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The map label for this gene is qor [C]

Identifier: 94313295

GI number: 94313295

Start: 968479

End: 969495

Strand: Direct

Name: qor [C]

Synonym: Rmet_4370

Alternate gene names: 94313295

Gene position: 968479-969495 (Clockwise)

Preceding gene: 94313294

Following gene: 94313298

Centisome position: 37.54

GC content: 62.73

Gene sequence:

>1017_bases
ATGAAAGCCATTGGTCTGACCCGTTACCTGCCCATCAGCGATCCGCAGTCGCTCGTCGATGTCGAGATCGCCCAGCCCGA
GGCCAGCGGCCATGACCTGCTGGTCAAGGTGGAGGCAATCTCGGTGAATCCCGTGGACACCAAGGTCCGGGCGCCGAAAG
ACAAGGTCGAGGATGCGCCGCGCGTGTTGGGCTGGGATGCCGCTGGCACCGTCGTGGCTGTCGGCGCGGATGTCACGCTG
TTCAAGGTAGGCGATCCCGTGTTCTACGCCGGCAGCATTACGCGCCCGGGCGCGAATAGCGAGTACCACCTGGTAGACGA
ACGGATCGTCGGCCATAAGCCGGCTTCGCTCGATTTCCCGCAAGCGGCAGCCTTGCCGCTGACAGCCATCACGGCCTGGG
AAGCGATGTTCGATCGCCTGCGTATCTCGCCGAAAGGCGAGGACGCCGGAAAATCGGTGTTGATTATTGGTGGCGCGGGT
GGGGTAGGGTCGATCGGCATCCAGCTTGCCAAGGTTCTGGCTGGCTTGACGGTGATTGCCACGGCGTCCCGGCCGGAATC
TGAAGCCTGGTGCCGCGGGCTTGGGGCCGACCATACGATTGATCATCACGGCGACATCCCGGCCCAACTGCGCGCGCTGG
GTTTCGCGCATGTGGACTTCGTCCTCTGCTTCAACGACACCGATCGCCATTTCAACGCCATGGCCGAGGCTATCGCCCCG
CAGGGAACGATCTGCACGATCGTGGAGAATACCGGCCCCCTGGCCATTTCGGCGCTCAAGAACAAGAGCGCCGGCTTTGT
CTGGGAATTAATGTTCACGCGGGCGATGTTCCAGACGCCGGACATAATTGCGCAACACCGGCTGCTGAATGAGGTGGCGC
GGTTGATCGATGCCGGCAAGCTGCGGACTACGGTGGGTGACAACCTCGGCACCATCAATGCGGCCAATCTGCGTCGCGCC
CATGCGCTGCTCGAAGGCGGGCGAACGATCGGCAAACTTGTGCTCTCCGGCTTCTGA

Upstream 100 bases:

>100_bases
TTTCCCGATATGCGGGAAAGTGATTGCGGGATCTTCCGGCTAATCGTCGGGATCGTTTGCGACTAGCATCCGCTTATCTC
TCCCTTCAAAGGATGCCGCC

Downstream 100 bases:

>100_bases
TAGCGGTATATCTGCAATTAACTGCCAGGCAGCGCGCGACACCAATATGAAGGGGCGGGCCTCTCGCGAGGCCCGCCCCT
TTTGCTTTGCTTGCAGTCGG

Product: alcohol dehydrogenase Zinc-containing GroES-like protein

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKAIGLTRYLPISDPQSLVDVEIAQPEASGHDLLVKVEAISVNPVDTKVRAPKDKVEDAPRVLGWDAAGTVVAVGADVTL
FKVGDPVFYAGSITRPGANSEYHLVDERIVGHKPASLDFPQAAALPLTAITAWEAMFDRLRISPKGEDAGKSVLIIGGAG
GVGSIGIQLAKVLAGLTVIATASRPESEAWCRGLGADHTIDHHGDIPAQLRALGFAHVDFVLCFNDTDRHFNAMAEAIAP
QGTICTIVENTGPLAISALKNKSAGFVWELMFTRAMFQTPDIIAQHRLLNEVARLIDAGKLRTTVGDNLGTINAANLRRA
HALLEGGRTIGKLVLSGF

Sequences:

