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The map label for this gene is hyfR [H]

Identifier: 94312594

GI number: 94312594

Start: 197104

End: 198468

Strand: Direct

Name: hyfR [H]

Synonym: Rmet_3662

Alternate gene names: 94312594

Gene position: 197104-198468 (Clockwise)

Preceding gene: 94312593

Following gene: 94312595

Centisome position: 7.64

GC content: 66.81

Gene sequence:

>1365_bases
ATGAAAGCAGACCGGTGGGCCTACGAGAGCCTTGAAGTCTATGTGTGGGAAGGCAAGTACGAGATCGCCGATCGCGTCGC
GCGCTTTCTGACGCCACTGGGCGCCGAAGTCGTTCGCGCAGGCGCGCTCGATGCGCTGCCGGCCGAACCACGGGTCAAGC
CCAGCATCGCCGTCATCAGTGCGTCGGCGATCGGCTCCGCCAAGTTCACGCTGGATTGGGAAGCCGCGCACGGCATGCCG
GTGATCTGGGTCGGCGCGCCCGACCGCGAGAGCGATCCCGGTCGGTTCCCGCCCGAGTACGCCTACATCCTGCCCCACGA
TTTCACCGGCGCCGATCTGCGCACGCAGATAGCCAAGCTGATGCCGCAATTGATGGCGGCCGCCGAGGCTCGCCCGGATG
TGGCCGATCTCGTCGCCGGGTCTGCGGCAATGCAGTCGCTGCTGGAGCAGGTCGATACCTTCGCCAACTTCGACAGCAAC
GTGATGCTCTATGGCGAGACCGGCGCGGGGAAGGAACGCATCGCGCGGCTCTTTCACGATCGCAACGCGACGTATGGCAA
GGGACCGTTCGTGGCGGTCAACTGCGGCGCGATTCCCGATGGGCTGTTCGAGTCGCAGTTCTTCGGTCACGCCAAGGGCG
CGTTCACCGGCGCGATGTTCGCACATCGTGGCTTCTTCGAGCAGGCCACCGGCGGCACGCTGTTCCTTGATGAGATCGGC
GACTTGCCGCTGTTCCAGCAGGTCAAGCTGCTGCGCGTGCTGGAGGAGAACGCGGTGACGCGGCTCGGCTCCGCCATGGC
GGTCAAGCTGGACTTCCGGCTGGTGGCCGCCACCAACAAGGATCTGCGCGAAGCCGTGACGCAGGGCCGCTTCCGCGCGG
ATTTGTACTTCCGGCTGGCCGTGATCGAACTGCGGCTGCCCAGCCTGGAGGACCGTGGCCCTGCGGACAAGATCGGTCTG
CTGCAATCGTTCATGCGCCACATGATCGGCGCCGCCGCCTACGATGCGCTGCCGCCGATGCCAGACTGGCTCAAGGGCGC
CGTGGGCTCCGCGTTCTTCGTCGGTAACGTGCGCGAACTGCGCAACCTGGCCGAACGCATCGGCATCACGGTCCAGCAGC
GCGGCGAATGGGACGAGGCGCGCATCCGGCCGCTGTTTCGCTCGCTGCGCCCCGGCGCGTTCGATGGCACCGAGCGGGCC
GACCCGCGCGGCGACGCGGAGGAGCGCCGCCGCATCATTGCCGCGCTCGATGCCAACAGCTGGCGACGCCAGGACACGGC
AACGTGCCTCGGCATCAGCCGCAAGGTGCTGTGGGAAAAAATGCGCAAGTTCCAGATCGAGGACAGCGAGGCGGAGCCGG
TCTGA

Upstream 100 bases:

>100_bases
CCTTGACGAGCCTGCTCGGCATCAGCCTTGCCAACGCCGACTTGTGGCTCAACGGCGTCGACTGCAACAACACGGAACTC
GTGTACTGAACTGAACGATC

Downstream 100 bases:

>100_bases
GTGGTGTCCGCGTACCGTTTGGTGGCCGCGACGTTATGATGCGGGACCGCACGGTATCTGCCGTGCTCTTGGCGCCACTG
TGCCGAAGGACCCGAATTGC

Product: putative sigma54-interaction Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MKADRWAYESLEVYVWEGKYEIADRVARFLTPLGAEVVRAGALDALPAEPRVKPSIAVISASAIGSAKFTLDWEAAHGMP
VIWVGAPDRESDPGRFPPEYAYILPHDFTGADLRTQIAKLMPQLMAAAEARPDVADLVAGSAAMQSLLEQVDTFANFDSN
VMLYGETGAGKERIARLFHDRNATYGKGPFVAVNCGAIPDGLFESQFFGHAKGAFTGAMFAHRGFFEQATGGTLFLDEIG
DLPLFQQVKLLRVLEENAVTRLGSAMAVKLDFRLVAATNKDLREAVTQGRFRADLYFRLAVIELRLPSLEDRGPADKIGL
LQSFMRHMIGAAAYDALPPMPDWLKGAVGSAFFVGNVRELRNLAERIGITVQQRGEWDEARIRPLFRSLRPGAFDGTERA
DPRGDAEERRRIIAALDANSWRRQDTATCLGISRKVLWEKMRKFQIEDSEAEPV

Sequences:

>Translated_454_residues
MKADRWAYESLEVYVWEGKYEIADRVARFLTPLGAEVVRAGALDALPAEPRVKPSIAVISASAIGSAKFTLDWEAAHGMP
VIWVGAPDRESDPGRFPPEYAYILPHDFTGADLRTQIAKLMPQLMAAAEARPDVADLVAGSAAMQSLLEQVDTFANFDSN
VMLYGETGAGKERIARLFHDRNATYGKGPFVAVNCGAIPDGLFESQFFGHAKGAFTGAMFAHRGFFEQATGGTLFLDEIG
DLPLFQQVKLLRVLEENAVTRLGSAMAVKLDFRLVAATNKDLREAVTQGRFRADLYFRLAVIELRLPSLEDRGPADKIGL
LQSFMRHMIGAAAYDALPPMPDWLKGAVGSAFFVGNVRELRNLAERIGITVQQRGEWDEARIRPLFRSLRPGAFDGTERA
DPRGDAEERRRIIAALDANSWRRQDTATCLGISRKVLWEKMRKFQIEDSEAEPV
>Mature_454_residues
MKADRWAYESLEVYVWEGKYEIADRVARFLTPLGAEVVRAGALDALPAEPRVKPSIAVISASAIGSAKFTLDWEAAHGMP
VIWVGAPDRESDPGRFPPEYAYILPHDFTGADLRTQIAKLMPQLMAAAEARPDVADLVAGSAAMQSLLEQVDTFANFDSN
VMLYGETGAGKERIARLFHDRNATYGKGPFVAVNCGAIPDGLFESQFFGHAKGAFTGAMFAHRGFFEQATGGTLFLDEIG
DLPLFQQVKLLRVLEENAVTRLGSAMAVKLDFRLVAATNKDLREAVTQGRFRADLYFRLAVIELRLPSLEDRGPADKIGL
LQSFMRHMIGAAAYDALPPMPDWLKGAVGSAFFVGNVRELRNLAERIGITVQQRGEWDEARIRPLFRSLRPGAFDGTERA
DPRGDAEERRRIIAALDANSWRRQDTATCLGISRKVLWEKMRKFQIEDSEAEPV

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=360, Percent_Identity=40.5555555555556, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1788550, Length=322, Percent_Identity=38.8198757763975, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1790437, Length=307, Percent_Identity=37.785016286645, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1788905, Length=233, Percent_Identity=43.7768240343348, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1786524, Length=331, Percent_Identity=37.1601208459215, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1789087, Length=231, Percent_Identity=44.5887445887446, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1789233, Length=342, Percent_Identity=34.7953216374269, Blast_Score=178, Evalue=6e-46,
Organism=Escherichia coli, GI1790299, Length=339, Percent_Identity=33.9233038348083, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI87082152, Length=276, Percent_Identity=38.4057971014493, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI87081872, Length=231, Percent_Identity=39.3939393939394, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1787583, Length=357, Percent_Identity=31.6526610644258, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1789828, Length=244, Percent_Identity=36.0655737704918, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI87081858, Length=297, Percent_Identity=30.3030303030303, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50221; Mature: 50221

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKADRWAYESLEVYVWEGKYEIADRVARFLTPLGAEVVRAGALDALPAEPRVKPSIAVIS
CCCCHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
ASAIGSAKFTLDWEAAHGMPVIWVGAPDRESDPGRFPPEYAYILPHDFTGADLRTQIAKL
ECCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHH
MPQLMAAAEARPDVADLVAGSAAMQSLLEQVDTFANFDSNVMLYGETGAGKERIARLFHD
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHH
RNATYGKGPFVAVNCGAIPDGLFESQFFGHAKGAFTGAMFAHRGFFEQATGGTLFLDEIG
CCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCHHHCCCCEEEHHCCC
DLPLFQQVKLLRVLEENAVTRLGSAMAVKLDFRLVAATNKDLREAVTQGRFRADLYFRLA
CCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEECCCHHHHHHHHCCCHHHHHEEEEE
VIELRLPSLEDRGPADKIGLLQSFMRHMIGAAAYDALPPMPDWLKGAVGSAFFVGNVREL
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCEEEECCHHHH
RNLAERIGITVQQRGEWDEARIRPLFRSLRPGAFDGTERADPRGDAEERRRIIAALDANS
HHHHHHHCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHEEEEECCCC
WRRQDTATCLGISRKVLWEKMRKFQIEDSEAEPV
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKADRWAYESLEVYVWEGKYEIADRVARFLTPLGAEVVRAGALDALPAEPRVKPSIAVIS
CCCCHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
ASAIGSAKFTLDWEAAHGMPVIWVGAPDRESDPGRFPPEYAYILPHDFTGADLRTQIAKL
ECCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHH
MPQLMAAAEARPDVADLVAGSAAMQSLLEQVDTFANFDSNVMLYGETGAGKERIARLFHD
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHH
RNATYGKGPFVAVNCGAIPDGLFESQFFGHAKGAFTGAMFAHRGFFEQATGGTLFLDEIG
CCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCHHHCCCCEEEHHCCC
DLPLFQQVKLLRVLEENAVTRLGSAMAVKLDFRLVAATNKDLREAVTQGRFRADLYFRLA
CCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEECCCHHHHHHHHCCCHHHHHEEEEE
VIELRLPSLEDRGPADKIGLLQSFMRHMIGAAAYDALPPMPDWLKGAVGSAFFVGNVREL
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCEEEECCHHHH
RNLAERIGITVQQRGEWDEARIRPLFRSLRPGAFDGTERADPRGDAEERRRIIAALDANS
HHHHHHHCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHEEEEECCCC
WRRQDTATCLGISRKVLWEKMRKFQIEDSEAEPV
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]