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The map label for this gene is alkA [H]

Identifier: 94312696

GI number: 94312696

Start: 304378

End: 305034

Strand: Reverse

Name: alkA [H]

Synonym: Rmet_3766

Alternate gene names: 94312696

Gene position: 305034-304378 (Counterclockwise)

Preceding gene: 94312697

Following gene: 94312695

Centisome position: 11.82

GC content: 64.69

Gene sequence:

>657_bases
TTGAGCGGAATTCATGCGAAGACTATTGCCGCCGACCTGTCACCACTCGCCTACCGCCGGGCCGTCAAGTTCCTGTCCGA
GTTGGACGAGGACTGGGCTCTCCACATTGCCGCCATCGGACCATGTCGCCATGAGGCCAAGCCTGCACGCGAGCCCTATG
AGGCGCTGGTGCGGGCCATTGCCTATCAGCAACTGCACGCCAGGGCGGGAGATGCAATCCTGGGCCGCCTGCTGGCGCTC
TACCCCGGCGTGTCTTTTCCGGCGCCAGAGCAGTTGCTGGCCAGCGACCCTGAATTGCAGCGCGCCTGCGGCTTCTCGGC
AGAAAAGCTGGCTACCATTCGGCGGATTGCCCAGGCTACAGTCGAAGGCCTTGTGCCGAACCTCGAAGAGGCCCGGCGTC
TGCCTGACACTGCGCTGATCGAACGCCTGACGTCCCTGCGAGGTGTCGGGCGCTGGACGGTCGAAATGTTCCTGATCTAT
TCGCTGGAGCGATCCGACGTCCTGCCAGTGGACGACTTCGGCGTGCGGGAGGGTTATGGACGCCTGAAGGGTCTGGAGAG
GGCCCCCACGCCGCGTCAGATGCGCGAGATCGGAGAGGCCTGGAGCCCTTTCCGGACGGTGGCCGCCTGGTATCTGTGGC
GCCTGCCCAGTCGATAG

Upstream 100 bases:

>100_bases
GCCTGCACCGCAAGCGCTGGCTGCTGGAGCATGAGAACGCGATGGTGGCAAAGAAGATGCAGCCCGAGACGCAGACCGCC
ATGCTGCCGGGGTTTTGATC

Downstream 100 bases:

>100_bases
ATATCGGCCGCAAGAATCGGAGTGCTTGCGATGGAAAGCCCGCGTATTCTGGCTGTGTGATTGATGATGACGAGACTTCT
GCGATGAAGACGACCATTGC

Product: DNA-3-methyladenine glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MSGIHAKTIAADLSPLAYRRAVKFLSELDEDWALHIAAIGPCRHEAKPAREPYEALVRAIAYQQLHARAGDAILGRLLAL
YPGVSFPAPEQLLASDPELQRACGFSAEKLATIRRIAQATVEGLVPNLEEARRLPDTALIERLTSLRGVGRWTVEMFLIY
SLERSDVLPVDDFGVREGYGRLKGLERAPTPRQMREIGEAWSPFRTVAAWYLWRLPSR

Sequences:

>Translated_218_residues
MSGIHAKTIAADLSPLAYRRAVKFLSELDEDWALHIAAIGPCRHEAKPAREPYEALVRAIAYQQLHARAGDAILGRLLAL
YPGVSFPAPEQLLASDPELQRACGFSAEKLATIRRIAQATVEGLVPNLEEARRLPDTALIERLTSLRGVGRWTVEMFLIY
SLERSDVLPVDDFGVREGYGRLKGLERAPTPRQMREIGEAWSPFRTVAAWYLWRLPSR
>Mature_217_residues
SGIHAKTIAADLSPLAYRRAVKFLSELDEDWALHIAAIGPCRHEAKPAREPYEALVRAIAYQQLHARAGDAILGRLLALY
PGVSFPAPEQLLASDPELQRACGFSAEKLATIRRIAQATVEGLVPNLEEARRLPDTALIERLTSLRGVGRWTVEMFLIYS
LERSDVLPVDDFGVREGYGRLKGLERAPTPRQMREIGEAWSPFRTVAAWYLWRLPSR

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=176, Percent_Identity=27.2727272727273, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00730 HhH-GPD [H]

EC number: =3.2.2.21 [H]

Molecular weight: Translated: 24413; Mature: 24282

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGIHAKTIAADLSPLAYRRAVKFLSELDEDWALHIAAIGPCRHEAKPAREPYEALVRAI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEHHEECCHHHCCCCCCCHHHHHHHHH
AYQQLHARAGDAILGRLLALYPGVSFPAPEQLLASDPELQRACGFSAEKLATIRRIAQAT
HHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHH
VEGLVPNLEEARRLPDTALIERLTSLRGVGRWTVEMFLIYSLERSDVLPVDDFGVREGYG
HHHCCCCHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
RLKGLERAPTPRQMREIGEAWSPFRTVAAWYLWRLPSR
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SGIHAKTIAADLSPLAYRRAVKFLSELDEDWALHIAAIGPCRHEAKPAREPYEALVRAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEHHEECCHHHCCCCCCCHHHHHHHHH
AYQQLHARAGDAILGRLLALYPGVSFPAPEQLLASDPELQRACGFSAEKLATIRRIAQAT
HHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHH
VEGLVPNLEEARRLPDTALIERLTSLRGVGRWTVEMFLIYSLERSDVLPVDDFGVREGYG
HHHCCCCHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
RLKGLERAPTPRQMREIGEAWSPFRTVAAWYLWRLPSR
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]