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The map label for this gene is hyfF [H]

Identifier: 94313590

GI number: 94313590

Start: 1300070

End: 1301524

Strand: Reverse

Name: hyfF [H]

Synonym: Rmet_4668

Alternate gene names: 94313590

Gene position: 1301524-1300070 (Counterclockwise)

Preceding gene: 94313591

Following gene: 94313589

Centisome position: 50.45

GC content: 61.44

Gene sequence:

>1455_bases
GTGGCGGCGCTCGCATTCCTGCTCGCAATTCCGCTCGCCGGCGGCCTGGTGCTGGCACTGGCAGGGCATCGCGAACATGC
GCGCGACATCAATGTTGCGTTCAGCCTGGGCACCTTTGCTGCGGCCTGCGTGCTCGCGGCGATTGTTGTCTCCGATGGCC
CCTTGCTCGCGTGGCAGTACGAGTTCTACGTCGACTCTCTGAACGTCTTCCTGGTCGCGCTGACCGCCCTCGTGGGGCTG
ACTACTTCGCTCTTTTCGCGGCCATATATGCGGATCGAACGTGACCACGGCAAGATGACACCGCCCCGTCTGCGGCTGTA
CCACAGCATGTATCAGTTGTTCATGTTCACCATGTTGCTGGCGCTGACCACAAACAATCTCGGCATCGTGTGGGTCGCAA
TGGAAGCCGCAACGCTGACAACAGTGCTATTGGTCAGCGTCTACCGCACCGCGGCCAGCCTGGAGGCAGCGTGGAAATAT
TTCATTCTGTGCGGTGTGGGTATCGCTCAGGCGCTGTTTGGCACGGTGCTGCTCTACATGGCGGCGGAGAAGGTGGTCGG
TGCCGAGGGCGGCGCTCTGCTATGGACCAACCTCAACGCGGTAAAGGAACACCTGGACCCCAATATCATCACACTGGCCT
TCGCCTTTCTCTTCATCGGCTATGGCACCAAGGTGGGGCTGGTGCCACTGCACAACTGGCTACCCGATGCGCACGCAGAG
GGGCCGACGCCGGTGTCAGCCGTGCTGTCCGGCCTGCTGCTTAATGTGGCCCTCTACGCGGTGCTGCGTTGCAAGATGCT
GACGGACGCGGCGGTGGGCAGCCATCTCACCGGCTATCTCATGATGGGCTTCGGTCTGCTCTCAGTGGTGGCGGCCGTGT
TTTTCCTGATGCGTCAGCGCGACGTAAAGCGCATGTTTGCGTACTCGTCGATCGAGCATATGGGCCTGATCACCTTTGCC
TTTGGCATGGGCGGTCCGATCGCCAGCTATGCCGGGCTGCTGCACATGACGATGCATTCGCTGGTCAAGTCAGCCATCTT
TTTCGCCGTCGGCCATGCCGCGCAGAAAGCAGGCACGCAGATCATGGATGACATCCGCGGGCTGATCCGCGTCAGTCCCA
CGGTTGGTTGGGGCTTGATGCTGGGCGCGCTGGCCATCCTCGGCATGCCACCGTTCGGCGTCTTTGCCAGCGAGTTCCTG
ATTGTCACGACCGCCATGCGCGAGCATGCATGGGCGACGCCGTTTCTGTTGATCGCGCTCGGCGTGGCCTTCGCATCGAT
CTTCAGCCGTGTTCAGCCGATGGTGTTCGGTGACACCGAACTCAAGCCGCTGGCGCATCCACCGGCCTTGGTCCCCGTGT
TCCTGCATCTTGCGCTTGGGCTGGTTGCAGGACTTTACATTCCGCCATATCTCGATGTCTGGTACCGGCAGGCAGCCGCG
ATGCTGGGAGGCTAA

Upstream 100 bases:

>100_bases
CTGATCGGGGTGCTGATCCTGGGCGTGTTCATGTTCCAGATCCGGGCGCAGTTCGACAGTCTGGACATCCGGAATCTGGA
GCAATTGAAGGAGGATTGAC

Downstream 100 bases:

>100_bases
GCGCATGACGCAACCCGACCTTGGTGTAGAGCTGGAGCGCCTGGTGGCGCCATTGCCGATGTGGCACGGGCGCCTTGGTC
AAGATGCCTGGCAGGCGATT

Product: hydrogenase 4 subunit F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MAALAFLLAIPLAGGLVLALAGHREHARDINVAFSLGTFAAACVLAAIVVSDGPLLAWQYEFYVDSLNVFLVALTALVGL
TTSLFSRPYMRIERDHGKMTPPRLRLYHSMYQLFMFTMLLALTTNNLGIVWVAMEAATLTTVLLVSVYRTAASLEAAWKY
FILCGVGIAQALFGTVLLYMAAEKVVGAEGGALLWTNLNAVKEHLDPNIITLAFAFLFIGYGTKVGLVPLHNWLPDAHAE
GPTPVSAVLSGLLLNVALYAVLRCKMLTDAAVGSHLTGYLMMGFGLLSVVAAVFFLMRQRDVKRMFAYSSIEHMGLITFA
FGMGGPIASYAGLLHMTMHSLVKSAIFFAVGHAAQKAGTQIMDDIRGLIRVSPTVGWGLMLGALAILGMPPFGVFASEFL
IVTTAMREHAWATPFLLIALGVAFASIFSRVQPMVFGDTELKPLAHPPALVPVFLHLALGLVAGLYIPPYLDVWYRQAAA
MLGG

