGTGCAAGCGCGCGGCATGTCGATCCTGA
AGGCGGGTTTCGTCGCGTCCCTGCCGGCCATCTGCGGTTTTCTCGGCGGCGTGCTGGGCGGCGTGATCTCCGACCACTTG
CTGCGCCGCGGCTACTCACTGACCATCGCCCGCAAGGTGCCGATCGTGGCGGGCATGTTGCTGTCGGTGTCCATGATCGC
CTGCAACTACGTGGATGCCGAATGGCTGGTGGTGGGCATCATGGCGCTCGCGTTCTTCGGCAAGGGCATCGGCGCGCTGG
GCTGGGCCGTGGTGGCTGACACTGCGCCCAAGGAAGTGATCGGCCTGGCGGGCAGCATCTTCAACACGTTCGGCAATATC
GCCGGCATTGTGACGCCGATCGTGATCGGCTATATCCTGAGCGCGACAGGCTCGTTCAACGGCGCGCTGGTGTTCGTGGG
TATCAATGCCCTGGTCACCGTGCTGAGCTATCTGGTGATCGTCAAGGACATTCGCCGCGTCGAGCTGAAGCACCCCCAAT
AA

Upstream 100 bases:

>100_bases
TCGAGCCTCGTTCTTATAATTCAAGTCGTATGACGACATATCTCAAGCCGGTCCAGAAGCACTCCCGCATCCAGGAACCA
GGGCTGTGAGGAGACCTCTC

Downstream 100 bases:

>100_bases
GGAAGGAATCCCCAACATGTCGGACTTGTCGGTACAAAACGCGCAGGGCCAGGCCGCCGAACGCGCGCCGCTCTACATCC
GCATTCACGCCAATGACAAC

Product: Galactarate transporter

Products: NA

Alternate protein names: D-glucarate permease [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MNATPTTGAAAASTARRTNVRYWILALIFIVTTVNYADRATLSITGPAMREEFGFSAVQMGYIFSAFSWSYVMAQIPGGW
LLDRFGARRIYAVSIFLWSFFTLLQGWVGIFASMGATMTALFMLRFAVGLAESPAFPANAKVVASWFPTAERGTASAIFN
AAQYFAAVVFTPLMAWIVHSMGWHHVYLWMGIMGMLLAMLWLKVMRSPVTHPAVNRAELEHIEQGGGLIHMNEGAGKSKQ
GANPAGWFYARQLLSNRMLAGVYLGQYCINVLTYFFLTWFPVYLVQARGMSILKAGFVASLPAICGFLGGVLGGVISDHL
LRRGYSLTIARKVPIVAGMLLSVSMIACNYVDAEWLVVGIMALAFFGKGIGALGWAVVADTAPKEVIGLAGSIFNTFGNI
AGIVTPIVIGYILSATGSFNGALVFVGINALVTVLSYLVIVKDIRRVELKHPQ

Sequences:

>Translated_453_residues
MNATPTTGAAAASTARRTNVRYWILALIFIVTTVNYADRATLSITGPAMREEFGFSAVQMGYIFSAFSWSYVMAQIPGGW
LLDRFGARRIYAVSIFLWSFFTLLQGWVGIFASMGATMTALFMLRFAVGLAESPAFPANAKVVASWFPTAERGTASAIFN
AAQYFAAVVFTPLMAWIVHSMGWHHVYLWMGIMGMLLAMLWLKVMRSPVTHPAVNRAELEHIEQGGGLIHMNEGAGKSKQ
GANPAGWFYARQLLSNRMLAGVYLGQYCINVLTYFFLTWFPVYLVQARGMSILKAGFVASLPAICGFLGGVLGGVISDHL
LRRGYSLTIARKVPIVAGMLLSVSMIACNYVDAEWLVVGIMALAFFGKGIGALGWAVVADTAPKEVIGLAGSIFNTFGNI
AGIVTPIVIGYILSATGSFNGALVFVGINALVTVLSYLVIVKDIRRVELKHPQ
>Mature_453_residues
MNATPTTGAAAASTARRTNVRYWILALIFIVTTVNYADRATLSITGPAMREEFGFSAVQMGYIFSAFSWSYVMAQIPGGW
LLDRFGARRIYAVSIFLWSFFTLLQGWVGIFASMGATMTALFMLRFAVGLAESPAFPANAKVVASWFPTAERGTASAIFN
AAQYFAAVVFTPLMAWIVHSMGWHHVYLWMGIMGMLLAMLWLKVMRSPVTHPAVNRAELEHIEQGGGLIHMNEGAGKSKQ
GANPAGWFYARQLLSNRMLAGVYLGQYCINVLTYFFLTWFPVYLVQARGMSILKAGFVASLPAICGFLGGVLGGVISDHL
LRRGYSLTIARKVPIVAGMLLSVSMIACNYVDAEWLVVGIMALAFFGKGIGALGWAVVADTAPKEVIGLAGSIFNTFGNI
AGIVTPIVIGYILSATGSFNGALVFVGINALVTVLSYLVIVKDIRRVELKHPQ

