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The map label for this gene is acrA [H]

Identifier: 94312851

GI number: 94312851

Start: 479175

End: 480338

Strand: Direct

Name: acrA [H]

Synonym: Rmet_3923

Alternate gene names: 94312851

Gene position: 479175-480338 (Clockwise)

Preceding gene: 291481499

Following gene: 94312852

Centisome position: 18.57

GC content: 62.8

Gene sequence:

>1164_bases
ATGCAAGCAATGCGCGCCCGGCGTGCTTTGATTCCAGGCATTCTGGCAGTTGCCGTAACAGTACTGTCTGCGTGCGGCAA
GGACAACAAGCCGCCTGCGGCCCAGACGCCTGAAGTGAAAGTTGTCACGCTGAAGGACGAGAACGTTACGCTCGACAGTT
CCTTGCCTGGGCGCACCATGCCCTACCGGATCGCCGAGGTTCGCCCTCAGGTCAATGGCATCCTGCAGAAGCGTCTGTTT
GTCGAAGGCAGCGATGTGAAGCAGGGCCAGCAGTTGTACCAGATCGATCCGTCGACCTATGAAGCTACTGCGAAGAGCGC
CGAGGCAAATCTTGAATCGGCGCGCTTGCTTGCCGAACGCTACGGCAAGCTGGTCGGAGAGGAAGCCGTCAGCAAGCAGC
AGTACGCCGAGGCGCAGGCATCCTACCTGCAAGCTAAAGCCGCTGTGGACCAAGCGCATATCAATCTGCGCTACACCAAG
GTCCTTTCGCCGATTTCCGGCCGTATCAGCCGATCGCTCGTCACCGAAGGCGCGCTGGTAACGAACGGCCAGGCTAACGC
GCTGGCAACGATTCAGCAGCTCGATCCGATCTATGTGGATGTGACGCAGCCGTCGTCGGCAATCCTCCGCCTGCGTCGTG
AACTGGCCAACGGCCAACTGCAGGGTGCCGGTGCGAATGCGGCCAAGGTTACGCTGCAGCTCGAAGATGGCAGCATCTAT
TCGGAGCCCGGTCGTCTCGAGTTCTCGGAAGTCGCGGTCGACCAGGGCACGGGTTCCGTGACGCTGCGCGCCGTCTTCCC
GAACCCGCGCAATGAACTGCTGCCGGGCATGTTCGTTCATGCGCGTCTGGCGGAAGGCGTCAAGACTGCCGCCATCCTGG
CACCGCAGCGCGGCGTCACGCGTGACCTGAAAGGACAGGCGACTTCGCTGGTGGTCAATGCCAACAACGAAGTGGAACAG
CGCATTCTCTCGACCGACCGCACGGTTGGCGACAAGTGGCTCGTTACCGCGGGCCTGAAGGCCGGGGATCGCGTGATCGT
CGAGGGTCTGCAGTTTGTGCGCCCGGGTGTGAAGGTCAAGGCCGCCGAAGCCGATGCGGCCACAGCCGGCCCCGTGGAGG
CCTCGGCACCGAGCGCGGCGTCGGCCCCCGCGGCAAAGTCCTGA

Upstream 100 bases:

>100_bases
TGGCAGGCACGCCAATTGCGTCCGCGCGTAGCGCGGTCAGCCGGTCTGGCCAACCAGGCCCCAAGAAGAACTCATCCGTT
TCATCCTCGAGGATATCGCT

Downstream 100 bases:

>100_bases
ACGGGACGGACGCATGAGGATTCGCCATGTCTAATTTCTTTATCGAGCGGCCGATCTTTGCCTGGGTGATCGCGCTGGTC
ATCATGCTGGCCGGCGTGCT

Product: multidrug efflux system

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MQAMRARRALIPGILAVAVTVLSACGKDNKPPAAQTPEVKVVTLKDENVTLDSSLPGRTMPYRIAEVRPQVNGILQKRLF
VEGSDVKQGQQLYQIDPSTYEATAKSAEANLESARLLAERYGKLVGEEAVSKQQYAEAQASYLQAKAAVDQAHINLRYTK
VLSPISGRISRSLVTEGALVTNGQANALATIQQLDPIYVDVTQPSSAILRLRRELANGQLQGAGANAAKVTLQLEDGSIY
SEPGRLEFSEVAVDQGTGSVTLRAVFPNPRNELLPGMFVHARLAEGVKTAAILAPQRGVTRDLKGQATSLVVNANNEVEQ
RILSTDRTVGDKWLVTAGLKAGDRVIVEGLQFVRPGVKVKAAEADAATAGPVEASAPSAASAPAAKS

Sequences:

>Translated_387_residues
MQAMRARRALIPGILAVAVTVLSACGKDNKPPAAQTPEVKVVTLKDENVTLDSSLPGRTMPYRIAEVRPQVNGILQKRLF
VEGSDVKQGQQLYQIDPSTYEATAKSAEANLESARLLAERYGKLVGEEAVSKQQYAEAQASYLQAKAAVDQAHINLRYTK
VLSPISGRISRSLVTEGALVTNGQANALATIQQLDPIYVDVTQPSSAILRLRRELANGQLQGAGANAAKVTLQLEDGSIY
SEPGRLEFSEVAVDQGTGSVTLRAVFPNPRNELLPGMFVHARLAEGVKTAAILAPQRGVTRDLKGQATSLVVNANNEVEQ
RILSTDRTVGDKWLVTAGLKAGDRVIVEGLQFVRPGVKVKAAEADAATAGPVEASAPSAASAPAAKS
>Mature_387_residues
MQAMRARRALIPGILAVAVTVLSACGKDNKPPAAQTPEVKVVTLKDENVTLDSSLPGRTMPYRIAEVRPQVNGILQKRLF
VEGSDVKQGQQLYQIDPSTYEATAKSAEANLESARLLAERYGKLVGEEAVSKQQYAEAQASYLQAKAAVDQAHINLRYTK
VLSPISGRISRSLVTEGALVTNGQANALATIQQLDPIYVDVTQPSSAILRLRRELANGQLQGAGANAAKVTLQLEDGSIY
SEPGRLEFSEVAVDQGTGSVTLRAVFPNPRNELLPGMFVHARLAEGVKTAAILAPQRGVTRDLKGQATSLVVNANNEVEQ
RILSTDRTVGDKWLVTAGLKAGDRVIVEGLQFVRPGVKVKAAEADAATAGPVEASAPSAASAPAAKS

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=383, Percent_Identity=55.3524804177546, Blast_Score=370, Evalue=1e-103,
Organism=Escherichia coli, GI1789665, Length=372, Percent_Identity=49.7311827956989, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1789929, Length=372, Percent_Identity=47.8494623655914, Blast_Score=327, Evalue=8e-91,
Organism=Escherichia coli, GI87082036, Length=316, Percent_Identity=27.2151898734177, Blast_Score=109, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41034; Mature: 41034

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAMRARRALIPGILAVAVTVLSACGKDNKPPAAQTPEVKVVTLKDENVTLDSSLPGRTM
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCCC
PYRIAEVRPQVNGILQKRLFVEGSDVKQGQQLYQIDPSTYEATAKSAEANLESARLLAER
CCHHHHHCHHHHHHHHHHHEECCCCCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHH
YGKLVGEEAVSKQQYAEAQASYLQAKAAVDQAHINLRYTKVLSPISGRISRSLVTEGALV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHCCHHHHHHHHHHHCCCEE
TNGQANALATIQQLDPIYVDVTQPSSAILRLRRELANGQLQGAGANAAKVTLQLEDGSIY
ECCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCEEECCCCCEEEEEEEEECCCEE
SEPGRLEFSEVAVDQGTGSVTLRAVFPNPRNELLPGMFVHARLAEGVKTAAILAPQRGVT
CCCCCEEHHHHEEECCCCCEEEEEECCCCHHHHCCCHHHHHHHHCCHHEEEEECCCCCCC
RDLKGQATSLVVNANNEVEQRILSTDRTVGDKWLVTAGLKAGDRVIVEGLQFVRPGVKVK
CCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEE
AAEADAATAGPVEASAPSAASAPAAKS
ECCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQAMRARRALIPGILAVAVTVLSACGKDNKPPAAQTPEVKVVTLKDENVTLDSSLPGRTM
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCCC
PYRIAEVRPQVNGILQKRLFVEGSDVKQGQQLYQIDPSTYEATAKSAEANLESARLLAER
CCHHHHHCHHHHHHHHHHHEECCCCCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHH
YGKLVGEEAVSKQQYAEAQASYLQAKAAVDQAHINLRYTKVLSPISGRISRSLVTEGALV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHCCHHHHHHHHHHHCCCEE
TNGQANALATIQQLDPIYVDVTQPSSAILRLRRELANGQLQGAGANAAKVTLQLEDGSIY
ECCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCEEECCCCCEEEEEEEEECCCEE
SEPGRLEFSEVAVDQGTGSVTLRAVFPNPRNELLPGMFVHARLAEGVKTAAILAPQRGVT
CCCCCEEHHHHEEECCCCCEEEEEECCCCHHHHCCCHHHHHHHHCCHHEEEEECCCCCCC
RDLKGQATSLVVNANNEVEQRILSTDRTVGDKWLVTAGLKAGDRVIVEGLQFVRPGVKVK
CCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEE
AAEADAATAGPVEASAPSAASAPAAKS
ECCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]