>Translated_338_residues
MKAIGLTRYLPISDPQSLVDVEIAQPEASGHDLLVKVEAISVNPVDTKVRAPKDKVEDAPRVLGWDAAGTVVAVGADVTL
FKVGDPVFYAGSITRPGANSEYHLVDERIVGHKPASLDFPQAAALPLTAITAWEAMFDRLRISPKGEDAGKSVLIIGGAG
GVGSIGIQLAKVLAGLTVIATASRPESEAWCRGLGADHTIDHHGDIPAQLRALGFAHVDFVLCFNDTDRHFNAMAEAIAP
QGTICTIVENTGPLAISALKNKSAGFVWELMFTRAMFQTPDIIAQHRLLNEVARLIDAGKLRTTVGDNLGTINAANLRRA
HALLEGGRTIGKLVLSGF
>Mature_338_residues
MKAIGLTRYLPISDPQSLVDVEIAQPEASGHDLLVKVEAISVNPVDTKVRAPKDKVEDAPRVLGWDAAGTVVAVGADVTL
FKVGDPVFYAGSITRPGANSEYHLVDERIVGHKPASLDFPQAAALPLTAITAWEAMFDRLRISPKGEDAGKSVLIIGGAG
GVGSIGIQLAKVLAGLTVIATASRPESEAWCRGLGADHTIDHHGDIPAQLRALGFAHVDFVLCFNDTDRHFNAMAEAIAP
QGTICTIVENTGPLAISALKNKSAGFVWELMFTRAMFQTPDIIAQHRLLNEVARLIDAGKLRTTVGDNLGTINAANLRRA
HALLEGGRTIGKLVLSGF

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI47519420, Length=341, Percent_Identity=32.258064516129, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI194239674, Length=204, Percent_Identity=33.8235294117647, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI13236495, Length=204, Percent_Identity=33.8235294117647, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI194239676, Length=204, Percent_Identity=33.8235294117647, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI24308257, Length=303, Percent_Identity=25.7425742574257, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI18379349, Length=220, Percent_Identity=31.3636363636364, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI22538446, Length=336, Percent_Identity=26.1904761904762, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI22538444, Length=336, Percent_Identity=26.1904761904762, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1790485, Length=330, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71987554, Length=168, Percent_Identity=34.5238095238095, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17507255, Length=243, Percent_Identity=29.6296296296296, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324195, Length=245, Percent_Identity=26.9387755102041, Blast_Score=63, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6319945, Length=227, Percent_Identity=29.0748898678414, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR014182
- InterPro:   IPR011032
- InterPro:   IPR016040
- InterPro:   IPR002364 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 35826; Mature: 35826

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01162 QOR_ZETA_CRYSTAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIGLTRYLPISDPQSLVDVEIAQPEASGHDLLVKVEAISVNPVDTKVRAPKDKVEDAP
CCCCCCEEEECCCCCCCEEEEEEECCCCCCCEEEEEEEEEEECCCCCCCCCCHHHHCCCC
RVLGWDAAGTVVAVGADVTLFKVGDPVFYAGSITRPGANSEYHLVDERIVGHKPASLDFP
CEEECCCCCCEEEECCCEEEEEECCCEEEECCCCCCCCCCCEEEEHHHHHCCCCCCCCCC
QAAALPLTAITAWEAMFDRLRISPKGEDAGKSVLIIGGAGGVGSIGIQLAKVLAGLTVIA
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCEEEEE
TASRPESEAWCRGLGADHTIDHHGDIPAQLRALGFAHVDFVLCFNDTDRHFNAMAEAIAP
ECCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHCEEEEEEEEEECCCCHHHHHHHHHHCC
QGTICTIVENTGPLAISALKNKSAGFVWELMFTRAMFQTPDIIAQHRLLNEVARLIDAGK
CCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
LRTTVGDNLGTINAANLRRAHALLEGGRTIGKLVLSGF
EEECCCCCCCCEEHHHHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MKAIGLTRYLPISDPQSLVDVEIAQPEASGHDLLVKVEAISVNPVDTKVRAPKDKVEDAP
CCCCCCEEEECCCCCCCEEEEEEECCCCCCCEEEEEEEEEEECCCCCCCCCCHHHHCCCC
RVLGWDAAGTVVAVGADVTLFKVGDPVFYAGSITRPGANSEYHLVDERIVGHKPASLDFP
CEEECCCCCCEEEECCCEEEEEECCCEEEECCCCCCCCCCCEEEEHHHHHCCCCCCCCCC
QAAALPLTAITAWEAMFDRLRISPKGEDAGKSVLIIGGAGGVGSIGIQLAKVLAGLTVIA
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCEEEEE
TASRPESEAWCRGLGADHTIDHHGDIPAQLRALGFAHVDFVLCFNDTDRHFNAMAEAIAP
ECCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHCEEEEEEEEEECCCCHHHHHHHHHHCC
QGTICTIVENTGPLAISALKNKSAGFVWELMFTRAMFQTPDIIAQHRLLNEVARLIDAGK
CCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
LRTTVGDNLGTINAANLRRAHALLEGGRTIGKLVLSGF
EEECCCCCCCCEEHHHHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.26 {1,4-naphthoquinone}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]