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The map label for this gene is hyfG [H]

Identifier: 94313589

GI number: 94313589

Start: 1298521

End: 1300065

Strand: Reverse

Name: hyfG [H]

Synonym: Rmet_4667

Alternate gene names: 94313589

Gene position: 1300065-1298521 (Counterclockwise)

Preceding gene: 94313590

Following gene: 94313588

Centisome position: 50.39

GC content: 64.6

Gene sequence:

>1545_bases
ATGACGCAACCCGACCTTGGTGTAGAGCTGGAGCGCCTGGTGGCGCCATTGCCGATGTGGCACGGGCGCCTTGGTCAAGA
TGCCTGGCAGGCGATTGCGTGTGTCGTTGCGAACGCGGGCGGACGCCTTGTTGCTGTGTGGGGCGCCGAGCGCGATCCAG
ATGCGATGGCGACGGCTTGCGTTGCCTATGCGATGCCGCATGGACTGGTCTGGCTCGAACTTCAGCTCGACGGCGCCCGG
GCGATACCGGATCTGGCTGGCGTCTTTCCCTGTGCCAGCCGGATGCAACGAGCTATGACCGACCTCTCTGGAGTTTCCGT
GGAAGGCGCCCACGATACTCGTCCGTGGCTTGATCATGGCCTGTGGCCGCCTGGCGCGCGTCCTCTGCAGTCAAGTGGGC
CGGGGTTCGCCGTCGCAGCAGGGATGTTGCCGGCGGACTACGCCTTCTTGCGCGTTGAGGGTGATGGCGTACACGAGGTC
GCGGTAGGGCCGGTACACGCCGGCATCATCGAGCCCGGACACTTCCGCTTCTCGGTGGTTGGCGAAAAAGTATTGCGGCT
CGAGGAACGGCTGGGCTATACGCACAAGGGAATCGAGCGTCGGTTTACTGAGCTTGCGCCAGAGGAGGGCTATCGGTTGG
CGGGCCGGGTCTCGGGCGATTCCACGGTGGCTTACGCCTGGGCCTACTGCATGGCGCTCGAATCCGCATGCCGCATGACG
CTGCCTGCGCGCGCGGCCTGCTTACGTGCGCTGATGCTCGAGCGCGAACGGGTGGCCAATCACCTGGGCGATCTTGGCGC
ACTGGGCAATGATGCGGGGCTGGCATTCGGATTAGCGCAGTTCTCGCGCTTGCGCGAAGACTGGCAGCGACTGTCGGGGC
AAGTGTTTGGCCATCGACTGATGATGGATGTGGTGGTGCCAGGCGGGGTGGCGATCGACACGTCTGCGCCCGCGCTGGAG
CGGCTGGGGCGGCAGTGTGACGAAATTGAGCGCGAACTGCACGTGCTGCGTGCCATCTACGATGAGCATGCCGGTTTGCA
GGACCGCTTTCGGGGTACCGGGCAGATAACCCCGCAGCTCGCGGCCCGGCTCGGACTCACCGGACTGGCAGGTCGTGCGA
GCGGCCAGGCCGTGGATCTGCGCTGCGACGTCGCATGGCTGTCCTATGAAACCCTGGCGGTCAGAATGGCCACGCAGAAC
GTTGGCGATGTGGCGGCGCGCGTGGCGGTGCGCTTTGACGAGGTGATTGAATCGCTGCGACTTATTCGTGCCCTCTGCAC
CGGGCTGCCAGAGGGGGAAATTCGCGGCGAACTGCGGTTACCGAGCGTGAGGGCGATCGGAGCAGGGTGGGTGGAAGGGT
GGCGCGGCGAGGTCCTCGTCGCGCTCGAACTCGGGGCCGACGACCGTGTTCTGCGTTGTCATTGTCATGACCCTTCATGG
CAGAACTGGCCGGCGCTCGAGCATGCCATTATCGGCAACATCGTTCCGGACTTTCCGCTGATCAACAAGTCCTTCAACCT
CAGCTATGCGGGGCACGACCTCTGA

Upstream 100 bases:

>100_bases
TTCCTGCATCTTGCGCTTGGGCTGGTTGCAGGACTTTACATTCCGCCATATCTCGATGTCTGGTACCGGCAGGCAGCCGC
GATGCTGGGAGGCTAAGCGC

Downstream 100 bases:

>100_bases
TGTGGCAAATACTGAAGCAGATCGCGCGTACCGGCATTCTCACGGAGCCTGCGCCGCAAGGTAGTGCCGACGACACCGCG
ATCGAGCGTATCCACAAGGA

Product: Hydrogenase-4 component G

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 5; Hydrogenase-3 component E [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MTQPDLGVELERLVAPLPMWHGRLGQDAWQAIACVVANAGGRLVAVWGAERDPDAMATACVAYAMPHGLVWLELQLDGAR
AIPDLAGVFPCASRMQRAMTDLSGVSVEGAHDTRPWLDHGLWPPGARPLQSSGPGFAVAAGMLPADYAFLRVEGDGVHEV
AVGPVHAGIIEPGHFRFSVVGEKVLRLEERLGYTHKGIERRFTELAPEEGYRLAGRVSGDSTVAYAWAYCMALESACRMT
LPARAACLRALMLERERVANHLGDLGALGNDAGLAFGLAQFSRLREDWQRLSGQVFGHRLMMDVVVPGGVAIDTSAPALE
RLGRQCDEIERELHVLRAIYDEHAGLQDRFRGTGQITPQLAARLGLTGLAGRASGQAVDLRCDVAWLSYETLAVRMATQN
VGDVAARVAVRFDEVIESLRLIRALCTGLPEGEIRGELRLPSVRAIGAGWVEGWRGEVLVALELGADDRVLRCHCHDPSW
QNWPALEHAIIGNIVPDFPLINKSFNLSYAGHDL

Sequences:

>Translated_514_residues
MTQPDLGVELERLVAPLPMWHGRLGQDAWQAIACVVANAGGRLVAVWGAERDPDAMATACVAYAMPHGLVWLELQLDGAR
AIPDLAGVFPCASRMQRAMTDLSGVSVEGAHDTRPWLDHGLWPPGARPLQSSGPGFAVAAGMLPADYAFLRVEGDGVHEV
AVGPVHAGIIEPGHFRFSVVGEKVLRLEERLGYTHKGIERRFTELAPEEGYRLAGRVSGDSTVAYAWAYCMALESACRMT
LPARAACLRALMLERERVANHLGDLGALGNDAGLAFGLAQFSRLREDWQRLSGQVFGHRLMMDVVVPGGVAIDTSAPALE
RLGRQCDEIERELHVLRAIYDEHAGLQDRFRGTGQITPQLAARLGLTGLAGRASGQAVDLRCDVAWLSYETLAVRMATQN
VGDVAARVAVRFDEVIESLRLIRALCTGLPEGEIRGELRLPSVRAIGAGWVEGWRGEVLVALELGADDRVLRCHCHDPSW
QNWPALEHAIIGNIVPDFPLINKSFNLSYAGHDL
>Mature_513_residues
TQPDLGVELERLVAPLPMWHGRLGQDAWQAIACVVANAGGRLVAVWGAERDPDAMATACVAYAMPHGLVWLELQLDGARA
IPDLAGVFPCASRMQRAMTDLSGVSVEGAHDTRPWLDHGLWPPGARPLQSSGPGFAVAAGMLPADYAFLRVEGDGVHEVA
VGPVHAGIIEPGHFRFSVVGEKVLRLEERLGYTHKGIERRFTELAPEEGYRLAGRVSGDSTVAYAWAYCMALESACRMTL
PARAACLRALMLERERVANHLGDLGALGNDAGLAFGLAQFSRLREDWQRLSGQVFGHRLMMDVVVPGGVAIDTSAPALER
LGRQCDEIERELHVLRAIYDEHAGLQDRFRGTGQITPQLAARLGLTGLAGRASGQAVDLRCDVAWLSYETLAVRMATQNV
GDVAARVAVRFDEVIESLRLIRALCTGLPEGEIRGELRLPSVRAIGAGWVEGWRGEVLVALELGADDRVLRCHCHDPSWQ
NWPALEHAIIGNIVPDFPLINKSFNLSYAGHDL

