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The map label for this gene is hyfI [H]

Identifier: 94313588

GI number: 94313588

Start: 1298006

End: 1298521

Strand: Reverse

Name: hyfI [H]

Synonym: Rmet_4666

Alternate gene names: 94313588

Gene position: 1298521-1298006 (Counterclockwise)

Preceding gene: 94313589

Following gene: 94313586

Centisome position: 50.33

GC content: 63.18

Gene sequence:

>516_bases
ATGTGGCAAATACTGAAGCAGATCGCGCGTACCGGCATTCTCACGGAGCCTGCGCCGCAAGGTAGTGCCGACGACACCGC
GATCGAGCGTATCCACAAGGACCTGCTCGATATCCTCGGCCGCGCACTGACCATCCGGCAGGTCGACGCCGGCTCCTGCA
ACGGCTGTGAGCTTGAGATCCACGCGCTCGGCAACCCCTACTACAACATCGAAGGACTGGGCGTCAGGTTCGTGGCTAGC
CCGCGGCATGCCGACATGCTGCTCGTCACCGGGCCGGTCTCACGCCATATGGAGGAAGCATTGCGGCGTACCTACGAGGC
AACGCCCGAGCCAAAACTGGTGGTCGCCGTGGGAGATTGCGGGTGCACCAGTGGCATCTTCGGCGAAAGCTACGCGAGCT
GCGGCCGTGTCTCCAATGTCATTCCAGTCGATGTGAGCGTCCCGGGTTGCCCACCGCCACCCATCGACATCCTGCGAGGC
ATCCTTGGCGCCGTTCTGCGCGATCGCGTTGGTTAG

Upstream 100 bases:

>100_bases
ACTGGCCGGCGCTCGAGCATGCCATTATCGGCAACATCGTTCCGGACTTTCCGCTGATCAACAAGTCCTTCAACCTCAGC
TATGCGGGGCACGACCTCTG

Downstream 100 bases:

>100_bases
CGGGTACAAATTGTCCTGTTGCGTCAGGACGGGTCCCCGACCTTCGGGCGGGTCGCATAGTCAACGCCGTGTCGATTCAG
GTACTCGCGGATGAGTTGGC

Product: Hydrogenase-4 subunit I

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MWQILKQIARTGILTEPAPQGSADDTAIERIHKDLLDILGRALTIRQVDAGSCNGCELEIHALGNPYYNIEGLGVRFVAS
PRHADMLLVTGPVSRHMEEALRRTYEATPEPKLVVAVGDCGCTSGIFGESYASCGRVSNVIPVDVSVPGCPPPPIDILRG
ILGAVLRDRVG

Sequences:

>Translated_171_residues
MWQILKQIARTGILTEPAPQGSADDTAIERIHKDLLDILGRALTIRQVDAGSCNGCELEIHALGNPYYNIEGLGVRFVAS
PRHADMLLVTGPVSRHMEEALRRTYEATPEPKLVVAVGDCGCTSGIFGESYASCGRVSNVIPVDVSVPGCPPPPIDILRG
ILGAVLRDRVG
>Mature_171_residues
MWQILKQIARTGILTEPAPQGSADDTAIERIHKDLLDILGRALTIRQVDAGSCNGCELEIHALGNPYYNIEGLGVRFVAS
PRHADMLLVTGPVSRHMEEALRRTYEATPEPKLVVAVGDCGCTSGIFGESYASCGRVSNVIPVDVSVPGCPPPPIDILRG
ILGAVLRDRVG

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=145, Percent_Identity=33.1034482758621, Blast_Score=93, Evalue=1e-19,
Organism=Escherichia coli, GI1789074, Length=141, Percent_Identity=41.1347517730496, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1788834, Length=153, Percent_Identity=38.562091503268, Blast_Score=123, Evalue=5e-30,
Organism=Escherichia coli, GI1788624, Length=166, Percent_Identity=28.3132530120482, Blast_Score=73, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17509685, Length=158, Percent_Identity=36.0759493670886, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI18859983, Length=161, Percent_Identity=32.9192546583851, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24642371, Length=161, Percent_Identity=32.9192546583851, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24651058, Length=130, Percent_Identity=36.9230769230769, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 18297; Mature: 18297

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00131 CARBOXYPEPT_SER_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWQILKQIARTGILTEPAPQGSADDTAIERIHKDLLDILGRALTIRQVDAGSCNGCELEI
CHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEE
HALGNPYYNIEGLGVRFVASPRHADMLLVTGPVSRHMEEALRRTYEATPEPKLVVAVGDC
EECCCCEEEECCCCEEEEECCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEECCC
GCTSGIFGESYASCGRVSNVIPVDVSVPGCPPPPIDILRGILGAVLRDRVG
CCCCCCCCCHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MWQILKQIARTGILTEPAPQGSADDTAIERIHKDLLDILGRALTIRQVDAGSCNGCELEI
CHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEE
HALGNPYYNIEGLGVRFVASPRHADMLLVTGPVSRHMEEALRRTYEATPEPKLVVAVGDC
EECCCCEEEECCCCEEEEECCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEECCC
GCTSGIFGESYASCGRVSNVIPVDVSVPGCPPPPIDILRGILGAVLRDRVG
CCCCCCCCCHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]