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The map label for this gene is leuC [H]

Identifier: 94313199

GI number: 94313199

Start: 863774

End: 865207

Strand: Direct

Name: leuC [H]

Synonym: Rmet_4273

Alternate gene names: 94313199

Gene position: 863774-865207 (Clockwise)

Preceding gene: 94313198

Following gene: 94313200

Centisome position: 33.48

GC content: 64.09

Gene sequence:

>1434_bases
ATGGTGCAAGCCAATCCACCAGCCAGCCTGCTCGACAAGCTGTGGGCCAGCCACGTGATTCACGAGCAGTCCGACGGACC
TTCGCTGATCTATATCGACCGGCACCTGGTCAATGAGGTAACCAGCCCACAGGCCTTCGAGGGGCTGCGCCTGCATGGCC
GTGAGGTATGGCGTCCTGCATCGGTGCTGGCCACGGCCGACCACAACGTGCCAACTACCGAACGGGCGGCGGGCATTGCC
GACCCGATCAGCCGCGCGCAGGTCGACGCACTCGAGCGCAACTGCGCTGCGACTGGAATCGTGAACTTCGGCATGAACGA
CCCGCGCCAGGGCATTCTGCACGTGGTGGGTCCCGAGCGTGGCGCGACGCTGCCCGGTATGACCATTGTCTGCGGCGACT
CACATACATCCACACATGGCGCCTTCGGAGCCTTCGCTTTCGGTATCGGTACCTCGGAAGTCGAACAGGTGCTCAGCGCG
CAGTGTCTGTGGATGAAAAAGCCGCGCTCGATGCTGGTCCGGGTAGAAGGTGAGTTGGCGGCAGGTGTTTCAGCCAAGGA
CATCGCGCTGGCGCTGATCGGACGCATTGGTACGGCGGGAGCGACCGGGTACGTGATCGAGTTCGCCGGCAGCACCATCC
GTGGCCTCAGTATGGAAGGGCGCATGACCGTCTGCAACATGGCGATCGAGGCGGGGGCTCGCGCTGGCATGGTGGCCGTG
GACGAAACCACGTTGGCATACTTGGCCCATCGCCCGCAGGCGCCGCAAGGTGAAGCATGGGAAAGTGCCAGCGCCTACTG
GCGCACGCTGCGCAGCGATCCGGACGCAGTGTTCGACGCGGTCGTGGACATTGGTGTTGCGACGATCCGGCCGCATGTGA
CATGGGGCACGTCCCCGGAAATGGTGGTGGCGATCGACGAGCGCATTCCCGATCCGCGACAGGAAGCCGATCCGGTCAGG
CGCCGTGGCATGGAACGGGCACTAACCTACATGGGGCTGGAGCCCGGCATCAAAGTGGCATCTATCGCGCTCGATAAGGT
TTTCATCGGGTCGTGCACCAATGCACGGCTCGAGGACTTGCGAGCCGCCGCCGACGTGCTGCGCGGCCGCCACGTGGCGT
CGAACATTCGTCAGGCGCTAGTGGTGCCCGGCTCCGGGCTGGTGAAGTCCCAAGCGGAAGCGGAAGGCCTTGACAGGGTT
TTCATCGACGCTGGCTTCGAGTGGCGTGAGCCTGGATGCTCAATGTGCCTGGGCATGAATGACGATCGTCTTGCTCCTGG
TGAGCGCTGCGCATCCACCTCAAATCGCAACTTCGAAGGCAGGCAGGGGCCCGGTGGTCGCACCCACCTGGTCAGCCCGC
AAATGGCCGCGGCAGCGGCGGTTGCCGGGCACTTTGTCGACGTAAGCACCGTGCTGCAGGAGAAGTCAGCGTGA

Upstream 100 bases:

>100_bases
TGCTCAAGGCCGGCAAGCGCTCCGAACTCCATCCCGCGCCGCCTGTCGGCGCGGGGCGATGACCTGATGACCATGACCTG
ACCAAGAGGAGACCATAAGC

Downstream 100 bases:

>100_bases
AGGCCTTCACAGTACTCGATGGGCTGGTGGCGCCTATTGACCGTGCCAATGTGGATACGGACGCCATCTTGCCGAAGCAA
TTCTTGAAATCCATCCAGCG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MVQANPPASLLDKLWASHVIHEQSDGPSLIYIDRHLVNEVTSPQAFEGLRLHGREVWRPASVLATADHNVPTTERAAGIA
DPISRAQVDALERNCAATGIVNFGMNDPRQGILHVVGPERGATLPGMTIVCGDSHTSTHGAFGAFAFGIGTSEVEQVLSA
QCLWMKKPRSMLVRVEGELAAGVSAKDIALALIGRIGTAGATGYVIEFAGSTIRGLSMEGRMTVCNMAIEAGARAGMVAV
DETTLAYLAHRPQAPQGEAWESASAYWRTLRSDPDAVFDAVVDIGVATIRPHVTWGTSPEMVVAIDERIPDPRQEADPVR
RRGMERALTYMGLEPGIKVASIALDKVFIGSCTNARLEDLRAAADVLRGRHVASNIRQALVVPGSGLVKSQAEAEGLDRV
FIDAGFEWREPGCSMCLGMNDDRLAPGERCASTSNRNFEGRQGPGGRTHLVSPQMAAAAAVAGHFVDVSTVLQEKSA

Sequences:

>Translated_477_residues
MVQANPPASLLDKLWASHVIHEQSDGPSLIYIDRHLVNEVTSPQAFEGLRLHGREVWRPASVLATADHNVPTTERAAGIA
DPISRAQVDALERNCAATGIVNFGMNDPRQGILHVVGPERGATLPGMTIVCGDSHTSTHGAFGAFAFGIGTSEVEQVLSA
QCLWMKKPRSMLVRVEGELAAGVSAKDIALALIGRIGTAGATGYVIEFAGSTIRGLSMEGRMTVCNMAIEAGARAGMVAV
DETTLAYLAHRPQAPQGEAWESASAYWRTLRSDPDAVFDAVVDIGVATIRPHVTWGTSPEMVVAIDERIPDPRQEADPVR
RRGMERALTYMGLEPGIKVASIALDKVFIGSCTNARLEDLRAAADVLRGRHVASNIRQALVVPGSGLVKSQAEAEGLDRV
FIDAGFEWREPGCSMCLGMNDDRLAPGERCASTSNRNFEGRQGPGGRTHLVSPQMAAAAAVAGHFVDVSTVLQEKSA
>Mature_477_residues
MVQANPPASLLDKLWASHVIHEQSDGPSLIYIDRHLVNEVTSPQAFEGLRLHGREVWRPASVLATADHNVPTTERAAGIA
DPISRAQVDALERNCAATGIVNFGMNDPRQGILHVVGPERGATLPGMTIVCGDSHTSTHGAFGAFAFGIGTSEVEQVLSA
QCLWMKKPRSMLVRVEGELAAGVSAKDIALALIGRIGTAGATGYVIEFAGSTIRGLSMEGRMTVCNMAIEAGARAGMVAV
DETTLAYLAHRPQAPQGEAWESASAYWRTLRSDPDAVFDAVVDIGVATIRPHVTWGTSPEMVVAIDERIPDPRQEADPVR
RRGMERALTYMGLEPGIKVASIALDKVFIGSCTNARLEDLRAAADVLRGRHVASNIRQALVVPGSGLVKSQAEAEGLDRV
FIDAGFEWREPGCSMCLGMNDDRLAPGERCASTSNRNFEGRQGPGGRTHLVSPQMAAAAAVAGHFVDVSTVLQEKSA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=380, Percent_Identity=26.5789473684211, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI8659555, Length=442, Percent_Identity=26.2443438914027, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI41352693, Length=380, Percent_Identity=26.5789473684211, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1786259, Length=463, Percent_Identity=60.9071274298056, Blast_Score=589, Evalue=1e-169,
Organism=Escherichia coli, GI1787531, Length=361, Percent_Identity=25.207756232687, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081781, Length=322, Percent_Identity=25.776397515528, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI25149337, Length=435, Percent_Identity=26.6666666666667, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=435, Percent_Identity=26.6666666666667, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25149342, Length=326, Percent_Identity=26.9938650306748, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=466, Percent_Identity=24.6781115879828, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=468, Percent_Identity=60.042735042735, Blast_Score=573, Evalue=1e-164,
Organism=Saccharomyces cerevisiae, GI6323335, Length=391, Percent_Identity=28.3887468030691, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6322261, Length=385, Percent_Identity=27.2727272727273, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6320440, Length=419, Percent_Identity=25.5369928400955, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI281365315, Length=393, Percent_Identity=26.4631043256997, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI17864292, Length=393, Percent_Identity=26.4631043256997, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI161076999, Length=393, Percent_Identity=26.4631043256997, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI28571643, Length=476, Percent_Identity=25.2100840336134, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24645686, Length=447, Percent_Identity=25.503355704698, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17137564, Length=374, Percent_Identity=26.7379679144385, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50794; Mature: 50794

