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The map label for this gene is sbcD [H]

Identifier: 94312595

GI number: 94312595

Start: 198565

End: 199710

Strand: Direct

Name: sbcD [H]

Synonym: Rmet_3663

Alternate gene names: 94312595

Gene position: 198565-199710 (Clockwise)

Preceding gene: 94312594

Following gene: 94312596

Centisome position: 7.7

GC content: 67.54

Gene sequence:

>1146_bases
TTGCGTTTTCTCCACACCGCCGACTGGCATCTTGGTCGGCTCTTCCACGCCCGTAGCCTGATCGAGGATCAGTCTCATGT
GCTCGACCAGTTCGTCGCGCTGGTGCGCGATCTGCGCCCCGACGCCGTGCTGATTGCCGGCGACGTGTACGACCGCGCGG
TGCCGCCGCCGGAGGCCGTGGCGCTGCTCGACGATGTGCTGGCGCGTATCGTGGTGGATGCGGGCGTACCCGTGGTGATG
ATCGCAGGCAATCACGACAGCGCCCAACGCCTCGGTTTCGGCGCGCGCCTGCTGGCCGCGCGGGGTCTGCACGTGGCGGG
GCGCACCGGTCCGGAAACGTCCTGCGTGACGCTGCACGATGCGCACGGCGAGGTGCGCATCTACGCCTTGCCCTATGCCG
AACCGGCGGCGGTGCGCGACGCGCTCGGCACCGACTTGCCGACGCACGAAGCCGCATTGAACGCCCAGCTCGACGCCGTC
CGCGCCACGCATCCCGCAGGCGTCCGTTCTGTGGCCGTGGCCCATGCTTTCGTGGTGGGCGGCGCGGTCAGCGAATCGGA
GCGCCCGCTGAGCGTGGGCGGTAGCGGTGCCGTGGCCGCGGACGTGTTCCACGGCTTCGATTTCGTGGCATTGGGACATC
TGCATCGTCCGCAATCCATCGGCGCTAGGATTCACTATTCGGGGTCGCTGCTCAAGTATTCGCTGTCGGAAACCGAACAC
ATCAAGACGGTATCGCTGATCGATGTTGACGCCGAGGGTGGCGTCCGGATCGAGGCGATACCGCTGCGACCGTTGCGCGA
ACTGCGCGTGCTGACCGGCACGCTGGCCGGCTTGCTTGAGGCGGGCGCCACCGACCCGCACCGGGACGACTACGTCCATG
CGGTGCTGACCGACGCGGGCGCGTTGCTGGACCCGATGGCGCGACTGCGCCAGGTCTATCCGAACGCACTGGCGATCGAG
CGCGCGGTGCTGGCGCGCAGCGGGCAGGCCGGGGAAGCGGGGCGCAAGCTGCGGGAACTGGATACGGCCAGCCTGTTTGC
GAATTTTTTCCGCGAGGTGGCTGACACAGACCTCGACGATGATCAGCGCCGCGCGCTCGACGATCTATTGGCAGTCATGC
AGGCATCGGAAAGGGAGTCGGCATGA

Upstream 100 bases:

>100_bases
CTGAGTGGTGTCCGCGTACCGTTTGGTGGCCGCGACGTTATGATGCGGGACCGCACGGTATCTGCCGTGCTCTTGGCGCC
ACTGTGCCGAAGGACCCGAA

Downstream 100 bases:

>100_bases
AACCGCTTTACCTGAAACTGCAGGCCTTCGGCCCGTTCGCCGCCACGGAGACGATTGACTTCACGCGGCTCGGCGACCAG
GCGTTCTTCCTGATCCACGG

Product: exonuclease dsDNA SbcCD, ATP-dependent, D subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MRFLHTADWHLGRLFHARSLIEDQSHVLDQFVALVRDLRPDAVLIAGDVYDRAVPPPEAVALLDDVLARIVVDAGVPVVM
IAGNHDSAQRLGFGARLLAARGLHVAGRTGPETSCVTLHDAHGEVRIYALPYAEPAAVRDALGTDLPTHEAALNAQLDAV
RATHPAGVRSVAVAHAFVVGGAVSESERPLSVGGSGAVAADVFHGFDFVALGHLHRPQSIGARIHYSGSLLKYSLSETEH
IKTVSLIDVDAEGGVRIEAIPLRPLRELRVLTGTLAGLLEAGATDPHRDDYVHAVLTDAGALLDPMARLRQVYPNALAIE
RAVLARSGQAGEAGRKLRELDTASLFANFFREVADTDLDDDQRRALDDLLAVMQASERESA

Sequences:

>Translated_381_residues
MRFLHTADWHLGRLFHARSLIEDQSHVLDQFVALVRDLRPDAVLIAGDVYDRAVPPPEAVALLDDVLARIVVDAGVPVVM
IAGNHDSAQRLGFGARLLAARGLHVAGRTGPETSCVTLHDAHGEVRIYALPYAEPAAVRDALGTDLPTHEAALNAQLDAV
RATHPAGVRSVAVAHAFVVGGAVSESERPLSVGGSGAVAADVFHGFDFVALGHLHRPQSIGARIHYSGSLLKYSLSETEH
IKTVSLIDVDAEGGVRIEAIPLRPLRELRVLTGTLAGLLEAGATDPHRDDYVHAVLTDAGALLDPMARLRQVYPNALAIE
RAVLARSGQAGEAGRKLRELDTASLFANFFREVADTDLDDDQRRALDDLLAVMQASERESA
>Mature_381_residues
MRFLHTADWHLGRLFHARSLIEDQSHVLDQFVALVRDLRPDAVLIAGDVYDRAVPPPEAVALLDDVLARIVVDAGVPVVM
IAGNHDSAQRLGFGARLLAARGLHVAGRTGPETSCVTLHDAHGEVRIYALPYAEPAAVRDALGTDLPTHEAALNAQLDAV
RATHPAGVRSVAVAHAFVVGGAVSESERPLSVGGSGAVAADVFHGFDFVALGHLHRPQSIGARIHYSGSLLKYSLSETEH
IKTVSLIDVDAEGGVRIEAIPLRPLRELRVLTGTLAGLLEAGATDPHRDDYVHAVLTDAGALLDPMARLRQVYPNALAIE
RAVLARSGQAGEAGRKLRELDTASLFANFFREVADTDLDDDQRRALDDLLAVMQASERESA

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=403, Percent_Identity=26.5508684863524, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 40760; Mature: 40760

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00133 CARBOXYPEPT_ZN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFLHTADWHLGRLFHARSLIEDQSHVLDQFVALVRDLRPDAVLIAGDVYDRAVPPPEAV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCHHHH
ALLDDVLARIVVDAGVPVVMIAGNHDSAQRLGFGARLLAARGLHVAGRTGPETSCVTLHD
HHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCHHHHHHHCCCEEECCCCCCCCEEEEEE
AHGEVRIYALPYAEPAAVRDALGTDLPTHEAALNAQLDAVRATHPAGVRSVAVAHAFVVG
CCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
GAVSESERPLSVGGSGAVAADVFHGFDFVALGHLHRPQSIGARIHYSGSLLKYSLSETEH
CCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHCCCCCCHHCCCEEEECCCEEEEECCCCCC
IKTVSLIDVDAEGGVRIEAIPLRPLRELRVLTGTLAGLLEAGATDPHRDDYVHAVLTDAG
EEEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCH
ALLDPMARLRQVYPNALAIERAVLARSGQAGEAGRKLRELDTASLFANFFREVADTDLDD
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DQRRALDDLLAVMQASERESA
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRFLHTADWHLGRLFHARSLIEDQSHVLDQFVALVRDLRPDAVLIAGDVYDRAVPPPEAV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCHHHH
ALLDDVLARIVVDAGVPVVMIAGNHDSAQRLGFGARLLAARGLHVAGRTGPETSCVTLHD
HHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCHHHHHHHCCCEEECCCCCCCCEEEEEE
AHGEVRIYALPYAEPAAVRDALGTDLPTHEAALNAQLDAVRATHPAGVRSVAVAHAFVVG
CCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
GAVSESERPLSVGGSGAVAADVFHGFDFVALGHLHRPQSIGARIHYSGSLLKYSLSETEH
CCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHCCCCCCHHCCCEEEECCCEEEEECCCCCC
IKTVSLIDVDAEGGVRIEAIPLRPLRELRVLTGTLAGLLEAGATDPHRDDYVHAVLTDAG
EEEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCH
ALLDPMARLRQVYPNALAIERAVLARSGQAGEAGRKLRELDTASLFANFFREVADTDLDD
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DQRRALDDLLAVMQASERESA
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA