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The map label for this gene is leuD [H]

Identifier: 94313200

GI number: 94313200

Start: 865204

End: 865842

Strand: Direct

Name: leuD [H]

Synonym: Rmet_4274

Alternate gene names: 94313200

Gene position: 865204-865842 (Clockwise)

Preceding gene: 94313199

Following gene: 94313201

Centisome position: 33.53

GC content: 59.15

Gene sequence:

>639_bases
GTGAAGGCCTTCACAGTACTCGATGGGCTGGTGGCGCCTATTGACCGTGCCAATGTGGATACGGACGCCATCTTGCCGAA
GCAATTCTTGAAATCCATCCAGCGCAGCGGATTCGGTCCGTATCTGTTCGACGAGTGGCGCTATCTCGACCCTGGCGAGC
CAGGGCAGGACTGTTCGCAGCGGCCGCGCAATCCTGATTTCGTGCTTAACCAGCCGCGATACCAAGGCGCTTCGGTGCTG
TTGGTGCGCGAAAATTTCGGTTGCGGATCGTCGCGCGAGCATGCGCCATGGGCCCTTGAAGACTTCGGGCTACGCGCGCT
GATCGCCCCCAGCTTTGCGGACATCTTCTGCAACAACTGCATGAAGAATGGCCTGCTGCCGATCGTGCTGGAGGCTAACG
TCGTTGACAGGCTTTTCGCTGAGGTCGACGCCACGCCGGGATACCGCCTGCATGTCGATCTGGCAACACAACTGGTGACG
ACACCCTCGGGCGACGCCATTTCTTTCTCGATGGATCCATTCAAGAAGCACTGCGTGCTGAATGGCTTGGATGACATCGG
GCTGGCGCTGCAGCACGAGGAGGCGATCCGTCGCTACGAGCTGAACAGAATCAGGCACGAGCCCTGGCTGTTCCTATGA

Upstream 100 bases:

>100_bases
CCGGTGGTCGCACCCACCTGGTCAGCCCGCAAATGGCCGCGGCAGCGGCGGTTGCCGGGCACTTTGTCGACGTAAGCACC
GTGCTGCAGGAGAAGTCAGC

Downstream 100 bases:

>100_bases
GCGGGCGCAAGCCACTCATGTCCCACACCCACATTTACAAGAAATCAGGAGACACCATATGCATGCCCATCGTACGACCG
CGAGGTTTTCTGCTGCTGCG

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MKAFTVLDGLVAPIDRANVDTDAILPKQFLKSIQRSGFGPYLFDEWRYLDPGEPGQDCSQRPRNPDFVLNQPRYQGASVL
LVRENFGCGSSREHAPWALEDFGLRALIAPSFADIFCNNCMKNGLLPIVLEANVVDRLFAEVDATPGYRLHVDLATQLVT
TPSGDAISFSMDPFKKHCVLNGLDDIGLALQHEEAIRRYELNRIRHEPWLFL

Sequences:

>Translated_212_residues
MKAFTVLDGLVAPIDRANVDTDAILPKQFLKSIQRSGFGPYLFDEWRYLDPGEPGQDCSQRPRNPDFVLNQPRYQGASVL
LVRENFGCGSSREHAPWALEDFGLRALIAPSFADIFCNNCMKNGLLPIVLEANVVDRLFAEVDATPGYRLHVDLATQLVT
TPSGDAISFSMDPFKKHCVLNGLDDIGLALQHEEAIRRYELNRIRHEPWLFL
>Mature_212_residues
MKAFTVLDGLVAPIDRANVDTDAILPKQFLKSIQRSGFGPYLFDEWRYLDPGEPGQDCSQRPRNPDFVLNQPRYQGASVL
LVRENFGCGSSREHAPWALEDFGLRALIAPSFADIFCNNCMKNGLLPIVLEANVVDRLFAEVDATPGYRLHVDLATQLVT
TPSGDAISFSMDPFKKHCVLNGLDDIGLALQHEEAIRRYELNRIRHEPWLFL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=202, Percent_Identity=56.9306930693069, Blast_Score=228, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6321429, Length=216, Percent_Identity=50.462962962963, Blast_Score=208, Evalue=5e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 23859; Mature: 23859

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFTVLDGLVAPIDRANVDTDAILPKQFLKSIQRSGFGPYLFDEWRYLDPGEPGQDCSQ
CCHHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCHHHH
RPRNPDFVLNQPRYQGASVLLVRENFGCGSSREHAPWALEDFGLRALIAPSFADIFCNNC
CCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHCCCEEEECCHHHHHHHHHH
MKNGLLPIVLEANVVDRLFAEVDATPGYRLHVDLATQLVTTPSGDAISFSMDPFKKHCVL
HHCCCEEEEEECHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCEEEEECCHHHHHHHH
NGLDDIGLALQHEEAIRRYELNRIRHEPWLFL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKAFTVLDGLVAPIDRANVDTDAILPKQFLKSIQRSGFGPYLFDEWRYLDPGEPGQDCSQ
CCHHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCHHHH
RPRNPDFVLNQPRYQGASVLLVRENFGCGSSREHAPWALEDFGLRALIAPSFADIFCNNC
CCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHCCCEEEECCHHHHHHHHHH
MKNGLLPIVLEANVVDRLFAEVDATPGYRLHVDLATQLVTTPSGDAISFSMDPFKKHCVL
HHCCCEEEEEECHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCEEEEECCHHHHHHHH
NGLDDIGLALQHEEAIRRYELNRIRHEPWLFL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA