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The map label for this gene is cheB2

Identifier: 94312896

GI number: 94312896

Start: 530708

End: 531724

Strand: Reverse

Name: cheB2

Synonym: Rmet_3968

Alternate gene names: 94312896

Gene position: 531724-530708 (Counterclockwise)

Preceding gene: 94312897

Following gene: 94312895

Centisome position: 20.61

GC content: 67.35

Gene sequence:

>1017_bases
ATGAGAATCGGCATCGTCAATGATTCCGCACTGGCTGTTGCCGCGTTGCGGCGCGCGCTTGCGCTGGACACCACGCTCGA
AATCGCCTGGATCGCCGGCGACGGCGAGGAAGCCGTGCGGATGGCCGCGACCCAGACCCCCGACCTGATCCTGATGGACC
TGCTGATGCCGGTCATGGACGGCGTGGAAGCCACACGGCGCATCATGGCCGAATCGCCGTGCGCGATCGTCGTGGTGACC
ATGGACCTCGGTCGTAACGCGAACCAGGTGTTTGATGCCATGGGGCACGGTGCGATCGATGCCGTGGACACCCCGACGCT
CACCGACTCTGACACCAAGCTCGCGGCCGGCCCGCTATTGCGAAAGATCCGAAACATCGCACGCCTGCTTGGCGGACGCG
GGCAGGCGCCCCATCCGCTCGCGGCGGCCACGCCAACGCCCACCGCACCCCGGCTGGTCGCGATTGGCGCATCGGCCGGC
GGCCCGGCCGCGCTGGCCACGCTGCTCGGCGCGTTGCCAGCCGACTTTGGCGCGGCGGTCGTCGCCGTGCAACATGTCGA
CGAAGCGTTCGCCCAGGGCATGGCCGAGTGGCTCGACGCGCAGTGCCAACTACCAGTGCGCCTTGCCCGGGCTGGAGAGG
TACCGCAGGCTGGCGCGGTGGTACTGGCCGGCACGAACGATCACTTGCGCCTGACCAGCGCGGGACGCCTGATCTACACC
CCCGACCCTTGCGACTACCTGTATCGCCCTTCGATCGATGTGTTTTTTGAAAGCGTGGTGGAACACTGGCGCGGTGAAGC
AATCGGAGTATTGCTGACCGGCATGGGACGCGATGGGGCGCAAGGCCTGAAGGCCATGCGTGAGCGCGGCTTCCAGACCA
TCGCGCAGGATCAGGCCACGAGCGCCGTCTACGGGATGCCCAAGGCGGCGGCCACGCTGGGCGCGGCGAGCGAGATTCTG
CCACTGCAGAAGATCGCGCCGCGGCTAGTCATGACGTGTGGTGGTGGTCGGCGGTAA

Upstream 100 bases:

>100_bases
GCGGCCTGCAGGCTGGCGCGGATTACTATCTGGCCAAAGGCAGCTTCCACGATACCGCGCTGCTCGACGCGGTACGTGAC
CTGATCGGCGAGGCACGCTC

Downstream 100 bases:

>100_bases
AAGAAAAGAACAGGAGGCGAATAATGCCCAGACAACTCAATTCGGCCGATGCGGCGGCCACCCATGAATACCCGGCAATG
GTGCTGCTGGTCGACGACCA

Product: chemotaxis-specific methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MRIGIVNDSALAVAALRRALALDTTLEIAWIAGDGEEAVRMAATQTPDLILMDLLMPVMDGVEATRRIMAESPCAIVVVT
MDLGRNANQVFDAMGHGAIDAVDTPTLTDSDTKLAAGPLLRKIRNIARLLGGRGQAPHPLAAATPTPTAPRLVAIGASAG
GPAALATLLGALPADFGAAVVAVQHVDEAFAQGMAEWLDAQCQLPVRLARAGEVPQAGAVVLAGTNDHLRLTSAGRLIYT
PDPCDYLYRPSIDVFFESVVEHWRGEAIGVLLTGMGRDGAQGLKAMRERGFQTIAQDQATSAVYGMPKAAATLGAASEIL
PLQKIAPRLVMTCGGGRR

Sequences:

>Translated_338_residues
MRIGIVNDSALAVAALRRALALDTTLEIAWIAGDGEEAVRMAATQTPDLILMDLLMPVMDGVEATRRIMAESPCAIVVVT
MDLGRNANQVFDAMGHGAIDAVDTPTLTDSDTKLAAGPLLRKIRNIARLLGGRGQAPHPLAAATPTPTAPRLVAIGASAG
GPAALATLLGALPADFGAAVVAVQHVDEAFAQGMAEWLDAQCQLPVRLARAGEVPQAGAVVLAGTNDHLRLTSAGRLIYT
PDPCDYLYRPSIDVFFESVVEHWRGEAIGVLLTGMGRDGAQGLKAMRERGFQTIAQDQATSAVYGMPKAAATLGAASEIL
PLQKIAPRLVMTCGGGRR
>Mature_338_residues
MRIGIVNDSALAVAALRRALALDTTLEIAWIAGDGEEAVRMAATQTPDLILMDLLMPVMDGVEATRRIMAESPCAIVVVT
MDLGRNANQVFDAMGHGAIDAVDTPTLTDSDTKLAAGPLLRKIRNIARLLGGRGQAPHPLAAATPTPTAPRLVAIGASAG
GPAALATLLGALPADFGAAVVAVQHVDEAFAQGMAEWLDAQCQLPVRLARAGEVPQAGAVVLAGTNDHLRLTSAGRLIYT
PDPCDYLYRPSIDVFFESVVEHWRGEAIGVLLTGMGRDGAQGLKAMRERGFQTIAQDQATSAVYGMPKAAATLGAASEIL
PLQKIAPRLVMTCGGGRR

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=346, Percent_Identity=31.5028901734104, Blast_Score=161, Evalue=6e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB2_RALME (Q1LG90)

Other databases:

- EMBL:   CP000353
- RefSeq:   YP_586105.1
- ProteinModelPortal:   Q1LG90
- SMR:   Q1LG90
- STRING:   Q1LG90
- GeneID:   4040826
- GenomeReviews:   CP000353_GR
- KEGG:   rme:Rmet_3968
- eggNOG:   COG2201
- HOGENOM:   HBG705324
- OMA:   FFESVVE
- ProtClustDB:   PRK12555
- BioCyc:   RMET266264:RMET_3968-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 35253; Mature: 35253

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 158-158 ACT_SITE 185-185 ACT_SITE 278-278

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGIVNDSALAVAALRRALALDTTLEIAWIAGDGEEAVRMAATQTPDLILMDLLMPVMD
CEEEEECCHHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHH
GVEATRRIMAESPCAIVVVTMDLGRNANQVFDAMGHGAIDAVDTPTLTDSDTKLAAGPLL
HHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
RKIRNIARLLGGRGQAPHPLAAATPTPTAPRLVAIGASAGGPAALATLLGALPADFGAAV
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHH
VAVQHVDEAFAQGMAEWLDAQCQLPVRLARAGEVPQAGAVVLAGTNDHLRLTSAGRLIYT
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEEEECCCCCEEEEECCEEEEC
PDPCDYLYRPSIDVFFESVVEHWRGEAIGVLLTGMGRDGAQGLKAMRERGFQTIAQDQAT
CCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHH
SAVYGMPKAAATLGAASEILPLQKIAPRLVMTCGGGRR
HHHHCCCHHHHHHCCHHHHCCHHHHCCHHEEECCCCCC
>Mature Secondary Structure
MRIGIVNDSALAVAALRRALALDTTLEIAWIAGDGEEAVRMAATQTPDLILMDLLMPVMD
CEEEEECCHHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHH
GVEATRRIMAESPCAIVVVTMDLGRNANQVFDAMGHGAIDAVDTPTLTDSDTKLAAGPLL
HHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
RKIRNIARLLGGRGQAPHPLAAATPTPTAPRLVAIGASAGGPAALATLLGALPADFGAAV
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHH
VAVQHVDEAFAQGMAEWLDAQCQLPVRLARAGEVPQAGAVVLAGTNDHLRLTSAGRLIYT
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEEEECCCCCEEEEECCEEEEC
PDPCDYLYRPSIDVFFESVVEHWRGEAIGVLLTGMGRDGAQGLKAMRERGFQTIAQDQAT
CCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHH
SAVYGMPKAAATLGAASEILPLQKIAPRLVMTCGGGRR
HHHHCCCHHHHHHCCHHHHCCHHHHCCHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA