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The map label for this gene is phoA1 [H]

Identifier: 94313011

GI number: 94313011

Start: 651730

End: 653190

Strand: Direct

Name: phoA1 [H]

Synonym: Rmet_4084

Alternate gene names: 94313011

Gene position: 651730-653190 (Clockwise)

Preceding gene: 94313007

Following gene: 94313012

Centisome position: 25.26

GC content: 64.07

Gene sequence:

>1461_bases
ATGAGAAGGATTTCCGTACTCGCGGCAGCCGCCATGTCGGTCGCCCTCGCGGCCTGCGGCGGCGATGACAACAATGGCAA
CAACGCCGGCACGACGCCGCCGATCGAGCAGCCACAAGGCGGCACCACGCCGACCAAGAACGTCGTATTTTTCCTCGGCG
ACGGCATGGGCATGACGACGTTGACCGCCGCGCGTATCTACAAGGTGGGCGAGGAAGGCGATCTGACGATCGACACGATG
CCGGAAAGCGGCTTCGTCCGTACGTATTCGAACGACGCCCAGGTCACGGACAGCGCCCCGTCGATGGCCGCCTACATGAC
CGGCGTGAAAATGAACAACGAAGTCATCTCGATGACTCCGGAAACGAGCGCCGTGGACGCCAACGGCAAGGCCTATGTGT
CGGGCGCTGACAGCACCTGCCCGGCGACGGGCAACGGCAAGAGCGTGCCGACGCTGCTCGAGCAGATGAAGGCTGCCGGC
TACGGCACGGGCGTGGTCACTACCACGCGCATCACGCACGCCACGCCGGCGGCCACCTACGCCCACATCTGCCACCGCGA
CGGCGAGAACGCCATCGCAGCGCAACTGGTACCGAAGGGCGCCGGCTTCAACGGCGCGCTGACCGATGGCGTCGACGTGA
TCTTCGGCGGCGGCCGCCAGCACTTCCTGGCCACGACCGCAGGCGGCAAGCGTACCGATGGCCGCGATCTGGTCGGCGAG
TTCAAGACGGCCGGCTATAGCTACGCTGCCAGCAAGTCGGACTTCGACAAGATCACGGCCAGCTCTGCCAAGGTGGTGGG
TCTGTTCAACGCGAGCCACATGGATTACGACCTCGATCGCGATCCGGCGAAGGAGCCGAGCCTGGCAGAAATGACCGGCA
AGGCCATCGACGTGCTGGCTGCCAAGAAGAAGGGCTTCTTCCTGATGGTGGAAGGCGGTCGTATCGACCACGCGCTGCAC
GAGACCACCGCCCGCAAGGCACTGCAGGACACCGCCGCGTTCGACGACGCGATCAAGGCTGCCATCGACAAGCTCAACGT
AATCGACCCGGGCCTGAAGAACACGCTGATCGTCGTCACCGCCGACCATGACCACACCCTGGTCCTGAACGGCTACGCCA
AGCGCACGGGCAAGACCAGCGATGCCAACCCCGGCGTGCTGGGTCTGGTCAAGGACTACGTGACTGGCTCTGCCAAGCTC
GATTCGACCGGCAACCCGTTCTCGATCATCGGCTTCGGCAATGGCGAGAACCGTCCGGCCACGCGTACCGCGCTGACCGA
TGTGCAGGTCTTCGACAAGACCTATCACCAGGAAGCGGTGATTCCGATGCCCGCGGGTGGTGAAACCCACGGTGGCACCG
ATGTGTTCATCGGCGCCATGGGCAACGGGGCCACCAGCTTCACCGGTGTGATGGACAACATCGAGGTGTTCGGCCTGATC
AAGAAGGCAATCGGACTCTGA

Upstream 100 bases:

>100_bases
TGGGTCAGCCAAGAATGTCTTTCATAAGAATCAAGTAGTAATCTTCCTGCCACATTTCTCATGCATCCTCCAGTCCGTCT
CAAACAGGGAGGACACCATA

Downstream 100 bases:

>100_bases
GCCAACCCGCCATCACCGCACAACGGAATATCGACATGAAAATCACTACCCGTATCGGAATGCTGGCGGCCGCGGCGGCC
AGCGCTTGCCTGACGATGCC

Product: alkaline phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MRRISVLAAAAMSVALAACGGDDNNGNNAGTTPPIEQPQGGTTPTKNVVFFLGDGMGMTTLTAARIYKVGEEGDLTIDTM
PESGFVRTYSNDAQVTDSAPSMAAYMTGVKMNNEVISMTPETSAVDANGKAYVSGADSTCPATGNGKSVPTLLEQMKAAG
YGTGVVTTTRITHATPAATYAHICHRDGENAIAAQLVPKGAGFNGALTDGVDVIFGGGRQHFLATTAGGKRTDGRDLVGE
FKTAGYSYAASKSDFDKITASSAKVVGLFNASHMDYDLDRDPAKEPSLAEMTGKAIDVLAAKKKGFFLMVEGGRIDHALH
ETTARKALQDTAAFDDAIKAAIDKLNVIDPGLKNTLIVVTADHDHTLVLNGYAKRTGKTSDANPGVLGLVKDYVTGSAKL
DSTGNPFSIIGFGNGENRPATRTALTDVQVFDKTYHQEAVIPMPAGGETHGGTDVFIGAMGNGATSFTGVMDNIEVFGLI
KKAIGL

Sequences:

>Translated_486_residues
MRRISVLAAAAMSVALAACGGDDNNGNNAGTTPPIEQPQGGTTPTKNVVFFLGDGMGMTTLTAARIYKVGEEGDLTIDTM
PESGFVRTYSNDAQVTDSAPSMAAYMTGVKMNNEVISMTPETSAVDANGKAYVSGADSTCPATGNGKSVPTLLEQMKAAG
YGTGVVTTTRITHATPAATYAHICHRDGENAIAAQLVPKGAGFNGALTDGVDVIFGGGRQHFLATTAGGKRTDGRDLVGE
FKTAGYSYAASKSDFDKITASSAKVVGLFNASHMDYDLDRDPAKEPSLAEMTGKAIDVLAAKKKGFFLMVEGGRIDHALH
ETTARKALQDTAAFDDAIKAAIDKLNVIDPGLKNTLIVVTADHDHTLVLNGYAKRTGKTSDANPGVLGLVKDYVTGSAKL
DSTGNPFSIIGFGNGENRPATRTALTDVQVFDKTYHQEAVIPMPAGGETHGGTDVFIGAMGNGATSFTGVMDNIEVFGLI
KKAIGL
>Mature_486_residues
MRRISVLAAAAMSVALAACGGDDNNGNNAGTTPPIEQPQGGTTPTKNVVFFLGDGMGMTTLTAARIYKVGEEGDLTIDTM
PESGFVRTYSNDAQVTDSAPSMAAYMTGVKMNNEVISMTPETSAVDANGKAYVSGADSTCPATGNGKSVPTLLEQMKAAG
YGTGVVTTTRITHATPAATYAHICHRDGENAIAAQLVPKGAGFNGALTDGVDVIFGGGRQHFLATTAGGKRTDGRDLVGE
FKTAGYSYAASKSDFDKITASSAKVVGLFNASHMDYDLDRDPAKEPSLAEMTGKAIDVLAAKKKGFFLMVEGGRIDHALH
ETTARKALQDTAAFDDAIKAAIDKLNVIDPGLKNTLIVVTADHDHTLVLNGYAKRTGKTSDANPGVLGLVKDYVTGSAKL
DSTGNPFSIIGFGNGENRPATRTALTDVQVFDKTYHQEAVIPMPAGGETHGGTDVFIGAMGNGATSFTGVMDNIEVFGLI
KKAIGL

Specific function: Specifically cleaves both streptomycin-6-phosphate and, more slowly, streptomycin-3''-phosphate during the biosynthesis of streptomycin [H]

