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The map label for this gene is ada [H]

Identifier: 94312695

GI number: 94312695

Start: 303230

End: 304294

Strand: Reverse

Name: ada [H]

Synonym: Rmet_3765

Alternate gene names: 94312695

Gene position: 304294-303230 (Counterclockwise)

Preceding gene: 94312696

Following gene: 94312694

Centisome position: 11.79

GC content: 64.41

Gene sequence:

>1065_bases
ATGAAGACGACCATTGCCTACGAAACGGAAGACGACCGCTGGACGGCCGTACAGGCACGCGATGCTGGCGCCGACGGCCA
TTTCGTTTACGCCGTGCGCACCACGGGCGTGTACTGTCAACCAAGTTCGTCGGCTCGGCTGCCCAAGCGCGAGAACGTGG
AGTTCTTTGACTCGGCCGAAATCGCCGAGGCTGCGGGCTACCGCTGCAGCCGCCGTGCTCATGGTGACCAGACCAGTGCA
GCAGCCGAGCGCGCTGCATTGGTGGCCCGAGCCTGTCGCATGATTGAAGCATCGGAGACGCCCCCTCAGCTCGATGACCT
GGCGACTGCAATCGGCATGAGTCCGTTTCACTTCCATCGCCTCTTCAAGGCTGAAACGGGCTTGACGCCCAAGGCCTACA
GCTCGGCCTATCGCGCCCGCAAGCTGCGCGAAGAACTGAGCAGCCCGGAAGCGTCCATCACGAACGCTATCTACGACAGC
GGGTTCAATTCAAACAGCCGGTTCTACGAAGCCTCCGACCAGTTGCTGGGCATGCGCGCGCGGGATTATCGCTCCGGTGG
TGTCGGTGCTGTTATCCGCTTCGCCGTGGGCCAGTGCTCCCTGGGCGCCATTCTGGTTGCGCAGAGCCAGCGCGGCATCT
GTGCAATCCTGCTGGGCGACGATCCCGACCAGCTGGTGCGCGACCTCCAAGACCAATTCCGCAAGGCGGAGATCGTCGGC
TGCGACGGTGAGTTCGAGCAGTTGATTGCCCAGGTGGTGGGCTTTATCGAAGCGCCTGCGATGGGATTGCACCTGCCGTT
GGACGTACAGGGCACCGCCTTCCAGGAGCGCGTCTGGCGCGCGCTGCGCGAGATCCCGCCGGGTACGACCGTGAGCTATG
CCGAGATCGCCGAGCGCATCGGTTCACCCAAGGCGGTACGCGCCGTCGCCCAGGCGTGTGCCACGAATCACATCGCGGTG
GCTATTCCCTGCCACCGCGTCGTGCGGCGTGATGGCGATCTGGCCGGCTACCGCTGGGGCGTGGATCGCAAGCGCGAGCT
GCTGCGCCGCGAAACCCAGGTTTGA

Upstream 100 bases:

>100_bases
GCCTGCCCAGTCGATAGATATCGGCCGCAAGAATCGGAGTGCTTGCGATGGAAAGCCCGCGTATTCTGGCTGTGTGATTG
ATGATGACGAGACTTCTGCG

Downstream 100 bases:

>100_bases
TGGGAAGGTGTCCCATGACTGCGTTCGACGCAACCCTCGATCGCCTGAATTGGGTGGACATCGGCCTGCAACTCGATGCC
GAAGGCTATGCCGTGCTGCC

Product: bifunctional DNA-binding transcriptional dual regulator/O6-methylguanine-DNA methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MKTTIAYETEDDRWTAVQARDAGADGHFVYAVRTTGVYCQPSSSARLPKRENVEFFDSAEIAEAAGYRCSRRAHGDQTSA
AAERAALVARACRMIEASETPPQLDDLATAIGMSPFHFHRLFKAETGLTPKAYSSAYRARKLREELSSPEASITNAIYDS
GFNSNSRFYEASDQLLGMRARDYRSGGVGAVIRFAVGQCSLGAILVAQSQRGICAILLGDDPDQLVRDLQDQFRKAEIVG
CDGEFEQLIAQVVGFIEAPAMGLHLPLDVQGTAFQERVWRALREIPPGTTVSYAEIAERIGSPKAVRAVAQACATNHIAV
AIPCHRVVRRDGDLAGYRWGVDRKRELLRRETQV

Sequences:

>Translated_354_residues
MKTTIAYETEDDRWTAVQARDAGADGHFVYAVRTTGVYCQPSSSARLPKRENVEFFDSAEIAEAAGYRCSRRAHGDQTSA
AAERAALVARACRMIEASETPPQLDDLATAIGMSPFHFHRLFKAETGLTPKAYSSAYRARKLREELSSPEASITNAIYDS
GFNSNSRFYEASDQLLGMRARDYRSGGVGAVIRFAVGQCSLGAILVAQSQRGICAILLGDDPDQLVRDLQDQFRKAEIVG
CDGEFEQLIAQVVGFIEAPAMGLHLPLDVQGTAFQERVWRALREIPPGTTVSYAEIAERIGSPKAVRAVAQACATNHIAV
AIPCHRVVRRDGDLAGYRWGVDRKRELLRRETQV
>Mature_354_residues
MKTTIAYETEDDRWTAVQARDAGADGHFVYAVRTTGVYCQPSSSARLPKRENVEFFDSAEIAEAAGYRCSRRAHGDQTSA
AAERAALVARACRMIEASETPPQLDDLATAIGMSPFHFHRLFKAETGLTPKAYSSAYRARKLREELSSPEASITNAIYDS
GFNSNSRFYEASDQLLGMRARDYRSGGVGAVIRFAVGQCSLGAILVAQSQRGICAILLGDDPDQLVRDLQDQFRKAEIVG
CDGEFEQLIAQVVGFIEAPAMGLHLPLDVQGTAFQERVWRALREIPPGTTVSYAEIAERIGSPKAVRAVAQACATNHIAV
AIPCHRVVRRDGDLAGYRWGVDRKRELLRRETQV

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=114, Percent_Identity=32.4561403508772, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1788542, Length=345, Percent_Identity=52.1739130434783, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1787596, Length=87, Percent_Identity=48.2758620689655, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=55, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=55, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320001, Length=83, Percent_Identity=43.3734939759036, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI17137554, Length=175, Percent_Identity=33.7142857142857, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 38873; Mature: 38873

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTIAYETEDDRWTAVQARDAGADGHFVYAVRTTGVYCQPSSSARLPKRENVEFFDSAE
CCCEEEEECCCCCEEEEEECCCCCCCEEEEEEEECCEEECCCCCCCCCCCCCCCCCCCHH
IAEAAGYRCSRRAHGDQTSAAAERAALVARACRMIEASETPPQLDDLATAIGMSPFHFHR
HHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHH
LFKAETGLTPKAYSSAYRARKLREELSSPEASITNAIYDSGFNSNSRFYEASDQLLGMRA
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEECHHHHHCCHH
RDYRSGGVGAVIRFAVGQCSLGAILVAQSQRGICAILLGDDPDQLVRDLQDQFRKAEIVG
HHCCCCCHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHEEEC
CDGEFEQLIAQVVGFIEAPAMGLHLPLDVQGTAFQERVWRALREIPPGTTVSYAEIAERI
CCCHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
GSPKAVRAVAQACATNHIAVAIPCHRVVRRDGDLAGYRWGVDRKRELLRRETQV
CCCHHHHHHHHHHHCCCEEEEECHHHHHHCCCCCCCEECCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKTTIAYETEDDRWTAVQARDAGADGHFVYAVRTTGVYCQPSSSARLPKRENVEFFDSAE
CCCEEEEECCCCCEEEEEECCCCCCCEEEEEEEECCEEECCCCCCCCCCCCCCCCCCCHH
IAEAAGYRCSRRAHGDQTSAAAERAALVARACRMIEASETPPQLDDLATAIGMSPFHFHR
HHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHH
LFKAETGLTPKAYSSAYRARKLREELSSPEASITNAIYDSGFNSNSRFYEASDQLLGMRA
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEECHHHHHCCHH
RDYRSGGVGAVIRFAVGQCSLGAILVAQSQRGICAILLGDDPDQLVRDLQDQFRKAEIVG
HHCCCCCHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHEEEC
CDGEFEQLIAQVVGFIEAPAMGLHLPLDVQGTAFQERVWRALREIPPGTTVSYAEIAERI
CCCHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
GSPKAVRAVAQACATNHIAVAIPCHRVVRRDGDLAGYRWGVDRKRELLRRETQV
CCCHHHHHHHHHHHCCCEEEEECHHHHHHCCCCCCCEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]