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The map label for this gene is hyfE [C]

Identifier: 94313591

GI number: 94313591

Start: 1301526

End: 1302185

Strand: Reverse

Name: hyfE [C]

Synonym: Rmet_4669

Alternate gene names: 94313591

Gene position: 1302185-1301526 (Counterclockwise)

Preceding gene: 94313592

Following gene: 94313590

Centisome position: 50.47

GC content: 57.73

Gene sequence:

>660_bases
ATGAGCATGCTACTCGCCCAGTTTGTCAACCTGCTCGGCGCGGTCCTGCTGATACTCGCGTTTGCGATGATTTCGCAGCG
CCGCATCCTGTCACTGATTCATCTGTTTACGCTGCAGGGCATGACATTGGCGCTGGCGACAGCGGTGGTTGGCTATGTCA
CAACCCAGCCGCACCTGTACCTGTCGGCCGCGCTGACGCTGGTGCTCAAGGCCCTGTTGATTCCCTATCTGCTGCACCGG
ATCATCGATCGGTTGCAGATTCGTTGGGACATAGAAAGGCTGATCAATATTCCCACCACCATGCTGATCGGCATTCTCGT
GGTGATATTCGCCTTCAACCTGGCCATGCCGATCTCGCAAATGTCGCTCAAGCTGGCCAGCGGCACGCTCGGCATTGCGC
TGGCGTGCGTGCTGCTGTCCTTCCTGATGATGATCACACGCTCCAAGGCGGTGCCACAGGTGATCGGCTTTCTGTCGATG
GAAAACGGGCTGTTCTTTGCAGCCACCGCAGCCACCTACGGCATGCCGATGGTGGTTGAGCTGGGCATTGCGCTGGATGT
GCTGATCGGGGTGCTGATCCTGGGCGTGTTCATGTTCCAGATCCGGGCGCAGTTCGACAGTCTGGACATCCGGAATCTGG
AGCAATTGAAGGAGGATTGA

Upstream 100 bases:

>100_bases
AACGCCAAGATGCGGATATTCCGTGTACCCGAGTTCCTCGCCACCGCTTTCCTGCTGGCGGTCATCGGCCTGCTTGTGCA
TCTGCTCCTGGTCCACTGAT

Downstream 100 bases:

>100_bases
CGTGGCGGCGCTCGCATTCCTGCTCGCAATTCCGCTCGCCGGCGGCCTGGTGCTGGCACTGGCAGGGCATCGCGAACATG
CGCGCGACATCAATGTTGCG

Product: Hydrogenase 4 subunit E, membrane component

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MSMLLAQFVNLLGAVLLILAFAMISQRRILSLIHLFTLQGMTLALATAVVGYVTTQPHLYLSAALTLVLKALLIPYLLHR
IIDRLQIRWDIERLINIPTTMLIGILVVIFAFNLAMPISQMSLKLASGTLGIALACVLLSFLMMITRSKAVPQVIGFLSM
ENGLFFAATAATYGMPMVVELGIALDVLIGVLILGVFMFQIRAQFDSLDIRNLEQLKED

Sequences:

>Translated_219_residues
MSMLLAQFVNLLGAVLLILAFAMISQRRILSLIHLFTLQGMTLALATAVVGYVTTQPHLYLSAALTLVLKALLIPYLLHR
IIDRLQIRWDIERLINIPTTMLIGILVVIFAFNLAMPISQMSLKLASGTLGIALACVLLSFLMMITRSKAVPQVIGFLSM
ENGLFFAATAATYGMPMVVELGIALDVLIGVLILGVFMFQIRAQFDSLDIRNLEQLKED
>Mature_218_residues
SMLLAQFVNLLGAVLLILAFAMISQRRILSLIHLFTLQGMTLALATAVVGYVTTQPHLYLSAALTLVLKALLIPYLLHRI
IDRLQIRWDIERLINIPTTMLIGILVVIFAFNLAMPISQMSLKLASGTLGIALACVLLSFLMMITRSKAVPQVIGFLSME
NGLFFAATAATYGMPMVVELGIALDVLIGVLILGVFMFQIRAQFDSLDIRNLEQLKED

Specific function: Unknown

COG id: COG4237

COG function: function code C; Hydrogenase 4 membrane component (E)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.-.-.- [C]

Molecular weight: Translated: 24120; Mature: 23989

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMLLAQFVNLLGAVLLILAFAMISQRRILSLIHLFTLQGMTLALATAVVGYVTTQPHLY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LSAALTLVLKALLIPYLLHRIIDRLQIRWDIERLINIPTTMLIGILVVIFAFNLAMPISQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHH
MSLKLASGTLGIALACVLLSFLMMITRSKAVPQVIGFLSMENGLFFAATAATYGMPMVVE
HHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEHHHHHCCHHHHH
LGIALDVLIGVLILGVFMFQIRAQFDSLDIRNLEQLKED
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCC
>Mature Secondary Structure 
SMLLAQFVNLLGAVLLILAFAMISQRRILSLIHLFTLQGMTLALATAVVGYVTTQPHLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LSAALTLVLKALLIPYLLHRIIDRLQIRWDIERLINIPTTMLIGILVVIFAFNLAMPISQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHH
MSLKLASGTLGIALACVLLSFLMMITRSKAVPQVIGFLSMENGLFFAATAATYGMPMVVE
HHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEHHHHHCCHHHHH
LGIALDVLIGVLILGVFMFQIRAQFDSLDIRNLEQLKED
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]