BacMap

Definition	Cupriavidus metallidurans CH34 megaplasmid, complete sequence.
Accession	NC_007974
Length	2,580,084

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The map label for this gene is fabG [C]

Identifier: 94313339

GI number: 94313339

Start: 1010046

End: 1010960

Strand: Reverse

Name: fabG [C]

Synonym: Rmet_4414

Alternate gene names: 94313339

Gene position: 1010960-1010046 (Counterclockwise)

Preceding gene: 94313340

Following gene: 94313338

Centisome position: 39.18

GC content: 65.46

Gene sequence:

>915_bases
ATGCAAGGAATCATGGAAGGCAAGGTAGCGCTGGTCACGGGCGCGGGTGGTGGTATCGGACGCGGTATCGCGCTGGCCCT
CGGGGCCGCTGGCGCCCGGGTAGTCGTCAACGACCTCGGGGTGTCGATGAGCGGCGAAGGCGGCGATGCCGGCCCCGCGC
AGCGCGTGGCCGAGGAAATCCGCGCGGCCGGCGGCCAGGCGGTGGCGAATACTGACAGCGTGTCTACGTGGAACGGCGCT
CACAACATCATCCAGTGCGCGCTAGACAACTTCGGCCGCATCGACGCCGTGGTCAACAACGCTGGCAACCTGCGTGACCG
CATGTTCTTCAAAATGAACGAGGAAGAATGGCGCTCGGTGATCGACGTGCATCTGCACGGCACGTTCTTCGTCAGCCGCG
CTGCCGCCAACCATTTCAAGGACCAGGAAGGCGGCGCGTATGTGCACATGACGTCGACCTCCGGCCTGATCGGCAACTTT
GGCCAGGCCAACTACGCCGCGGCCAAGCTCGGCATCGTCGCACTGTCCAAGTCGATCGCGCTGGACATGCAACGCTTCAA
CGTGCGCTCGAACTGCATCGCGCCGTTTGCCTGGAGCCGCATGACCAGCTCGATCCCGGCCGAGACGCCCGAGGAGAAAG
CCCGCGTGGCCAAGCTGCAAAGGATGGAGGCCGGCAAGATCGCGCCGATGGCCGTATTCCTGGCGAGCGACGCGGCGCGC
GATGTCAGCGGCCAGATCTTCGCCGTGCGCGCCAACGAGATCATGCTGATGAGCCATTCGCGCCCGATCCGCTCGGTGCA
CATGGGCGAAGGCTGGACACCCGAGGCCATCGGCGAAGTCGCCGTGCCGGCCATGCGCAACAGCTTCTACAAGCTCGAAC
GCTCCGCCGACGTGATCGGCTGGGACCCGATCTGA

Upstream 100 bases:

>100_bases
ACCTGACGCTTTGGAGCGACGTGATCAGGAAAGCCAACATCGAGCCGCAATAAGCCTGCCGACTCGGCACCCAATCTCAC
CAACATTCAGGAGCAACACG

Downstream 100 bases:

>100_bases
GGGGCTGCGCATGAGCATCAAGGGCAAGGCCTATATCGTCGGGGCCTACGAGCACCCGACGCGCAAGGCGCCGGACAAAT
CCGTCGCGCAGCTTCACGCC

Product: 3-oxoacyl-(acyl-carrier-protein) reductase

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MQGIMEGKVALVTGAGGGIGRGIALALGAAGARVVVNDLGVSMSGEGGDAGPAQRVAEEIRAAGGQAVANTDSVSTWNGA
HNIIQCALDNFGRIDAVVNNAGNLRDRMFFKMNEEEWRSVIDVHLHGTFFVSRAAANHFKDQEGGAYVHMTSTSGLIGNF
GQANYAAAKLGIVALSKSIALDMQRFNVRSNCIAPFAWSRMTSSIPAETPEEKARVAKLQRMEAGKIAPMAVFLASDAAR
DVSGQIFAVRANEIMLMSHSRPIRSVHMGEGWTPEAIGEVAVPAMRNSFYKLERSADVIGWDPI

Sequences:

>Translated_304_residues
MQGIMEGKVALVTGAGGGIGRGIALALGAAGARVVVNDLGVSMSGEGGDAGPAQRVAEEIRAAGGQAVANTDSVSTWNGA
HNIIQCALDNFGRIDAVVNNAGNLRDRMFFKMNEEEWRSVIDVHLHGTFFVSRAAANHFKDQEGGAYVHMTSTSGLIGNF
GQANYAAAKLGIVALSKSIALDMQRFNVRSNCIAPFAWSRMTSSIPAETPEEKARVAKLQRMEAGKIAPMAVFLASDAAR
DVSGQIFAVRANEIMLMSHSRPIRSVHMGEGWTPEAIGEVAVPAMRNSFYKLERSADVIGWDPI
>Mature_304_residues
MQGIMEGKVALVTGAGGGIGRGIALALGAAGARVVVNDLGVSMSGEGGDAGPAQRVAEEIRAAGGQAVANTDSVSTWNGA
HNIIQCALDNFGRIDAVVNNAGNLRDRMFFKMNEEEWRSVIDVHLHGTFFVSRAAANHFKDQEGGAYVHMTSTSGLIGNF
GQANYAAAKLGIVALSKSIALDMQRFNVRSNCIAPFAWSRMTSSIPAETPEEKARVAKLQRMEAGKIAPMAVFLASDAAR
DVSGQIFAVRANEIMLMSHSRPIRSVHMGEGWTPEAIGEVAVPAMRNSFYKLERSADVIGWDPI

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4504505, Length=275, Percent_Identity=43.2727272727273, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI15277342, Length=256, Percent_Identity=30.46875, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI40254992, Length=243, Percent_Identity=27.9835390946502, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI19923817, Length=307, Percent_Identity=25.0814332247557, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI59889578, Length=221, Percent_Identity=28.9592760180996, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI31542939, Length=210, Percent_Identity=31.4285714285714, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI32483357, Length=259, Percent_Identity=25.8687258687259, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI4758504, Length=217, Percent_Identity=30.4147465437788, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI224922801, Length=185, Percent_Identity=30.8108108108108, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI10190704, Length=255, Percent_Identity=27.0588235294118, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI83715985, Length=193, Percent_Identity=31.0880829015544, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI209862901, Length=268, Percent_Identity=26.4925373134328, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI19923983, Length=268, Percent_Identity=26.4925373134328, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4503817, Length=228, Percent_Identity=23.2456140350877, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787335, Length=259, Percent_Identity=34.7490347490347, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1789208, Length=254, Percent_Identity=31.1023622047244, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1787905, Length=248, Percent_Identity=31.8548387096774, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI87082100, Length=253, Percent_Identity=30.0395256916996, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790717, Length=253, Percent_Identity=30.0395256916996, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082160, Length=251, Percent_Identity=27.0916334661355, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1788459, Length=247, Percent_Identity=29.9595141700405, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787526, Length=253, Percent_Identity=25.6916996047431, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786812, Length=264, Percent_Identity=27.6515151515151, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI2367175, Length=244, Percent_Identity=29.0983606557377, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789057, Length=273, Percent_Identity=26.007326007326, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17568967, Length=243, Percent_Identity=41.5637860082305, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI25147288, Length=253, Percent_Identity=34.7826086956522, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17555706, Length=250, Percent_Identity=30.8, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17560676, Length=251, Percent_Identity=30.2788844621514, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17564282, Length=221, Percent_Identity=27.1493212669683, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17538182, Length=220, Percent_Identity=32.7272727272727, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71997099, Length=201, Percent_Identity=30.8457711442786, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17561402, Length=254, Percent_Identity=25.1968503937008, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI193203117, Length=193, Percent_Identity=25.9067357512953, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI193203115, Length=193, Percent_Identity=25.9067357512953, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17531453, Length=199, Percent_Identity=27.6381909547739, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17544670, Length=197, Percent_Identity=25.8883248730964, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17562906, Length=201, Percent_Identity=26.3681592039801, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI72000259, Length=198, Percent_Identity=29.2929292929293, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71994604, Length=254, Percent_Identity=25.9842519685039, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17555612, Length=201, Percent_Identity=29.3532338308458, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17559104, Length=197, Percent_Identity=27.4111675126904, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71994600, Length=257, Percent_Identity=24.9027237354086, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17562908, Length=197, Percent_Identity=24.8730964467005, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI193204405, Length=199, Percent_Identity=27.1356783919598, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=276, Percent_Identity=41.6666666666667, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6324126, Length=251, Percent_Identity=30.2788844621514, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI18859837, Length=236, Percent_Identity=43.6440677966102, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24639444, Length=249, Percent_Identity=32.5301204819277, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17737361, Length=225, Percent_Identity=30.6666666666667, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI23397609, Length=256, Percent_Identity=26.5625, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281364892, Length=216, Percent_Identity=25.462962962963, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24649181, Length=182, Percent_Identity=28.021978021978, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21355319, Length=248, Percent_Identity=24.5967741935484, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24644339, Length=190, Percent_Identity=24.7368421052632, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 32288; Mature: 32288

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGIMEGKVALVTGAGGGIGRGIALALGAAGARVVVNDLGVSMSGEGGDAGPAQRVAEEI
CCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCEEECCCCCCCCHHHHHHHHH
RAAGGQAVANTDSVSTWNGAHNIIQCALDNFGRIDAVVNNAGNLRDRMFFKMNEEEWRSV
HHCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCCCHHEEEEECHHHHHHE
IDVHLHGTFFVSRAAANHFKDQEGGAYVHMTSTSGLIGNFGQANYAAAKLGIVALSKSIA
EEEEECCCEEEHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LDMQRFNVRSNCIAPFAWSRMTSSIPAETPEEKARVAKLQRMEAGKIAPMAVFLASDAAR
HHHHHHCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
DVSGQIFAVRANEIMLMSHSRPIRSVHMGEGWTPEAIGEVAVPAMRNSFYKLERSADVIG
CCCCCEEEEECCEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
WDPI
CCCC
>Mature Secondary Structure
MQGIMEGKVALVTGAGGGIGRGIALALGAAGARVVVNDLGVSMSGEGGDAGPAQRVAEEI
CCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCEEECCCCCCCCHHHHHHHHH
RAAGGQAVANTDSVSTWNGAHNIIQCALDNFGRIDAVVNNAGNLRDRMFFKMNEEEWRSV
HHCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCCCHHEEEEECHHHHHHE
IDVHLHGTFFVSRAAANHFKDQEGGAYVHMTSTSGLIGNFGQANYAAAKLGIVALSKSIA
EEEEECCCEEEHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LDMQRFNVRSNCIAPFAWSRMTSSIPAETPEEKARVAKLQRMEAGKIAPMAVFLASDAAR
HHHHHHCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
DVSGQIFAVRANEIMLMSHSRPIRSVHMGEGWTPEAIGEVAVPAMRNSFYKLERSADVIG
CCCCCEEEEECCEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
WDPI
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]