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260898743, Length=344, Percent_Identity=22.9651162790698, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI4758786, Length=344, Percent_Identity=22.9651162790698, Blast_Score=76, Evalue=9e-14,
Organism=Escherichia coli, GI1789076, Length=495, Percent_Identity=27.8787878787879, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1788832, Length=438, Percent_Identity=28.310502283105, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI145693162, Length=380, Percent_Identity=22.3684210526316, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17568379, Length=255, Percent_Identity=23.1372549019608, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17555284, Length=257, Percent_Identity=22.568093385214, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI221459469, Length=276, Percent_Identity=26.4492753623188, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24638644, Length=335, Percent_Identity=23.8805970149254, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 55801; Mature: 55669

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPDLGVELERLVAPLPMWHGRLGQDAWQAIACVVANAGGRLVAVWGAERDPDAMATAC
CCCCCCCCCHHHHHCCCCHHCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHH
VAYAMPHGLVWLELQLDGARAIPDLAGVFPCASRMQRAMTDLSGVSVEGAHDTRPWLDHG
HHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHCC
LWPPGARPLQSSGPGFAVAAGMLPADYAFLRVEGDGVHEVAVGPVHAGIIEPGHFRFSVV
CCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCEEEEECCEEECCCCCCCEEEEHH
GEKVLRLEERLGYTHKGIERRFTELAPEEGYRLAGRVSGDSTVAYAWAYCMALESACRMT
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCC
LPARAACLRALMLERERVANHLGDLGALGNDAGLAFGLAQFSRLREDWQRLSGQVFGHRL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MMDVVVPGGVAIDTSAPALERLGRQCDEIERELHVLRAIYDEHAGLQDRFRGTGQITPQL
EEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHH
AARLGLTGLAGRASGQAVDLRCDVAWLSYETLAVRMATQNVGDVAARVAVRFDEVIESLR
HHHHCCCCCCCCCCCCEEEEEEEEEEEEHHHHEEEHHHCCHHHHHHHHHHHHHHHHHHHH
LIRALCTGLPEGEIRGELRLPSVRAIGAGWVEGWRGEVLVALELGADDRVLRCHCHDPSW
HHHHHHCCCCCCCCCCCEECCCHHCCCCHHHCCCCCCEEEEEEECCCCCEEEEEECCCCC
QNWPALEHAIIGNIVPDFPLINKSFNLSYAGHDL
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQPDLGVELERLVAPLPMWHGRLGQDAWQAIACVVANAGGRLVAVWGAERDPDAMATAC
CCCCCCCCHHHHHCCCCHHCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHH
VAYAMPHGLVWLELQLDGARAIPDLAGVFPCASRMQRAMTDLSGVSVEGAHDTRPWLDHG
HHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHCC
LWPPGARPLQSSGPGFAVAAGMLPADYAFLRVEGDGVHEVAVGPVHAGIIEPGHFRFSVV
CCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCEEEEECCEEECCCCCCCEEEEHH
GEKVLRLEERLGYTHKGIERRFTELAPEEGYRLAGRVSGDSTVAYAWAYCMALESACRMT
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCC
LPARAACLRALMLERERVANHLGDLGALGNDAGLAFGLAQFSRLREDWQRLSGQVFGHRL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MMDVVVPGGVAIDTSAPALERLGRQCDEIERELHVLRAIYDEHAGLQDRFRGTGQITPQL
EEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHH
AARLGLTGLAGRASGQAVDLRCDVAWLSYETLAVRMATQNVGDVAARVAVRFDEVIESLR
HHHHCCCCCCCCCCCCEEEEEEEEEEEEHHHHEEEHHHCCHHHHHHHHHHHHHHHHHHHH
LIRALCTGLPEGEIRGELRLPSVRAIGAGWVEGWRGEVLVALELGADDRVLRCHCHDPSW
HHHHHHCCCCCCCCCCCEECCCHHCCCCHHHCCCCCCEEEEEEECCCCCEEEEEECCCCC
QNWPALEHAIIGNIVPDFPLINKSFNLSYAGHDL
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503; 8125094 [H]