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The map label for this gene is ogt2 [H]

Identifier: 94312697

GI number: 94312697

Start: 305037

End: 305588

Strand: Reverse

Name: ogt2 [H]

Synonym: Rmet_3767

Alternate gene names: 94312697

Gene position: 305588-305037 (Counterclockwise)

Preceding gene: 94312698

Following gene: 94312696

Centisome position: 11.84

GC content: 66.49

Gene sequence:

>552_bases
ATGTTTCTCAGCCAACTGGATTCGCCGCTGGGTGCCATGCTGCTCGTGACCGATGCGCGGCGGGTCGTGCGTGCGCTCGA
CTTCGCCGACCACAAGTCACGGCTGTATCGCGGTCTGCGCGAGCACTATGGCGAGGTGGAACTGACTGAGATCCCTGCCC
CGGCCGAGATTGCCGATGCACTGGCCCGCTACTTCCATGGCGAGCTGGAGGCGCTGAAGGTGCTGCGGACTGCGACGTCT
GGTTCGGCGTTGCAGCGCCGGGTCTGGGCCGCGCTGCGCCGCATCCCTGTGGGCACGACCACGACCTACGGCAAGCTAGC
CAAGGCGCTGGGTTTCGACGACCCGCGCGCGGCCGTCGAGATCGGCGCGGCCAATGGCGCCAATCCGATCGCCATCGTCG
TGCCGTGCCACCGCGTCATTGCGAGCAATGGCGACCTCAAGGGCTATGCCTGGGGCCTGCACCGCAAGCGCTGGCTGCTG
GAGCATGAGAACGCGATGGTGGCAAAGAAGATGCAGCCCGAGACGCAGACCGCCATGCTGCCGGGGTTTTGA

Upstream 100 bases:

>100_bases
AGGTGGCGAGGCTTACCTCTTTGGCAAGTTCGGCGTGACGCGGCTGAAGTGATCCACGAACGAGCGATTCGATTCATTCA
AGAAGGAGTACACCGTGAAG

Downstream 100 bases:

>100_bases
TCTTGAGCGGAATTCATGCGAAGACTATTGCCGCCGACCTGTCACCACTCGCCTACCGCCGGGCCGTCAAGTTCCTGTCC
GAGTTGGACGAGGACTGGGC

Product: O-6-alkylguanine-DNA:cysteine-protein methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MFLSQLDSPLGAMLLVTDARRVVRALDFADHKSRLYRGLREHYGEVELTEIPAPAEIADALARYFHGELEALKVLRTATS
GSALQRRVWAALRRIPVGTTTTYGKLAKALGFDDPRAAVEIGAANGANPIAIVVPCHRVIASNGDLKGYAWGLHRKRWLL
EHENAMVAKKMQPETQTAMLPGF

Sequences:

>Translated_183_residues
MFLSQLDSPLGAMLLVTDARRVVRALDFADHKSRLYRGLREHYGEVELTEIPAPAEIADALARYFHGELEALKVLRTATS
GSALQRRVWAALRRIPVGTTTTYGKLAKALGFDDPRAAVEIGAANGANPIAIVVPCHRVIASNGDLKGYAWGLHRKRWLL
EHENAMVAKKMQPETQTAMLPGF
>Mature_183_residues
MFLSQLDSPLGAMLLVTDARRVVRALDFADHKSRLYRGLREHYGEVELTEIPAPAEIADALARYFHGELEALKVLRTATS
GSALQRRVWAALRRIPVGTTTTYGKLAKALGFDDPRAAVEIGAANGANPIAIVVPCHRVIASNGDLKGYAWGLHRKRWLL
EHENAMVAKKMQPETQTAMLPGF

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=186, Percent_Identity=30.1075268817204, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1787596, Length=166, Percent_Identity=43.3734939759036, Blast_Score=140, Evalue=5e-35,
Organism=Escherichia coli, GI1788542, Length=83, Percent_Identity=48.1927710843374, Blast_Score=88, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI115533068, Length=106, Percent_Identity=36.7924528301887, Blast_Score=77, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI115533070, Length=115, Percent_Identity=33.9130434782609, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6320001, Length=88, Percent_Identity=43.1818181818182, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 20142; Mature: 20142

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLSQLDSPLGAMLLVTDARRVVRALDFADHKSRLYRGLREHYGEVELTEIPAPAEIADA
CCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHH
LARYFHGELEALKVLRTATSGSALQRRVWAALRRIPVGTTTTYGKLAKALGFDDPRAAVE
HHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHEE
IGAANGANPIAIVVPCHRVIASNGDLKGYAWGLHRKRWLLEHENAMVAKKMQPETQTAML
ECCCCCCCCEEEEEEHHHHHHCCCCCCCEEEHHHHHHHHHHCCCHHHHHHCCCCHHCCCC
PGF
CCC
>Mature Secondary Structure
MFLSQLDSPLGAMLLVTDARRVVRALDFADHKSRLYRGLREHYGEVELTEIPAPAEIADA
CCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHH
LARYFHGELEALKVLRTATSGSALQRRVWAALRRIPVGTTTTYGKLAKALGFDDPRAAVE
HHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHEE
IGAANGANPIAIVVPCHRVIASNGDLKGYAWGLHRKRWLLEHENAMVAKKMQPETQTAML
ECCCCCCCCEEEEEEHHHHHHCCCCCCCEEEHHHHHHHHHHCCCHHHHHHCCCCHHCCCC
PGF
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]