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=467, Percent_Identity=33.8329764453961, Blast_Score=219, Evalue=5e-57,
Organism=Homo sapiens, GI94721246, Length=467, Percent_Identity=33.6188436830835, Blast_Score=218, Evalue=9e-57,
Organism=Homo sapiens, GI116734717, Length=472, Percent_Identity=34.1101694915254, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI157266292, Length=466, Percent_Identity=33.2618025751073, Blast_Score=214, Evalue=2e-55,
Organism=Homo sapiens, GI294660770, Length=457, Percent_Identity=34.1356673960613, Blast_Score=208, Evalue=1e-53,
Organism=Homo sapiens, GI294660772, Length=421, Percent_Identity=32.541567695962, Blast_Score=178, Evalue=1e-44,
Organism=Escherichia coli, GI48994877, Length=482, Percent_Identity=27.5933609958506, Blast_Score=139, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6320689, Length=342, Percent_Identity=34.2105263157895, Blast_Score=153, Evalue=6e-38,
Organism=Drosophila melanogaster, GI85725200, Length=461, Percent_Identity=33.1887201735358, Blast_Score=241, Evalue=1e-63,
Organism=Drosophila melanogaster, GI85815833, Length=461, Percent_Identity=33.1887201735358, Blast_Score=241, Evalue=1e-63,
Organism=Drosophila melanogaster, GI21355151, Length=468, Percent_Identity=36.3247863247863, Blast_Score=238, Evalue=9e-63,
Organism=Drosophila melanogaster, GI19921912, Length=490, Percent_Identity=35.1020408163265, Blast_Score=232, Evalue=4e-61,
Organism=Drosophila melanogaster, GI21355981, Length=448, Percent_Identity=35.2678571428571, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI21358067, Length=488, Percent_Identity=35.0409836065574, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI18859923, Length=443, Percent_Identity=34.7629796839729, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24585213, Length=483, Percent_Identity=34.3685300207039, Blast_Score=212, Evalue=5e-55,
Organism=Drosophila melanogaster, GI24651554, Length=477, Percent_Identity=33.5429769392034, Blast_Score=196, Evalue=3e-50,
Organism=Drosophila melanogaster, GI21355149, Length=462, Percent_Identity=32.9004329004329, Blast_Score=196, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24657827, Length=486, Percent_Identity=33.7448559670782, Blast_Score=193, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24657835, Length=486, Percent_Identity=32.5102880658436, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24657842, Length=490, Percent_Identity=32.0408163265306, Blast_Score=183, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24651556, Length=467, Percent_Identity=33.1905781584582, Blast_Score=180, Evalue=2e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.39 [H]

Molecular weight: Translated: 50449; Mature: 50449

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRISVLAAAAMSVALAACGGDDNNGNNAGTTPPIEQPQGGTTPTKNVVFFLGDGMGMTT
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHH
LTAARIYKVGEEGDLTIDTMPESGFVRTYSNDAQVTDSAPSMAAYMTGVKMNNEVISMTP
HHHHHHEEECCCCCEEEEECCCCCEEEEECCCCEECCCCCCHHHHHCCEEECCCEEEECC
ETSAVDANGKAYVSGADSTCPATGNGKSVPTLLEQMKAAGYGTGVVTTTRITHATPAATY
CCCEECCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHH
AHICHRDGENAIAAQLVPKGAGFNGALTDGVDVIFGGGRQHFLATTAGGKRTDGRDLVGE
HHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHH
FKTAGYSYAASKSDFDKITASSAKVVGLFNASHMDYDLDRDPAKEPSLAEMTGKAIDVLA
HHHCCEEEECCCCCHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCEEEEE
AKKKGFFLMVEGGRIDHALHETTARKALQDTAAFDDAIKAAIDKLNVIDPGLKNTLIVVT
ECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
ADHDHTLVLNGYAKRTGKTSDANPGVLGLVKDYVTGSAKLDSTGNPFSIIGFGNGENRPA
CCCCCEEEEECCHHCCCCCCCCCCCEEEHHHHHCCCCCEECCCCCCEEEEEECCCCCCCC
TRTALTDVQVFDKTYHQEAVIPMPAGGETHGGTDVFIGAMGNGATSFTGVMDNIEVFGLI
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHCHHHHHHH
KKAIGL
HHHHCC
>Mature Secondary Structure
MRRISVLAAAAMSVALAACGGDDNNGNNAGTTPPIEQPQGGTTPTKNVVFFLGDGMGMTT
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHH
LTAARIYKVGEEGDLTIDTMPESGFVRTYSNDAQVTDSAPSMAAYMTGVKMNNEVISMTP
HHHHHHEEECCCCCEEEEECCCCCEEEEECCCCEECCCCCCHHHHHCCEEECCCEEEECC
ETSAVDANGKAYVSGADSTCPATGNGKSVPTLLEQMKAAGYGTGVVTTTRITHATPAATY
CCCEECCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHH
AHICHRDGENAIAAQLVPKGAGFNGALTDGVDVIFGGGRQHFLATTAGGKRTDGRDLVGE
HHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHH
FKTAGYSYAASKSDFDKITASSAKVVGLFNASHMDYDLDRDPAKEPSLAEMTGKAIDVLA
HHHCCEEEECCCCCHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCEEEEE
AKKKGFFLMVEGGRIDHALHETTARKALQDTAAFDDAIKAAIDKLNVIDPGLKNTLIVVT
ECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
ADHDHTLVLNGYAKRTGKTSDANPGVLGLVKDYVTGSAKLDSTGNPFSIIGFGNGENRPA
CCCCCEEEEECCHHCCCCCCCCCCCEEEHHHHHCCCCCEECCCCCCEEEEEECCCCCCCC
TRTALTDVQVFDKTYHQEAVIPMPAGGETHGGTDVFIGAMGNGATSFTGVMDNIEVFGLI
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHCHHHHHHH
KKAIGL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1654502 [H]