Sequences:

>Translated_484_residues
MAALAFLLAIPLAGGLVLALAGHREHARDINVAFSLGTFAAACVLAAIVVSDGPLLAWQYEFYVDSLNVFLVALTALVGL
TTSLFSRPYMRIERDHGKMTPPRLRLYHSMYQLFMFTMLLALTTNNLGIVWVAMEAATLTTVLLVSVYRTAASLEAAWKY
FILCGVGIAQALFGTVLLYMAAEKVVGAEGGALLWTNLNAVKEHLDPNIITLAFAFLFIGYGTKVGLVPLHNWLPDAHAE
GPTPVSAVLSGLLLNVALYAVLRCKMLTDAAVGSHLTGYLMMGFGLLSVVAAVFFLMRQRDVKRMFAYSSIEHMGLITFA
FGMGGPIASYAGLLHMTMHSLVKSAIFFAVGHAAQKAGTQIMDDIRGLIRVSPTVGWGLMLGALAILGMPPFGVFASEFL
IVTTAMREHAWATPFLLIALGVAFASIFSRVQPMVFGDTELKPLAHPPALVPVFLHLALGLVAGLYIPPYLDVWYRQAAA
MLGG
>Mature_483_residues
AALAFLLAIPLAGGLVLALAGHREHARDINVAFSLGTFAAACVLAAIVVSDGPLLAWQYEFYVDSLNVFLVALTALVGLT
TSLFSRPYMRIERDHGKMTPPRLRLYHSMYQLFMFTMLLALTTNNLGIVWVAMEAATLTTVLLVSVYRTAASLEAAWKYF
ILCGVGIAQALFGTVLLYMAAEKVVGAEGGALLWTNLNAVKEHLDPNIITLAFAFLFIGYGTKVGLVPLHNWLPDAHAEG
PTPVSAVLSGLLLNVALYAVLRCKMLTDAAVGSHLTGYLMMGFGLLSVVAAVFFLMRQRDVKRMFAYSSIEHMGLITFAF
GMGGPIASYAGLLHMTMHSLVKSAIFFAVGHAAQKAGTQIMDDIRGLIRVSPTVGWGLMLGALAILGMPPFGVFASEFLI
VTTAMREHAWATPFLLIALGVAFASIFSRVQPMVFGDTELKPLAHPPALVPVFLHLALGLVAGLYIPPYLDVWYRQAAAM
LGG

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=381, Percent_Identity=26.509186351706, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI251831116, Length=348, Percent_Identity=24.4252873563218, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1788831, Length=387, Percent_Identity=43.1524547803618, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI1788827, Length=348, Percent_Identity=29.5977011494253, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1788613, Length=379, Percent_Identity=26.3852242744063, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788829, Length=408, Percent_Identity=25, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788614, Length=421, Percent_Identity=24.9406175771971, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI145693160, Length=367, Percent_Identity=22.8882833787466, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI2367154, Length=375, Percent_Identity=23.7333333333333, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 52097; Mature: 51966

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALAFLLAIPLAGGLVLALAGHREHARDINVAFSLGTFAAACVLAAIVVSDGPLLAWQY
CHHHHHHHHHHHHCHHHEEECCCHHHHCHHHHEEEHHHHHHHHHHHHHHHCCCCEEEEEH
EFYVDSLNVFLVALTALVGLTTSLFSRPYMRIERDHGKMTPPRLRLYHSMYQLFMFTMLL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
ALTTNNLGIVWVAMEAATLTTVLLVSVYRTAASLEAAWKYFILCGVGIAQALFGTVLLYM
HHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAEKVVGAEGGALLWTNLNAVKEHLDPNIITLAFAFLFIGYGTKVGLVPLHNWLPDAHAE
HHHHHHCCCCCEEEEECHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEECHHCCCCCCCC
GPTPVSAVLSGLLLNVALYAVLRCKMLTDAAVGSHLTGYLMMGFGLLSVVAAVFFLMRQR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVKRMFAYSSIEHMGLITFAFGMGGPIASYAGLLHMTMHSLVKSAIFFAVGHAAQKAGTQ
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IMDDIRGLIRVSPTVGWGLMLGALAILGMPPFGVFASEFLIVTTAMREHAWATPFLLIAL
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
GVAFASIFSRVQPMVFGDTELKPLAHPPALVPVFLHLALGLVAGLYIPPYLDVWYRQAAA
HHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
MLGG
HHCC
>Mature Secondary Structure 
AALAFLLAIPLAGGLVLALAGHREHARDINVAFSLGTFAAACVLAAIVVSDGPLLAWQY
HHHHHHHHHHHHCHHHEEECCCHHHHCHHHHEEEHHHHHHHHHHHHHHHCCCCEEEEEH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HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA