Click here to switch to the map view.

The map label for this gene is rutR [H]

Identifier: 94313491

GI number: 94313491

Start: 1182539

End: 1183204

Strand: Reverse

Name: rutR [H]

Synonym: Rmet_4569

Alternate gene names: 94313491

Gene position: 1183204-1182539 (Counterclockwise)

Preceding gene: 94313492

Following gene: 94313490

Centisome position: 45.86

GC content: 67.27

Gene sequence:

>666_bases
ATGATGGGCGCGCCGGCGCTTGCGCCGCCCGTGCGCGAATCGGGAGGCCGTATCCGGCAGGAGAATGAGGCGCAGATCCT
GCGCGCCGCGGAACACGTGTTCGCGCGCGCGGGGTTCGCCGGGGCCACGATGGCCGACATCGCGCTGCACGCCGGCATGC
CGAAGTCGAACCTGCACTACTACTTCCGGACGAAGCAGGCGCTGTACCGCGCCGTGCTGGCGCACACGTTGCAGCTCTGG
CTATCGGAGACCGATATCATCAGCGCCGACCATGCGCCGCGCGTGGCGCTGGAGCAGTACATCCGCGCCAAGATGCGGCT
GTCGGCCAGCCATCCGGACGCGTCTCGCGTGTTCGCCAACGAGTTGCTGCACGGCGCCCCTGAGATCGGCGGCGTGCTGC
GTGAAGCGTTGCGCGAACTGGTGACGCGCAAGGCCATGGTGATTCGCTCATGGATTGAAGACGGTCAGATGGCCCGTGTC
GACCCCTATCACCTCTTCTTCACGATCTGGGCCGCCACGCAGACGTACGCGGACTTCGATGCGCAGGTCTGCGCCGTGCT
GGGCGTCAGCCAGCTCGACACCGAGGACTTCGAACGCGCCACCGAGCACCTGGTGGCATTGCTGCTGCGTGGCTGCGGGC
TGGATCAGGAAGCCTCGCTGGCCTGA

Upstream 100 bases:

>100_bases
ATGTCTGCATCGTCACGGCGGCCATTGACGAGCAACTCAACGAGCACGGCTATATCGTGCCCGGTCTCGGCGATGCCGGT
GACCGGCTCTACGGCACCAA

Downstream 100 bases:

>100_bases
GCCAGCATCTAGCATGGCAGTTGCCATGCAGTCTTCGTCTACCATTGCCACGTCACCGCCACCGGGATCACAAGGGACGC
CTCATGTTCGACACCATCCT

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: Rut operon repressor [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MMGAPALAPPVRESGGRIRQENEAQILRAAEHVFARAGFAGATMADIALHAGMPKSNLHYYFRTKQALYRAVLAHTLQLW
LSETDIISADHAPRVALEQYIRAKMRLSASHPDASRVFANELLHGAPEIGGVLREALRELVTRKAMVIRSWIEDGQMARV
DPYHLFFTIWAATQTYADFDAQVCAVLGVSQLDTEDFERATEHLVALLLRGCGLDQEASLA

Sequences:

>Translated_221_residues
MMGAPALAPPVRESGGRIRQENEAQILRAAEHVFARAGFAGATMADIALHAGMPKSNLHYYFRTKQALYRAVLAHTLQLW
LSETDIISADHAPRVALEQYIRAKMRLSASHPDASRVFANELLHGAPEIGGVLREALRELVTRKAMVIRSWIEDGQMARV
DPYHLFFTIWAATQTYADFDAQVCAVLGVSQLDTEDFERATEHLVALLLRGCGLDQEASLA
>Mature_221_residues
MMGAPALAPPVRESGGRIRQENEAQILRAAEHVFARAGFAGATMADIALHAGMPKSNLHYYFRTKQALYRAVLAHTLQLW
LSETDIISADHAPRVALEQYIRAKMRLSASHPDASRVFANELLHGAPEIGGVLREALRELVTRKAMVIRSWIEDGQMARV
DPYHLFFTIWAATQTYADFDAQVCAVLGVSQLDTEDFERATEHLVALLLRGCGLDQEASLA

Specific function: Master transcription regulator which represses the degradation of pyrimidines (rutABCDEFG) and purines (gcl operon) for maintenance of metabolic balance between pyrimidines and purines. It also regulates the synthesis of pyrimidine nucleotides and arginin

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787249, Length=204, Percent_Identity=40.1960784313725, Blast_Score=154, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR019915
- InterPro:   IPR001647
- InterPro:   IPR013573 [H]

Pfam domain/function: PF08362 TetR_C_3; PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 24451; Mature: 24451

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMGAPALAPPVRESGGRIRQENEAQILRAAEHVFARAGFAGATMADIALHAGMPKSNLHY
CCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCE
YFRTKQALYRAVLAHTLQLWLSETDIISADHAPRVALEQYIRAKMRLSASHPDASRVFAN
EHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
ELLHGAPEIGGVLREALRELVTRKAMVIRSWIEDGQMARVDPYHLFFTIWAATQTYADFD
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHH
AQVCAVLGVSQLDTEDFERATEHLVALLLRGCGLDQEASLA
HHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MMGAPALAPPVRESGGRIRQENEAQILRAAEHVFARAGFAGATMADIALHAGMPKSNLHY
CCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCE
YFRTKQALYRAVLAHTLQLWLSETDIISADHAPRVALEQYIRAKMRLSASHPDASRVFAN
EHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
ELLHGAPEIGGVLREALRELVTRKAMVIRSWIEDGQMARVDPYHLFFTIWAATQTYADFD
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHH
AQVCAVLGVSQLDTEDFERATEHLVALLLRGCGLDQEASLA
HHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]