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The map label for this gene is phoA2 [H]

Identifier: 94313012

GI number: 94313012

Start: 653226

End: 654629

Strand: Direct

Name: phoA2 [H]

Synonym: Rmet_4085

Alternate gene names: 94313012

Gene position: 653226-654629 (Clockwise)

Preceding gene: 94313011

Following gene: 94313013

Centisome position: 25.32

GC content: 64.53

Gene sequence:

>1404_bases
ATGAAAATCACTACCCGTATCGGAATGCTGGCGGCCGCGGCGGCCAGCGCTTGCCTGACGATGCCCGCGGCGCAAGCCGC
TGGCGAGGCCAAGAACGTCATCTTCTTCCTGGGCGACGGCATGGGCCCCGCCACCGTTACCGCATCGCGTATCTACAAGT
ACGGCGAGTCGGGCAAGCTGACGATGGAATCGCTGAAGCGCACCGCGCGCATCAAGACGTTCTCCAACGACGCGCAAACC
ACCGACAGCGCACCGTCGATGGCTGCCTACATGACCGGCGTGAAGATGAACAACGAGGTCATCTCGATGTCTCCGGACAC
GAAGGCGACCGCGCCGGGCACAGACGTCAACGGCAACAAGACCATCAACAACTGCCTGCCGACCAACGGTACTTCGGTGC
CGACGCTGCTGGAACTGGCCAAGGCGCGCGGCAAGGCAACCGGCGCTATCACCACGACCGAGCTGACGCACGCCACGCCG
GCCGCCACGTACTCCCACATCTGCCATCGCGATGCGCAGTACGACATCGCCGCGCAAGCGGTGCCGGGTGGCGCGGGTTT
CAATACGGCGCTGGGTGACGGTGTCGACGTGCTGATGGGCGGCGGACGCAACCACTGGACGCCGTTTGACGCCACGGCCA
ACAAGAAGGGTCGTGCCGATGGCCGCAATCTGCTGACGGAGTTTGCCGCCAAGGGCTACGCCGTGGCCGCCACGCGCGAC
CAGATGATGCAGGCCAGCGGCAACAAGCTGATTGGTCTGTTCAGCACGACGAGCCATCTCGAGTACGAGATCGACCGCGT
GGCGGGCAAGGGCGAAGGCGCTACCCAGCCGAGCCTGGCCGACATGACCGCCAAGGCGATCGAGCTGCTGTCGAAGAATG
GCAATGGCTACTTCCTGATGGTCGAGGGTGGCCGTATCGACCACGCGCTGCACGGCACCAACGCCAAGCGTGCGCTGGAA
GACACCATCGCCTTCGACGACGCCATCAAGCGCGCGCTTTCGATGGTTGATCTGTCGAACACGATCATCGTGGTCACCGC
CGACCACGATCACACGATGACGATCAACGGCTACTCGCACCGCGGCAACCCGATCCTGGCCACGGCCACCGACTACAAGA
CCAAGAAGACCGCCCTGGCGGCCGACGGTCTGCCCTACACGACGCTGGTGTTCGGCAACGGTGGCACCCCGCGCAAGGCT
ACCCGCGACAACCCGTCGCTGGTGGACACCACCGCCAATGACTATCTGCAGGAAGTTGGCGTGAACCTGGGTTCCCCGGG
TGCCGAAACGCACGGTGGCGGCGATGTGATGCTGTTCTCCACCGGCCCCGGCAGCGCGCCGTTGAAGGGCACCCTCGACA
ACACCAAGGTGTTCGGTGTGGTCAAGTCGGCCCTGGGTCTCTGA

Upstream 100 bases:

>100_bases
GCTTCACCGGTGTGATGGACAACATCGAGGTGTTCGGCCTGATCAAGAAGGCAATCGGACTCTGAGCCAACCCGCCATCA
CCGCACAACGGAATATCGAC

Downstream 100 bases:

>100_bases
TCCTCGTCGTACCCGACACGGGCGCTTCGCGTGCCCGTGCCATGACGCACGCCGGTTCGCCGGCGTGCGCTTTCCACTTT
CAGCACGGCAATGATCAACA

Product: alkaline phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MKITTRIGMLAAAAASACLTMPAAQAAGEAKNVIFFLGDGMGPATVTASRIYKYGESGKLTMESLKRTARIKTFSNDAQT
TDSAPSMAAYMTGVKMNNEVISMSPDTKATAPGTDVNGNKTINNCLPTNGTSVPTLLELAKARGKATGAITTTELTHATP
AATYSHICHRDAQYDIAAQAVPGGAGFNTALGDGVDVLMGGGRNHWTPFDATANKKGRADGRNLLTEFAAKGYAVAATRD
QMMQASGNKLIGLFSTTSHLEYEIDRVAGKGEGATQPSLADMTAKAIELLSKNGNGYFLMVEGGRIDHALHGTNAKRALE
DTIAFDDAIKRALSMVDLSNTIIVVTADHDHTMTINGYSHRGNPILATATDYKTKKTALAADGLPYTTLVFGNGGTPRKA
TRDNPSLVDTTANDYLQEVGVNLGSPGAETHGGGDVMLFSTGPGSAPLKGTLDNTKVFGVVKSALGL

Sequences:

>Translated_467_residues
MKITTRIGMLAAAAASACLTMPAAQAAGEAKNVIFFLGDGMGPATVTASRIYKYGESGKLTMESLKRTARIKTFSNDAQT
TDSAPSMAAYMTGVKMNNEVISMSPDTKATAPGTDVNGNKTINNCLPTNGTSVPTLLELAKARGKATGAITTTELTHATP
AATYSHICHRDAQYDIAAQAVPGGAGFNTALGDGVDVLMGGGRNHWTPFDATANKKGRADGRNLLTEFAAKGYAVAATRD
QMMQASGNKLIGLFSTTSHLEYEIDRVAGKGEGATQPSLADMTAKAIELLSKNGNGYFLMVEGGRIDHALHGTNAKRALE
DTIAFDDAIKRALSMVDLSNTIIVVTADHDHTMTINGYSHRGNPILATATDYKTKKTALAADGLPYTTLVFGNGGTPRKA
TRDNPSLVDTTANDYLQEVGVNLGSPGAETHGGGDVMLFSTGPGSAPLKGTLDNTKVFGVVKSALGL
>Mature_467_residues
MKITTRIGMLAAAAASACLTMPAAQAAGEAKNVIFFLGDGMGPATVTASRIYKYGESGKLTMESLKRTARIKTFSNDAQT
TDSAPSMAAYMTGVKMNNEVISMSPDTKATAPGTDVNGNKTINNCLPTNGTSVPTLLELAKARGKATGAITTTELTHATP
AATYSHICHRDAQYDIAAQAVPGGAGFNTALGDGVDVLMGGGRNHWTPFDATANKKGRADGRNLLTEFAAKGYAVAATRD
QMMQASGNKLIGLFSTTSHLEYEIDRVAGKGEGATQPSLADMTAKAIELLSKNGNGYFLMVEGGRIDHALHGTNAKRALE
DTIAFDDAIKRALSMVDLSNTIIVVTADHDHTMTINGYSHRGNPILATATDYKTKKTALAADGLPYTTLVFGNGGTPRKA
TRDNPSLVDTTANDYLQEVGVNLGSPGAETHGGGDVMLFSTGPGSAPLKGTLDNTKVFGVVKSALGL

Specific function: Specifically cleaves both streptomycin-6-phosphate and, more slowly, streptomycin-3''-phosphate during the biosynthesis of streptomycin [H]

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266292, Length=465, Percent_Identity=36.1290322580645, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI94721246, Length=486, Percent_Identity=36.4197530864198, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI157266296, Length=486, Percent_Identity=35.8024691358025, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI116734717, Length=466, Percent_Identity=35.4077253218884, Blast_Score=231, Evalue=8e-61,
Organism=Homo sapiens, GI294660770, Length=461, Percent_Identity=34.70715835141, Blast_Score=222, Evalue=6e-58,
Organism=Homo sapiens, GI294660772, Length=417, Percent_Identity=33.5731414868106, Blast_Score=194, Evalue=2e-49,
Organism=Escherichia coli, GI48994877, Length=476, Percent_Identity=31.0924369747899, Blast_Score=146, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6320689, Length=368, Percent_Identity=31.5217391304348, Blast_Score=140, Evalue=4e-34,
Organism=Drosophila melanogaster, GI21355149, Length=454, Percent_Identity=35.6828193832599, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI21358067, Length=475, Percent_Identity=34.9473684210526, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI85725200, Length=460, Percent_Identity=33.695652173913, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI85815833, Length=460, Percent_Identity=33.695652173913, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI21355151, Length=460, Percent_Identity=34.5652173913044, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24585213, Length=473, Percent_Identity=33.1923890063425, Blast_Score=221, Evalue=1e-57,
Organism=Drosophila melanogaster, GI21355981, Length=452, Percent_Identity=34.070796460177, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI19921912, Length=471, Percent_Identity=33.5456475583864, Blast_Score=213, Evalue=3e-55,
Organism=Drosophila melanogaster, GI24651554, Length=498, Percent_Identity=32.7309236947791, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI18859923, Length=447, Percent_Identity=33.1096196868009, Blast_Score=201, Evalue=9e-52,
Organism=Drosophila melanogaster, GI24651556, Length=486, Percent_Identity=32.0987654320988, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24657827, Length=460, Percent_Identity=33.9130434782609, Blast_Score=189, Evalue=3e-48,
Organism=Drosophila melanogaster, GI24657842, Length=484, Percent_Identity=31.198347107438, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24657835, Length=465, Percent_Identity=31.1827956989247, Blast_Score=179, Evalue=5e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.39 [H]

Molecular weight: Translated: 48616; Mature: 48616

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITTRIGMLAAAAASACLTMPAAQAAGEAKNVIFFLGDGMGPATVTASRIYKYGESGKL
CEEEHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCE
TMESLKRTARIKTFSNDAQTTDSAPSMAAYMTGVKMNNEVISMSPDTKATAPGTDVNGNK
EHHHHHHHHHEEEECCCCCCCCCCCCHHHHHCCEEECCEEEEECCCCCCCCCCCCCCCCC
TINNCLPTNGTSVPTLLELAKARGKATGAITTTELTHATPAATYSHICHRDAQYDIAAQA
EECCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEEE
VPGGAGFNTALGDGVDVLMGGGRNHWTPFDATANKKGRADGRNLLTEFAAKGYAVAATRD
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECHH
QMMQASGNKLIGLFSTTSHLEYEIDRVAGKGEGATQPSLADMTAKAIELLSKNGNGYFLM
HHHHCCCCEEEEEEECCCCCEEEEEHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEE
VEGGRIDHALHGTNAKRALEDTIAFDDAIKRALSMVDLSNTIIVVTADHDHTMTINGYSH
EECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCC
RGNPILATATDYKTKKTALAADGLPYTTLVFGNGGTPRKATRDNPSLVDTTANDYLQEVG
CCCEEEEEECCCCCCHHEEECCCCCEEEEEECCCCCCCCCCCCCCCEEECCHHHHHHHHC
VNLGSPGAETHGGGDVMLFSTGPGSAPLKGTLDNTKVFGVVKSALGL
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKITTRIGMLAAAAASACLTMPAAQAAGEAKNVIFFLGDGMGPATVTASRIYKYGESGKL
CEEEHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCE
TMESLKRTARIKTFSNDAQTTDSAPSMAAYMTGVKMNNEVISMSPDTKATAPGTDVNGNK
EHHHHHHHHHEEEECCCCCCCCCCCCHHHHHCCEEECCEEEEECCCCCCCCCCCCCCCCC
TINNCLPTNGTSVPTLLELAKARGKATGAITTTELTHATPAATYSHICHRDAQYDIAAQA
EECCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEEE
VPGGAGFNTALGDGVDVLMGGGRNHWTPFDATANKKGRADGRNLLTEFAAKGYAVAATRD
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECHH
QMMQASGNKLIGLFSTTSHLEYEIDRVAGKGEGATQPSLADMTAKAIELLSKNGNGYFLM
HHHHCCCCEEEEEEECCCCCEEEEEHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEE
VEGGRIDHALHGTNAKRALEDTIAFDDAIKRALSMVDLSNTIIVVTADHDHTMTINGYSH
EECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCC
RGNPILATATDYKTKKTALAADGLPYTTLVFGNGGTPRKATRDNPSLVDTTANDYLQEVG
CCCEEEEEECCCCCCHHEEECCCCCEEEEEECCCCCCCCCCCCCCCEEECCHHHHHHHHC
VNLGSPGAETHGGGDVMLFSTGPGSAPLKGTLDNTKVFGVVKSALGL
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1654502 [H]