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQANPPASLLDKLWASHVIHEQSDGPSLIYIDRHLVNEVTSPQAFEGLRLHGREVWRPA
CCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHCCCCHHHHHHHHCCHHHCCCH
SVLATADHNVPTTERAAGIADPISRAQVDALERNCAATGIVNFGMNDPRQGILHVVGPER
HHEEECCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEECCCC
GATLPGMTIVCGDSHTSTHGAFGAFAFGIGTSEVEQVLSAQCLWMKKPRSMLVRVEGELA
CCCCCCEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHH
AGVSAKDIALALIGRIGTAGATGYVIEFAGSTIRGLSMEGRMTVCNMAIEAGARAGMVAV
CCCCHHHHHHHHHHHCCCCCCCCEEEEECCCEEECCCCCCCCHHHHHHHHCCCCCCEEEE
DETTLAYLAHRPQAPQGEAWESASAYWRTLRSDPDAVFDAVVDIGVATIRPHVTWGTSPE
CHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCHHHCCCEEEECCCCC
MVVAIDERIPDPRQEADPVRRRGMERALTYMGLEPGIKVASIALDKVFIGSCTNARLEDL
EEEEECCCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCEEEHEEHHHHEECCCCCHHHHHH
RAAADVLRGRHVASNIRQALVVPGSGLVKSQAEAEGLDRVFIDAGFEWREPGCSMCLGMN
HHHHHHHHCHHHHHHHHHEEECCCCCCCCCCHHHCCCCEEEEECCCCCCCCCCCEEECCC
DDRLAPGERCASTSNRNFEGRQGPGGRTHLVSPQMAAAAAVAGHFVDVSTVLQEKSA
CCCCCCCHHHCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVQANPPASLLDKLWASHVIHEQSDGPSLIYIDRHLVNEVTSPQAFEGLRLHGREVWRPA
CCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHCCCCHHHHHHHHCCHHHCCCH
SVLATADHNVPTTERAAGIADPISRAQVDALERNCAATGIVNFGMNDPRQGILHVVGPER
HHEEECCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEECCCC
GATLPGMTIVCGDSHTSTHGAFGAFAFGIGTSEVEQVLSAQCLWMKKPRSMLVRVEGELA
CCCCCCEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHH
AGVSAKDIALALIGRIGTAGATGYVIEFAGSTIRGLSMEGRMTVCNMAIEAGARAGMVAV
CCCCHHHHHHHHHHHCCCCCCCCEEEEECCCEEECCCCCCCCHHHHHHHHCCCCCCEEEE
DETTLAYLAHRPQAPQGEAWESASAYWRTLRSDPDAVFDAVVDIGVATIRPHVTWGTSPE
CHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCHHHCCCEEEECCCCC
MVVAIDERIPDPRQEADPVRRRGMERALTYMGLEPGIKVASIALDKVFIGSCTNARLEDL
EEEEECCCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCEEEHEEHHHHEECCCCCHHHHHH
RAAADVLRGRHVASNIRQALVVPGSGLVKSQAEAEGLDRVFIDAGFEWREPGCSMCLGMN
HHHHHHHHCHHHHHHHHHEEECCCCCCCCCCHHHCCCCEEEEECCCCCCCCCCCEEECCC
DDRLAPGERCASTSNRNFEGRQGPGGRTHLVSPQMAAAAAVAGHFVDVSTVLQEKSA
CCCCCCCHHHCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA