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The map label for this gene is frzE [H]

Identifier: 94312897

GI number: 94312897

Start: 531721

End: 534027

Strand: Reverse

Name: frzE [H]

Synonym: Rmet_3969

Alternate gene names: 94312897

Gene position: 534027-531721 (Counterclockwise)

Preceding gene: 94312898

Following gene: 94312896

Centisome position: 20.7

GC content: 66.93

Gene sequence:

>2307_bases
ATGAATCCGGAACAGCTCCGTGATGCCTCGTTGCTGGACCTGTTCGCGCTCGAAGCCGACGCGCAGGCCGATGTGCTCAA
TGCCGGCCTGCTGGCCCTGGAGCGCGATCCCACGGCAGCCGTCCAGCTCGAGGCATGCATGCGCGCCGCGCATTCGCTAA
AAGGGGCCGCGCGCATCGTCGGGCTCGAAGGTGGCGTGCGCGTTGCGCATGTGATGGAGGATTGCCTCGTCGCCGCGCAA
CGTGGCGGCCTTCTGCTGAGCGCCTCCCATATCGATGCACTGCTGCAAGGCACTGACCTGCTGCAGCGAATCGGCCATCC
GCCCGATGGCAACGCCAATTGGCCGGAGCTTGGCGGCAAGGCAGAGATCGACGCTTTCGTCGCCCGTCTCGAGGCGCTGC
TCGATGGCGGCGCGGATGTGGCGGCACTGGCAGCACCGACAGTGTTGCCGCCGGCCGCCGAGCCCGCGCCGCCGGAGCCC
CCGTTACCCACGGTCAATCACCCGTCAACGAGCACCGTCGTCCCGGCGCAGCCACAGGAGTACACCGGCAGCAACCAGGA
ACGCATCCTGCGCGTCAATGCCGAACGGCTGGACCGGCTGCTGGCGATGGCCGGCGAGTCGATGGTGGAGACGCATTGGC
TACGGCCGTTCGGAGTTGCGATCCAGCAGGCCAGGCGTCAACAGATGCGCGTGATGCAGGCGCTCGATGGCGTTCAGGCG
CTGCTCGACAGCCCCGATAGCACCGTGCCACGCCTGCGGGCGGCACTGATGGAGACTCGCCAACTGCTTGAGGACAGCCA
CGGCCAACTCACCCAGCGCGTGGAGGAATTCGACCAGTACGATCACCGCCAAAGTGGACTGTCGCGCCGCCTGTACGACA
CGGCGCTGTCGTGCCGGATGCGCCCACTGGCCGATGGCATCACCGGCTACTCGCGCATGGTGCGCGACCTGGGCCGCTCG
CTCGGCAAGCGTGTCCGACTGGAGTTGTCCGGCGAGCAAACCCAGGTCGACCGGGACATCCTGGAGCATCTCGACGCGCC
GCTGGCTCACCTGCTGCGCAATGCCGTGGATCATGGCATCGAAGCGCCGGACACGCGCCGCGCCGCCGGCAAGCCGGAAG
AAGGCCGCGTATCACTCCATGCGTGGCACAACGCCGGCCGGCTTGTGATCGAGATCTTCGATGACGGCGCGGGCGTCGAT
CTGGAAGCGTTGCGCGCCGCCATCGTGCGGCGAGGCCTCGCCCAGGCGGACACCGTCGCGCGGCTATCGCAAGCCGAATT
GCTCGAGTTCCTGCTGCTGCCGGGTTTCAGCATGCGCGAGACCGTTTCCGAGGTCTCCGGCCGCGGTGTCGGGCTCGACG
CCGTGCAGGACATGGTGCGTGCCGTGCGGGGCAGCCTGAGGCTTTCCCAGCAGCCTGGCAGCGGCCTGCACTTCCATCTC
GAGTTGCCCCTGACGCTGTCGATCGTGCGGGCGCTGCTCGTGGAAATATCGGGCGAAGCCTATGCCTTCCCGCTGGGCCA
CGTGCTGCGTGCCGTGGCCGTGCGCCGCGAAGACATCGAACAGACCGAGCAGCACCAGCACTTCCGCCACGATGGCAAGC
CGGTGGGGCTGATCAGCGCCGCGCAGGTGCTCCAGCGTCCCGAGCGTGGCCAGGATGGAGATACCGTTCCCGTGGTCGTC
ATCGGCGAGCAGGATCGTGTGTATGGCATCGCAGTGGATCGCCTGATCGGCGAGCGATTGCTGGTGGTGCAGCCACTCGC
GCAGGCCCTCGGCAAGATCAAGGACATCGCGGCGGGCAGCCTGACCGACGATGGCATGCCGGTCCTGATCTTCGACGTCG
AGGACATGCTGCGGTCGGTGGAGAAACTCGTCTCCGATGGCCGCATCGAACGGGTGCGCCACGGCGGCGAGGAAGCCACC
CAGACGCGACGCAAGCGCGTGCTGGTGGTGGATGACTCGCTGACCGTGCGGGAACTGGAGCGCAAGCTGCTGAGCGGACG
CGGCTACGACGTGGCCGTCGCGGTGGACGGCATGGATGGATGGAACGTGCTCCGCGCGGAATCGTTCGATCTGGTGATCA
CCGACGTCGACATGCCACGCATGGACGGCATTGAACTCGTCAGCCGGATACGAGGCGATACCCGCCTGACGCATCTCCCC
GTGATGATCGTCTCTTACAAGGACCGCGAGCAGGATCGCGAGCGCGGCCTGCAGGCTGGCGCGGATTACTATCTGGCCAA
AGGCAGCTTCCACGATACCGCGCTGCTCGACGCGGTACGTGACCTGATCGGCGAGGCACGCTCATGA