Specific function: Uptake of D-glucarate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Phthalate permease family [H]

Homologues:

Organism=Homo sapiens, GI9945322, Length=368, Percent_Identity=26.9021739130435, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI9966811, Length=411, Percent_Identity=26.5206812652068, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI21322234, Length=395, Percent_Identity=25.8227848101266, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI148743791, Length=385, Percent_Identity=24.6753246753247, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI6912666, Length=367, Percent_Identity=26.4305177111717, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI4885441, Length=390, Percent_Identity=23.3333333333333, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI223718438, Length=395, Percent_Identity=24.3037974683544, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI5031955, Length=327, Percent_Identity=23.8532110091743, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI154350196, Length=421, Percent_Identity=23.0403800475059, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI148728191, Length=311, Percent_Identity=24.1157556270096, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI256219598, Length=393, Percent_Identity=22.3918575063613, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI4503847, Length=393, Percent_Identity=22.3918575063613, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI256219483, Length=393, Percent_Identity=22.3918575063613, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI256219543, Length=304, Percent_Identity=23.6842105263158, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1789152, Length=441, Percent_Identity=64.172335600907, Blast_Score=600, Evalue=1e-173,
Organism=Escherichia coli, GI1789515, Length=439, Percent_Identity=56.4920273348519, Blast_Score=527, Evalue=1e-151,
Organism=Escherichia coli, GI87082320, Length=438, Percent_Identity=40.8675799086758, Blast_Score=352, Evalue=4e-98,
Organism=Escherichia coli, GI2367379, Length=441, Percent_Identity=29.7052154195011, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI2367193, Length=325, Percent_Identity=27.6923076923077, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1788573, Length=447, Percent_Identity=22.5950782997763, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI115533556, Length=435, Percent_Identity=26.4367816091954, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI115533554, Length=394, Percent_Identity=26.3959390862944, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17552542, Length=373, Percent_Identity=25.4691689008043, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17535049, Length=415, Percent_Identity=23.1325301204819, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17560030, Length=365, Percent_Identity=24.6575342465753, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI193205400, Length=297, Percent_Identity=22.8956228956229, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71997452, Length=386, Percent_Identity=23.3160621761658, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI32565916, Length=270, Percent_Identity=21.1111111111111, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI32565914, Length=270, Percent_Identity=21.1111111111111, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17554148, Length=365, Percent_Identity=23.8356164383562, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI193208223, Length=344, Percent_Identity=24.1279069767442, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71991448, Length=411, Percent_Identity=20.6812652068127, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71991453, Length=411, Percent_Identity=20.6812652068127, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71983953, Length=272, Percent_Identity=23.1617647058824, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI193207426, Length=285, Percent_Identity=23.1578947368421, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17562692, Length=318, Percent_Identity=22.3270440251572, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17537329, Length=275, Percent_Identity=23.2727272727273, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25141316, Length=368, Percent_Identity=22.0108695652174, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25141318, Length=368, Percent_Identity=22.0108695652174, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17554962, Length=367, Percent_Identity=22.0708446866485, Blast_Score=66, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI25145964, Length=273, Percent_Identity=22.3443223443223, Blast_Score=66, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17569727, Length=403, Percent_Identity=21.8362282878412, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI45550460, Length=421, Percent_Identity=27.5534441805226, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24641682, Length=394, Percent_Identity=26.1421319796954, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24639764, Length=398, Percent_Identity=27.1356783919598, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24583789, Length=418, Percent_Identity=25.1196172248804, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24655255, Length=373, Percent_Identity=27.6139410187668, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24584153, Length=420, Percent_Identity=25.2380952380952, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648416, Length=386, Percent_Identity=23.8341968911917, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21356189, Length=386, Percent_Identity=23.8341968911917, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24654215, Length=375, Percent_Identity=25.6, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI19922400, Length=375, Percent_Identity=25.3333333333333, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28573299, Length=419, Percent_Identity=25.7756563245823, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI62484226, Length=373, Percent_Identity=24.6648793565684, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI28573708, Length=378, Percent_Identity=25.6613756613757, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28573707, Length=378, Percent_Identity=25.6613756613757, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI20130167, Length=412, Percent_Identity=23.0582524271845, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24655271, Length=412, Percent_Identity=23.0582524271845, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI20130135, Length=412, Percent_Identity=23.0582524271845, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20129235, Length=403, Percent_Identity=26.0545905707196, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24658658, Length=429, Percent_Identity=24.2424242424242, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24580790, Length=409, Percent_Identity=23.2273838630807, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45550364, Length=440, Percent_Identity=24.0909090909091, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24653727, Length=382, Percent_Identity=23.2984293193717, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24658674, Length=419, Percent_Identity=23.6276849642005, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19920686, Length=410, Percent_Identity=23.1707317073171, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24658666, Length=415, Percent_Identity=23.3734939759036, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24658696, Length=435, Percent_Identity=24.367816091954, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004744
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49097; Mature: 49097

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATPTTGAAAASTARRTNVRYWILALIFIVTTVNYADRATLSITGPAMREEFGFSAVQM
CCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHCCHHHHH
GYIFSAFSWSYVMAQIPGGWLLDRFGARRIYAVSIFLWSFFTLLQGWVGIFASMGATMTA
HHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFMLRFAVGLAESPAFPANAKVVASWFPTAERGTASAIFNAAQYFAAVVFTPLMAWIVHS
HHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGWHHVYLWMGIMGMLLAMLWLKVMRSPVTHPAVNRAELEHIEQGGGLIHMNEGAGKSKQ
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCC
GANPAGWFYARQLLSNRMLAGVYLGQYCINVLTYFFLTWFPVYLVQARGMSILKAGFVAS
CCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LPAICGFLGGVLGGVISDHLLRRGYSLTIARKVPIVAGMLLSVSMIACNYVDAEWLVVGI
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
MALAFFGKGIGALGWAVVADTAPKEVIGLAGSIFNTFGNIAGIVTPIVIGYILSATGSFN
HHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GALVFVGINALVTVLSYLVIVKDIRRVELKHPQ
CEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNATPTTGAAAASTARRTNVRYWILALIFIVTTVNYADRATLSITGPAMREEFGFSAVQM
CCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHCCHHHHH
GYIFSAFSWSYVMAQIPGGWLLDRFGARRIYAVSIFLWSFFTLLQGWVGIFASMGATMTA
HHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFMLRFAVGLAESPAFPANAKVVASWFPTAERGTASAIFNAAQYFAAVVFTPLMAWIVHS
HHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGWHHVYLWMGIMGMLLAMLWLKVMRSPVTHPAVNRAELEHIEQGGGLIHMNEGAGKSKQ
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCC
GANPAGWFYARQLLSNRMLAGVYLGQYCINVLTYFFLTWFPVYLVQARGMSILKAGFVAS
CCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LPAICGFLGGVLGGVISDHLLRRGYSLTIARKVPIVAGMLLSVSMIACNYVDAEWLVVGI
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
MALAFFGKGIGALGWAVVADTAPKEVIGLAGSIFNTFGNIAGIVTPIVIGYILSATGSFN
HHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GALVFVGINALVTVLSYLVIVKDIRRVELKHPQ
CEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7704254; 9384377 [H]