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The map label for this gene is plcN [H]

Identifier: 94313119

GI number: 94313119

Start: 775234

End: 777378

Strand: Reverse

Name: plcN [H]

Synonym: Rmet_4192

Alternate gene names: 94313119

Gene position: 777378-775234 (Counterclockwise)

Preceding gene: 291481518

Following gene: 94313118

Centisome position: 30.13

GC content: 66.34

Gene sequence:

>2145_bases
ATGAACTCCAGCAGCCGACGCAATTTCCTGAAGATGGCCGGCAGCAGTGCCGCCGCCACCGCCGCGCTTGCCGCATTCCC
GCCGGCCATCCGCCGCGCGCTGGCCATTCCGGCCAACAACGCTACCAAATCGATCCGCGACGTCGAATACGTTGTGATCC
TGACGCAGGAGAATCGCTCGTTCGATCACTACTTCGGCACCATGAACGGCGTGCGCGGCTTCAGCGACCGCTTCCCGATT
CCACTGCCGGGCGGCCGCAACGCATTCCAGCAGACCTACGCCAGCAACAACATCAACCGCGTGGTTCTGCCGTATCACCT
GGACCAGACCGCCGGCAACGCCCAGCGCGTGAGCGGCACGCCTCACAGCCAGCCCGACGCGCAGGCCGCGTGGGACCTCG
GCCGCATGAGCGCGTGGCCCACCGCCAAGAAGACCCAGTCGATGGGCTACTACACCGAGCAGGAACTGGGCTTCCAGTAC
GCGCTGGCCAATGCCTTCACGCTGTGCGACGCCTACCACTGCGGCTTCCACGGTGGCACCAATACGAACCGGCTGTTCCA
GTGGACCGGCACCAATGATCCGTTTGGCAAGAACGGCGGCCCGGTGATCGACAACAGCGGCGACTCGCTCGACGCCACCG
GCGTCAACTACACCTGGACCACCTATCCGGAACGCCTGCAGGCCGCCGGCGTGACCTGGAAGGTCTACCAGAACATGCCG
GACAACTTCACCGACAATCCGCTGGCTGGCTTCAAGCAGTACCGCGCGGCCAACGCGGCGCTGGGCAATGCCGCCAACGG
CTCGCCGTACACGCCGTATACGCCGGCCAACGACACGGTGAACCCGCTCTTCAAGGGCATCGGCAACACCATGCCCGATG
GCGGCTTCCTGCAGGCACTGCGCGATGACATCGCCGCCGGCACGCTGCCGCAGGTATCGTGGATCGTCGCACCTGCGACA
TACTCGGAACACCCGGGGCCGTCGAGCCCGGTGCAGGGCGCGTGGTACACGCAGCAGTTGCTCGACGCGCTGACGGCCAA
CCCCGCGATCTGGAGCCGCACGGTCCTGCTGATCAACTTCGACGAGAACGACGGCTTCTTCGACCACGTGCCGCCGCCGT
GCGCCCCCGCGCTCGACGCTACGGGCAACCCCGTCGGCTACACGACGATGGATGCATCGACCGAGTACTACAGCGTCGAC
AAGACGCCGTTTGGTCCCGGCCCGCGCGTGCCGATGTACGTTGTGTCGCCGTGGAGCCGAGGTGGCTGGGTCAACTCGCA
GGCCTTCGATCACACCTCGATCCTGCGTTTCATCGAGCAGCGCTTCGGCGTGGCGGAAACCAACATCAGTGCCTACCGCC
GCGCGATCATGGGCGATCTGATGTCGGCGTTCGACTTCATCAACCCGAACAGCAACACGGCCCTGGCCTTCACGCCGTTG
CAGAAGACGGACGCCGACACGCTGCGCGCCGCGCAGGATGCCAAGGCGCAGATTCCTGCGCCGACGGTTGCCGCCCAGTC
GATGCCCACCCAGAAGAACGGCACACGCCCGTCGCGCGCGCTGCCCTACACGCTGCATACCAGCGGCTTCGAGGACCCGT
CCACGAACACGGTCTGGCTGCGTTTCAAGAACGACGGCACCCAGGCCGCGGTATTCCACGTGTACGACCGCCTGCATCTG
GGCGATGTGCCGCGCCGCTATGCGATCGAGGCGGGCAAGTCCTACGACGCGAAGCTCGACGTGAGCCGCGACAGTGGCCG
CTACGACCTCTGGGTGCTTGGCCCGAATGGCTATCACCGCGCGTTCGTCGGCGATATCAGCGCGCAGAAGGCTGCCGGCG
GCGGTGCCGCGCCCGAGATCCGTGTGTGCTACGACGAGGCCAACGCCCAGGTCTGGCTCACGCTGATCAACCGCGGCAGC
GCGACGTGCACGTTCACGGTCAAGCCGAACGCCTATCGGAACGACGGCCCCTGGACGTTCGAGGTCCCGGCCGGCAAGGA
GGTCGACCAGCACTGGCCCGTGGGGTCGCAAGGCAACTGGTACGACTTCACGGTCACCACACAGCAGGGTGGTTTCATGC
GCCGCTTCGCCGGCCGGCTGGAGAACGGCACGCATACGGTCTCCGACCCGGCCATGGGCGCCTGA

Upstream 100 bases:

>100_bases
CGGCGATACTCCCGATTCCGCCAAGCCGCTCATGAAATGCGCGCCCTGAGCACCGATGGACGCCACCAACAACGACACGC
TTGACCAGGGCAGAACCGCT

Downstream 100 bases:

>100_bases
CAACGGCTCACCGCAGCTGGTCCGGCAGCCTCGCAGTTGCCGGGCCGGTGAGCCGCCCGGCCAGATAGTCGCTCATCCGG
GGATGCCCACTGCATCCCCG

Product: phospholipase C (phosphatidylcholine cholinephosphohydrolase) signal peptide protein

Products: NA

Alternate protein names: PLC-N; Phosphatidylcholine cholinephosphohydrolase; Phosphatidylcholine-hydrolyzing phospholipase C; PC-PLC [H]

Number of amino acids: Translated: 714; Mature: 714

Protein sequence:

>714_residues
MNSSSRRNFLKMAGSSAAATAALAAFPPAIRRALAIPANNATKSIRDVEYVVILTQENRSFDHYFGTMNGVRGFSDRFPI
PLPGGRNAFQQTYASNNINRVVLPYHLDQTAGNAQRVSGTPHSQPDAQAAWDLGRMSAWPTAKKTQSMGYYTEQELGFQY
ALANAFTLCDAYHCGFHGGTNTNRLFQWTGTNDPFGKNGGPVIDNSGDSLDATGVNYTWTTYPERLQAAGVTWKVYQNMP
DNFTDNPLAGFKQYRAANAALGNAANGSPYTPYTPANDTVNPLFKGIGNTMPDGGFLQALRDDIAAGTLPQVSWIVAPAT
YSEHPGPSSPVQGAWYTQQLLDALTANPAIWSRTVLLINFDENDGFFDHVPPPCAPALDATGNPVGYTTMDASTEYYSVD
KTPFGPGPRVPMYVVSPWSRGGWVNSQAFDHTSILRFIEQRFGVAETNISAYRRAIMGDLMSAFDFINPNSNTALAFTPL
QKTDADTLRAAQDAKAQIPAPTVAAQSMPTQKNGTRPSRALPYTLHTSGFEDPSTNTVWLRFKNDGTQAAVFHVYDRLHL
GDVPRRYAIEAGKSYDAKLDVSRDSGRYDLWVLGPNGYHRAFVGDISAQKAAGGGAAPEIRVCYDEANAQVWLTLINRGS
ATCTFTVKPNAYRNDGPWTFEVPAGKEVDQHWPVGSQGNWYDFTVTTQQGGFMRRFAGRLENGTHTVSDPAMGA

Sequences:

>Translated_714_residues
MNSSSRRNFLKMAGSSAAATAALAAFPPAIRRALAIPANNATKSIRDVEYVVILTQENRSFDHYFGTMNGVRGFSDRFPI
PLPGGRNAFQQTYASNNINRVVLPYHLDQTAGNAQRVSGTPHSQPDAQAAWDLGRMSAWPTAKKTQSMGYYTEQELGFQY
ALANAFTLCDAYHCGFHGGTNTNRLFQWTGTNDPFGKNGGPVIDNSGDSLDATGVNYTWTTYPERLQAAGVTWKVYQNMP
DNFTDNPLAGFKQYRAANAALGNAANGSPYTPYTPANDTVNPLFKGIGNTMPDGGFLQALRDDIAAGTLPQVSWIVAPAT
YSEHPGPSSPVQGAWYTQQLLDALTANPAIWSRTVLLINFDENDGFFDHVPPPCAPALDATGNPVGYTTMDASTEYYSVD
KTPFGPGPRVPMYVVSPWSRGGWVNSQAFDHTSILRFIEQRFGVAETNISAYRRAIMGDLMSAFDFINPNSNTALAFTPL
QKTDADTLRAAQDAKAQIPAPTVAAQSMPTQKNGTRPSRALPYTLHTSGFEDPSTNTVWLRFKNDGTQAAVFHVYDRLHL
GDVPRRYAIEAGKSYDAKLDVSRDSGRYDLWVLGPNGYHRAFVGDISAQKAAGGGAAPEIRVCYDEANAQVWLTLINRGS
ATCTFTVKPNAYRNDGPWTFEVPAGKEVDQHWPVGSQGNWYDFTVTTQQGGFMRRFAGRLENGTHTVSDPAMGA
>Mature_714_residues
MNSSSRRNFLKMAGSSAAATAALAAFPPAIRRALAIPANNATKSIRDVEYVVILTQENRSFDHYFGTMNGVRGFSDRFPI
PLPGGRNAFQQTYASNNINRVVLPYHLDQTAGNAQRVSGTPHSQPDAQAAWDLGRMSAWPTAKKTQSMGYYTEQELGFQY
ALANAFTLCDAYHCGFHGGTNTNRLFQWTGTNDPFGKNGGPVIDNSGDSLDATGVNYTWTTYPERLQAAGVTWKVYQNMP
DNFTDNPLAGFKQYRAANAALGNAANGSPYTPYTPANDTVNPLFKGIGNTMPDGGFLQALRDDIAAGTLPQVSWIVAPAT
YSEHPGPSSPVQGAWYTQQLLDALTANPAIWSRTVLLINFDENDGFFDHVPPPCAPALDATGNPVGYTTMDASTEYYSVD
KTPFGPGPRVPMYVVSPWSRGGWVNSQAFDHTSILRFIEQRFGVAETNISAYRRAIMGDLMSAFDFINPNSNTALAFTPL
QKTDADTLRAAQDAKAQIPAPTVAAQSMPTQKNGTRPSRALPYTLHTSGFEDPSTNTVWLRFKNDGTQAAVFHVYDRLHL
GDVPRRYAIEAGKSYDAKLDVSRDSGRYDLWVLGPNGYHRAFVGDISAQKAAGGGAAPEIRVCYDEANAQVWLTLINRGS
ATCTFTVKPNAYRNDGPWTFEVPAGKEVDQHWPVGSQGNWYDFTVTTQQGGFMRRFAGRLENGTHTVSDPAMGA

Specific function: Hydrolyzes phosphatidylserine as well as phosphatidylcholine [H]

COG id: COG3511

COG function: function code M; Phospholipase C

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial phospholipase C family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007312
- InterPro:   IPR008475
- InterPro:   IPR017767
- InterPro:   IPR006311 [H]

Pfam domain/function: PF05506 DUF756; PF04185 Phosphoesterase [H]

EC number: =3.1.4.3 [H]

Molecular weight: Translated: 77823; Mature: 77823

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSSRRNFLKMAGSSAAATAALAAFPPAIRRALAIPANNATKSIRDVEYVVILTQENRS
CCCCHHHHHHHHCCCCHHHHHHHHHCCHHHHHEEECCCCCCHHHHCCEEEEEEEEECCCC
FDHYFGTMNGVRGFSDRFPIPLPGGRNAFQQTYASNNINRVVLPYHLDQTAGNAQRVSGT
HHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCC
PHSQPDAQAAWDLGRMSAWPTAKKTQSMGYYTEQELGFQYALANAFTLCDAYHCGFHGGT
CCCCCCHHHHHHHHCCCCCCCCHHHHCCCCEEHHHCCHHHHHHHHHHHHHHHCCCCCCCC
NTNRLFQWTGTNDPFGKNGGPVIDNSGDSLDATGVNYTWTTYPERLQAAGVTWKVYQNMP
CCCEEEEECCCCCCCCCCCCCEECCCCCCCCCCCCCEEECCCHHHHHHCCCEEEEECCCC
DNFTDNPLAGFKQYRAANAALGNAANGSPYTPYTPANDTVNPLFKGIGNTMPDGGFLQAL
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHH
RDDIAAGTLPQVSWIVAPATYSEHPGPSSPVQGAWYTQQLLDALTANPAIWSRTVLLINF
HHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEEE
DENDGFFDHVPPPCAPALDATGNPVGYTTMDASTEYYSVDKTPFGPGPRVPMYVVSPWSR
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCEEEECCCCC
GGWVNSQAFDHTSILRFIEQRFGVAETNISAYRRAIMGDLMSAFDFINPNSNTALAFTPL
CCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECC
QKTDADTLRAAQDAKAQIPAPTVAAQSMPTQKNGTRPSRALPYTLHTSGFEDPSTNTVWL
CCCCHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEE
RFKNDGTQAAVFHVYDRLHLGDVPRRYAIEAGKSYDAKLDVSRDSGRYDLWVLGPNGYHR
EECCCCCEEEEEEHHHHHHCCCCCHHHHHHCCCCCCCEEEECCCCCCEEEEEECCCCCCE
AFVGDISAQKAAGGGAAPEIRVCYDEANAQVWLTLINRGSATCTFTVKPNAYRNDGPWTF
EEEECCCHHHCCCCCCCCEEEEEEECCCCEEEEEEEECCCEEEEEEECCCCCCCCCCEEE
EVPAGKEVDQHWPVGSQGNWYDFTVTTQQGGFMRRFAGRLENGTHTVSDPAMGA
ECCCCCCCHHCCCCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCEECCCCCCCC
>Mature Secondary Structure
MNSSSRRNFLKMAGSSAAATAALAAFPPAIRRALAIPANNATKSIRDVEYVVILTQENRS
CCCCHHHHHHHHCCCCHHHHHHHHHCCHHHHHEEECCCCCCHHHHCCEEEEEEEEECCCC
FDHYFGTMNGVRGFSDRFPIPLPGGRNAFQQTYASNNINRVVLPYHLDQTAGNAQRVSGT
HHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCC
PHSQPDAQAAWDLGRMSAWPTAKKTQSMGYYTEQELGFQYALANAFTLCDAYHCGFHGGT
CCCCCCHHHHHHHHCCCCCCCCHHHHCCCCEEHHHCCHHHHHHHHHHHHHHHCCCCCCCC
NTNRLFQWTGTNDPFGKNGGPVIDNSGDSLDATGVNYTWTTYPERLQAAGVTWKVYQNMP
CCCEEEEECCCCCCCCCCCCCEECCCCCCCCCCCCCEEECCCHHHHHHCCCEEEEECCCC
DNFTDNPLAGFKQYRAANAALGNAANGSPYTPYTPANDTVNPLFKGIGNTMPDGGFLQAL
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHH
RDDIAAGTLPQVSWIVAPATYSEHPGPSSPVQGAWYTQQLLDALTANPAIWSRTVLLINF
HHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEEE
DENDGFFDHVPPPCAPALDATGNPVGYTTMDASTEYYSVDKTPFGPGPRVPMYVVSPWSR
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCEEEECCCCC
GGWVNSQAFDHTSILRFIEQRFGVAETNISAYRRAIMGDLMSAFDFINPNSNTALAFTPL
CCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECC
QKTDADTLRAAQDAKAQIPAPTVAAQSMPTQKNGTRPSRALPYTLHTSGFEDPSTNTVWL
CCCCHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEE
RFKNDGTQAAVFHVYDRLHLGDVPRRYAIEAGKSYDAKLDVSRDSGRYDLWVLGPNGYHR
EECCCCCEEEEEEHHHHHHCCCCCHHHHHHCCCCCCCEEEECCCCCCEEEEEECCCCCCE
AFVGDISAQKAAGGGAAPEIRVCYDEANAQVWLTLINRGSATCTFTVKPNAYRNDGPWTF
EEEECCCHHHCCCCCCCCEEEEEEECCCCEEEEEEEECCCEEEEEEECCCCCCCCCCEEE
EVPAGKEVDQHWPVGSQGNWYDFTVTTQQGGFMRRFAGRLENGTHTVSDPAMGA
ECCCCCCCHHCCCCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2120196; 10984043 [H]