Upstream 100 bases:

>100_bases
CCTCCGCCCGCTATTCACGCGCGCTGTTCGATTTCAACGGCCACAGCGTCGGTCTGCTTGATGCCGACCTGCTGCGGCAG
GCGTTGACCCGGAGCCTGTC

Downstream 100 bases:

>100_bases
GAATCGGCATCGTCAATGATTCCGCACTGGCTGTTGCCGCGTTGCGGCGCGCGCTTGCGCTGGACACCACGCTCGAAATC
GCCTGGATCGCCGGCGACGG

Product: signal transduction histidine kinase (cheA)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 768; Mature: 768

Protein sequence:

>768_residues
MNPEQLRDASLLDLFALEADAQADVLNAGLLALERDPTAAVQLEACMRAAHSLKGAARIVGLEGGVRVAHVMEDCLVAAQ
RGGLLLSASHIDALLQGTDLLQRIGHPPDGNANWPELGGKAEIDAFVARLEALLDGGADVAALAAPTVLPPAAEPAPPEP
PLPTVNHPSTSTVVPAQPQEYTGSNQERILRVNAERLDRLLAMAGESMVETHWLRPFGVAIQQARRQQMRVMQALDGVQA
LLDSPDSTVPRLRAALMETRQLLEDSHGQLTQRVEEFDQYDHRQSGLSRRLYDTALSCRMRPLADGITGYSRMVRDLGRS
LGKRVRLELSGEQTQVDRDILEHLDAPLAHLLRNAVDHGIEAPDTRRAAGKPEEGRVSLHAWHNAGRLVIEIFDDGAGVD
LEALRAAIVRRGLAQADTVARLSQAELLEFLLLPGFSMRETVSEVSGRGVGLDAVQDMVRAVRGSLRLSQQPGSGLHFHL
ELPLTLSIVRALLVEISGEAYAFPLGHVLRAVAVRREDIEQTEQHQHFRHDGKPVGLISAAQVLQRPERGQDGDTVPVVV
IGEQDRVYGIAVDRLIGERLLVVQPLAQALGKIKDIAAGSLTDDGMPVLIFDVEDMLRSVEKLVSDGRIERVRHGGEEAT
QTRRKRVLVVDDSLTVRELERKLLSGRGYDVAVAVDGMDGWNVLRAESFDLVITDVDMPRMDGIELVSRIRGDTRLTHLP
VMIVSYKDREQDRERGLQAGADYYLAKGSFHDTALLDAVRDLIGEARS

Sequences:

