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The map label for this gene is potF [H]

Identifier: 94313321

GI number: 94313321

Start: 992677

End: 993765

Strand: Reverse

Name: potF [H]

Synonym: Rmet_4396

Alternate gene names: 94313321

Gene position: 993765-992677 (Counterclockwise)

Preceding gene: 94313322

Following gene: 94313319

Centisome position: 38.52

GC content: 63.54

Gene sequence:

>1089_bases
ATGAGTCATCGCATATGGACGAAGGTGCGCCATCTGCTGGTGAGCCTGACGCTGATGGGGTGCTTTGGCCTGGCCATGGC
CACGGAAGACCTGAACGTATTCACCTGGAACAACGCGCTGTCGCCCGAGACGATTGCCCGATTCGAGTCCGAATGCGCCT
GCCACGTGAACCTGACGTATTTCGGTTCGATGGAGGAAGCGCTGGCCAAGCTTGCGGGCGGCGCGCAAGGGTTCGACGTG
ATATGTCCGAGCAACTACGGCATTCCGGCGCTGATCCGCCTGGGGCTGCTCCGGCCGCTGGACAAGCACGCCATCCCGAA
TCTTCGCAATATCGACCCGGCGTTCCTGAACACGCCGCTTGACCCGGGCAACGTCTATTCCCTGCCCTACGATTTCACGA
TCACGCTGGTCGGCTACAACGCCACGCGCCTGCACGATCTGGGGCTCGATGCGTCGTCATGGTCGCTGGTTTTCGACCCG
CACATGCTGGAGAAGCTGCGGGGCCGTGTCACCGTGCTGGACGACCCCCGCGAAGTCATCGCGGCTGCGTTGCGCTACAA
GGGCCACTCCGGTAATTCGACGGACCCTGCGCAACTGCGCGAAGCCGAGGCGGCCATCCGCGCCGCGCTGCCCTACTGGG
CGGCGTTCAACAGCCAGAGCTATATCCGCGAGCTTACGGTCGGCAATATCTGGGTGGTGCTCGGCTATTCGAGCGATGTC
TATCAGGCCGGGTTGGACGCGCGCAACACCCACCGGGCTTTCTCGATCACCTCGTCACTGCAGCGCGAGGGCAATGGCAT
GACCGCCGACAGTTTCGTGGTCACAAAGACGGCGCCGCATCCCGCGCTGGCGTACAGGTTCATCAACTTCATGCTGCGCC
CTGAAAATGCCGCTGAGATCACTAATGCCATCGGCGCGGGCAATCCGAATGCCGCGGCGCGCGCGTTCATCCGGCCAGAC
CTGCTGGCCGACCCGGTCATCACGCCGGGTGTCACGCAGCGACAGCGGCTCGAACAGTTGAACGACCTCGACAGCCGCAC
ACGGCGGGCGTGGAATCGAGCGTGGACCGACCTCAAGGTCGGCCACTAG

Upstream 100 bases:

>100_bases
CGCCGGAAGTGAATGCGATCTCGTCCATCCTCATGATGCTGACACTGGCCGTGTCGCTCACGGCCTATCGCGTTTCGCGC
GACGTCTGGAGGGCGCAACT

Downstream 100 bases:

>100_bases
CGATTCGATCGGGAAAGGATTGGGATGGGGAATTACGCGGAAGTGGTGCTTTGGCCGGCGGCCACTTCCTCGGCCAGCGG
AATGTCCCCACGCACCACCA

Product: putrescine ABC transporter, periplasmic putrescine-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MSHRIWTKVRHLLVSLTLMGCFGLAMATEDLNVFTWNNALSPETIARFESECACHVNLTYFGSMEEALAKLAGGAQGFDV
ICPSNYGIPALIRLGLLRPLDKHAIPNLRNIDPAFLNTPLDPGNVYSLPYDFTITLVGYNATRLHDLGLDASSWSLVFDP
HMLEKLRGRVTVLDDPREVIAAALRYKGHSGNSTDPAQLREAEAAIRAALPYWAAFNSQSYIRELTVGNIWVVLGYSSDV
YQAGLDARNTHRAFSITSSLQREGNGMTADSFVVTKTAPHPALAYRFINFMLRPENAAEITNAIGAGNPNAAARAFIRPD
LLADPVITPGVTQRQRLEQLNDLDSRTRRAWNRAWTDLKVGH

Sequences:

>Translated_362_residues
MSHRIWTKVRHLLVSLTLMGCFGLAMATEDLNVFTWNNALSPETIARFESECACHVNLTYFGSMEEALAKLAGGAQGFDV
ICPSNYGIPALIRLGLLRPLDKHAIPNLRNIDPAFLNTPLDPGNVYSLPYDFTITLVGYNATRLHDLGLDASSWSLVFDP
HMLEKLRGRVTVLDDPREVIAAALRYKGHSGNSTDPAQLREAEAAIRAALPYWAAFNSQSYIRELTVGNIWVVLGYSSDV
YQAGLDARNTHRAFSITSSLQREGNGMTADSFVVTKTAPHPALAYRFINFMLRPENAAEITNAIGAGNPNAAARAFIRPD
LLADPVITPGVTQRQRLEQLNDLDSRTRRAWNRAWTDLKVGH
>Mature_361_residues
SHRIWTKVRHLLVSLTLMGCFGLAMATEDLNVFTWNNALSPETIARFESECACHVNLTYFGSMEEALAKLAGGAQGFDVI
CPSNYGIPALIRLGLLRPLDKHAIPNLRNIDPAFLNTPLDPGNVYSLPYDFTITLVGYNATRLHDLGLDASSWSLVFDPH
MLEKLRGRVTVLDDPREVIAAALRYKGHSGNSTDPAQLREAEAAIRAALPYWAAFNSQSYIRELTVGNIWVVLGYSSDVY
QAGLDARNTHRAFSITSSLQREGNGMTADSFVVTKTAPHPALAYRFINFMLRPENAAEITNAIGAGNPNAAARAFIRPDL
LADPVITPGVTQRQRLEQLNDLDSRTRRAWNRAWTDLKVGH

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=346, Percent_Identity=32.9479768786127, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1787367, Length=319, Percent_Identity=30.7210031347962, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1787711, Length=277, Percent_Identity=27.0758122743682, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40005; Mature: 39874

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00678 WD_REPEATS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHRIWTKVRHLLVSLTLMGCFGLAMATEDLNVFTWNNALSPETIARFESECACHVNLTY
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCEEEEEEEE
FGSMEEALAKLAGGAQGFDVICPSNYGIPALIRLGLLRPLDKHAIPNLRNIDPAFLNTPL
ECCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCHHHHCCCC
DPGNVYSLPYDFTITLVGYNATRLHDLGLDASSWSLVFDPHMLEKLRGRVTVLDDPREVI
CCCCEEECCCCEEEEEEECCCCEEEECCCCCCCCEEEECHHHHHHHCCCEEEECCHHHHH
AAALRYKGHSGNSTDPAQLREAEAAIRAALPYWAAFNSQSYIRELTVGNIWVVLGYSSDV
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEECCCHH
YQAGLDARNTHRAFSITSSLQREGNGMTADSFVVTKTAPHPALAYRFINFMLRPENAAEI
HHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHH
TNAIGAGNPNAAARAFIRPDLLADPVITPGVTQRQRLEQLNDLDSRTRRAWNRAWTDLKV
HHHHCCCCCCHHHHHHCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEC
GH
CC
>Mature Secondary Structure 
SHRIWTKVRHLLVSLTLMGCFGLAMATEDLNVFTWNNALSPETIARFESECACHVNLTY
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCEEEEEEEE
FGSMEEALAKLAGGAQGFDVICPSNYGIPALIRLGLLRPLDKHAIPNLRNIDPAFLNTPL
ECCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCHHHHCCCC
DPGNVYSLPYDFTITLVGYNATRLHDLGLDASSWSLVFDPHMLEKLRGRVTVLDDPREVI
CCCCEEECCCCEEEEEEECCCCEEEECCCCCCCCEEEECHHHHHHHCCCEEEECCHHHHH
AAALRYKGHSGNSTDPAQLREAEAAIRAALPYWAAFNSQSYIRELTVGNIWVVLGYSSDV
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEECCCHH
YQAGLDARNTHRAFSITSSLQREGNGMTADSFVVTKTAPHPALAYRFINFMLRPENAAEI
HHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHH
TNAIGAGNPNAAARAFIRPDLLADPVITPGVTQRQRLEQLNDLDSRTRRAWNRAWTDLKV
HHHHCCCCCCHHHHHHCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEC
GH
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]