>Translated_768_residues
MNPEQLRDASLLDLFALEADAQADVLNAGLLALERDPTAAVQLEACMRAAHSLKGAARIVGLEGGVRVAHVMEDCLVAAQ
RGGLLLSASHIDALLQGTDLLQRIGHPPDGNANWPELGGKAEIDAFVARLEALLDGGADVAALAAPTVLPPAAEPAPPEP
PLPTVNHPSTSTVVPAQPQEYTGSNQERILRVNAERLDRLLAMAGESMVETHWLRPFGVAIQQARRQQMRVMQALDGVQA
LLDSPDSTVPRLRAALMETRQLLEDSHGQLTQRVEEFDQYDHRQSGLSRRLYDTALSCRMRPLADGITGYSRMVRDLGRS
LGKRVRLELSGEQTQVDRDILEHLDAPLAHLLRNAVDHGIEAPDTRRAAGKPEEGRVSLHAWHNAGRLVIEIFDDGAGVD
LEALRAAIVRRGLAQADTVARLSQAELLEFLLLPGFSMRETVSEVSGRGVGLDAVQDMVRAVRGSLRLSQQPGSGLHFHL
ELPLTLSIVRALLVEISGEAYAFPLGHVLRAVAVRREDIEQTEQHQHFRHDGKPVGLISAAQVLQRPERGQDGDTVPVVV
IGEQDRVYGIAVDRLIGERLLVVQPLAQALGKIKDIAAGSLTDDGMPVLIFDVEDMLRSVEKLVSDGRIERVRHGGEEAT
QTRRKRVLVVDDSLTVRELERKLLSGRGYDVAVAVDGMDGWNVLRAESFDLVITDVDMPRMDGIELVSRIRGDTRLTHLP
VMIVSYKDREQDRERGLQAGADYYLAKGSFHDTALLDAVRDLIGEARS
>Mature_768_residues
MNPEQLRDASLLDLFALEADAQADVLNAGLLALERDPTAAVQLEACMRAAHSLKGAARIVGLEGGVRVAHVMEDCLVAAQ
RGGLLLSASHIDALLQGTDLLQRIGHPPDGNANWPELGGKAEIDAFVARLEALLDGGADVAALAAPTVLPPAAEPAPPEP
PLPTVNHPSTSTVVPAQPQEYTGSNQERILRVNAERLDRLLAMAGESMVETHWLRPFGVAIQQARRQQMRVMQALDGVQA
LLDSPDSTVPRLRAALMETRQLLEDSHGQLTQRVEEFDQYDHRQSGLSRRLYDTALSCRMRPLADGITGYSRMVRDLGRS
LGKRVRLELSGEQTQVDRDILEHLDAPLAHLLRNAVDHGIEAPDTRRAAGKPEEGRVSLHAWHNAGRLVIEIFDDGAGVD
LEALRAAIVRRGLAQADTVARLSQAELLEFLLLPGFSMRETVSEVSGRGVGLDAVQDMVRAVRGSLRLSQQPGSGLHFHL
ELPLTLSIVRALLVEISGEAYAFPLGHVLRAVAVRREDIEQTEQHQHFRHDGKPVGLISAAQVLQRPERGQDGDTVPVVV
IGEQDRVYGIAVDRLIGERLLVVQPLAQALGKIKDIAAGSLTDDGMPVLIFDVEDMLRSVEKLVSDGRIERVRHGGEEAT
QTRRKRVLVVDDSLTVRELERKLLSGRGYDVAVAVDGMDGWNVLRAESFDLVITDVDMPRMDGIELVSRIRGDTRLTHLP
VMIVSYKDREQDRERGLQAGADYYLAKGSFHDTALLDAVRDLIGEARS

Specific function: FrzE is involved in a sensory transduction pathway that controls the frequency at which cells reverse their gliding direction. FrzE seems to be capable of autophosphorylating itself on an histidine residue and then to transfer that group to an aspartate r

COG id: COG0643

COG function: function code NT; Chemotaxis protein histidine kinase and related kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788197, Length=359, Percent_Identity=33.4261838440111, Blast_Score=192, Evalue=6e-50,
Organism=Escherichia coli, GI1788191, Length=103, Percent_Identity=40.7766990291262, Blast_Score=76, Evalue=8e-15,
Organism=Escherichia coli, GI145693157, Length=101, Percent_Identity=41.5841584158416, Blast_Score=68, Evalue=3e-12,
Organism=Escherichia coli, GI1788713, Length=108, Percent_Identity=35.1851851851852, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI1789809, Length=118, Percent_Identity=32.2033898305085, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR002545
- InterPro:   IPR011006
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR005467
- InterPro:   IPR001789 [H]

Pfam domain/function: PF01584 CheW; PF02518 HATPase_c; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 83963; Mature: 83963

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS50851 CHEW ; PS50894 HPT ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPEQLRDASLLDLFALEADAQADVLNAGLLALERDPTAAVQLEACMRAAHSLKGAARIV
CCHHHHCCHHHHHHHHHCCCCHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEE
GLEGGVRVAHVMEDCLVAAQRGGLLLSASHIDALLQGTDLLQRIGHPPDGNANWPELGGK
ECCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
AEIDAFVARLEALLDGGADVAALAAPTVLPPAAEPAPPEPPLPTVNHPSTSTVVPAQPQE
HHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
YTGSNQERILRVNAERLDRLLAMAGESMVETHWLRPFGVAIQQARRQQMRVMQALDGVQA
CCCCCCHHEEEECHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLDSPDSTVPRLRAALMETRQLLEDSHGQLTQRVEEFDQYDHRQSGLSRRLYDTALSCRM
HHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RPLADGITGYSRMVRDLGRSLGKRVRLELSGEQTQVDRDILEHLDAPLAHLLRNAVDHGI
HHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHCCC
EAPDTRRAAGKPEEGRVSLHAWHNAGRLVIEIFDDGAGVDLEALRAAIVRRGLAQADTVA
CCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
RLSQAELLEFLLLPGFSMRETVSEVSGRGVGLDAVQDMVRAVRGSLRLSQQPGSGLHFHL
HHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEECCCCCCCEEEEE
ELPLTLSIVRALLVEISGEAYAFPLGHVLRAVAVRREDIEQTEQHQHFRHDGKPVGLISA
ECCHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
AQVLQRPERGQDGDTVPVVVIGEQDRVYGIAVDRLIGERLLVVQPLAQALGKIKDIAAGS
HHHHHCCCCCCCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LTDDGMPVLIFDVEDMLRSVEKLVSDGRIERVRHGGEEATQTRRKRVLVVDDSLTVRELE
CCCCCCCEEEECHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHCEEEEECCCCHHHHHH
RKLLSGRGYDVAVAVDGMDGWNVLRAESFDLVITDVDMPRMDGIELVSRIRGDTRLTHLP
HHHHCCCCCEEEEEEECCCCCCEEEECCCEEEEEECCCCCCCHHHHHHHHCCCCEEEECC
VMIVSYKDREQDRERGLQAGADYYLAKGSFHDTALLDAVRDLIGEARS
EEEEECCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNPEQLRDASLLDLFALEADAQADVLNAGLLALERDPTAAVQLEACMRAAHSLKGAARIV
CCHHHHCCHHHHHHHHHCCCCHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEE
GLEGGVRVAHVMEDCLVAAQRGGLLLSASHIDALLQGTDLLQRIGHPPDGNANWPELGGK
ECCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
AEIDAFVARLEALLDGGADVAALAAPTVLPPAAEPAPPEPPLPTVNHPSTSTVVPAQPQE
HHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
YTGSNQERILRVNAERLDRLLAMAGESMVETHWLRPFGVAIQQARRQQMRVMQALDGVQA
CCCCCCHHEEEECHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLDSPDSTVPRLRAALMETRQLLEDSHGQLTQRVEEFDQYDHRQSGLSRRLYDTALSCRM
HHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RPLADGITGYSRMVRDLGRSLGKRVRLELSGEQTQVDRDILEHLDAPLAHLLRNAVDHGI
HHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHCCC
EAPDTRRAAGKPEEGRVSLHAWHNAGRLVIEIFDDGAGVDLEALRAAIVRRGLAQADTVA
CCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
RLSQAELLEFLLLPGFSMRETVSEVSGRGVGLDAVQDMVRAVRGSLRLSQQPGSGLHFHL
HHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEECCCCCCCEEEEE
ELPLTLSIVRALLVEISGEAYAFPLGHVLRAVAVRREDIEQTEQHQHFRHDGKPVGLISA
ECCHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
AQVLQRPERGQDGDTVPVVVIGEQDRVYGIAVDRLIGERLLVVQPLAQALGKIKDIAAGS
HHHHHCCCCCCCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LTDDGMPVLIFDVEDMLRSVEKLVSDGRIERVRHGGEEATQTRRKRVLVVDDSLTVRELE
CCCCCCCEEEECHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHCEEEEECCCCHHHHHH
RKLLSGRGYDVAVAVDGMDGWNVLRAESFDLVITDVDMPRMDGIELVSRIRGDTRLTHLP
HHHHCCCCCEEEEEEECCCCCCEEEECCCEEEEEECCCCCCCHHHHHHHHCCCCEEEECC
VMIVSYKDREQDRERGLQAGADYYLAKGSFHDTALLDAVRDLIGEARS
EEEEECCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2165608; 2123853 [H]